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The map label for this gene is 121637355

Identifier: 121637355

GI number: 121637355

Start: 1624446

End: 1625825

Strand: Direct

Name: 121637355

Synonym: BCG_1486

Alternate gene names: NA

Gene position: 1624446-1625825 (Clockwise)

Preceding gene: 121637353

Following gene: 121637359

Centisome position: 37.13

GC content: 65.29

Gene sequence:

>1380_bases
ATGAAGCGGCTTTCGAGCGTTGATGCTGCGTTTTGGTCCGCGGAAACCGCAGGCTGGCATATGCACGTGGGCGCACTGGC
GATCTGCGATCCCAGCGACGCGCCCGAATACAGCTTTCAGCGGCTCCGCGAGTTGATCATCGAACGGCTGCCGGAGATCC
CGCAGTTGCGGTGGCGGGTCACCGGCGCCCCGCTCGGACTGGACCGGCCGTGGTTCGTCGAGGACGAGGAACTCGACATC
GACTTTCACATCCGCCGCATCGGTGTTCCGGCTCCCGGTGGGCGGCGCGAACTCGAGGAGCTCGTCGGACGGCTGATGTC
CTACAAACTGGACCGTTCCCGGCCGCTGTGGGAACTGTGGGTCATCGAGGGCGTCGAGGGCGGCCGCATCGCCACGCTGA
CCAAGATGCATCACGCCATCGTCGACGGTGTCTCCGGTGCCGGGCTGGGCGAAATCCTGTTGGACATCACACCAGAACCA
CGACCACCGCAACAGGAAACGGTCGGCTTCGTGGGATTCCAGATTCCGGGCCTGGAACGCCGGGCGATAGGTGCGCTGAT
CAACGTGGGCATCATGACGCCCTTCCGCATCGTCAGGCTGCTGGAGCAAACCGTGCGTCAACAGATCGCGGCATTGGGTG
TGGCCGGCAAACCGGCGCGATACTTCGAAGCGCCCAAGACGCGGTTCAATGCGCCGGTGTCGCCGCACCGGCGGGTTACC
GGCACACGCGTCGAGCTGGCTAGGGCCAAAGCGGTCAAGGACGCGTTCGGCGTCAAGCTCAACGACGTCGTCTTGGCGCT
GGTGGCCGGGGCGGCCCGGCAATACCTACAGAAGCGTGACGAGCTGCCCGCCAAGCCGTTGATCGCGCAGATTCCGGTCT
CCACCCGCAGCGAGGAAACGAAGGCCGACGTCGGGAACCAGGTCAGCTCGATGACCGCGTCGCTGGCAACCCATATCGAG
GATCCGGCCAAGCGCCTGGCGGCCATCCACGAGAGCACCCTCAGCGCCAAGGAAATGGCTAAGGCGCTCTCCGCGCACCA
GATCATGGGGCTGACCGAGACCACGCCACCGGGTCTGCTGCAGCTGGCCGCCCGGGCCTATACGGCCAGCGGGCTGTCAC
ACAACCTGGCCCCAATCAACCTCGTCGTCTCCAATGTCCCCGGTCCACCCTTCCCGCTATATATGGCCGGCGCGCGGCTG
GATTCGCTGGTGCCCCTGGGGCCGCCGGTGATGGACGTGGCGCTGAACATCACCTGCTTCTCCTACCAGGATTATCTGGA
TTTCGGCCTGGTGACCACACCCGAGGTGGCCAACGACATCGACGAGATGGCCGATGCCATCGAACCGGCACTGGCCGAGC
TGGAGCGTGCCGCGGAATAG

Upstream 100 bases:

>100_bases
TCGCCGATGTTTGGGCCGAAGCTTGCACACATTGACGATAACTTGGTCGCGAGACCGCAGAAGCTGGCCTCGACGGCGCG
CCGGGGACTACGGTCATACC

Downstream 100 bases:

>100_bases
CAATAGCTGGCCTATAGCTGACTACGTGGCCGGCGGGTTGGTCGCGTACACCCAAGACAGGAAGCGGGCCACGGCCTCGG
CGGTGTGATGCGCCCGCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468 [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRVTGAPLGLDRPWFVEDEELDI
DFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELWVIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEP
RPPQQETVGFVGFQIPGLERRAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEETKADVGNQVSSMTASLATHIE
DPAKRLAAIHESTLSAKEMAKALSAHQIMGLTETTPPGLLQLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARL
DSLVPLGPPVMDVALNITCFSYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE

Sequences:

>Translated_459_residues
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRVTGAPLGLDRPWFVEDEELDI
DFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELWVIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEP
RPPQQETVGFVGFQIPGLERRAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEETKADVGNQVSSMTASLATHIE
DPAKRLAAIHESTLSAKEMAKALSAHQIMGLTETTPPGLLQLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARL
DSLVPLGPPVMDVALNITCFSYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE
>Mature_459_residues
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRVTGAPLGLDRPWFVEDEELDI
DFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELWVIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEP
RPPQQETVGFVGFQIPGLERRAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEETKADVGNQVSSMTASLATHIE
DPAKRLAAIHESTLSAKEMAKALSAHQIMGLTETTPPGLLQLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARL
DSLVPLGPPVMDVALNITCFSYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 50079; Mature: 50079

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRV
CCCCHHHHHHHHCCCCCCEEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
TGAPLGLDRPWFVEDEELDIDFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELW
ECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
VIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEPRPPQQETVGFVGFQIPGLER
EEECCCCCEEHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCHHHHCEEEEEECCCCHH
RAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHCCCCCCCCCCCC
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEET
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHH
KADVGNQVSSMTASLATHIEDPAKRLAAIHESTLSAKEMAKALSAHQIMGLTETTPPGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHH
QLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARLDSLVPLGPPVMDVALNITCF
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCHHHHEEEEEEE
SYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE
EHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRV
CCCCHHHHHHHHCCCCCCEEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
TGAPLGLDRPWFVEDEELDIDFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELW
ECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
VIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEPRPPQQETVGFVGFQIPGLER
EEECCCCCEEHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCHHHHCEEEEEECCCCHH
RAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHCCCCCCCCCCCC
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEET
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHH
KADVGNQVSSMTASLATHIEDPAKRLAAIHESTLSAKEMAKALSAHQIMGLTETTPPGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHH
QLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARLDSLVPLGPPVMDVALNITCF
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCHHHHEEEEEEE
SYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE
EHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]