Click here to switch to the map view.

The map label for this gene is amiB2

Identifier: 121637191

GI number: 121637191

Start: 1437145

End: 1438533

Strand: Direct

Name: amiB2

Synonym: BCG_1322

Alternate gene names: 121637191

Gene position: 1437145-1438533 (Clockwise)

Preceding gene: 121637188

Following gene: 121637192

Centisome position: 32.85

GC content: 68.03

Gene sequence:

>1389_bases
GTGGACCCTACTGACCTGGCCTTCGCCGGTGCCGCGGCACAGGCGCGGATGCTGGCTGACGGTGCACTCACCGCGCCGAT
GCTGCTCGAGGTCTACCTGCAACGAATTGAGCGTCTGGACAGCCACCTGCGCGCCTACCGGGTGGTGCAGTTCGACCGGG
CGCGTGCGGAGGCCGAGGCCGCCCAGCAACGCCTCGACGCCGGTGAGCGGCTGCCGCTCCTGGGCGTGCCGATCGCCATC
AAAGATGATGTCGACATCGCCGGGGAGGTGACGACATACGGCAGCGCCGGGCACGGTCCGGCCGCGACGTCCGACGCAGA
GGTGGTTCGCCGGCTGCGCGCGGCAGGCGCTGTCATCATCGGCAAAACCAACGTGCCTGAGTTGATGATCATGCCCTTCA
CCGAGTCGCTGGCCTTCGGGGCCACCCGGAATCCGTGGTGCCTCAATCGAACCCCTGGCGGCAGCAGCGGCGGCAGCGCT
GCGGCGGTAGCGGCCGGGCTGGCGCCAGTGGCACTGGGATCCGATGGTGGCGGATCGATTCGTATCCCGTGTACCTGGTG
CGGTCTGTTTGGGCTGAAACCACAGCGCGATCGGATTTCCTTGGAGCCGCACGACGGGGCCTGGCAGGGGCTGAGCGTCA
ATGGCCCGATCGCGCGGTCGGTAATGGACGCGGCGTTGCTACTGGACGCGACCACAACGGTGCCTGGTCCCGAAGGCGAG
TTTGTGGCCGCGGCCGCACGCCAACCCGGCCGGCTGCGAATTGCCTTGAGCACCAGGGTGCCAACCCCGCTGCCCGTTAG
GTGCGGCAAGCAAGAACTGGCAGCCGTCCACCAGGCAGGTGCGTTGCTACGTGATCTGGGCCACGACGTCGTCGTCCGCG
ATCCCGACTATCCGGCTTCGACCTATGCCAACTACCTGCCCCGCTTTTTCCGCGGTATCAGCGACGACGCGGACGCGCAG
GCGCACCCGGACCGCCTCGAAGCACGTACCCGAGCCATAGCGCGTCTAGGGTCGTTCTTCTCCGACCGGCGGATGGCGGC
CCTGCGGGCCGCCGAGGTGGTGCTGAGCAGCCGGATCCAGTCGATCTTCGACGATGTCGACGTAGTTGTGACGCCAGGCG
CCGCGACCGGCCCGTCCCGCATCGGCGCCTACCAACGCCGGGGTGCAGTTTCGACGTTGCTGCTGGTGGTGCAGCGGGTT
CCGTACTTTCAAGTCTGGAATCTGACCGGCCAGCCCGCGGCCGTGGTGCCGTGGGACTTCGACGGCGACGGCCTGCCCAT
GTCGGTTCAACTCGTCGGCCGGCCGTATGACGAGGCGACGCTGCTGGCACTGGCCGCACAGATCGAATCTGCCAGACCCT
GGGCCCATCGGCGGCCGTCGGTGTCATGA

Upstream 100 bases:

>100_bases
CGGAGCCTCCCCGGCGATGATCGCACAGAACACGCAGGGCATGTCGCTACGGTACTGGACCTCTCGGAGACCGCCCAAGT
GAACGGGATACGCTGCCGCC

Downstream 100 bases:

>100_bases
CATTGCAGTCGCCCGCTCGTTTTTCACGTTTTTGCCCGGCCGCAGGACATGTGCGGCGGCGTTAACGTTGACTGGTGACA
GACCACGTGCGCGAGGCGGA

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAI
KDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSA
AAVAAGLAPVALGSDGGGSIRIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGE
FVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPASTYANYLPRFFRGISDDADAQ
AHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRV
PYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS

Sequences:

>Translated_462_residues
MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAI
KDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSA
AAVAAGLAPVALGSDGGGSIRIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGE
FVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPASTYANYLPRFFRGISDDADAQ
AHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRV
PYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS
>Mature_462_residues
MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAI
KDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSA
AAVAAGLAPVALGSDGGGSIRIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGE
FVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPASTYANYLPRFFRGISDDADAQ
AHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRV
PYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI195972892, Length=246, Percent_Identity=29.6747967479675, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=24.6987951807229, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI166795287, Length=453, Percent_Identity=29.5805739514349, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=489, Percent_Identity=25.3578732106339, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=221, Percent_Identity=33.4841628959276, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=221, Percent_Identity=32.1266968325792, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=467, Percent_Identity=25.2676659528908, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=416, Percent_Identity=27.1634615384615, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32565337, Length=489, Percent_Identity=23.1083844580777, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=137, Percent_Identity=40.8759124087591, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=137, Percent_Identity=40.8759124087591, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=265, Percent_Identity=29.0566037735849, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=385, Percent_Identity=26.2337662337662, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=173, Percent_Identity=36.9942196531792, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21356731, Length=182, Percent_Identity=39.5604395604396, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648435, Length=472, Percent_Identity=25, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648437, Length=472, Percent_Identity=25, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI45550774, Length=472, Percent_Identity=25, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648441, Length=410, Percent_Identity=26.3414634146341, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648439, Length=410, Percent_Identity=26.3414634146341, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=26.219512195122, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=26.219512195122, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=26.219512195122, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=26.219512195122, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=226, Percent_Identity=31.4159292035398, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161078093, Length=231, Percent_Identity=35.0649350649351, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=30.0411522633745, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIB2_MYCBO (P63493)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_854948.1
- ProteinModelPortal:   P63493
- SMR:   P63493
- EnsemblBacteria:   EBMYCT00000015092
- GeneID:   1092091
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1294
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   LLEVCES
- ProtClustDB:   PRK12470
- BioCyc:   MBOV233413:MB1294-MONOMER
- BRENDA:   3.5.1.4
- InterPro:   IPR000120
- InterPro:   IPR020556
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: =3.5.1.4

Molecular weight: Translated: 49081; Mature: 49081

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 81-81 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEA
CCCCHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQRLDAGERLPLLGVPIAIKDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVII
HHHHHCCCCCCCEEECCEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCEEEE
GKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSAAAVAAGLAPVALGSDGGGSI
ECCCCCCEEEECCHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCEEECCCCCCEE
RIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGE
EEEEEEEHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
FVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPAS
EEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
TYANYLPRFFRGISDDADAQAHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRVPYFQVWNLTGQPAAVVPWDF
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCC
DGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEA
CCCCHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQRLDAGERLPLLGVPIAIKDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVII
HHHHHCCCCCCCEEECCEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCEEEE
GKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSAAAVAAGLAPVALGSDGGGSI
ECCCCCCEEEECCHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCEEECCCCCCEE
RIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGE
EEEEEEEHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
FVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPAS
EEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHH
TYANYLPRFFRGISDDADAQAHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRVPYFQVWNLTGQPAAVVPWDF
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCC
DGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972