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The map label for this gene is rffA [H]

Identifier: 121637436

GI number: 121637436

Start: 1719069

End: 1720217

Strand: Reverse

Name: rffA [H]

Synonym: BCG_1567c

Alternate gene names: 121637436

Gene position: 1720217-1719069 (Counterclockwise)

Preceding gene: 121637437

Following gene: 121637431

Centisome position: 39.32

GC content: 60.14

Gene sequence:

>1149_bases
GTGTCAGATCACAAAGTTCCATTCAATCGGCCGTACATGACCGGCCGGGAGCTCGCGTACATCGCCGAAGCGCATTCTTG
CGGCCACCTGGCCGGCGATGGTCCCTTCACCCGGCGTAGCCATGCATGGCTCGAACAGCAGACCGGTTGTCGCAAGGCAC
TACTCACCCCCTCATGCACCGCCGCGCTGGAAATGATGGCGCTGCTTCTGGACATAGAGGAGGGGGACGAAGTCATACTG
CCGTCATACACCTTTGTCTCTACCGCCAACGCCTTCGTGTTGCGCGGTGGTGTGCCAGTCTTTGTCGATATTCGGCCCGA
CACGCTCAACATTGATGAAACTCGCATCGTAGACGCCATCACCCCGCGAACCAAGGCCATCGTCCCCGTTCACTATGCCG
GCGTGGCCTGCGAGATGGACGCGATCATGAAGATCGCCACGCACCACAACCTGGCGGTGGTCGAAGACGCGGCCCAAGGC
GCGATGGCGTCGTATCGTGGGCGGGCGCTCGGCAGCATCGGCGACCTGGGAGCGCTCTCATTTCACGAGACCAAGAATGT
GATTTCCGGCGAAGGCGGCGCCCTGCTTGTCAACTCAGAAGACTTCCTGCTCCGGGCAGAGATTCTCAGGGAAAAGGGCA
CCAATCGCAGCCGCTTCCTTCGCAACGAGGTCGACAAATATACGTGGCAGGACAAAGGGTCTTCCTATTTGCCCAGCGAA
TTAGTCGCTGCCTTTCTATGGGCTCAGTTCGAGGAAGCCGAGCGGATCACGCGTATCCGATTGGACCTATGGAACCGGTA
TCATGAAAGCTTCGAATCATTGGAACAGCGGGGGCTCCTGCGCCGTCCGATCATCCCACAGGGCTGCTCTCACAACGCCC
ACATGTACTACGTGTTACTAGCGCCCAGCGCCGATCGGGAGGAGGTGCTGGCGCGTCTGACGAGCAAAGGTATAGGCGCG
GTCTTTCATTACGTGCCGCTTCACGATTCGCCGGCCGGGCGTCGCTACGGCCGCACCAATGGCAACCTGACCGTCACCAA
CGACGTGGCTTCCCGCCTAATCCGTCTGCCGATGTGGGTTGGACTTCAGGAGGTAGATCAGAGCCGCGTTGTCGAGGCGC
TCACGCGCATCCTGACGTTGCGCGCATGA

Upstream 100 bases:

>100_bases
CTAGGGATCCGCCGGCCACAAGGAGAATTCTGGTGGATGTTCGACCCCGATCACCGAACACTTCAGCGCCCTGTTCGATG
ATTTGAGAAGTTGGCGTCGA

Downstream 100 bases:

>100_bases
AGATAAGCAGTGCGAAGGATACAATGAAGCCCTTAAAGGGCCTCGACGAGCAGTGAGTGATCGCGGCGACGACGATAATC
CGGGAAATCATCGCTACCGC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCTAALEMMALLLDIEEGDEVIL
PSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAITPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQG
AMASYRGRALGSIGDLGALSFHETKNVISGEGGALLVNSEDFLLRAEILREKGTNRSRFLRNEVDKYTWQDKGSSYLPSE
LVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLLAPSADREEVLARLTSKGIGA
VFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWVGLQEVDQSRVVEALTRILTLRA

Sequences:

>Translated_382_residues
MSDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCTAALEMMALLLDIEEGDEVIL
PSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAITPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQG
AMASYRGRALGSIGDLGALSFHETKNVISGEGGALLVNSEDFLLRAEILREKGTNRSRFLRNEVDKYTWQDKGSSYLPSE
LVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLLAPSADREEVLARLTSKGIGA
VFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWVGLQEVDQSRVVEALTRILTLRA
>Mature_381_residues
SDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCTAALEMMALLLDIEEGDEVILP
SYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAITPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQGA
MASYRGRALGSIGDLGALSFHETKNVISGEGGALLVNSEDFLLRAEILREKGTNRSRFLRNEVDKYTWQDKGSSYLPSEL
VAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLLAPSADREEVLARLTSKGIGAV
FHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWVGLQEVDQSRVVEALTRILTLRA

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=370, Percent_Identity=58.3783783783784, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI145693159, Length=385, Percent_Identity=30.6493506493506, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42556; Mature: 42425

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCT
CCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCHH
AALEMMALLLDIEEGDEVILPSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAI
HHHHHHHHHHHHCCCCCEEECCCHHHHCCCEEEEECCCEEEEEECCCCCCCCHHHEEECC
TPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQGAMASYRGRALGSIGDLGALS
CCCCCEEEEEEECCEEEHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
FHETKNVISGEGGALLVNSEDFLLRAEILREKGTNRSRFLRNEVDKYTWQDKGSSYLPSE
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCEECCCCCCCCCHH
LVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEE
APSADREEVLARLTSKGIGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWV
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHC
GLQEVDQSRVVEALTRILTLRA
CHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCT
CCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCHH
AALEMMALLLDIEEGDEVILPSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAI
HHHHHHHHHHHHCCCCCEEECCCHHHHCCCEEEEECCCEEEEEECCCCCCCCHHHEEECC
TPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQGAMASYRGRALGSIGDLGALS
CCCCCEEEEEEECCEEEHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
FHETKNVISGEGGALLVNSEDFLLRAEILREKGTNRSRFLRNEVDKYTWQDKGSSYLPSE
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCEECCCCCCCCCHH
LVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEE
APSADREEVLARLTSKGIGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWV
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHC
GLQEVDQSRVVEALTRILTLRA
CHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]