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The map label for this gene is pimB

Identifier: 121638069

GI number: 121638069

Start: 2428523

End: 2429680

Strand: Reverse

Name: pimB

Synonym: BCG_2204c

Alternate gene names: 121638069

Gene position: 2429680-2428523 (Counterclockwise)

Preceding gene: 121638070

Following gene: 121638066

Centisome position: 55.54

GC content: 68.31

Gene sequence:

>1158_bases
GTGAGCCGGGTCCTGTTGGTAACTAACGACTTTCCACCCCGACGCGGGGGCATCCAGTCCTATCTGGGGGAGTTCGTCGG
TCGGCTGGTCGGCTCCCGAGCGCATGCGATGACAGTGTATGCACCGCAGTGGAAAGGCGCCGACGCCTTCGACGACGCAG
CCCGTGCTGCCGGTTACCGGGTGGTGCGCCATCCCAGCACGGTGATGCTGCCGGGTCCAACGGTCGATGTCCGGATGCGC
CGGCTGATCGCCGAGCACGACATCGAAACCGTCTGGTTCGGTGCGGCCGCACCGCTGGCCCTGCTGGCCCCGCGTGCCCG
GCTGGCTGGAGCTAGCCGGGTGTTGGCCAGCACGCACGGCCACGAAGTGGGCTGGTCGATGCTTCCGGTCGCTCGATCGG
TGCTACGCCGCATCGGCGACGGCACCGACGTGGTGACTTTCGTCAGCAGCTACACGCGGTCCCGGTTTGCTTCTGCGTTC
GGGCCCGCGGCTTCGTTGGAATACCTACCGCCGGGAGTGGACACCGACCGGTTCCGCCCCGATCCGGCCGCACGCGCCGA
GTTGCGGAAGCGCTATCGGCTAGGCGAGCGGCCCACGGTAGTGTGTCTGTCGCGGTTGGTGCCGCGCAAAGGCCAGGACA
CCCTGGTCACGGCGCTCCCGTCGATCCGGCGTCGCGTTGATGGAGCCGCGTTGGTGATTGTCGGCGGCGGTCCATATCTA
GAGACGTTGCGCAAGCTGGCTCACGACTGTGGGGTGGCCGACCACGTGACGTTCACCGGCGGCGTGGCAACCGACGAACT
TCCCGCCCACCACGCACTGGCCGACGTGTTCGCCATGCCATGCCGCACCCGCGGCGCCGGGATGGACGTCGAGGGCTTGG
GCATCGTGTTCCTCGAAGCCTCCGCCGCCGGCGTGCCGGTAATCGCCGGCAATTCTGGCGGAGCGCCGGAAACGGTGCAG
CACAACAAGACTGGGCTAGTGGTCGACGGTAGGTCGGTGGACCGGGTGGCCGACGCCGTGGCCGAGTTGCTGATCGATCG
GGATCGGGCCGTCGCGATGGGCGCCGCCGGCCGAGAATGGGTCACGGCGCAGTGGCGTTGGGACACGCTGGCCGCCAAGC
TAGCAGATTTTCTGCGCGGCGACGATGCGGCCCGTTGA

Upstream 100 bases:

>100_bases
CTAGCTTCCGCCGCGAGCAGACGCAAAATCACCCGGAAACCGGCGATTTTGGGCGACTTTGCGTCTGCTCACGCAAGGTG
GACCCGCTAGCCTGACCCAG

Downstream 100 bases:

>100_bases
GCGGGCCGGGGAGGAGCCGGGCACAGTCGGCTATTCCTTGTTGTAGATCGCCTCGATATCGGAAGCGAACTTCTCGGCCA
CCACCTTGCGTTTGACCTTC

Product: hypothetical protein

Products: NA

Alternate protein names: Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase; Alpha-mannosyltransferase; Alpha-manT; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSRVLLVTNDFPPRRGGIQSYLGEFVGRLVGSRAHAMTVYAPQWKGADAFDDAARAAGYRVVRHPSTVMLPGPTVDVRMR
RLIAEHDIETVWFGAAAPLALLAPRARLAGASRVLASTHGHEVGWSMLPVARSVLRRIGDGTDVVTFVSSYTRSRFASAF
GPAASLEYLPPGVDTDRFRPDPAARAELRKRYRLGERPTVVCLSRLVPRKGQDTLVTALPSIRRRVDGAALVIVGGGPYL
ETLRKLAHDCGVADHVTFTGGVATDELPAHHALADVFAMPCRTRGAGMDVEGLGIVFLEASAAGVPVIAGNSGGAPETVQ
HNKTGLVVDGRSVDRVADAVAELLIDRDRAVAMGAAGREWVTAQWRWDTLAAKLADFLRGDDAAR

Sequences:

>Translated_385_residues
MSRVLLVTNDFPPRRGGIQSYLGEFVGRLVGSRAHAMTVYAPQWKGADAFDDAARAAGYRVVRHPSTVMLPGPTVDVRMR
RLIAEHDIETVWFGAAAPLALLAPRARLAGASRVLASTHGHEVGWSMLPVARSVLRRIGDGTDVVTFVSSYTRSRFASAF
GPAASLEYLPPGVDTDRFRPDPAARAELRKRYRLGERPTVVCLSRLVPRKGQDTLVTALPSIRRRVDGAALVIVGGGPYL
ETLRKLAHDCGVADHVTFTGGVATDELPAHHALADVFAMPCRTRGAGMDVEGLGIVFLEASAAGVPVIAGNSGGAPETVQ
HNKTGLVVDGRSVDRVADAVAELLIDRDRAVAMGAAGREWVTAQWRWDTLAAKLADFLRGDDAAR
>Mature_384_residues
SRVLLVTNDFPPRRGGIQSYLGEFVGRLVGSRAHAMTVYAPQWKGADAFDDAARAAGYRVVRHPSTVMLPGPTVDVRMRR
LIAEHDIETVWFGAAAPLALLAPRARLAGASRVLASTHGHEVGWSMLPVARSVLRRIGDGTDVVTFVSSYTRSRFASAFG
PAASLEYLPPGVDTDRFRPDPAARAELRKRYRLGERPTVVCLSRLVPRKGQDTLVTALPSIRRRVDGAALVIVGGGPYLE
TLRKLAHDCGVADHVTFTGGVATDELPAHHALADVFAMPCRTRGAGMDVEGLGIVFLEASAAGVPVIAGNSGGAPETVQH
NKTGLVVDGRSVDRVADAVAELLIDRDRAVAMGAAGREWVTAQWRWDTLAAKLADFLRGDDAAR

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose resid

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

Organism=Escherichia coli, GI1788357, Length=211, Percent_Identity=28.436018957346, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32564184, Length=248, Percent_Identity=23.7903225806452, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMB_MYCTU (O53522)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842579
- PIR:   F70937
- RefSeq:   NP_216704.2
- RefSeq:   NP_336715.1
- ProteinModelPortal:   O53522
- SMR:   O53522
- EnsemblBacteria:   EBMYCT00000002623
- EnsemblBacteria:   EBMYCT00000069686
- GeneID:   887278
- GeneID:   924183
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2243
- KEGG:   mtu:Rv2188c
- TIGR:   MT2243
- TubercuList:   Rv2188c
- GeneTree:   EBGT00050000015521
- HOGENOM:   HBG726846
- OMA:   NDFPPRP
- ProtClustDB:   CLSK799370
- GO:   GO:0040007
- GO:   GO:0009405
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: =2.4.1.57

Molecular weight: Translated: 41221; Mature: 41089

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: BINDING 17-17 BINDING 205-205 BINDING 262-262 BINDING 266-266 BINDING 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVLLVTNDFPPRRGGIQSYLGEFVGRLVGSRAHAMTVYAPQWKGADAFDDAARAAGYR
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCE
VVRHPSTVMLPGPTVDVRMRRLIAEHDIETVWFGAAAPLALLAPRARLAGASRVLASTHG
EEECCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHCCC
HEVGWSMLPVARSVLRRIGDGTDVVTFVSSYTRSRFASAFGPAASLEYLPPGVDTDRFRP
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DPAARAELRKRYRLGERPTVVCLSRLVPRKGQDTLVTALPSIRRRVDGAALVIVGGGPYL
CHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHH
ETLRKLAHDCGVADHVTFTGGVATDELPAHHALADVFAMPCRTRGAGMDVEGLGIVFLEA
HHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
SAAGVPVIAGNSGGAPETVQHNKTGLVVDGRSVDRVADAVAELLIDRDRAVAMGAAGREW
CCCCCEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCCCE
VTAQWRWDTLAAKLADFLRGDDAAR
EEEEECHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRVLLVTNDFPPRRGGIQSYLGEFVGRLVGSRAHAMTVYAPQWKGADAFDDAARAAGYR
CEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCE
VVRHPSTVMLPGPTVDVRMRRLIAEHDIETVWFGAAAPLALLAPRARLAGASRVLASTHG
EEECCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHCCC
HEVGWSMLPVARSVLRRIGDGTDVVTFVSSYTRSRFASAFGPAASLEYLPPGVDTDRFRP
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DPAARAELRKRYRLGERPTVVCLSRLVPRKGQDTLVTALPSIRRRVDGAALVIVGGGPYL
CHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHH
ETLRKLAHDCGVADHVTFTGGVATDELPAHHALADVFAMPCRTRGAGMDVEGLGIVFLEA
HHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
SAAGVPVIAGNSGGAPETVQHNKTGLVVDGRSVDRVADAVAELLIDRDRAVAMGAAGREW
CCCCCEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCCCE
VTAQWRWDTLAAKLADFLRGDDAAR
EEEEECHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036