Click here to switch to the map view.

The map label for this gene is cobS

Identifier: 121638089

GI number: 121638089

Start: 2451137

End: 2451886

Strand: Direct

Name: cobS

Synonym: BCG_2224

Alternate gene names: 121638089

Gene position: 2451137-2451886 (Clockwise)

Preceding gene: 121638088

Following gene: 121638090

Centisome position: 56.03

GC content: 71.47

Gene sequence:

>750_bases
GTGATGCGTTCGCTGGCAACAGCTTTCGCATTCGCAACGGTGATACCCACACCGGGCTCAGCGACCACCCCGATGGGCCG
TGGCCCGATGACCGCGCTGCCGGTGGTGGGCGCGGCGCTGGGTGCACTGGCGGCGGCGATCGCATGGGCTGGCGCGCAAG
TGTTCGGCCCGTCCAGCCCGCTGTCCGGCATGCTCACGGTGGCGGTACTGCTGGTCGTCACTCGAGGCCTGCACATCGAT
GGCGTTGCCGATACCGCTGACGGACTGGGCTGCTATGGGCCGCCGCAGCGTGCGCTTGCGGTGATGCGCGACGGGTCGAC
CGGACCGTTCGGGGTGGCGGCCGTGGTCTTGGTCATCGCCTTGCAGGGCCTGGCCTTCGCGACCCTCACCACGGTCGGGA
TCGCTGGGATCACGCTGGCGGTCTTATCCGGCCGGGTCACCGCCGTACTGGTCTGTCGCCGGTCGGTGCCGGCAGCCCAC
GGCAGCACCCTGGGCTCGCGGGTCGCCGGTACGCAACCCGCGCCGGTGGTGGCGGCCTGGCTCGCCGTCCTGCTCGCCGT
TTCGGTGCCGGCCGGTCCCCGGCCTTGGCAAGGACCGATAGCGGTTCTGGTAGCGGTGACGGCCGGCGCGGCCCTGGCGG
CGCATTGCGTGCACCGGTTCGGCGGTGTCACCGGTGACGTGCTGGGCAGCGCGATCGAGCTGAGCACGACGGTCAGCGCC
GTGACGCTTGCGGGCTTGGCCCGGCTTTAG

Upstream 100 bases:

>100_bases
CGGTCGCGGCGCTGTCGTCGATGGCGACCTTCACCGAGGCCGGCGTGTCCACCCGGTCCGTCGACGGTGTCGACCGGACC
GCACCCCCGGCAGTCTCACC

Downstream 100 bases:

>100_bases
CAGGCGGCGAGCGGGACGCTGCAGTAGACTCATGTCCGCCGTCCCTTCCAACACAGGGCTCCCCTCCGTGTCCCCAGATT
AGGGGACATGAAATTCAACC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSPLSGMLTVAVLLVVTRGLHID
GVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIALQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRSVPAAH
GSTLGSRVAGTQPAPVVAAWLAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
VTLAGLARL

Sequences:

>Translated_249_residues
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSPLSGMLTVAVLLVVTRGLHID
GVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIALQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRSVPAAH
GSTLGSRVAGTQPAPVVAAWLAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
VTLAGLARL
>Mature_249_residues
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSPLSGMLTVAVLLVVTRGLHID
GVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIALQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRSVPAAH
GSTLGSRVAGTQPAPVVAAWLAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
VTLAGLARL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=250, Percent_Identity=31.2, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_MYCBO (Q7VEN6)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855880.1
- EnsemblBacteria:   EBMYCT00000014700
- GeneID:   1091354
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2231
- GeneTree:   EBGT00050000016841
- HOGENOM:   HBG729392
- OMA:   WADVQLA
- ProtClustDB:   PRK00235
- BioCyc:   MBOV233413:MB2231-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 24320; Mature: 24320

Theoretical pI: Translated: 11.48; Mature: 11.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16bc1c40)-; HASH(0x17139dd4)-; HASH(0x16fc3664)-; HASH(0x17127784)-; HASH(0x164216b4)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSP
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCC
LSGMLTVAVLLVVTRGLHIDGVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
LQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRSVPAAHGSTLGSRVAGTQPAPVVAAW
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEHHCCCCCCCCHHHHHCCCCCCHHHHHHH
LAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VTLAGLARL
HHHHHHHCH
>Mature Secondary Structure
MMRSLATAFAFATVIPTPGSATTPMGRGPMTALPVVGAALGALAAAIAWAGAQVFGPSSP
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCC
LSGMLTVAVLLVVTRGLHIDGVADTADGLGCYGPPQRALAVMRDGSTGPFGVAAVVLVIA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
LQGLAFATLTTVGIAGITLAVLSGRVTAVLVCRRSVPAAHGSTLGSRVAGTQPAPVVAAW
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEHHCCCCCCCCHHHHHCCCCCCHHHHHHH
LAVLLAVSVPAGPRPWQGPIAVLVAVTAGAALAAHCVHRFGGVTGDVLGSAIELSTTVSA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VTLAGLARL
HHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972