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The map label for this gene is gph [C]

Identifier: 121638115

GI number: 121638115

Start: 2484923

End: 2485798

Strand: Direct

Name: gph [C]

Synonym: BCG_2250

Alternate gene names: 121638115

Gene position: 2484923-2485798 (Clockwise)

Preceding gene: 121638110

Following gene: 121638116

Centisome position: 56.8

GC content: 68.04

Gene sequence:

>876_bases
GTGTCTTCGCCTCGTGAACGCCGACCCGCTTCGCAGGCGCCCAGACTTTCGCGTCGACCACCTGCTCACCAAACTTCGCG
ATCATCGCCTGATACCACAGCGCCAACGGGTAGCGGTTTGTCCAACCGCTTCGTCAACGACAATGGGATCGTGACCGACA
CGACCGCGAGCGGGACCAATTGCCCGCCTCCTCCACGCGCCGCCGCACGGCGCGCATCGTCGCCGGGTGAATCGCCGCAG
CTGGTGATCTTCGATCTGGACGGCACGCTGACCGACTCGGCGCGCGGAATCGTATCCAGCTTCCGACACGCGCTCAACCA
CATCGGTGCCCCAGTACCCGAAGGCGACCTGGCCACTCACATCGTCGGCCCGCCCATGCATGAGACGCTGCGCGCCATGG
GGCTCGGCGAATCCGCCGAGGAGGCGATCGTAGCCTACCGGGCCGACTACAGCGCCCGCGGTTGGGCGATGAACAGCTTG
TTCGACGGGATCGGGCCGCTGCTGGCCGACCTGCGCACCGCCGGTGTCCGGCTGGCCGTCGCCACCTCCAAGGCAGAGCC
GACCGCACGGCGAATCCTGCGCCACTTCGGAATTGAGCAGCACTTCGAGGTCATCGCGGGCGCGAGCACCGATGGCTCGC
GAGGCAGCAAGGTCGACGTGCTGGCCCACGCGCTCGCGCAGCTGCGGCCGCTACCCGAGCGGTTGGTGATGGTCGGCGAC
CGCAGCCACGACGTCGACGGGGCGGCCGCGCACGGCATCGACACGGTGGTGGTCGGCTGGGGCTACGGGCGCGCCGACTT
TATCGACAAGACCTCCACCACCGTCGTGACGCATGCCGCCACGATTGACGAGCTGAGGGAGGCGCTAGGTGTCTGA

Upstream 100 bases:

>100_bases
CTCATGAGGATGCAGCGGCACAAGCTTTGCTACCGGCTCGCCGCGGCGGGCAATCTCAACCTCTGCCCGCCGTAGACGAG
CCGCAGCAGCTCGGACAGGC

Downstream 100 bases:

>100_bases
TCCGCTGCACGTCACATTCGTTTGTACGGGCAACATCTGCCGGTCGCCAATGGCCGAGAAGATGTTCGCCCAACAGCTTC
GCCACCGTGGCCTGGGTGAC

Product: hypothetical protein

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSSPRERRPASQAPRLSRRPPAHQTSRSSPDTTAPTGSGLSNRFVNDNGIVTDTTASGTNCPPPPRAAARRASSPGESPQ
LVIFDLDGTLTDSARGIVSSFRHALNHIGAPVPEGDLATHIVGPPMHETLRAMGLGESAEEAIVAYRADYSARGWAMNSL
FDGIGPLLADLRTAGVRLAVATSKAEPTARRILRHFGIEQHFEVIAGASTDGSRGSKVDVLAHALAQLRPLPERLVMVGD
RSHDVDGAAAHGIDTVVVGWGYGRADFIDKTSTTVVTHAATIDELREALGV

Sequences:

>Translated_291_residues
MSSPRERRPASQAPRLSRRPPAHQTSRSSPDTTAPTGSGLSNRFVNDNGIVTDTTASGTNCPPPPRAAARRASSPGESPQ
LVIFDLDGTLTDSARGIVSSFRHALNHIGAPVPEGDLATHIVGPPMHETLRAMGLGESAEEAIVAYRADYSARGWAMNSL
FDGIGPLLADLRTAGVRLAVATSKAEPTARRILRHFGIEQHFEVIAGASTDGSRGSKVDVLAHALAQLRPLPERLVMVGD
RSHDVDGAAAHGIDTVVVGWGYGRADFIDKTSTTVVTHAATIDELREALGV
>Mature_290_residues
SSPRERRPASQAPRLSRRPPAHQTSRSSPDTTAPTGSGLSNRFVNDNGIVTDTTASGTNCPPPPRAAARRASSPGESPQL
VIFDLDGTLTDSARGIVSSFRHALNHIGAPVPEGDLATHIVGPPMHETLRAMGLGESAEEAIVAYRADYSARGWAMNSLF
DGIGPLLADLRTAGVRLAVATSKAEPTARRILRHFGIEQHFEVIAGASTDGSRGSKVDVLAHALAQLRPLPERLVMVGDR
SHDVDGAAAHGIDTVVVGWGYGRADFIDKTSTTVVTHAATIDELREALGV

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2232_MYCTU (P68911)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   D70777
- RefSeq:   NP_216748.2
- RefSeq:   NP_336762.1
- ProteinModelPortal:   P68911
- SMR:   P68911
- EnsemblBacteria:   EBMYCT00000003534
- EnsemblBacteria:   EBMYCT00000072895
- GeneID:   887597
- GeneID:   924134
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2292
- KEGG:   mtu:Rv2232
- TIGR:   MT2292
- TubercuList:   Rv2232
- GeneTree:   EBGT00050000017223
- HOGENOM:   HBG742904
- OMA:   KEEIVMI
- ProtClustDB:   CLSK799885
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01549

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 30695; Mature: 30563

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRERRPASQAPRLSRRPPAHQTSRSSPDTTAPTGSGLSNRFVNDNGIVTDTTASGTN
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCC
CPPPPRAAARRASSPGESPQLVIFDLDGTLTDSARGIVSSFRHALNHIGAPVPEGDLATH
CCCCCHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
IVGPPMHETLRAMGLGESAEEAIVAYRADYSARGWAMNSLFDGIGPLLADLRTAGVRLAV
ECCCCHHHHHHHHCCCCCHHHHHEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEE
ATSKAEPTARRILRHFGIEQHFEVIAGASTDGSRGSKVDVLAHALAQLRPLPERLVMVGD
EECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEECC
RSHDVDGAAAHGIDTVVVGWGYGRADFIDKTSTTVVTHAATIDELREALGV
CCCCCCCHHHCCCCEEEEECCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSPRERRPASQAPRLSRRPPAHQTSRSSPDTTAPTGSGLSNRFVNDNGIVTDTTASGTN
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCC
CPPPPRAAARRASSPGESPQLVIFDLDGTLTDSARGIVSSFRHALNHIGAPVPEGDLATH
CCCCCHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
IVGPPMHETLRAMGLGESAEEAIVAYRADYSARGWAMNSLFDGIGPLLADLRTAGVRLAV
ECCCCHHHHHHHHCCCCCHHHHHEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEE
ATSKAEPTARRILRHFGIEQHFEVIAGASTDGSRGSKVDVLAHALAQLRPLPERLVMVGD
EECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEECC
RSHDVDGAAAHGIDTVVVGWGYGRADFIDKTSTTVVTHAATIDELREALGV
CCCCCCCHHHCCCCEEEEECCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036