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The map label for this gene is fadD31 [H]

Identifier: 121637830

GI number: 121637830

Start: 2179247

End: 2181109

Strand: Direct

Name: fadD31 [H]

Synonym: BCG_1964

Alternate gene names: 121637830

Gene position: 2179247-2181109 (Clockwise)

Preceding gene: 121637828

Following gene: 121637832

Centisome position: 49.82

GC content: 64.9

Gene sequence:

>1863_bases
ATGAATGACGGCTCCCGGCAGGAACTCAGGGTTCGTAGCGGCCTACTACAAATCGAGGACTGCCTGGATGCTGACGGCGG
CATCGCATTGCCGGCAGGCACCACGCTGATCTCGCTCATCGAGCGCAACATCAAGTATGTCGGCGACCTCGTGGCGTATC
GCTACCTGGACCACGCCCGTTCGGCCGCCGGATGCGCCCTGGAAGTGACCTGGACGCAATTCGGTATGCGATTAGCGGCC
ATAGGTGCACACGTGCAACGGTTCGCAGGCCCCGGCGACCGCGTTGCGATCCTCGCACCACAGGGCATCGACTATGTTTG
CGGGTTCTACGCTGCAATCAAGGCAGGCACCGTCGCGGTGCCGTTGTTCGCACCCGAACTGCCGGGTCACGCCGAGCGTC
TTGATACGGCACTTCGCGATTCGGAGCCAGCGGTCATACTCACGACGGCGGCGGCGAAAAACGCCGTTGAAGGTTTTCTG
AACAACGTTCCGCGCCTGCGAAAGCCGACAGTCCTCGTCATCGATCAAATACCCGACCGCGAGGGGGAGCTGTTCGTCCC
GGTCGAGCTGGACATCGACGCCGTATCCCACCTGCAGTACACCTCGGGCTCGACGCGACCCCCGGTCGGTGTCGAGATCA
CCCACCGCGCGGTCGGCACCAACCTGGTGCAAATGATCCTGTCGATCGACCTGCTCAACCGAAACACCCACGGCGTCAGT
TGGTTACCGCTGTACCACGACATGGGCCTATCCATGATCGGCTTTCCGGCGGTCTATGGCGGACACTCCACCCTGATGTC
GCCCACGGCGTTTGTCCGCAGGCCACTGCGATGGATCCAGGCGTTGTCCGAGGGGTCGCGGACCGGACGCGTGGTCACCG
CGGCGCCAAACTTCGCCTACGAGTGGGCCGCACAGCGTGGACTACCCGCGCAAGGCGACGACGTCGACCTCAGCAATGTC
GTGCTGATCATCGGTTCCGAACCAGTCAGCATCGATGCGGTGACCACGTTCAACAAAGCGTTCGCGCCCTATGGTTTACC
GCGTACAGCGTTCAAACCCTCGTACGGCATAGCCGAGGCGACCCTGCTCGTCGCGACCATCGACCATGCCGCTGAGCCGA
CGGTTGTTTATCTTGACCCAGAGCAGTTGGGCGCCGGACACGCGACGCGCGTCGCGCCGGATGCGCCCAACGCCGTCGTG
CACGTGTCGTGTGGCCATGTGGCCCGCAGCCTGTGGGCCGTGATCGTCGACCCGGATACCGGCCCCGAGGCGGGCGCCGA
ACTGCCCGACGGTGAGATCGGTGAGGTTTGGTTACAAGGCGACAACGTTGCTCGGGGGTATTGGGGACGGCCGGAAGAAA
CGCGGATGACGTTCGGTGCCCGCTTGCAATCACCGCTCGCCGAAGGCAGCCACGCCGACGGGTCCGCGATCGACGACACC
TGGCTGCGCACCGGAGACCTCGGCGTGTACCTCGACGGTGAGCTCTACATCACCGGTCGAATCGCGGATCTGCTGACCAT
CGACGGCCGCAACCACTATCCGCAGGACATCGAGGCCACGGCCGCCGAGGCCTCGCCGATGGTGCGGCGCGGATACATAA
CCGCTTTCACGGTGCCGGCCAGCGACGGGGACGACCGCAATCAGCGACTGGTGATCATCGCCGAACGTGCGGCAGGCACC
AGTCGCAGCGACCCGCGGCCGGCGCTCGACGCGATTCGCGCAGCGGTTTGCAACCGCCACGGGTTATCCGTTGCGGACCT
GAGTTTCCTGCCGGCCGGCGCCATTCCACGCACCACCAGCGGGAAGCTGGCTCGCCAGGCCTGCCGCGCCCAATACCTCA
GCGGTCGCCTGGGCGTGCATTAG

Upstream 100 bases:

>100_bases
TTGGTTCGGACTCTAGTGGAGCCCGGTTGCGCACTCGGGTCCGACCAATGCGGGGCCGCGCCTCATACGCACGATAAGCG
TGGGTGCATAGACTGCGGTT

Downstream 100 bases:

>100_bases
CTACGATCTACGGCTCCCAAATCAGCAGATCCTCCATGCCGTTGTTCATCGCGACGATGGTTGGCGATGGGCCGGTGACA
TCGAAGTAGATTTTGCCGGT

Product: putative fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MNDGSRQELRVRSGLLQIEDCLDADGGIALPAGTTLISLIERNIKYVGDLVAYRYLDHARSAAGCALEVTWTQFGMRLAA
IGAHVQRFAGPGDRVAILAPQGIDYVCGFYAAIKAGTVAVPLFAPELPGHAERLDTALRDSEPAVILTTAAAKNAVEGFL
NNVPRLRKPTVLVIDQIPDREGELFVPVELDIDAVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLNRNTHGVS
WLPLYHDMGLSMIGFPAVYGGHSTLMSPTAFVRRPLRWIQALSEGSRTGRVVTAAPNFAYEWAAQRGLPAQGDDVDLSNV
VLIIGSEPVSIDAVTTFNKAFAPYGLPRTAFKPSYGIAEATLLVATIDHAAEPTVVYLDPEQLGAGHATRVAPDAPNAVV
HVSCGHVARSLWAVIVDPDTGPEAGAELPDGEIGEVWLQGDNVARGYWGRPEETRMTFGARLQSPLAEGSHADGSAIDDT
WLRTGDLGVYLDGELYITGRIADLLTIDGRNHYPQDIEATAAEASPMVRRGYITAFTVPASDGDDRNQRLVIIAERAAGT
SRSDPRPALDAIRAAVCNRHGLSVADLSFLPAGAIPRTTSGKLARQACRAQYLSGRLGVH

Sequences:

>Translated_620_residues
MNDGSRQELRVRSGLLQIEDCLDADGGIALPAGTTLISLIERNIKYVGDLVAYRYLDHARSAAGCALEVTWTQFGMRLAA
IGAHVQRFAGPGDRVAILAPQGIDYVCGFYAAIKAGTVAVPLFAPELPGHAERLDTALRDSEPAVILTTAAAKNAVEGFL
NNVPRLRKPTVLVIDQIPDREGELFVPVELDIDAVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLNRNTHGVS
WLPLYHDMGLSMIGFPAVYGGHSTLMSPTAFVRRPLRWIQALSEGSRTGRVVTAAPNFAYEWAAQRGLPAQGDDVDLSNV
VLIIGSEPVSIDAVTTFNKAFAPYGLPRTAFKPSYGIAEATLLVATIDHAAEPTVVYLDPEQLGAGHATRVAPDAPNAVV
HVSCGHVARSLWAVIVDPDTGPEAGAELPDGEIGEVWLQGDNVARGYWGRPEETRMTFGARLQSPLAEGSHADGSAIDDT
WLRTGDLGVYLDGELYITGRIADLLTIDGRNHYPQDIEATAAEASPMVRRGYITAFTVPASDGDDRNQRLVIIAERAAGT
SRSDPRPALDAIRAAVCNRHGLSVADLSFLPAGAIPRTTSGKLARQACRAQYLSGRLGVH
>Mature_620_residues
MNDGSRQELRVRSGLLQIEDCLDADGGIALPAGTTLISLIERNIKYVGDLVAYRYLDHARSAAGCALEVTWTQFGMRLAA
IGAHVQRFAGPGDRVAILAPQGIDYVCGFYAAIKAGTVAVPLFAPELPGHAERLDTALRDSEPAVILTTAAAKNAVEGFL
NNVPRLRKPTVLVIDQIPDREGELFVPVELDIDAVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLNRNTHGVS
WLPLYHDMGLSMIGFPAVYGGHSTLMSPTAFVRRPLRWIQALSEGSRTGRVVTAAPNFAYEWAAQRGLPAQGDDVDLSNV
VLIIGSEPVSIDAVTTFNKAFAPYGLPRTAFKPSYGIAEATLLVATIDHAAEPTVVYLDPEQLGAGHATRVAPDAPNAVV
HVSCGHVARSLWAVIVDPDTGPEAGAELPDGEIGEVWLQGDNVARGYWGRPEETRMTFGARLQSPLAEGSHADGSAIDDT
WLRTGDLGVYLDGELYITGRIADLLTIDGRNHYPQDIEATAAEASPMVRRGYITAFTVPASDGDDRNQRLVIIAERAAGT
SRSDPRPALDAIRAAVCNRHGLSVADLSFLPAGAIPRTTSGKLARQACRAQYLSGRLGVH

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI225735629, Length=470, Percent_Identity=24.8936170212766, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI45827692, Length=470, Percent_Identity=24.8936170212766, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI44888818, Length=483, Percent_Identity=23.8095238095238, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI55749758, Length=467, Percent_Identity=22.6980728051392, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI225735625, Length=550, Percent_Identity=24.1818181818182, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193202679, Length=568, Percent_Identity=21.4788732394366, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24654656, Length=468, Percent_Identity=23.9316239316239, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 66311; Mature: 66311

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDGSRQELRVRSGLLQIEDCLDADGGIALPAGTTLISLIERNIKYVGDLVAYRYLDHAR
CCCCCHHHHHHHHCCHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SAAGCALEVTWTQFGMRLAAIGAHVQRFAGPGDRVAILAPQGIDYVCGFYAAIKAGTVAV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
PLFAPELPGHAERLDTALRDSEPAVILTTAAAKNAVEGFLNNVPRLRKPTVLVIDQIPDR
EEECCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
EGELFVPVELDIDAVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLNRNTHGVS
CCCEEEEEEECCHHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEE
WLPLYHDMGLSMIGFPAVYGGHSTLMSPTAFVRRPLRWIQALSEGSRTGRVVTAAPNFAY
EEEHHHHCCCCCCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH
EWAAQRGLPAQGDDVDLSNVVLIIGSEPVSIDAVTTFNKAFAPYGLPRTAFKPSYGIAEA
HHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHH
TLLVATIDHAAEPTVVYLDPEQLGAGHATRVAPDAPNAVVHVSCGHVARSLWAVIVDPDT
EEEEEECCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCEEEEEECCCC
GPEAGAELPDGEIGEVWLQGDNVARGYWGRPEETRMTFGARLQSPLAEGSHADGSAIDDT
CCCCCCCCCCCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCH
WLRTGDLGVYLDGELYITGRIADLLTIDGRNHYPQDIEATAAEASPMVRRGYITAFTVPA
HEEECCEEEEECCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCHHHCCEEEEEEEEC
SDGDDRNQRLVIIAERAAGTSRSDPRPALDAIRAAVCNRHGLSVADLSFLPAGAIPRTTS
CCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEHHCCCCCCCCCCCCC
GKLARQACRAQYLSGRLGVH
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNDGSRQELRVRSGLLQIEDCLDADGGIALPAGTTLISLIERNIKYVGDLVAYRYLDHAR
CCCCCHHHHHHHHCCHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SAAGCALEVTWTQFGMRLAAIGAHVQRFAGPGDRVAILAPQGIDYVCGFYAAIKAGTVAV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
PLFAPELPGHAERLDTALRDSEPAVILTTAAAKNAVEGFLNNVPRLRKPTVLVIDQIPDR
EEECCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
EGELFVPVELDIDAVSHLQYTSGSTRPPVGVEITHRAVGTNLVQMILSIDLLNRNTHGVS
CCCEEEEEEECCHHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEE
WLPLYHDMGLSMIGFPAVYGGHSTLMSPTAFVRRPLRWIQALSEGSRTGRVVTAAPNFAY
EEEHHHHCCCCCCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH
EWAAQRGLPAQGDDVDLSNVVLIIGSEPVSIDAVTTFNKAFAPYGLPRTAFKPSYGIAEA
HHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHH
TLLVATIDHAAEPTVVYLDPEQLGAGHATRVAPDAPNAVVHVSCGHVARSLWAVIVDPDT
EEEEEECCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCEEEEEECCCC
GPEAGAELPDGEIGEVWLQGDNVARGYWGRPEETRMTFGARLQSPLAEGSHADGSAIDDT
CCCCCCCCCCCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCH
WLRTGDLGVYLDGELYITGRIADLLTIDGRNHYPQDIEATAAEASPMVRRGYITAFTVPA
HEEECCEEEEECCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCHHHCCEEEEEEEEC
SDGDDRNQRLVIIAERAAGTSRSDPRPALDAIRAAVCNRHGLSVADLSFLPAGAIPRTTS
CCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEHHCCCCCCCCCCCCC
GKLARQACRAQYLSGRLGVH
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]