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The map label for this gene is cobD

Identifier: 121638118

GI number: 121638118

Start: 2487079

End: 2488020

Strand: Reverse

Name: cobD

Synonym: BCG_2253c

Alternate gene names: 121638118

Gene position: 2488020-2487079 (Counterclockwise)

Preceding gene: 121638120

Following gene: 121638114

Centisome position: 56.88

GC content: 71.87

Gene sequence:

>942_bases
GTGTTTGCATCGATCTGGCAGACCAGGGCCGTCGGCGTGCTGATCGGCTGCCTGCTCGACGTCGTGTTCGGCGACCCCAA
ACGAGGTCATCCGGTGGCCCTGTTCGGTCGGGCGGCCGCCAAGCTGGAGCAGATCACCTACCGTGACGGCCGGGTCGCCG
GTGCGGTACACGTCGGCCTGCTGGTCGGCGCGGTGGGCTTGCTCGGCGCGGCACTGCAGCGGCTACCCGGCAGGTGCTGG
CCCGTGGCGGCCACCGCGACGGCCACCTGGGCAGCGCTGGGCGGAACTTCGCTGGCGCGCACCGGCCGCCAGATATCGGA
CCTGTTGGAGCGCGACGATGTCGAGGCGGCGCGACGGCTGCTGCCGTCGCTGTGCGGGCGTGACCCGGCCCAGCTGGGCG
GCCCGGGCCTGACGCGTGCCGCGCTGGAGTCGGTGGCCGAAAACACCGCCGACGCCCAGGTGGTGCCGCTGCTGTGGGCG
GCTTCGAGCGGCGTGCCTGCGGTGCTGGGATATCGTGCCATCAACACCCTGGACTCGATGATCGGCTACCGCTCGCCGCG
TTATCTCCGATTCGGTTGGGCTGCAGCACGATTGGATGACTGGGCCAACTATGTTGGCGCACGGGCGACGGCGGTGCTGG
TGGTGATCTGCGCGCCGGTGGTCGGTGGGTCGCCCCGCGGTGCGGTACGGGCCTGGCGGCGCGACGCCGCCCGCCATCCC
AGCCCCAACGCCGGTGTCGTCGAGGCGGCGTTTGCTGGGGCGCTTGACGTGCGGCTGGGTGGGCCCACCCGGTACCACCA
TGAGTTGCAGATCCGGCCCACCTTGGGCGACGGCCGGTCGCCCAAGGTGGCCGATCTGCGCCGCGCCGTGGTGCTGTCGC
GGGTGGTCCAGGCGGGGGCTGCGGTATTGGCCGTGATGTTGGTTTACCGCCGGCGGCCGTAG

Upstream 100 bases:

>100_bases
AACAGGTCGGCAACCCGGGTAGCTGGCCGCTTCATCGTGGATAGCTGCCGTATGAGGTCATAAATAGCTGAGCGTAGACC
ACGTGTGCGACGCTGCCGGA

Downstream 100 bases:

>100_bases
CGGTCAGCGAGTTTCTGCTCGACCGTCATTGGCTTGTCCGGTGGTGGCGACCCCGCTTTTTCCCGGCGGCGCGCCCGGAT
CTCGGCGTAGGCGAAATAGC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MFASIWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRCW
PVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWA
ASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP

Sequences:

>Translated_313_residues
MFASIWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRCW
PVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWA
ASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP
>Mature_313_residues
MFASIWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRCW
PVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWA
ASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_MYCBO (Q7VEN1)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855909.1
- EnsemblBacteria:   EBMYCT00000014560
- GeneID:   1091397
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2260c
- GeneTree:   EBGT00050000016280
- HOGENOM:   HBG647131
- OMA:   RWHPLVG
- ProtClustDB:   PRK01209
- BioCyc:   MBOV233413:MB2260C-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 33005; Mature: 33005

Theoretical pI: Translated: 11.31; Mature: 11.31

Prosite motif: PS00139 THIOL_PROTEASE_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16eef070)-; HASH(0x164ecadc)-; HASH(0x162fa3c4)-; HASH(0x1440e7e0)-; HASH(0x16d618f0)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASIWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHH
LVGAVGLLGAALQRLPGRCWPVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRL
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
LPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWAASSGVPAVLGYRAINTLDSM
HHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHH
IGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGA
CCCCCHHHHHHHCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AVLAVMLVYRRRP
HHHHHHHHHHCCH
>Mature Secondary Structure
MFASIWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHH
LVGAVGLLGAALQRLPGRCWPVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRL
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
LPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWAASSGVPAVLGYRAINTLDSM
HHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHH
IGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGA
CCCCCHHHHHHHCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AVLAVMLVYRRRP
HHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972