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The map label for this gene is echA2 [H]

Identifier: 121636370

GI number: 121636370

Start: 576704

End: 577618

Strand: Reverse

Name: echA2 [H]

Synonym: BCG_0495c

Alternate gene names: 121636370

Gene position: 577618-576704 (Counterclockwise)

Preceding gene: 121636371

Following gene: 121636369

Centisome position: 13.2

GC content: 66.23

Gene sequence:

>915_bases
ATGCCGACACCCGATTTCCAGACGCTGCTGTACACGACGGCCGGGCCGGTGGCCACCATCACGCTCAACCGCCCGGAACA
GCTCAACACCATCGTCCCGCCCATGCCCGACGAGATCGAGGCCGCTATCGGGTTGGCCGAGCGCGACCAGGACATCAAGG
TCATCGTGCTGCGCGGTGCCGGCCGCGCCTTCTCCGGCGGTTACGACTTCGGCGGCGGCTTCCAGCATTGGGGCGATGCC
ATGATGACCGACGGCCGATGGGATCCGGGCAAGGATTTCGCCATGGTCACCGCGCGGGAGACCGGACCGACGCAGAAATT
CATGGCCATCTGGCGGGCGTCCAAACCGGTGATCGCGCAAGTGCATGGTTGGTGCGTCGGCGGGGCCAGCGACTACGCGC
TGTGTGCCGACATTGTGATCGCCAGCGAGGACGCCGTGATCGGGACCCCGTATAGCCGCATGTGGGGAGCCTATTTGACC
GGGATGTGGCTGTATCGACTCAGCCTTGCCAAGGTCAAATGGCACTCGCTGACGGGCCGGCCGCTGACCGGTGTGCAGGC
CGCCGAAGCCGAGCTGATCAACGAGGCGGTGCCGTTCGAGCGGCTCGAGGCTCGCGTCGCCGAGATCGCCACCGAGCTGG
CACGAATCCCGTTGTCACAGTTGCAAGCCCAGAAACTGATCGTCAACCAGGCCTACGAGAACATGGGCCTGGCCTCCACC
CAGCTGCTGGGCGGCATTCTCGACGGGCTGATGCGCAACACCCCCGACGCGCTCGAGTTCATCCGGACCGCCCAAACCCA
GGGTGTGCGAGCCGCGGTCGAGCGCCGCGACGGCCCGTTCGGCGACTACAGCCAAGCCCCACCGGAACTGCGACCCGACC
CCACGCACGTCATCACTCCTGATGGGAGCATGTAG

Upstream 100 bases:

>100_bases
AACCCTGCGCCCGGGTTGGCCGAAGCTTCGAACCAGCGGCGTTATCACGATCCCCGCTGAGACACTTGACCCTTAGGGCC
GAAGTGACTTCGCTGCTGCT

Downstream 100 bases:

>100_bases
TGAGACAGACTATGAACCTTGTCGTCTGCCCGCCTCGAGGCGCCCCAAAAGTGCTACGGTAACCACTATGTCTCGGCTGA
GTTCCATCCTGCGTGCCGGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPTPDFQTLLYTTAGPVATITLNRPEQLNTIVPPMPDEIEAAIGLAERDQDIKVIVLRGAGRAFSGGYDFGGGFQHWGDA
MMTDGRWDPGKDFAMVTARETGPTQKFMAIWRASKPVIAQVHGWCVGGASDYALCADIVIASEDAVIGTPYSRMWGAYLT
GMWLYRLSLAKVKWHSLTGRPLTGVQAAEAELINEAVPFERLEARVAEIATELARIPLSQLQAQKLIVNQAYENMGLAST
QLLGGILDGLMRNTPDALEFIRTAQTQGVRAAVERRDGPFGDYSQAPPELRPDPTHVITPDGSM

Sequences:

>Translated_304_residues
MPTPDFQTLLYTTAGPVATITLNRPEQLNTIVPPMPDEIEAAIGLAERDQDIKVIVLRGAGRAFSGGYDFGGGFQHWGDA
MMTDGRWDPGKDFAMVTARETGPTQKFMAIWRASKPVIAQVHGWCVGGASDYALCADIVIASEDAVIGTPYSRMWGAYLT
GMWLYRLSLAKVKWHSLTGRPLTGVQAAEAELINEAVPFERLEARVAEIATELARIPLSQLQAQKLIVNQAYENMGLAST
QLLGGILDGLMRNTPDALEFIRTAQTQGVRAAVERRDGPFGDYSQAPPELRPDPTHVITPDGSM
>Mature_303_residues
PTPDFQTLLYTTAGPVATITLNRPEQLNTIVPPMPDEIEAAIGLAERDQDIKVIVLRGAGRAFSGGYDFGGGFQHWGDAM
MTDGRWDPGKDFAMVTARETGPTQKFMAIWRASKPVIAQVHGWCVGGASDYALCADIVIASEDAVIGTPYSRMWGAYLTG
MWLYRLSLAKVKWHSLTGRPLTGVQAAEAELINEAVPFERLEARVAEIATELARIPLSQLQAQKLIVNQAYENMGLASTQ
LLGGILDGLMRNTPDALEFIRTAQTQGVRAAVERRDGPFGDYSQAPPELRPDPTHVITPDGSM

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=27.5471698113208, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787660, Length=224, Percent_Identity=31.6964285714286, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=224, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357171, Length=215, Percent_Identity=27.4418604651163, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=232, Percent_Identity=26.2931034482759, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 33086; Mature: 32955

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPDFQTLLYTTAGPVATITLNRPEQLNTIVPPMPDEIEAAIGLAERDQDIKVIVLRGA
CCCCCCCEEEEECCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
GRAFSGGYDFGGGFQHWGDAMMTDGRWDPGKDFAMVTARETGPTQKFMAIWRASKPVIAQ
CCCCCCCCCCCCCHHHHCCHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEE
VHGWCVGGASDYALCADIVIASEDAVIGTPYSRMWGAYLTGMWLYRLSLAKVKWHSLTGR
ECCEEECCCCCHHHHHHEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
PLTGVQAAEAELINEAVPFERLEARVAEIATELARIPLSQLQAQKLIVNQAYENMGLAST
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHH
QLLGGILDGLMRNTPDALEFIRTAQTQGVRAAVERRDGPFGDYSQAPPELRPDPTHVITP
HHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECC
DGSM
CCCC
>Mature Secondary Structure 
PTPDFQTLLYTTAGPVATITLNRPEQLNTIVPPMPDEIEAAIGLAERDQDIKVIVLRGA
CCCCCCEEEEECCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
GRAFSGGYDFGGGFQHWGDAMMTDGRWDPGKDFAMVTARETGPTQKFMAIWRASKPVIAQ
CCCCCCCCCCCCCHHHHCCHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEE
VHGWCVGGASDYALCADIVIASEDAVIGTPYSRMWGAYLTGMWLYRLSLAKVKWHSLTGR
ECCEEECCCCCHHHHHHEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
PLTGVQAAEAELINEAVPFERLEARVAEIATELARIPLSQLQAQKLIVNQAYENMGLAST
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHH
QLLGGILDGLMRNTPDALEFIRTAQTQGVRAAVERRDGPFGDYSQAPPELRPDPTHVITP
HHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECC
DGSM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]