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The map label for this gene is ptrB [C]

Identifier: 121636372

GI number: 121636372

Start: 578401

End: 580431

Strand: Reverse

Name: ptrB [C]

Synonym: BCG_0497c

Alternate gene names: 121636372

Gene position: 580431-578401 (Counterclockwise)

Preceding gene: 121636379

Following gene: 121636371

Centisome position: 13.27

GC content: 64.25

Gene sequence:

>2031_bases
ATGGCTTCCATGACATTTGAGCCTGCCCCAGACGGCGCTGACCCCTACCTATGGCTCGAGGACGTCACCGGTGCGGAAGC
GCTGGATTGGGTACGCGCGCGCAACAAGCCGACAACGGCGGCGTTCTGCGACGCAGAGTTCGAGCGGATGCGCGTCGAGG
CGCTCGAGGTGCTCGACACCGATGCCCGAATCCCCTATGTGAACAGGCGCGGCAACTATCTCTACAACTTCTGGCGCGAT
GCCGCCAACCCCCGCGGGTTGTGGCGGCGCACCACGCTGGACAGCTACCGCACCGACTCGCCGGGCTGGGATGTGCTGAT
CGATGTTGATGAACTGGGCCGCGCCGACGACCAGAAGTGGGTGTGGGGCGGCGCCGGTGTCATCGAACCCGACTACACGC
GCGCGCTGATCGGCCTGTCCCCGGGCGGCTCGGATGCATCCATTGTGCGTGAATTCGACATGCTGACACGCGAATTCGTC
GAGGATGGGTTCCAGCTGCCACCGGCCAAATCCCAGATTACCTGGGAGGACCCGGACACTGTCTTGCTGGGCACCGACTT
CGGCGGCGACTCGCTCACCACTTCCGGCTATCCGCGAGTGATCAAGCGATGGCGCCGGGGCAAACCGCTGGCCGATGCGG
AGACAATCTTCGAGGGCGCGGGCACCGACGTCCGCGTCAACGCGTCAGCGGATCGCACGCCCGGATTCGAGCGCACCCTG
CTGGGACGGGCCCTCGACTTCTGGAACGAAGAGGTCTACGAATTGCGTGGTTCGGAGCTGATCCGAATCGAAGCCCCCAC
CGACGCGAGCGTGTCAATTCACCGCGACTGGCTGTTGATCGAGCTGCGCACCGACTGGACCGTGGCCACCACCAGGTACA
CTGCCGGCTCACTGCTGGCCGCCGAATACGACGAATTCCTTGCCGGCTCAGCGGAATTACAGGTGGTGTTCGAACCCGAC
GAGCACACCGCGCTGTACCAATACGCCTGGACCCGAGACCGGCTGTTGATCGTCACCCTGGCCGACGTGGCGAGCCGCGT
CGAGATCGCAACTCCCGGCAGCTGGCGACGTGAGCCACTCTCAGGTATCCCCGCCGCCACCAACACCGTCATCGTCTCCG
CCGACAGCCACGGCGACGAGTTCTTCCTCGACTCCAGTGGATTCGATACGCCGTCGCGGCTGATGCGCGGCACCGATGAC
GGCCGACTAGCAGAGATCAAATCTGCGCCAGCTTTCTTCGACGCGGAAAACATGGCTGTAACACAGTATTTCGCGACATC
AGACGATGGCACATCGATCCCGTATTTCGTCGTGCGACGCACCGACGCGGACAATCCCGGCCCCACATTGTTGAACGGCT
ACGGTGGATTCGAGACATCCAGAACGCCGACGTATGACGGCGTCCTGGGCCGGCTGTGGCTGGCCCGCGGGGGCACCTAT
GCGTTGGCCAACATCCGCGGCGGCGGCGAGTACGGGCCTGGCTGGCACACGCAGGCGATGCGCGAGGGCCGGGACAAGGT
CGCCCAAGACTTCGCCGCCGTGGCAACCGATTTGGTGACCCGCGGCATCACCACCGCTGAGCAGCTCGGCGCGCGGGGCG
GCAGCAACGGTGGGCTGCTGATGGGCATCATGTTGACCGGGTACCCGGAGAAGTTCGGCGCGCTGGTCTGCGACGTGCCG
CTGCTGGACATGAAGCGGTACCACCTGCTGCTGGCCGGCGCTTCCTGGATGGCCGAATACGGCGATCCGGATAACCCGGA
TGACTGGAAGTTCATCTCTGAATATTCGCCATACCAAAACATTTCGGCGAACCGGAAATACCCGCCAGTACTCATGACGA
CCTCTACCCGCGACGATCGGGTGCACCCCGGCCACGCCCGCAAAATGACGGCGGCCCTGCAGGCTGCGGGCCACCCCGTC
TGGTATTACGAAAACATCGAGGGTGGGCACGCCGGCGCAGCCGACAACGCACAGATCGCATTCAAATCGGCGCTGAGTTT
CGCGTTCCTATGGCGGATGCTGGCCGGCTAA

Upstream 100 bases:

>100_bases
CATCAGCGCCCCGGCGGAACCGGGACGGGAAAAGACAGTCATCGTATTCGGCTCCTCGTCAAAATCATGTAATACAGCTC
ACACTACCGCCGGGGCCACA

Downstream 100 bases:

>100_bases
CCGGCATTCACGAACGGTGGACTTAGCCCAAATAACGTGGCTATCGAGTTGTAGCCGATTGATACTGCATTACTACCTAT
TACTACGTATTACTATCTGT

Product: putative peptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 676; Mature: 675

Protein sequence:

>676_residues
MASMTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDARIPYVNRRGNYLYNFWRD
AANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFV
EDGFQLPPAKSQITWEDPDTVLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTL
LGRALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEYDEFLAGSAELQVVFEPD
EHTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDD
GRLAEIKSAPAFFDAENMAVTQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTY
ALANIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGIMLTGYPEKFGALVCDVP
LLDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPV
WYYENIEGGHAGAADNAQIAFKSALSFAFLWRMLAG

Sequences:

>Translated_676_residues
MASMTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDARIPYVNRRGNYLYNFWRD
AANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFV
EDGFQLPPAKSQITWEDPDTVLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTL
LGRALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEYDEFLAGSAELQVVFEPD
EHTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDD
GRLAEIKSAPAFFDAENMAVTQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTY
ALANIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGIMLTGYPEKFGALVCDVP
LLDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPV
WYYENIEGGHAGAADNAQIAFKSALSFAFLWRMLAG
>Mature_675_residues
ASMTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDARIPYVNRRGNYLYNFWRDA
ANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVE
DGFQLPPAKSQITWEDPDTVLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTLL
GRALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEYDEFLAGSAELQVVFEPDE
HTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDG
RLAEIKSAPAFFDAENMAVTQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTYA
LANIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGIMLTGYPEKFGALVCDVPL
LDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVW
YYENIEGGHAGAADNAQIAFKSALSFAFLWRMLAG

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=388, Percent_Identity=30.1546391752577, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI284172438, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI284172431, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI284172420, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI284172413, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI70778815, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI108860686, Length=203, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI108860692, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788150, Length=299, Percent_Identity=28.4280936454849, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583414, Length=318, Percent_Identity=36.1635220125786, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI221510989, Length=240, Percent_Identity=39.5833333333333, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 74754; Mature: 74623

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDT
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCC
DARIPYVNRRGNYLYNFWRDAANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKW
CCCCCEEECCCCEEHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCE
VWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDGFQLPPAKSQITWEDPDT
EECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE
VLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTL
EEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHH
LGRALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCEEEEEEECCEEEEEEEECCCCHHH
AEYDEFLAGSAELQVVFEPDEHTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPL
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECHHHCEEEEECCCCCCCCCC
SGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRLAEIKSAPAFFDAENMAV
CCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCCEEEECCCCCEECCCCEEE
TQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTY
EEEEEECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEE
ALANIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLL
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEE
MGIMLTGYPEKFGALVCDVPLLDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQN
EEEEEECCCHHHCCEEECCCHHCCCEEEEEEECHHHHHHCCCCCCCCCCHHHHCCCCCCC
ISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYYENIEGGHAGAADNAQIA
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHH
FKSALSFAFLWRMLAG
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASMTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDT
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCC
DARIPYVNRRGNYLYNFWRDAANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKW
CCCCCEEECCCCEEHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCE
VWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDGFQLPPAKSQITWEDPDT
EECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE
VLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTL
EEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHH
LGRALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLA
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCEEEEEEECCEEEEEEEECCCCHHH
AEYDEFLAGSAELQVVFEPDEHTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPL
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECHHHCEEEEECCCCCCCCCC
SGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRLAEIKSAPAFFDAENMAV
CCCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCCEEEECCCCCEECCCCEEE
TQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTY
EEEEEECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEE
ALANIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLL
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEE
MGIMLTGYPEKFGALVCDVPLLDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQN
EEEEEECCCHHHCCEEECCCHHCCCEEEEEEECHHHHHHCCCCCCCCCCHHHHCCCCCCC
ISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYYENIEGGHAGAADNAQIA
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHH
FKSALSFAFLWRMLAG
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]