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The map label for this gene is glgB

Identifier: 121637257

GI number: 121637257

Start: 1516631

End: 1518826

Strand: Reverse

Name: glgB

Synonym: BCG_1388c

Alternate gene names: 121637257

Gene position: 1518826-1516631 (Counterclockwise)

Preceding gene: 121637258

Following gene: 121637256

Centisome position: 34.72

GC content: 64.07

Gene sequence:

>2196_bases
ATGAGTCGATCCGAGAAACTCACCGGGGAGCACCTTGCACCCGAGCCGGCCGAAATGGCGCGCTTGGTGGCGGGTACACA
TCACAACCCGCACGGCATCCTGGGCGCCCACGAATACGGCGACCATACCGTCATCCGAGCGTTCCGTCCGCATGCCGTCG
AGGTCGTCGCGCTCGTTGGTAAGGACCGGTTCTCGTTGCAGCACCTCGATTCTGGCCTGTTTGCCGTCGCATTGCCGTTC
GTCGACCTCATCGACTACCGCCTGCAGGTGACCTATGAAGGTTGCGAGCCACACACCGTGGCCGATGCGTACCGATTCCT
GCCCACCCTGGGCGAGGTCGACCTGCACCTGTTCGCCGAGGGCCGCCACGAACGGCTTTGGGAAGTCCTGGGTGCCCACC
CCCGCTCGTTTACCACGGCCGACGGTGTGGTGAGTGGCGTGTCGTTCGCCGTGTGGGCGCCCAACGCCAAGGGCGTCAGC
TTGATCGGCGAGTTCAACGGTTGGAATGGCCACGAAGCCCCCATGCGGGTGCTCGGCCCATCAGGGGTATGGGAATTGTT
CTGGCCCGACTTCCCTTGCGACGGTCTGTACAAGTTCCGCGTGCACGGCGCCGACGGCGTGGTTACCGATCGGGCCGACC
CGTTCGCGTTCGGCACCGAGGTGCCGCCGCAGACCGCATCGCGGGTGACGTCGAGTGACTACACCTGGGGTGACGACGAC
TGGATGGCTGGGCGTGCGCTGCGCAACCCGGTGAACGAGGCGATGAGCACCTACGAAGTCCATCTCGGTTCGTGGCGGCC
TGGACTCAGCTACCGCCAGCTTGCTCGTGAGTTGACGGATTACATTGTGGATCAAGGGTTTACCCATGTGGAGCTGTTGC
CCGTCGCCGAGCATCCATTCGCCGGATCATGGGGGTATCAGGTCACGTCCTACTATGCGCCGACATCACGATTCGGCACA
CCCGACGACTTCCGGGCGCTGGTCGACGCCCTGCACCAGGCCGGCATCGGCGTCATCGTGGATTGGGTCCCAGCGCACTT
CCCGAAGGACGCGTGGGCCCTGGGACGGTTCGACGGCACTCCGCTCTACGAACATTCCGATCCCAAACGCGGCGAGCAAC
TGGATTGGGGCACATACGTGTTCGACTTCGGCCGCCCGGAAGTGCGCAACTTTCTGGTAGCCAATGCGTTGTACTGGCTA
CAGGAGTTCCACATCGACGGCCTGCGGGTGGACGCGGTGGCCTCAATGCTCTATCTAGACTACTCGCGACCCGAGGGCGG
CTGGACCCCCAACGTCCACGGCGGCCGGGAGAACCTGGAAGCAGTGCAGTTCCTGCAGGAGATGAACGCCACGGCGCACA
AGGTCGCGCCGGGAATCGTCACCATCGCCGAGGAGTCCACGTCGTGGCCTGGGGTGACCCGCCCGACCAACATTGGCGGC
CTGGGCTTTTCGATGAAGTGGAACATGGGCTGGATGCACGACACGCTCGACTACGTCAGCCGAGATCCGGTGTACCGCAG
CTACCACCACCACGAGATGACGTTCTCGATGCTGTATGCGTTCAGCGAAAATTACGTGTTGCCGCTCAGTCATGACGAGG
TGGTGCACGGCAAAGGCACGCTGTGGGGGCGGATGCCGGGCAACAATCACGTCAAGGCCGCCGGCCTGCGTAGCCTGCTT
GCCTACCAATGGGCACACCCCGGCAAGCAATTGCTGTTCATGGGTCAGGAATTCGGCCAACGCGCCGAATGGTCCGAGCA
GCGCGGCCTGGACTGGTTCCAACTCGACGAAAACGGCTTCTCCAACGGGATTCAGCGGCTGGTGCGCGACATCAACGACA
TCTACCGATGCCACCCGGCGCTGTGGAGCTTAGACACCACCCCCGAAGGCTATTCTTGGATCGACGCCAACGACTCCGCC
AACAATGTGTTGAGCTTTATGCGCTACGGCAGCGACGGCTCGGTGCTGGCCTGCGTGTTCAATTTCGCAGGTGCCGAACA
CCGTGACTATCGACTCGGGCTGCCGCGCGCGGGCCGCTGGCGCGAGGTGCTCAATACCGACGCGACGATCTACCACGGCT
CAGGGATCGGCAACCTCGGCGGCGTGGACGCCACCGACGACCCCTGGCATGGCCGCCCGGCGTCCGCGGTGCTGGTGCTG
CCGCCCACTTCGGCGCTGTGGCTGACGCCCGCCTAG

Upstream 100 bases:

>100_bases
ATCCGCATCGACCCAGCACGGGCAGTCGCCCACATCATCAACATGCCAGCCGTGCCCTACGAGAGCCGAAACACGCTGCT
GCGCAGGAGGTGACGGGCTC

Downstream 100 bases:

>100_bases
CGCCTGCCATGAGCAGATTGCACATGCCTGATGAGCCGATTCGATGCACTCTGCACACTGCACTCCGAGATTGATCAGAA
AGGCTAAGCGATAGCGCTAC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYGDHTVIRAFRPHAVEVVALVGKDRFSLQHLDSGLFAVALPF
VDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAEGRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVS
LIGEFNGWNGHEAPMRVLGPSGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGT
PDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWL
QEFHIDGLRVDAVASMLYLDYSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTSWPGVTRPTNIGG
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGNNHVKAAGLRSLL
AYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSA
NNVLSFMRYGSDGSVLACVFNFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
PPTSALWLTPA

Sequences:

>Translated_731_residues
MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYGDHTVIRAFRPHAVEVVALVGKDRFSLQHLDSGLFAVALPF
VDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAEGRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVS
LIGEFNGWNGHEAPMRVLGPSGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGT
PDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWL
QEFHIDGLRVDAVASMLYLDYSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTSWPGVTRPTNIGG
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGNNHVKAAGLRSLL
AYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSA
NNVLSFMRYGSDGSVLACVFNFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
PPTSALWLTPA
>Mature_730_residues
SRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYGDHTVIRAFRPHAVEVVALVGKDRFSLQHLDSGLFAVALPFV
DLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAEGRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSL
IGEFNGWNGHEAPMRVLGPSGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDDW
MAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGTP
DDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQ
EFHIDGLRVDAVASMLYLDYSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTSWPGVTRPTNIGGL
GFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGNNHVKAAGLRSLLA
YQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSAN
NVLSFMRYGSDGSVLACVFNFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVLP
PTSALWLTPA

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=657, Percent_Identity=28.310502283105, Blast_Score=187, Evalue=3e-47,
Organism=Escherichia coli, GI1789839, Length=720, Percent_Identity=48.75, Blast_Score=664, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=635, Percent_Identity=26.1417322834646, Blast_Score=166, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32564391, Length=513, Percent_Identity=26.9005847953216, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=641, Percent_Identity=25.4290171606864, Blast_Score=163, Evalue=8e-41,
Organism=Drosophila melanogaster, GI28573410, Length=605, Percent_Identity=27.7685950413223, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_MYCBO (P59816)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855015.1
- ProteinModelPortal:   P59816
- SMR:   P59816
- EnsemblBacteria:   EBMYCT00000016483
- GeneID:   1090651
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1361c
- GeneTree:   EBGT00050000016657
- HOGENOM:   HBG287139
- OMA:   PPLATIM
- ProtClustDB:   PRK05402
- BioCyc:   MBOV233413:MB1361C-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 81672; Mature: 81541

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: ACT_SITE 306-306 ACT_SITE 341-341 ACT_SITE 346-346 ACT_SITE 409-409 ACT_SITE 411-411 ACT_SITE 464-464 ACT_SITE 531-531 ACT_SITE 532-532

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYGDHTVIRAFRPHAVEVVALVG
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECC
KDRFSLQHLDSGLFAVALPFVDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAE
CCCHHHHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEC
GRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSLIGEFNGWNGHEAPMRVLGP
CHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEEECC
SGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
CCCEEEECCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPF
HHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
AGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHEECCCCCC
PLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQEFHIDGLRVDAVASMLYLD
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHEE
YSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTSWPGVTRPTNIGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT
CCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCE
LWGRMPGNNHVKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGF
EEEECCCCCCEEHHHHHHHHHHHHCCCCHHEEEECHHHCCCCCCHHHHCCCEEEECCCHH
SNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSANNVLSFMRYGSDGSVLACVF
HHHHHHHHHHHHHHHHHCHHHEECCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEE
NFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
HHCCCCCCCEEECCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
PPTSALWLTPA
CCCCCEEEECC
>Mature Secondary Structure 
SRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYGDHTVIRAFRPHAVEVVALVG
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECC
KDRFSLQHLDSGLFAVALPFVDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAE
CCCHHHHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEC
GRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSLIGEFNGWNGHEAPMRVLGP
CHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEEECC
SGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
CCCEEEECCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPF
HHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
AGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHEECCCCCC
PLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQEFHIDGLRVDAVASMLYLD
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHEE
YSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTSWPGVTRPTNIGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT
CCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCE
LWGRMPGNNHVKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGF
EEEECCCCCCEEHHHHHHHHHHHHCCCCHHEEEECHHHCCCCCCHHHHCCCEEEECCCHH
SNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSANNVLSFMRYGSDGSVLACVF
HHHHHHHHHHHHHHHHHCHHHEECCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEE
NFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
HHCCCCCCCEEECCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
PPTSALWLTPA
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972