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The map label for this gene is phnD [H]

Identifier: 121609911

GI number: 121609911

Start: 3316157

End: 3317101

Strand: Direct

Name: phnD [H]

Synonym: Veis_2965

Alternate gene names: 121609911

Gene position: 3316157-3317101 (Clockwise)

Preceding gene: 121609910

Following gene: 121609912

Centisome position: 59.57

GC content: 60.63

Gene sequence:

>945_bases
ATGTTCAAGCAACTGGGCGCCGCCTTGGCCGCCACCCTGACCATGGGTCTGTCTGCGGCCCAGGACATCACTTTCAGCTT
CATTTCCACCGAAGCCTCGCAAAACATCAAAGCCGACTGGCAGCCCCTGCTGGACGACATGGCGCGGCAGACCGGCCTCA
AAGTCCACGCCTTTTTCTCCCCCGACTACGCCGGCATCATCGAAGGCATGCGCTTTGGCAAGGTGCAGGCGGCCTGGCTG
GGCAACAAATCGGCAATGGAGGCCGTGGACCGCGCCAACGGCGAGGTGTTCGCCCAGATGGTCAACGCCGATGGCACGCA
GGGCTACTACTCGCACCTGATCGTGCACAAAGACAGCCCCTGCAACACGCTGGACGACGTGCTGAAAAACGCCCGCTCGC
TCAGCTTTGGCAATGGCGACCCGAACTCCACCTCGGGCTATCTGGTGCCGGGCTACTACGCCTTCGCGCTGAACAAGTTC
GACCCCAAGACGCATTTCAAGATCGTGCGCAGCGCCAACCACGAAACCAACGCCCTGGCCGTGGTCAACAAGCAGGTCGA
TGTGGCCACGAACAACAGCGAGAACCTGGAAAAGATCAAAGAGCGCTATCCCGAGAAGTTCAAGGACATCAAAGTCATCT
GGACCTCGCCGCTGATCGCGCTCGACCCGCTGGTCATGCACAAGGGCCTGGCGCCGGCCACCAAGGCCAAGATCAAGGAC
TTTTTCTACAACTACGCCAAGACCGATGCGCGCGAGAAAGAGATCGTGATGAAGCTGTCCAAGCTGTCGGGCTTCAAGCC
GTCGAGCAACGCGCAGCTCACACCCATCCGCCAGCTGACCCTGTTCGGCAAGCGCAACAAGATCGAAGCCGACACCACGC
TGACCGAGGCCGACAAGAAAACCCGGCTGGCCGAGATCGACCAGCAACTGGCCGCGTTGCAGTGA

Upstream 100 bases:

>100_bases
CCCCATCGCCGATCACGCCGCTGCCGCAGTTCGCGCCGGCGATGGCCCGGGCCGCCTGACCGGCCCGTTCACTTCTTCCA
CCCGTTTTTGGAGTTCCCTC

Downstream 100 bases:

>100_bases
AACACCCTCCCGAGACATTTTCGACACCCACAGGTACCCCCATGTCCTCTGCCCCGAACCCCTTGGCGCTGGCCCAGACC
GCCATCCCCCGGCACAGCCT

Product: phosphonate ABC transporter periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MFKQLGAALAATLTMGLSAAQDITFSFISTEASQNIKADWQPLLDDMARQTGLKVHAFFSPDYAGIIEGMRFGKVQAAWL
GNKSAMEAVDRANGEVFAQMVNADGTQGYYSHLIVHKDSPCNTLDDVLKNARSLSFGNGDPNSTSGYLVPGYYAFALNKF
DPKTHFKIVRSANHETNALAVVNKQVDVATNNSENLEKIKERYPEKFKDIKVIWTSPLIALDPLVMHKGLAPATKAKIKD
FFYNYAKTDAREKEIVMKLSKLSGFKPSSNAQLTPIRQLTLFGKRNKIEADTTLTEADKKTRLAEIDQQLAALQ

Sequences:

>Translated_314_residues
MFKQLGAALAATLTMGLSAAQDITFSFISTEASQNIKADWQPLLDDMARQTGLKVHAFFSPDYAGIIEGMRFGKVQAAWL
GNKSAMEAVDRANGEVFAQMVNADGTQGYYSHLIVHKDSPCNTLDDVLKNARSLSFGNGDPNSTSGYLVPGYYAFALNKF
DPKTHFKIVRSANHETNALAVVNKQVDVATNNSENLEKIKERYPEKFKDIKVIWTSPLIALDPLVMHKGLAPATKAKIKD
FFYNYAKTDAREKEIVMKLSKLSGFKPSSNAQLTPIRQLTLFGKRNKIEADTTLTEADKKTRLAEIDQQLAALQ
>Mature_314_residues
MFKQLGAALAATLTMGLSAAQDITFSFISTEASQNIKADWQPLLDDMARQTGLKVHAFFSPDYAGIIEGMRFGKVQAAWL
GNKSAMEAVDRANGEVFAQMVNADGTQGYYSHLIVHKDSPCNTLDDVLKNARSLSFGNGDPNSTSGYLVPGYYAFALNKF
DPKTHFKIVRSANHETNALAVVNKQVDVATNNSENLEKIKERYPEKFKDIKVIWTSPLIALDPLVMHKGLAPATKAKIKD
FFYNYAKTDAREKEIVMKLSKLSGFKPSSNAQLTPIRQLTLFGKRNKIEADTTLTEADKKTRLAEIDQQLAALQ

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=288, Percent_Identity=57.9861111111111, Blast_Score=334, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 34732; Mature: 34732

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQLGAALAATLTMGLSAAQDITFSFISTEASQNIKADWQPLLDDMARQTGLKVHAFFS
CCHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEC
PDYAGIIEGMRFGKVQAAWLGNKSAMEAVDRANGEVFAQMVNADGTQGYYSHLIVHKDSP
CCHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCHHEEHHHHCCCCCCCHHEEEEEECCCC
CNTLDDVLKNARSLSFGNGDPNSTSGYLVPGYYAFALNKFDPKTHFKIVRSANHETNALA
CCHHHHHHHHHHCCCCCCCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHCCCCCCCEEE
VVNKQVDVATNNSENLEKIKERYPEKFKDIKVIWTSPLIALDPLVMHKGLAPATKAKIKD
EEECEEEEECCCHHHHHHHHHHCCHHHCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHH
FFYNYAKTDAREKEIVMKLSKLSGFKPSSNAQLTPIRQLTLFGKRNKIEADTTLTEADKK
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEECCCCCCCCCCHHHHHHHH
TRLAEIDQQLAALQ
HHHHHHHHHHHHCC
>Mature Secondary Structure
MFKQLGAALAATLTMGLSAAQDITFSFISTEASQNIKADWQPLLDDMARQTGLKVHAFFS
CCHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEC
PDYAGIIEGMRFGKVQAAWLGNKSAMEAVDRANGEVFAQMVNADGTQGYYSHLIVHKDSP
CCHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCHHEEHHHHCCCCCCCHHEEEEEECCCC
CNTLDDVLKNARSLSFGNGDPNSTSGYLVPGYYAFALNKFDPKTHFKIVRSANHETNALA
CCHHHHHHHHHHCCCCCCCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHCCCCCCCEEE
VVNKQVDVATNNSENLEKIKERYPEKFKDIKVIWTSPLIALDPLVMHKGLAPATKAKIKD
EEECEEEEECCCHHHHHHHHHHCCHHHCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHH
FFYNYAKTDAREKEIVMKLSKLSGFKPSSNAQLTPIRQLTLFGKRNKIEADTTLTEADKK
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEECCCCCCCCCCHHHHHHHH
TRLAEIDQQLAALQ
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]