Click here to switch to the map view.

The map label for this gene is 121638165

Identifier: 121638165

GI number: 121638165

Start: 2534567

End: 2535904

Strand: Direct

Name: 121638165

Synonym: BCG_2300

Alternate gene names: NA

Gene position: 2534567-2535904 (Clockwise)

Preceding gene: 121638164

Following gene: 121638168

Centisome position: 57.94

GC content: 65.62

Gene sequence:

>1338_bases
GTGAAGCTGCTCAGCCCGCTGGATCAGATGTTCGCGCGCATGGAGGCGCCGCGCACGCCAATGCACATCGGCGCGTTTGC
GGTCTTCGACCTGCCTAAGGGAGCACCGCGCAGGTTCATCCGCGACCTGTACGAGGCGATCTCACAACTGGCGTTCCTGC
CCTTCCCGTTCGACAGCGTGATCGCCGGCGGCGCGTCGATGGCGTACTGGAGGCAGGTGCAGCCCGATCCGAGCTACCAC
GTCCGCTTGTCCGCCCTACCTTATCCGGGGACCGGCCGCGATCTCGGCGCGTTGGTCGAGCGGCTGCATTCGACCCCACT
TGACATGGCCAAGCCGCTATGGGAGTTGCACCTCATCGAGGGGCTAACCGGCCGTCAGTTCGCCATGTACTTCAAGGCCC
ACCACTGCGCGGTCGACGGATTGGGTGGGGTGAACCTGATCAAGAGCTGGCTCACCACCGATCCCGAGGCACCCCCAGGC
TCGGGCAAGCCCGAGCCGTTCGGCGATGACTACGACTTGGCCAGCGTGTTGGCCGCCGCCACGACGAAGCGGGCGGTCGA
GGGCGTTTCCGCGGTCAGCGAACTGGCCGGAAGGCTATCCAGCATGGTGCTGGGCGCCAACAGCTCGGTGCGGGCGGCCC
TCACCACCCCGCGTACCCCGTTTAACACCCGCGTCAACCGGCATCGACGGCTAGCGGTGCAAGTGCTGAAACTGCCGCGC
CTCAAGGCAGTGGCCCACGCCACCGACTGCACCGTCAACGACGTGATCCTGGCGTCTGTCGGCGGGGCTTGCCGACGCTA
CCTGCAGGAGCTGGGCGACCTGCCGACGAACACCCTGACCGCCTCGGTGCCGGTCGGCTTCGAGCGCGACGCAGACACGG
TCAACGCCGCCTCGGGTTTCGTCGCGCCGCTGGGCACCTCGATCGAAGACCCGGTTGCGCGGCTGACCACAATCTCGGCG
TCGACCACCCGCGGCAAGGCCGAACTGCTGGCGATGTCACCAAATGCCTTGCAGCACTACTCCGTATTCGGCTTGCTGCC
GATCGCGGTGGGGCAGAAGACCGGCGCACTCGGGGTGATTCCACCGCTGTTCAACTTCACCGTCTCCAATGTGGTGCTCT
CGAAGGACCCGTTGTATCTTTCGGGCGCCAAGCTGGATGTGATTGTTCCGATGTCGTTCCTGTGTGACGGCTATGGCCTC
AACGTGACGCTGGTCGGCTACACGGACAAGGTCGTCCTCGGCTTTCTGGGCTGCCGTGACACCTTGCCGCATCTGCAGCG
GCTAGCGCAGTACACCGGCGCGGCATTCGAGGAACTCGAGACCGCCGCCTTGCCATAG

Upstream 100 bases:

>100_bases
TCCTGGCCGAGGTCTACGCAACGCAACACGCGGGCAGTGAGCCGGGCCCCGCGGTTGCGATCCCATAGCTTTTGGGGTTG
AGGTCGCTAGGGTTGGCCTT

Downstream 100 bases:

>100_bases
CGACCAAACGACGACAACGCTCCGCCCATCGCCGGCAGTACCCGCCAATCACCACGGTGTAGCCGCTCAGGAGCGGCCCG
CCAGCCGGTCGATATCAACG

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv2285/MT2343

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKLLSPLDQMFARMEAPRTPMHIGAFAVFDLPKGAPRRFIRDLYEAISQLAFLPFPFDSVIAGGASMAYWRQVQPDPSYH
VRLSALPYPGTGRDLGALVERLHSTPLDMAKPLWELHLIEGLTGRQFAMYFKAHHCAVDGLGGVNLIKSWLTTDPEAPPG
SGKPEPFGDDYDLASVLAAATTKRAVEGVSAVSELAGRLSSMVLGANSSVRAALTTPRTPFNTRVNRHRRLAVQVLKLPR
LKAVAHATDCTVNDVILASVGGACRRYLQELGDLPTNTLTASVPVGFERDADTVNAASGFVAPLGTSIEDPVARLTTISA
STTRGKAELLAMSPNALQHYSVFGLLPIAVGQKTGALGVIPPLFNFTVSNVVLSKDPLYLSGAKLDVIVPMSFLCDGYGL
NVTLVGYTDKVVLGFLGCRDTLPHLQRLAQYTGAAFEELETAALP

Sequences:

>Translated_445_residues
MKLLSPLDQMFARMEAPRTPMHIGAFAVFDLPKGAPRRFIRDLYEAISQLAFLPFPFDSVIAGGASMAYWRQVQPDPSYH
VRLSALPYPGTGRDLGALVERLHSTPLDMAKPLWELHLIEGLTGRQFAMYFKAHHCAVDGLGGVNLIKSWLTTDPEAPPG
SGKPEPFGDDYDLASVLAAATTKRAVEGVSAVSELAGRLSSMVLGANSSVRAALTTPRTPFNTRVNRHRRLAVQVLKLPR
LKAVAHATDCTVNDVILASVGGACRRYLQELGDLPTNTLTASVPVGFERDADTVNAASGFVAPLGTSIEDPVARLTTISA
STTRGKAELLAMSPNALQHYSVFGLLPIAVGQKTGALGVIPPLFNFTVSNVVLSKDPLYLSGAKLDVIVPMSFLCDGYGL
NVTLVGYTDKVVLGFLGCRDTLPHLQRLAQYTGAAFEELETAALP
>Mature_445_residues
MKLLSPLDQMFARMEAPRTPMHIGAFAVFDLPKGAPRRFIRDLYEAISQLAFLPFPFDSVIAGGASMAYWRQVQPDPSYH
VRLSALPYPGTGRDLGALVERLHSTPLDMAKPLWELHLIEGLTGRQFAMYFKAHHCAVDGLGGVNLIKSWLTTDPEAPPG
SGKPEPFGDDYDLASVLAAATTKRAVEGVSAVSELAGRLSSMVLGANSSVRAALTTPRTPFNTRVNRHRRLAVQVLKLPR
LKAVAHATDCTVNDVILASVGGACRRYLQELGDLPTNTLTASVPVGFERDADTVNAASGFVAPLGTSIEDPVARLTTISA
STTRGKAELLAMSPNALQHYSVFGLLPIAVGQKTGALGVIPPLFNFTVSNVVLSKDPLYLSGAKLDVIVPMSFLCDGYGL
NVTLVGYTDKVVLGFLGCRDTLPHLQRLAQYTGAAFEELETAALP

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2285_MYCTU (P67206)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   A70732
- RefSeq:   NP_216801.1
- RefSeq:   NP_336813.1
- ProteinModelPortal:   P67206
- EnsemblBacteria:   EBMYCT00000002707
- EnsemblBacteria:   EBMYCT00000072887
- GeneID:   888632
- GeneID:   924080
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2343
- KEGG:   mtu:Rv2285
- TIGR:   MT2343
- TubercuList:   Rv2285
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   FNTRINR
- ProtClustDB:   CLSK791751
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 47708; Mature: 47708

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLSPLDQMFARMEAPRTPMHIGAFAVFDLPKGAPRRFIRDLYEAISQLAFLPFPFDSV
CCCCCHHHHHHHHHCCCCCCCEECEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHH
IAGGASMAYWRQVQPDPSYHVRLSALPYPGTGRDLGALVERLHSTPLDMAKPLWELHLIE
HHCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
GLTGRQFAMYFKAHHCAVDGLGGVNLIKSWLTTDPEAPPGSGKPEPFGDDYDLASVLAAA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TTKRAVEGVSAVSELAGRLSSMVLGANSSVRAALTTPRTPFNTRVNRHRRLAVQVLKLPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCH
LKAVAHATDCTVNDVILASVGGACRRYLQELGDLPTNTLTASVPVGFERDADTVNAASGF
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHCCCCC
VAPLGTSIEDPVARLTTISASTTRGKAELLAMSPNALQHYSVFGLLPIAVGQKTGALGVI
HHCCCCCCCCHHHHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
PPLFNFTVSNVVLSKDPLYLSGAKLDVIVPMSFLCDGYGLNVTLVGYTDKVVLGFLGCRD
CHHHHHHHHHEEEECCCCEEECCCEEEEECHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
TLPHLQRLAQYTGAAFEELETAALP
HHHHHHHHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MKLLSPLDQMFARMEAPRTPMHIGAFAVFDLPKGAPRRFIRDLYEAISQLAFLPFPFDSV
CCCCCHHHHHHHHHCCCCCCCEECEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHH
IAGGASMAYWRQVQPDPSYHVRLSALPYPGTGRDLGALVERLHSTPLDMAKPLWELHLIE
HHCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
GLTGRQFAMYFKAHHCAVDGLGGVNLIKSWLTTDPEAPPGSGKPEPFGDDYDLASVLAAA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TTKRAVEGVSAVSELAGRLSSMVLGANSSVRAALTTPRTPFNTRVNRHRRLAVQVLKLPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCH
LKAVAHATDCTVNDVILASVGGACRRYLQELGDLPTNTLTASVPVGFERDADTVNAASGF
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHCCCCC
VAPLGTSIEDPVARLTTISASTTRGKAELLAMSPNALQHYSVFGLLPIAVGQKTGALGVI
HHCCCCCCCCHHHHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
PPLFNFTVSNVVLSKDPLYLSGAKLDVIVPMSFLCDGYGLNVTLVGYTDKVVLGFLGCRD
CHHHHHHHHHEEEECCCCEEECCCEEEEECHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
TLPHLQRLAQYTGAAFEELETAALP
HHHHHHHHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036