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The map label for this gene is cobB

Identifier: 121637082

GI number: 121637082

Start: 1310320

End: 1311033

Strand: Reverse

Name: cobB

Synonym: BCG_1212c

Alternate gene names: 121637082

Gene position: 1311033-1310320 (Counterclockwise)

Preceding gene: 121637084

Following gene: 121637080

Centisome position: 29.97

GC content: 66.81

Gene sequence:

>714_bases
ATGCGAGTGGCGGTGCTCAGCGGCGCGGGGATCTCCGCGGAAAGCGGTGTACCGACGTTCCGCGATGACAAGAACGGATT
GTGGGCCCGATTCGACCCTTACGAGCTGTCCAGCACGCAAGGCTGGCTGCGCAACCCCGAGCGGGTCTGGGGATGGTACC
TATGGCGCCATTATCTGGTGGCCAACGTCGAACCCAACGACGGGCATCGCGCGATCGCCGCCTGGCAGGACCATGCTGAG
GTCAGCGTCATCACCCAGAATGTCGACGATCTGCACGAGCGCGCCGGCAGCGGTGCGGTGCATCACCTGCACGGCAGCCT
TTTCGAATTTCGTTGTGCACGTTGTGGTGTGCCCTACACCGACGCCCTTCCGGAGATGCCCGAGCCTGCGATCGAAGTGG
AGCCGCCGGTCTGCGACTGCGGCGGTCTGATCCGGCCCGACATCGTATGGTTCGGTGAGCCGCTGCCAGAGGAGCCGTGG
CGGAGCGCGGTCGAGGCGACAGGGAGCGCCGACGTGATGGTCGTGGTGGGGACCTCGGCGATCGTCTACCCGGCGGCCGG
TTTACCCGACCTGGCGCTGGCGCGCGGCACTGCCGTGATCGAAGTCAATCCCGAGCCCACGCCGTTGTCCGGCAGCGCGA
CGATCAGCATCCGCGAGTCGGCCAGCCAAGCGTTGCCGGGGCTGTTGGAGCGCCTGCCCGCCCTGCTGAAATAG

Upstream 100 bases:

>100_bases
GAGCTGGTCGAACAACGGCTTTCCCGCCTTCACGTCGACCCGTAACCAATCCCGCAGCTCCACGTACCCAGTATCTACCA
AGCACAGCTATGTTTGTGGG

Downstream 100 bases:

>100_bases
TCGCCTATGGGCGCCGAACGTGAACTCAGGGCGACGGTTTGCCGGGCGGAATCTCGCCCTGGATGCACGCTCGCCGGTCT
CGGCTAGGGCGTGTCTCCCA

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRVAVLSGAGISAESGVPTFRDDKNGLWARFDPYELSSTQGWLRNPERVWGWYLWRHYLVANVEPNDGHRAIAAWQDHAE
VSVITQNVDDLHERAGSGAVHHLHGSLFEFRCARCGVPYTDALPEMPEPAIEVEPPVCDCGGLIRPDIVWFGEPLPEEPW
RSAVEATGSADVMVVVGTSAIVYPAAGLPDLALARGTAVIEVNPEPTPLSGSATISIRESASQALPGLLERLPALLK

Sequences:

>Translated_237_residues
MRVAVLSGAGISAESGVPTFRDDKNGLWARFDPYELSSTQGWLRNPERVWGWYLWRHYLVANVEPNDGHRAIAAWQDHAE
VSVITQNVDDLHERAGSGAVHHLHGSLFEFRCARCGVPYTDALPEMPEPAIEVEPPVCDCGGLIRPDIVWFGEPLPEEPW
RSAVEATGSADVMVVVGTSAIVYPAAGLPDLALARGTAVIEVNPEPTPLSGSATISIRESASQALPGLLERLPALLK
>Mature_237_residues
MRVAVLSGAGISAESGVPTFRDDKNGLWARFDPYELSSTQGWLRNPERVWGWYLWRHYLVANVEPNDGHRAIAAWQDHAE
VSVITQNVDDLHERAGSGAVHHLHGSLFEFRCARCGVPYTDALPEMPEPAIEVEPPVCDCGGLIRPDIVWFGEPLPEEPW
RSAVEATGSADVMVVVGTSAIVYPAAGLPDLALARGTAVIEVNPEPTPLSGSATISIRESASQALPGLLERLPALLK

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI6912664, Length=250, Percent_Identity=40, Blast_Score=161, Evalue=4e-40,
Organism=Homo sapiens, GI13787215, Length=229, Percent_Identity=42.3580786026201, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI300795542, Length=243, Percent_Identity=37.8600823045267, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI6912662, Length=257, Percent_Identity=27.6264591439689, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI6912660, Length=197, Percent_Identity=30.4568527918782, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI13775602, Length=197, Percent_Identity=29.9492385786802, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI13775600, Length=197, Percent_Identity=29.9492385786802, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI7657575, Length=227, Percent_Identity=27.3127753303965, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI63054862, Length=193, Percent_Identity=29.5336787564767, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI300797705, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI308199517, Length=247, Percent_Identity=36.8421052631579, Blast_Score=137, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71990482, Length=261, Percent_Identity=29.5019157088123, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17567771, Length=264, Percent_Identity=26.1363636363636, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17541892, Length=216, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=263, Percent_Identity=29.277566539924, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325242, Length=215, Percent_Identity=31.6279069767442, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571445, Length=192, Percent_Identity=32.8125, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648389, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_MYCBO (P66814)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854837.1
- ProteinModelPortal:   P66814
- SMR:   P66814
- EnsemblBacteria:   EBMYCT00000016460
- GeneID:   1090474
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1182c
- GeneTree:   EBGT00050000016307
- HOGENOM:   HBG641281
- OMA:   DADGLWE
- ProtClustDB:   PRK00481
- BioCyc:   MBOV233413:MB1182C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25661; Mature: 25661

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAVLSGAGISAESGVPTFRDDKNGLWARFDPYELSSTQGWLRNPERVWGWYLWRHYLV
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEEE
ANVEPNDGHRAIAAWQDHAEVSVITQNVDDLHERAGSGAVHHLHGSLFEFRCARCGVPYT
EECCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCH
DALPEMPEPAIEVEPPVCDCGGLIRPDIVWFGEPLPEEPWRSAVEATGSADVMVVVGTSA
HCCCCCCCCCEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCE
IVYPAAGLPDLALARGTAVIEVNPEPTPLSGSATISIRESASQALPGLLERLPALLK
EEECCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVAVLSGAGISAESGVPTFRDDKNGLWARFDPYELSSTQGWLRNPERVWGWYLWRHYLV
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEEE
ANVEPNDGHRAIAAWQDHAEVSVITQNVDDLHERAGSGAVHHLHGSLFEFRCARCGVPYT
EECCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCH
DALPEMPEPAIEVEPPVCDCGGLIRPDIVWFGEPLPEEPWRSAVEATGSADVMVVVGTSA
HCCCCCCCCCEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCE
IVYPAAGLPDLALARGTAVIEVNPEPTPLSGSATISIRESASQALPGLLERLPALLK
EEECCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972