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The map label for this gene is ygbJ [C]

Identifier: 121610320

GI number: 121610320

Start: 3758426

End: 3759346

Strand: Direct

Name: ygbJ [C]

Synonym: Veis_3388

Alternate gene names: 121610320

Gene position: 3758426-3759346 (Clockwise)

Preceding gene: 121610319

Following gene: 121610322

Centisome position: 67.52

GC content: 66.99

Gene sequence:

>921_bases
ATGAGAAAAGCAGGAATCGTCGGCTTGGGCGCCATGGGCGCAGGCATCGCACAGACGCTGCGTGCCAACGGCTATGAAGT
GCATGTGTGCGATCTGCGGCCGGGCGTGGCGACGGCCTTCGCTGCGCAGGGCGGCGTCGCCTGCAACAACCCCGCCGAAG
TCGCGGCGGCTTGCGCGGTGGTGGTGAGCGTCGTGGTCAACGCGGCGCAGACCGAGGCCGTGCTGTTCGGCGCAGGTGGC
GCAGCCGCAGCCATGAAGCCCGGCAGCACCTTCGTCATGTGTTCCACCGTCGATCCGCACTGGTCGGCCGCACTCGAAGA
TCGGTTGGGCCAGGCCGGCATTCATTACATCGACGCGCCGATCTCCGGCGGCGCGGCCAAGGCCGCGAGCGGGCAGATGA
CGGTGATGTCGTCCGCCAAGCCGCAAGCCTATGCGCAGGCGGGTGCGGTGCTCGACGCGATGGCCGGCAAGGTGTATCGC
CTGGGCGACCGCGCGGGCGCGGGCAGCAAGGTCAAGATCATCAACCAGTTGCTGGCCGGCGTGCACATCGCGGCGGCGGC
CGAGGCGATGGCGCTGGGCCTGCGCGAGGGCGTGGCACTGGACGCGCTCTACGAGGTGATCACGCACAGTGCGGGCAATA
GCTGGATGTTCGAGAACCGCATGGCCCATGTGCTGGCGGGCGATTACACGCCGCTGTCGGCGGTCGACATCTTCGTCAAG
GACCTGGGCTTGGTGCTCGATGCCGCGCGTGCGAGCAAGTTTCCGCTGCCGCTCGCGTCCACCGCGCACCAGATGTTCAT
GCAGGCGTCGTCCGCCGGCTTCGCCAACGAAGACGACAGCGCGGTGATCAAGATCTTCCCCGGCATCGAGTTGCCCAAAG
GAAAGGGAAAGGGAAAGGGAAAGGGAAAGGGAAAGGCCTGA

Upstream 100 bases:

>100_bases
TAGTGATTTCATCGTGTCAGTCGGCGCCCCGGGATGCGGCAAGCCGACGAGGTGAGCCAACGAGTCTTTTGGAACCTCCT
CCGGAAAGGCAGCCAGCCCC

Downstream 100 bases:

>100_bases
GCACCAGGCCCGGCGGCATCGCCGGCGATGGCGCCGGAGCCACCGATCGGGCCAACCCACCTGGCCAGCTCGCTGGGTGC
GCGTCGGCATGCGCGTGGCG

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRKAGIVGLGAMGAGIAQTLRANGYEVHVCDLRPGVATAFAAQGGVACNNPAEVAAACAVVVSVVVNAAQTEAVLFGAGG
AAAAMKPGSTFVMCSTVDPHWSAALEDRLGQAGIHYIDAPISGGAAKAASGQMTVMSSAKPQAYAQAGAVLDAMAGKVYR
LGDRAGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVALDALYEVITHSAGNSWMFENRMAHVLAGDYTPLSAVDIFVK
DLGLVLDAARASKFPLPLASTAHQMFMQASSAGFANEDDSAVIKIFPGIELPKGKGKGKGKGKGKA

Sequences:

>Translated_306_residues
MRKAGIVGLGAMGAGIAQTLRANGYEVHVCDLRPGVATAFAAQGGVACNNPAEVAAACAVVVSVVVNAAQTEAVLFGAGG
AAAAMKPGSTFVMCSTVDPHWSAALEDRLGQAGIHYIDAPISGGAAKAASGQMTVMSSAKPQAYAQAGAVLDAMAGKVYR
LGDRAGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVALDALYEVITHSAGNSWMFENRMAHVLAGDYTPLSAVDIFVK
DLGLVLDAARASKFPLPLASTAHQMFMQASSAGFANEDDSAVIKIFPGIELPKGKGKGKGKGKGKA
>Mature_306_residues
MRKAGIVGLGAMGAGIAQTLRANGYEVHVCDLRPGVATAFAAQGGVACNNPAEVAAACAVVVSVVVNAAQTEAVLFGAGG
AAAAMKPGSTFVMCSTVDPHWSAALEDRLGQAGIHYIDAPISGGAAKAASGQMTVMSSAKPQAYAQAGAVLDAMAGKVYR
LGDRAGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVALDALYEVITHSAGNSWMFENRMAHVLAGDYTPLSAVDIFVK
DLGLVLDAARASKFPLPLASTAHQMFMQASSAGFANEDDSAVIKIFPGIELPKGKGKGKGKGKGKA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=30.4498269896194, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=24.5614035087719, Blast_Score=111, Evalue=6e-25,
Organism=Escherichia coli, GI1789092, Length=294, Percent_Identity=53.7414965986395, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=29.2857142857143, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=25.886524822695, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=26.4285714285714, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=283, Percent_Identity=30.7420494699647, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=29.4520547945205, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=29.4520547945205, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=25.7142857142857, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30892; Mature: 30892

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAGIVGLGAMGAGIAQTLRANGYEVHVCDLRPGVATAFAAQGGVACNNPAEVAAACAV
CCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHH
VVSVVVNAAQTEAVLFGAGGAAAAMKPGSTFVMCSTVDPHWSAALEDRLGQAGIHYIDAP
HHHHHHCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECC
ISGGAAKAASGQMTVMSSAKPQAYAQAGAVLDAMAGKVYRLGDRAGAGSKVKIINQLLAG
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHH
VHIAAAAEAMALGLREGVALDALYEVITHSAGNSWMFENRMAHVLAGDYTPLSAVDIFVK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHH
DLGLVLDAARASKFPLPLASTAHQMFMQASSAGFANEDDSAVIKIFPGIELPKGKGKGKG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
KGKGKA
CCCCCC
>Mature Secondary Structure
MRKAGIVGLGAMGAGIAQTLRANGYEVHVCDLRPGVATAFAAQGGVACNNPAEVAAACAV
CCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHH
VVSVVVNAAQTEAVLFGAGGAAAAMKPGSTFVMCSTVDPHWSAALEDRLGQAGIHYIDAP
HHHHHHCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECC
ISGGAAKAASGQMTVMSSAKPQAYAQAGAVLDAMAGKVYRLGDRAGAGSKVKIINQLLAG
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHH
VHIAAAAEAMALGLREGVALDALYEVITHSAGNSWMFENRMAHVLAGDYTPLSAVDIFVK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHH
DLGLVLDAARASKFPLPLASTAHQMFMQASSAGFANEDDSAVIKIFPGIELPKGKGKGKG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
KGKGKA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]