Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 121610346

GI number: 121610346

Start: 3784544

End: 3785290

Strand: Direct

Name: rlmB [H]

Synonym: Veis_3414

Alternate gene names: 121610346

Gene position: 3784544-3785290 (Clockwise)

Preceding gene: 121610345

Following gene: 121610353

Centisome position: 67.98

GC content: 69.75

Gene sequence:

>747_bases
ATGTCCAGCCCCAAAGTCCTGTTCGGCTTTCACGCCGTGGGCGTGCGTCTGAAGACCGCGCCCGAATCGGTCATCGAGAT
CTACCACGAGGCCACGCGGCGCGACGCGCGCATGCGCCAGTTTCTCGAACGCGCCCGCGCGGCCGGCGCCCGCCTGATCG
AGGCTGACGGCATGCGCATCGCCAAGCTGGCCGGCAGCCATGGCCACCAGGGCGTGGCCGCGCGCGTGCAGCCCGTGGCG
CCGATCAGTTCGCTCGACGAACTGCTGGAAAAGCTCCAGGCCGCAGGCAGCGGACCGCCCTTGCTGCTGGTGCTCGACGG
CGTGACCGACCCGCACAACCTCGGGGCCTGCCTGCGTGTGGCCGACGGGGCCGGGGCGCATGCCGTGATTGCGCCCAAGG
ACCATGCCGTGGGCATCAACGCCACCGTGGCCAAGGTGGCCAGCGGGGCGGCCGAGACCATGCCGTACTTCATGGTGACC
AACCTCGCGCGCACGCTCGATGAACTCAAGGAGCGCAGCATCTGGTGCATAGGCACCTGCGACGACGCGCCCGGCACCTT
GTACCAACTCGACCTCAAAGGCCCCGTGGCACTGGTGCTGGGCGCCGAGGGCAGCGGCATGCGCCAGCGCACCCGCAAGA
CCTGCGACGAACTGGCGCGCATCCCGATGCAGGGCGCGGTGGAGAGCCTGAACGTCTCGGTGGCCAGCGGCATATGCCTG
TACGAGGCCCTGCGCCAACGCGCCTAG

Upstream 100 bases:

>100_bases
GCGCTGCGCTTGGCCCATGATGCAAGCAGATCATTCATCGGAAGCTATGTTTTCGGTAGCAACATGACGCTGCCATCGGC
CGCCAGCGGATAATCGCCCG

Downstream 100 bases:

>100_bases
TGTCGCGTCACCGATCATCTGACCGGTGACGCGACACTAGTCTGCGCTCCCTTGAGCGCCGCCATCACCGGCCTATGGTC
TGCGCAACGTGGCGCGCTGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSSPKVLFGFHAVGVRLKTAPESVIEIYHEATRRDARMRQFLERARAAGARLIEADGMRIAKLAGSHGHQGVAARVQPVA
PISSLDELLEKLQAAGSGPPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVT
NLARTLDELKERSIWCIGTCDDAPGTLYQLDLKGPVALVLGAEGSGMRQRTRKTCDELARIPMQGAVESLNVSVASGICL
YEALRQRA

Sequences:

>Translated_248_residues
MSSPKVLFGFHAVGVRLKTAPESVIEIYHEATRRDARMRQFLERARAAGARLIEADGMRIAKLAGSHGHQGVAARVQPVA
PISSLDELLEKLQAAGSGPPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVT
NLARTLDELKERSIWCIGTCDDAPGTLYQLDLKGPVALVLGAEGSGMRQRTRKTCDELARIPMQGAVESLNVSVASGICL
YEALRQRA
>Mature_247_residues
SSPKVLFGFHAVGVRLKTAPESVIEIYHEATRRDARMRQFLERARAAGARLIEADGMRIAKLAGSHGHQGVAARVQPVAP
ISSLDELLEKLQAAGSGPPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVTN
LARTLDELKERSIWCIGTCDDAPGTLYQLDLKGPVALVLGAEGSGMRQRTRKTCDELARIPMQGAVESLNVSVASGICLY
EALRQRA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=267, Percent_Identity=28.8389513108614, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790623, Length=246, Percent_Identity=48.780487804878, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=35.0649350649351, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788935, Length=242, Percent_Identity=23.9669421487603, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=31.5151515151515, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26266; Mature: 26134

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPKVLFGFHAVGVRLKTAPESVIEIYHEATRRDARMRQFLERARAAGARLIEADGMRI
CCCCCEEEEEEEECEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
AKLAGSHGHQGVAARVQPVAPISSLDELLEKLQAAGSGPPLLLVLDGVTDPHNLGACLRV
EECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEE
ADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVTNLARTLDELKERSIWCIGTC
CCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
DDAPGTLYQLDLKGPVALVLGAEGSGMRQRTRKTCDELARIPMQGAVESLNVSVASGICL
CCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YEALRQRA
HHHHHHCC
>Mature Secondary Structure 
SSPKVLFGFHAVGVRLKTAPESVIEIYHEATRRDARMRQFLERARAAGARLIEADGMRI
CCCCEEEEEEEECEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
AKLAGSHGHQGVAARVQPVAPISSLDELLEKLQAAGSGPPLLLVLDGVTDPHNLGACLRV
EECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEE
ADGAGAHAVIAPKDHAVGINATVAKVASGAAETMPYFMVTNLARTLDELKERSIWCIGTC
CCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
DDAPGTLYQLDLKGPVALVLGAEGSGMRQRTRKTCDELARIPMQGAVESLNVSVASGICL
CCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YEALRQRA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]