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The map label for this gene is nei_2

Identifier: 121639220

GI number: 121639220

Start: 3669891

End: 3670658

Strand: Direct

Name: nei_2

Synonym: BCG_3362

Alternate gene names: 121639220

Gene position: 3669891-3670658 (Clockwise)

Preceding gene: 121639219

Following gene: 121639227

Centisome position: 83.89

GC content: 67.97

Gene sequence:

>768_bases
ATGCCCGAGGGCGACACCGTCTGGCACACCGCGGCCACGTTGCGGCGGCATCTGGCCGGTCGCACGTTGACACGTTGCGA
CATCCGAGTGCCACGGTTTGCCGCCGTCGACCTCACCGGCGAGGTAGTGGACGAGGTGATCAGTCGGGGCAAGCACCTGT
TCATCCGAACCGGGACAGCCAGCATTCATTCGCATCTGCAGATGGACGGCAGCTGGCGGGTCGGCAACCGGCCGGTGCGG
GTGGATCATCGGGCGCGAATCATTTTGGAAGCCAACCAGCAAGAACAGGCCATCCGGGTGGTCGGCGTCGACCTAGGCCT
GTTGGAGGTCATCGACCGGCACAACGACGGCGCCGTCGTCGCACACCTAGGACCTGATCTGCTGGCCGACGATTGGGACC
CGCAGCGTGCAGCCGCCAACCTGATCGTTGCCCCGGACCGGCCCATCGCCGAGGCACTGCTCGACCAGCGGGTGCTCGCC
GGGATCGGCAACGTGTATTGCAACGAACTGTGCTTCGTCAGCGGAGTATTGCCGACGGCCCCGGTGAGCGCGGTCGCCGA
CCCGCGCCGCCTGGTCACCCGCGCCCGAGACATGCTGTGGGTCAACCGCTTCCGCTGGAATCGGTGCACCACTGGCGATA
CCCGGGCCGGCCGGCGACTGTGGGTCTACGGGCGGGCCGGGCAGGGTTGCCGCCGCTGCGGCACGCTCATCGCCTACGAC
ACTACCGACGAGCGGGTGCGGTATTGGTGCCCGGCCTGCCAGCGCTGA

Upstream 100 bases:

>100_bases
GCCGGTGCTGCAGCCCGGCGGGCGGGCGTCGGCGGCACTGACGGCGCTGCTGGCAGCCGGCTTCGTCCGCACACCTCGCG
GTCTGCGGCGGCGGTAAGCC

Downstream 100 bases:

>100_bases
ACCGGGCGATCAAAGCCAGCACCTAGTCGCGGCCGTGGGTAGCGAAGAACTGGGCAATGACTTGCGACCCGTCGAACGCG
CGCGTGGTCGCCCCGATGAC

Product: putative endonuclease VIII nei

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv3297/MT3396; Putative AP lyase Rv3297/MT3396

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPEGDTVWHTAATLRRHLAGRTLTRCDIRVPRFAAVDLTGEVVDEVISRGKHLFIRTGTASIHSHLQMDGSWRVGNRPVR
VDHRARIILEANQQEQAIRVVGVDLGLLEVIDRHNDGAVVAHLGPDLLADDWDPQRAAANLIVAPDRPIAEALLDQRVLA
GIGNVYCNELCFVSGVLPTAPVSAVADPRRLVTRARDMLWVNRFRWNRCTTGDTRAGRRLWVYGRAGQGCRRCGTLIAYD
TTDERVRYWCPACQR

Sequences:

>Translated_255_residues
MPEGDTVWHTAATLRRHLAGRTLTRCDIRVPRFAAVDLTGEVVDEVISRGKHLFIRTGTASIHSHLQMDGSWRVGNRPVR
VDHRARIILEANQQEQAIRVVGVDLGLLEVIDRHNDGAVVAHLGPDLLADDWDPQRAAANLIVAPDRPIAEALLDQRVLA
GIGNVYCNELCFVSGVLPTAPVSAVADPRRLVTRARDMLWVNRFRWNRCTTGDTRAGRRLWVYGRAGQGCRRCGTLIAYD
TTDERVRYWCPACQR
>Mature_254_residues
PEGDTVWHTAATLRRHLAGRTLTRCDIRVPRFAAVDLTGEVVDEVISRGKHLFIRTGTASIHSHLQMDGSWRVGNRPVRV
DHRARIILEANQQEQAIRVVGVDLGLLEVIDRHNDGAVVAHLGPDLLADDWDPQRAAANLIVAPDRPIAEALLDQRVLAG
IGNVYCNELCFVSGVLPTAPVSAVADPRRLVTRARDMLWVNRFRWNRCTTGDTRAGRRLWVYGRAGQGCRRCGTLIAYDT
TDERVRYWCPACQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Homo sapiens, GI157388969, Length=220, Percent_Identity=24.0909090909091, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1786932, Length=270, Percent_Identity=28.1481481481481, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790066, Length=274, Percent_Identity=26.6423357664234, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y3297_MYCTU (P64156)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   B70982
- RefSeq:   NP_217814.1
- RefSeq:   NP_337925.1
- ProteinModelPortal:   P64156
- SMR:   P64156
- EnsemblBacteria:   EBMYCT00000002647
- EnsemblBacteria:   EBMYCT00000071901
- GeneID:   887937
- GeneID:   926657
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3396
- KEGG:   mtu:Rv3297
- TIGR:   MT3396
- TubercuList:   Rv3297
- GeneTree:   EBGT00050000016359
- HOGENOM:   HBG690070
- OMA:   RRCGTRI
- ProtClustDB:   CLSK792413
- BRENDA:   4.2.99.18
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- SMART:   SM00898

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =4.2.99.18

Molecular weight: Translated: 28526; Mature: 28395

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 51-51 ACT_SITE 245-245 BINDING 67-67 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDTVWHTAATLRRHLAGRTLTRCDIRVPRFAAVDLTGEVVDEVISRGKHLFIRTGTA
CCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHHHCCCEEEEEECCH
SIHSHLQMDGSWRVGNRPVRVDHRARIILEANQQEQAIRVVGVDLGLLEVIDRHNDGAVV
HHHHHEEECCCEEECCCCEEECCCEEEEEECCCCCCEEEEEECCHHHHHHHHCCCCCEEE
AHLGPDLLADDWDPQRAAANLIVAPDRPIAEALLDQRVLAGIGNVYCNELCFVSGVLPTA
EECCCCHHCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVSAVADPRRLVTRARDMLWVNRFRWNRCTTGDTRAGRRLWVYGRAGQGCRRCGTLIAYD
CHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCEEEEC
TTDERVRYWCPACQR
CCCHHHEECCCCCCC
>Mature Secondary Structure 
PEGDTVWHTAATLRRHLAGRTLTRCDIRVPRFAAVDLTGEVVDEVISRGKHLFIRTGTA
CCCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHHHCCCEEEEEECCH
SIHSHLQMDGSWRVGNRPVRVDHRARIILEANQQEQAIRVVGVDLGLLEVIDRHNDGAVV
HHHHHEEECCCEEECCCCEEECCCEEEEEECCCCCCEEEEEECCHHHHHHHHCCCCCEEE
AHLGPDLLADDWDPQRAAANLIVAPDRPIAEALLDQRVLAGIGNVYCNELCFVSGVLPTA
EECCCCHHCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVSAVADPRRLVTRARDMLWVNRFRWNRCTTGDTRAGRRLWVYGRAGQGCRRCGTLIAYD
CHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCEEEEC
TTDERVRYWCPACQR
CCCHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036