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The map label for this gene is fadD21

Identifier: 121637116

GI number: 121637116

Start: 1357060

End: 1358796

Strand: Reverse

Name: fadD21

Synonym: BCG_1247c

Alternate gene names: 121637116

Gene position: 1358796-1357060 (Counterclockwise)

Preceding gene: 121637117

Following gene: 121637115

Centisome position: 31.06

GC content: 59.93

Gene sequence:

>1737_bases
ATGTCCGACTCTTCTGTCTTGTCCTTGCTGCGCGAGCGTGCCGGCCTGCAGCCCGACGACGCCGCGTTCACGTACATCGA
CTACGAACAGGATTGGGCCGGCATCACCGAGACGCTCACGTGGTCAGAGGTGTTTCGACGGACCCGCATCGTTGCTCACG
AAGTCAGGCGGCATTGCACGACCGGAGACCGAGCCGTGATTCTGGCTCCGCAGGGCCTGGCCTACATCGCGGCTTTCCTA
GGTTCGATGCAGGCCGGAGCGATAGCGGTTCCGTTGTCAGTCCCGCAGATCGGCTCTCACGACGAACGTGTCAGCGCGGT
GCTTGCCGACGCGTCACCTTCGGTCATCCTGACAACATCTGCGGTCGCAGAAGCGGTTGCCGAGCACATCCACCGGCCGA
ACACGAACAACGTAGGGCCGATCATCGAAATCGATTCATTGGACTTGACCGGGAATTCACCGAGCTTTCGGGTGAAGGAT
CTGCCAAGCGCCGCGTACTTGCAGTACACCTCTGGCTCCACCCGCGCTCCGGCTGGGGTCATGATCTCGCATCGGAACCT
TCAGGCGAATTTCCAACAGCTGATGTCGAACTACTTCGGCGACCGCAACGGTGTGGCGCCACCTGACACTACCATCGTGT
CGTGGCTGCCGTTCTACCACGACATGGGCTTGGTCCTTGGCATCATCGCACCGATCCTGGGCGGCTACCGCAGTGAACTC
ACTAGCCCGCTGGCGTTCCTGCAACGGCCAGCGCGTTGGCTACATTCGCTGGCCAACGGAAGTCCTTCGTGGTCGGCCGC
ACCGAATTTCGCCTTCGAATTGGCCGTCCGCAAGACAACAGATGCCGACATAGAAGGCCTCGACCTCGGCAACGTCCTGG
GCATCACCAGTGGTGCTGAACGAGTCCACCCCAACACGCTCAGTAGATTCTGCAACCGGTTTGCACCGTACAATTTCCGC
GAAGACATGATACGGCCTTCGTACGGTCTGGCGGAAGCGACTCTTTATGTGGCCAGCCGCAATTCGGGTGACAAGCCAGA
AGTCGTCTACTTCGAACCTGACAAGCTGTCCACGGGCAGTGCGAATCGATGTGAACCCAAGACCGGCACACCGCTGCTGA
GCTATGGCATGCCGACATCACCAACAGTGCGAATTGTGGATCCCGACACCTGCATTGAGTGCCCCGCGGGAACGATCGGC
GAGATCTGGGTGAAGGGCGACAACGTCGCCGAAGGCTACTGGAACAAACCGGACGAGACACGGCACACCTTTGGCGCAAT
GCTGGTCCACCCCTCGGCAGGTACTCCTGATGGATCCTGGTTGCGAACGGGAGACCTCGGATTCCTCTCCGAGGACGAGA
TGTTCATCGTGGGCCGTATGAAGGACATGCTGATCGTATACGGGCGCAATCACTACCCCGAGGACATCGAGTCGACCGTC
CAGGAGATCACTGGTGGTCGGGTCGCTGCGATTTCAGTTCCGGTCGATCACACAGAGAAGTTAGTCACTGTCATCGAGCT
CAAGCTCCTCGGCGATTCCGCCGGCGAAGCGATGGACGAACTCGATGTCATCAAGAACAACGTCACAGCCGCGATATCAC
GCTCGCACGGTCTCAACGTGGCGGACCTCGTCTTGGTCCCGCCCGGGTCGATCCCCACCACAACCAGCGGCAAGATCAGA
AGGGCCGCCTGTGTAGAGCAATACCGGCTGCAGCAATTCACCCGGTTGGACGGCTGA

Upstream 100 bases:

>100_bases
GCACAGCATTGCAGAACGTTATGATCAGCATCGCTATCGCCAACCATCCCCCTGCCCGCTTGGGTTATCGGAGATCGCGC
GAAACGGTAATGTAGCACCA

Downstream 100 bases:

>100_bases
GCTAGTCGGGGCTGACCCAGGGCGATTCACAGCTTGTTGGCTGCCAACTTTAATGTCGGCCGGGACGTGGCTGACTAAGA
TATGGCGACATAACCGCCCG

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSDSSVLSLLRERAGLQPDDAAFTYIDYEQDWAGITETLTWSEVFRRTRIVAHEVRRHCTTGDRAVILAPQGLAYIAAFL
GSMQAGAIAVPLSVPQIGSHDERVSAVLADASPSVILTTSAVAEAVAEHIHRPNTNNVGPIIEIDSLDLTGNSPSFRVKD
LPSAAYLQYTSGSTRAPAGVMISHRNLQANFQQLMSNYFGDRNGVAPPDTTIVSWLPFYHDMGLVLGIIAPILGGYRSEL
TSPLAFLQRPARWLHSLANGSPSWSAAPNFAFELAVRKTTDADIEGLDLGNVLGITSGAERVHPNTLSRFCNRFAPYNFR
EDMIRPSYGLAEATLYVASRNSGDKPEVVYFEPDKLSTGSANRCEPKTGTPLLSYGMPTSPTVRIVDPDTCIECPAGTIG
EIWVKGDNVAEGYWNKPDETRHTFGAMLVHPSAGTPDGSWLRTGDLGFLSEDEMFIVGRMKDMLIVYGRNHYPEDIESTV
QEITGGRVAAISVPVDHTEKLVTVIELKLLGDSAGEAMDELDVIKNNVTAAISRSHGLNVADLVLVPPGSIPTTTSGKIR
RAACVEQYRLQQFTRLDG

Sequences:

>Translated_578_residues
MSDSSVLSLLRERAGLQPDDAAFTYIDYEQDWAGITETLTWSEVFRRTRIVAHEVRRHCTTGDRAVILAPQGLAYIAAFL
GSMQAGAIAVPLSVPQIGSHDERVSAVLADASPSVILTTSAVAEAVAEHIHRPNTNNVGPIIEIDSLDLTGNSPSFRVKD
LPSAAYLQYTSGSTRAPAGVMISHRNLQANFQQLMSNYFGDRNGVAPPDTTIVSWLPFYHDMGLVLGIIAPILGGYRSEL
TSPLAFLQRPARWLHSLANGSPSWSAAPNFAFELAVRKTTDADIEGLDLGNVLGITSGAERVHPNTLSRFCNRFAPYNFR
EDMIRPSYGLAEATLYVASRNSGDKPEVVYFEPDKLSTGSANRCEPKTGTPLLSYGMPTSPTVRIVDPDTCIECPAGTIG
EIWVKGDNVAEGYWNKPDETRHTFGAMLVHPSAGTPDGSWLRTGDLGFLSEDEMFIVGRMKDMLIVYGRNHYPEDIESTV
QEITGGRVAAISVPVDHTEKLVTVIELKLLGDSAGEAMDELDVIKNNVTAAISRSHGLNVADLVLVPPGSIPTTTSGKIR
RAACVEQYRLQQFTRLDG
>Mature_577_residues
SDSSVLSLLRERAGLQPDDAAFTYIDYEQDWAGITETLTWSEVFRRTRIVAHEVRRHCTTGDRAVILAPQGLAYIAAFLG
SMQAGAIAVPLSVPQIGSHDERVSAVLADASPSVILTTSAVAEAVAEHIHRPNTNNVGPIIEIDSLDLTGNSPSFRVKDL
PSAAYLQYTSGSTRAPAGVMISHRNLQANFQQLMSNYFGDRNGVAPPDTTIVSWLPFYHDMGLVLGIIAPILGGYRSELT
SPLAFLQRPARWLHSLANGSPSWSAAPNFAFELAVRKTTDADIEGLDLGNVLGITSGAERVHPNTLSRFCNRFAPYNFRE
DMIRPSYGLAEATLYVASRNSGDKPEVVYFEPDKLSTGSANRCEPKTGTPLLSYGMPTSPTVRIVDPDTCIECPAGTIGE
IWVKGDNVAEGYWNKPDETRHTFGAMLVHPSAGTPDGSWLRTGDLGFLSEDEMFIVGRMKDMLIVYGRNHYPEDIESTVQ
EITGGRVAAISVPVDHTEKLVTVIELKLLGDSAGEAMDELDVIKNNVTAAISRSHGLNVADLVLVPPGSIPTTTSGKIRR
AACVEQYRLQQFTRLDG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI44888818, Length=553, Percent_Identity=24.4122965641953, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI45827692, Length=585, Percent_Identity=24.4444444444444, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI55749758, Length=575, Percent_Identity=25.7391304347826, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI225735629, Length=585, Percent_Identity=24.4444444444444, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI225735625, Length=611, Percent_Identity=24.2225859247136, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI45827694, Length=551, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI225735627, Length=516, Percent_Identity=23.8372093023256, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI45827696, Length=516, Percent_Identity=23.8372093023256, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=524, Percent_Identity=25.1908396946565, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193202679, Length=566, Percent_Identity=25.2650176678445, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=506, Percent_Identity=25.4940711462451, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=518, Percent_Identity=22.7799227799228, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI18859661, Length=442, Percent_Identity=26.6968325791855, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24654656, Length=474, Percent_Identity=23.4177215189873, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FAD21_MYCBO (P63524)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_854871.1
- ProteinModelPortal:   P63524
- EnsemblBacteria:   EBMYCT00000014613
- GeneID:   1090507
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1217c
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   SPYKTLT
- ProtClustDB:   PRK05850
- BioCyc:   MBOV233413:MB1217C-MONOMER
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62757; Mature: 62626

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSSVLSLLRERAGLQPDDAAFTYIDYEQDWAGITETLTWSEVFRRTRIVAHEVRRHCT
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TGDRAVILAPQGLAYIAAFLGSMQAGAIAVPLSVPQIGSHDERVSAVLADASPSVILTTS
CCCCEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHEEEECCCCCEEEEHH
AVAEAVAEHIHRPNTNNVGPIIEIDSLDLTGNSPSFRVKDLPSAAYLQYTSGSTRAPAGV
HHHHHHHHHHCCCCCCCCCCEEEEECEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCE
MISHRNLQANFQQLMSNYFGDRNGVAPPDTTIVSWLPFYHDMGLVLGIIAPILGGYRSEL
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TSPLAFLQRPARWLHSLANGSPSWSAAPNFAFELAVRKTTDADIEGLDLGNVLGITSGAE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECCCCCC
RVHPNTLSRFCNRFAPYNFREDMIRPSYGLAEATLYVASRNSGDKPEVVYFEPDKLSTGS
CCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCC
ANRCEPKTGTPLLSYGMPTSPTVRIVDPDTCIECPAGTIGEIWVKGDNVAEGYWNKPDET
CCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEECCCCCCCCHH
RHTFGAMLVHPSAGTPDGSWLRTGDLGFLSEDEMFIVGRMKDMLIVYGRNHYPEDIESTV
HHHHEEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHH
QEITGGRVAAISVPVDHTEKLVTVIELKLLGDSAGEAMDELDVIKNNVTAAISRSHGLNV
HHHCCCEEEEEEECCCCHHHHEEEEEEEEECCCHHHHHHHHHHHHCCHHEEEECCCCCCE
ADLVLVPPGSIPTTTSGKIRRAACVEQYRLQQFTRLDG
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSSVLSLLRERAGLQPDDAAFTYIDYEQDWAGITETLTWSEVFRRTRIVAHEVRRHCT
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TGDRAVILAPQGLAYIAAFLGSMQAGAIAVPLSVPQIGSHDERVSAVLADASPSVILTTS
CCCCEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHEEEECCCCCEEEEHH
AVAEAVAEHIHRPNTNNVGPIIEIDSLDLTGNSPSFRVKDLPSAAYLQYTSGSTRAPAGV
HHHHHHHHHHCCCCCCCCCCEEEEECEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCE
MISHRNLQANFQQLMSNYFGDRNGVAPPDTTIVSWLPFYHDMGLVLGIIAPILGGYRSEL
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TSPLAFLQRPARWLHSLANGSPSWSAAPNFAFELAVRKTTDADIEGLDLGNVLGITSGAE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECCCCCC
RVHPNTLSRFCNRFAPYNFREDMIRPSYGLAEATLYVASRNSGDKPEVVYFEPDKLSTGS
CCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCC
ANRCEPKTGTPLLSYGMPTSPTVRIVDPDTCIECPAGTIGEIWVKGDNVAEGYWNKPDET
CCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEECCCCCCCCHH
RHTFGAMLVHPSAGTPDGSWLRTGDLGFLSEDEMFIVGRMKDMLIVYGRNHYPEDIESTV
HHHHEEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHH
QEITGGRVAAISVPVDHTEKLVTVIELKLLGDSAGEAMDELDVIKNNVTAAISRSHGLNV
HHHCCCEEEEEEECCCCHHHHEEEEEEEEECCCHHHHHHHHHHHHCCHHEEEECCCCCCE
ADLVLVPPGSIPTTTSGKIRRAACVEQYRLQQFTRLDG
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972