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The map label for this gene is pncA [H]

Identifier: 121637929

GI number: 121637929

Start: 2272333

End: 2272893

Strand: Reverse

Name: pncA [H]

Synonym: BCG_2062c

Alternate gene names: 121637929

Gene position: 2272893-2272333 (Counterclockwise)

Preceding gene: 121637930

Following gene: 121637928

Centisome position: 51.96

GC content: 64.71

Gene sequence:

>561_bases
ATGCGGGCGTTGATCATCGTCGACGTGCAGAACGACTTCTGCGAGGGTGGCTCGCTGGCGGTAACCGGTGGCGCCGCGCT
GGCCCGCGCCATCAGCGACTACCTGGCCGAAGCGGCGGACTACCATCACGTCGTGGCAACCAAGGACTTCCACATCGACC
CGGGTGACGACTTCTCCGGCACACCGGACTATTCCTCGTCGTGGCCACCGCATTGCGTCAGCGGTACTCCCGGCGCGGAC
TTCCATCCCAGTCTGGACACGTCGGCAATCGAGGCGGTGTTCTACAAGGGTGCCTACACCGGAGCGTACAGCGGCTTCGA
AGGAGTCGACGAGAACGGCACGCCACTGCTGAATTGGCTGCGGCAACGCGGCGTCGATGAGGTCGATGTGGTCGGTATTG
CCACCGATCATTGTGTGCGCCAGACGGCCGAGGACGCGGTACGCAATGGCTTGGCCACCAGGGTGCTGGTGGACCTGACA
GCGGGTGTGTCGGCCGATACCACCGTCGCCGCGCTGGAGGAGATGCGCACCGCCAGCGTCGAGTTGGTTTGCAGCTCCTG
A

Upstream 100 bases:

>100_bases
GTCATGTTCGCGATCGTCGCGGCGTCATGGACCCTATATCTGTGGCTGCCGCGTCGGTAGGCAAACTGCCCGGGCAGTCG
CCCGAACGTATGGTGGACGT

Downstream 100 bases:

>100_bases
TGGCACCGCCGAACCGGGATGAACTGTTGGCGGCGGTGGAGCGCTCGCCGCAAGCGGCCGCCGCGCACGACCGCGCCGGC
TGGGTCGGGTTGTTCACCGG

Product: pyrazinamidase/nicotinamidas pncA

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MRALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHHVVATKDFHIDPGDDFSGTPDYSSSWPPHCVSGTPGAD
FHPSLDTSAIEAVFYKGAYTGAYSGFEGVDENGTPLLNWLRQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLT
AGVSADTTVAALEEMRTASVELVCSS

Sequences:

>Translated_186_residues
MRALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHHVVATKDFHIDPGDDFSGTPDYSSSWPPHCVSGTPGAD
FHPSLDTSAIEAVFYKGAYTGAYSGFEGVDENGTPLLNWLRQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLT
AGVSADTTVAALEEMRTASVELVCSS
>Mature_186_residues
MRALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHHVVATKDFHIDPGDDFSGTPDYSSSWPPHCVSGTPGAD
FHPSLDTSAIEAVFYKGAYTGAYSGFEGVDENGTPLLNWLRQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLT
AGVSADTTVAALEEMRTASVELVCSS

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=204, Percent_Identity=35.7843137254902, Blast_Score=104, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321401, Length=214, Percent_Identity=30.8411214953271, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 19583; Mature: 19583

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHHVVATKDFHIDPGDDFSG
CCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCC
TPDYSSSWPPHCVSGTPGADFHPSLDTSAIEAVFYKGAYTGAYSGFEGVDENGTPLLNWL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
RQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLTAGVSADTTVAALEEMRTASV
HHCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHEEHHHCCCCCCHHHHHHHHHHHCCE
ELVCSS
EEEECC
>Mature Secondary Structure
MRALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHHVVATKDFHIDPGDDFSG
CCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCC
TPDYSSSWPPHCVSGTPGADFHPSLDTSAIEAVFYKGAYTGAYSGFEGVDENGTPLLNWL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
RQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLTAGVSADTTVAALEEMRTASV
HHCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHEEHHHCCCCCCHHHHHHHHHHHCCE
ELVCSS
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]