BacMap

Definition	Mycobacterium bovis BCG str. Pasteur 1173P2, complete genome.
Accession	NC_008769
Length	4,374,522

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The map label for this gene is echA8

Identifier: 121636999

GI number: 121636999

Start: 1225569

End: 1226342

Strand: Reverse

Name: echA8

Synonym: BCG_1128c

Alternate gene names: 121636999

Gene position: 1226342-1225569 (Counterclockwise)

Preceding gene: 121637000

Following gene: 121636998

Centisome position: 28.03

GC content: 64.6

Gene sequence:

>774_bases
ATGACGTACGAAACCATCCTGGTCGAGCGCGATCAGCGAGTTGGCATTATCACGCTGAACCGTCCCCAGGCACTGAACGC
GCTCAACAGCCAGGTGATGAACGAGGTCACCAGCGCTGCAACCGAACTGGACGATGACCCGGACATTGGGGCGATCATCA
TCACCGGTTCGGCCAAAGCGTTTGCCGCCGGAGCCGACATCAAAGAAATGGCCGACCTGACGTTCGCCGACGCGTTCACC
GCCGACTTCTTCGCCACCTGGGGCAAGCTGGCCGCCGTGCGCACCCCGACGATCGCCGCGGTGGCGGGATACGCGCTCGG
CGGTGGCTGCGAGCTGGCGATGATGTGCGACGTGCTGATCGCCGCCGACACCGCGAAGTTCGGACAGCCCGAGATAAAGC
TGGGCGTGCTGCCAGGCATGGGCGGCTCCCAGCGGCTGACCCGGGCTATCGGCAAGGCTAAGGCGATGGACCTCATCCTG
ACCGGGCGCACCATGGACGCCGCCGAGGCCGAGCGCAGCGGTCTGGTTTCACGGGTGGTGCCGGCCGACGACTTGCTGAC
CGAAGCCAGGGCCACTGCCACGACCATTTCGCAGATGTCGGCCTCGGCGGCCCGGATGGCCAAGGAGGCCGTCAACCGGG
CTTTCGAATCCAGTTTGTCCGAGGGGCTGCTCTACGAACGCCGGCTTTTCCATTCGGCTTTCGCGACCGAAGACCAATCC
GAAGGTATGGCAGCGTTCATCGAGAAACGCGCTCCCCAGTTCACCCACCGATGA

Upstream 100 bases:

>100_bases
CGAACTGGTCGCCGGCAACCCTGGACGCGATCACGGCGGCCGACATCGAAGCCTATTTCGAACCGGTCGACGATGACTTG
AGTTTCTAGAGAGGTAGCGC

Downstream 100 bases:

>100_bases
CGGAACCGGCTGCAGCGACGACCACCAACGCCAGCGACGAACCAGCTACCGGCGCTGAGCAGGCGGTCGACACCGCAGCA
ACTCCACAAACCCCTGAACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTYETILVERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKEMADLTFADAFT
ADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQS
EGMAAFIEKRAPQFTHR

Sequences:

>Translated_257_residues
MTYETILVERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKEMADLTFADAFT
ADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQS
EGMAAFIEKRAPQFTHR
>Mature_256_residues
TYETILVERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKEMADLTFADAFTA
DFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILT
GRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQSE
GMAAFIEKRAPQFTHR

Specific function: Could possibly oxidize fatty acids using specific components

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=50.390625, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=36.4705882352941, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=29.1497975708502, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=32.5396825396825, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=31.6981132075472, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI68989263, Length=226, Percent_Identity=32.3008849557522, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=30.8510638297872, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=30.8510638297872, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=27.6595744680851, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=27.6595744680851, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=23.3463035019455, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=23.3463035019455, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=23.3463035019455, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=37.7777777777778, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI157694520, Length=219, Percent_Identity=26.027397260274, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=265, Percent_Identity=23.7735849056604, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI49355787, Length=265, Percent_Identity=23.7735849056604, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI25453481, Length=264, Percent_Identity=23.4848484848485, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI51538573, Length=264, Percent_Identity=23.4848484848485, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4757966, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI62530384, Length=224, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=189, Percent_Identity=30.1587301587302, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=46.09375, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.9230769230769, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=36.0153256704981, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=32.5670498084291, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=34.8484848484849, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=33.1521739130435, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=52.1400778210117, Blast_Score=277, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=49.1803278688525, Blast_Score=252, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=33.0396475770925, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=32.3651452282158, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=27.3809523809524, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=276, Percent_Identity=27.8985507246377, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558304, Length=233, Percent_Identity=29.1845493562232, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=29.6875, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=33.4905660377358, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=231, Percent_Identity=30.3030303030303, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=231, Percent_Identity=30.3030303030303, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=231, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25144157, Length=283, Percent_Identity=28.2685512367491, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=283, Percent_Identity=28.2685512367491, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25.390625, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=185, Percent_Identity=27.5675675675676, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=28.4153005464481, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=53.4883720930233, Blast_Score=285, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=53.4883720930233, Blast_Score=285, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=34.2307692307692, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=27.1317829457364, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=29.4573643410853, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24650670, Length=258, Percent_Identity=30.2325581395349, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=31.7269076305221, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24654903, Length=221, Percent_Identity=28.0542986425339, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=29.7752808988764, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=29.7752808988764, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583165, Length=173, Percent_Identity=29.4797687861272, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921000, Length=202, Percent_Identity=32.6732673267327, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24583077, Length=202, Percent_Identity=32.6732673267327, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583079, Length=202, Percent_Identity=32.6732673267327, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921018, Length=181, Percent_Identity=27.0718232044199, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ECHA8_MYCBO (P64017)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854754.1
- ProteinModelPortal:   P64017
- SMR:   P64017
- EnsemblBacteria:   EBMYCT00000017177
- GeneID:   1092072
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1099c
- GeneTree:   EBGT00050000015506
- HOGENOM:   HBG748731
- OMA:   YETTLTE
- ProtClustDB:   PRK05862
- BioCyc:   MBOV233413:MB1099C-MONOMER
- BRENDA:   4.2.1.17
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 27273; Mature: 27142

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYETILVERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKA
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHH
FAAGADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVV
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCHHHCC
PADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMAAFIEKRAPQFTHR
CHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TYETILVERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKA
CCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHH
FAAGADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVV
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCHHHCC
PADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMAAFIEKRAPQFTHR
CHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972