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The map label for this gene is ycdT [H]

Identifier: 121609900

GI number: 121609900

Start: 3305661

End: 3307499

Strand: Direct

Name: ycdT [H]

Synonym: Veis_2954

Alternate gene names: 121609900

Gene position: 3305661-3307499 (Clockwise)

Preceding gene: 121609895

Following gene: 121609903

Centisome position: 59.38

GC content: 63.46

Gene sequence:

>1839_bases
ATGGGCAAACGATGGTGGTGGGTGGCGCTGGTGCTGGCGCTATGTCCGTTTTGGACCTCTCAGGCGGGGGCTGGCTTGGT
CCGCCTGGTGCCCGGCCAGGGCAGTTACGACCTGGGCGCCATGGCCGATGTGTTGGAGGACGTGCCGGGCCGGTTGCGGC
TCGATGATGTGCTGGCCGGCGGCAGTGCTGCGTCTGCCTTCAGTTCCCATACCGGCCGCCTGTCCAGTTTCGGGTTTACC
AGGTCGACTTTCTGGTTCCGCGTCGAGATCGACAATCCCGCGCCTGCGCCGAGCGACATGCTGTTGGTGCTGCGCACCCC
TTGGCTCGACTCCGTGCGCTGGTTCCGCCCGGACGGGCAGGGTGGCTATGTCGAACGACTGCTGGGCGATCACCTGCCGT
TCAAGGCCCGCTCCTATGCCACGCCGCAGTTTCTGATCGACGTGCCGGTGCTGCCCGGCAAGCACAGCTACTACCTGCGT
CTGGCCAGTGCCGGGGCCTTCATGACGCCCATCGAGCTGTGGAGCCGCCAGGCTTTCCATGACAACGATCGCCTGTGGGC
GGCCTACTACGGGATGTTCTACGGCATCCTGTGCATCATGGTGCTCTACAACGGCTGCATCTGGGCCTCCACGCGCGACC
GCAGTTACTTCGTTTACTGCCTGTACCTGGCGGTGTTTTTCCTGATGAACTTTTCGTACAACGGCTTTGCGTTCCAGTAT
TTCTGGCCTGAGTCGCCCCGCTGGAGCAATTGGAGCCAGACGCATTGGATCTTCGCGTTCCAGGGGCTGGCGGTATTGTT
CACGATGAATTTCCTGGAGTCGAAAACCCGCCTGCCGCGCATGCACCGGGTCTTGCAAGCCTTCCTGGTGGTGCTGGTCG
GCAGTTGGATCGCGGTCACCGTCAGCGGCGATATCGTGGCCTACCACGCGGTGCCGATTTATTTTATTTTTGGCTGCGCG
CCGCTGATTCTGGCCGCAGGCTTTCTGGCCTTGAGGCCCGGTTATCGGGCGGCGCGCTTTTTCGTGCTGGCCTTTGTCGC
CAGCATCACCGGGAGTTTTTTCACGGCGCTGACCGTGAGCGGCCTGATTCCCTATACCTTTGCCAATTTCCACGCCTCCG
AGTTCGGCCTGCTGGCGGACATGGTGCTGCTCTCGCAGGCCCTGGCCGATCGCGTCAAGCTGTTGAGCGAACAGCGCGAT
GCGGCCGAGTTGCGCGAAATCGAGCAAAAGATCGCCACCACGGCCTTGCTGAAACAGGCCAACGAAGACCTGGAGCGAAT
GGTGCTGGAGCGCACTTCGGAGCTGGCCAGGGCGCGCGACGAAGCCGAGCACTTTGCCCGCATCGACATGCTGACGGGCG
TGGCCAATCGCCGTTACTTCGAGGAAGTCGCGACCATGGAATTTGCCCGGACGCAGCGTGGCCAGCAAGCCTTGTCGGTC
ATCCTGATCGATATCGATCTGTTCAAGCAGGTCAACGACCGCTATGGCCATGCGGCCGGTGATGCGGTGATCCGGGCCGT
CGCCCGCCTGGCCAAGGAGTCGGTGCGGGAGTCCGATTTCGTGGCCCGCATCGGTGGCGAGGAGTTCTCCGTCCTGTTGC
CCGGCATAGGGCAGGCGCCGGCCTTTGCCACCGCCGAGCGTTTGCGTGAGCGGATTGCCGCCTGTGTGGTCGAGCATGAC
GGACATTCGCTGGGCTTTACCGCCAGCTTTGGCGTCAGCGAGCAGGTTGCCGACGACCCCGGGTTCAGCAGCCTGTTGCG
CCGGGCAGACCAGGCCCTGTACGCTGCCAAGCAGGCGGGGCGCAACCAGGTGGTGGTGCAGGCGCTCGAGTGGGGGTGA

Upstream 100 bases:

>100_bases
GAACGGGGGCATTTTGAACGACTTTTCAGGCGTGGGGCCTGTTCGGGATCGATCGGGTGCGGAACTCCGGGGCGCGGCGC
AACAAGGCAGGTCGATGCAG

Downstream 100 bases:

>100_bases
GTGCGCTGAAAAAACCGCAGGCCAGGCAGCGCCGGGCTTGGCACGGGGCACAGCGCCACAAGCCGGCGCTGTGCCACGCC
GGCGCGGAGCGTGTCACAGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MGKRWWWVALVLALCPFWTSQAGAGLVRLVPGQGSYDLGAMADVLEDVPGRLRLDDVLAGGSAASAFSSHTGRLSSFGFT
RSTFWFRVEIDNPAPAPSDMLLVLRTPWLDSVRWFRPDGQGGYVERLLGDHLPFKARSYATPQFLIDVPVLPGKHSYYLR
LASAGAFMTPIELWSRQAFHDNDRLWAAYYGMFYGILCIMVLYNGCIWASTRDRSYFVYCLYLAVFFLMNFSYNGFAFQY
FWPESPRWSNWSQTHWIFAFQGLAVLFTMNFLESKTRLPRMHRVLQAFLVVLVGSWIAVTVSGDIVAYHAVPIYFIFGCA
PLILAAGFLALRPGYRAARFFVLAFVASITGSFFTALTVSGLIPYTFANFHASEFGLLADMVLLSQALADRVKLLSEQRD
AAELREIEQKIATTALLKQANEDLERMVLERTSELARARDEAEHFARIDMLTGVANRRYFEEVATMEFARTQRGQQALSV
ILIDIDLFKQVNDRYGHAAGDAVIRAVARLAKESVRESDFVARIGGEEFSVLLPGIGQAPAFATAERLRERIAACVVEHD
GHSLGFTASFGVSEQVADDPGFSSLLRRADQALYAAKQAGRNQVVVQALEWG

Sequences:

>Translated_612_residues
MGKRWWWVALVLALCPFWTSQAGAGLVRLVPGQGSYDLGAMADVLEDVPGRLRLDDVLAGGSAASAFSSHTGRLSSFGFT
RSTFWFRVEIDNPAPAPSDMLLVLRTPWLDSVRWFRPDGQGGYVERLLGDHLPFKARSYATPQFLIDVPVLPGKHSYYLR
LASAGAFMTPIELWSRQAFHDNDRLWAAYYGMFYGILCIMVLYNGCIWASTRDRSYFVYCLYLAVFFLMNFSYNGFAFQY
FWPESPRWSNWSQTHWIFAFQGLAVLFTMNFLESKTRLPRMHRVLQAFLVVLVGSWIAVTVSGDIVAYHAVPIYFIFGCA
PLILAAGFLALRPGYRAARFFVLAFVASITGSFFTALTVSGLIPYTFANFHASEFGLLADMVLLSQALADRVKLLSEQRD
AAELREIEQKIATTALLKQANEDLERMVLERTSELARARDEAEHFARIDMLTGVANRRYFEEVATMEFARTQRGQQALSV
ILIDIDLFKQVNDRYGHAAGDAVIRAVARLAKESVRESDFVARIGGEEFSVLLPGIGQAPAFATAERLRERIAACVVEHD
GHSLGFTASFGVSEQVADDPGFSSLLRRADQALYAAKQAGRNQVVVQALEWG
>Mature_611_residues
GKRWWWVALVLALCPFWTSQAGAGLVRLVPGQGSYDLGAMADVLEDVPGRLRLDDVLAGGSAASAFSSHTGRLSSFGFTR
STFWFRVEIDNPAPAPSDMLLVLRTPWLDSVRWFRPDGQGGYVERLLGDHLPFKARSYATPQFLIDVPVLPGKHSYYLRL
ASAGAFMTPIELWSRQAFHDNDRLWAAYYGMFYGILCIMVLYNGCIWASTRDRSYFVYCLYLAVFFLMNFSYNGFAFQYF
WPESPRWSNWSQTHWIFAFQGLAVLFTMNFLESKTRLPRMHRVLQAFLVVLVGSWIAVTVSGDIVAYHAVPIYFIFGCAP
LILAAGFLALRPGYRAARFFVLAFVASITGSFFTALTVSGLIPYTFANFHASEFGLLADMVLLSQALADRVKLLSEQRDA
AELREIEQKIATTALLKQANEDLERMVLERTSELARARDEAEHFARIDMLTGVANRRYFEEVATMEFARTQRGQQALSVI
LIDIDLFKQVNDRYGHAAGDAVIRAVARLAKESVRESDFVARIGGEEFSVLLPGIGQAPAFATAERLRERIAACVVEHDG
HSLGFTASFGVSEQVADDPGFSSLLRRADQALYAAKQAGRNQVVVQALEWG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=40, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=35.9756097560976, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081881, Length=188, Percent_Identity=36.7021276595745, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=32.4022346368715, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=41.1392405063291, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=33.879781420765, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=35.1515151515151, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=30.1075268817204, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=32.7160493827161, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=32.4022346368715, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=150, Percent_Identity=28.6666666666667, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=150, Percent_Identity=30, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=31.25, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 68463; Mature: 68332

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRWWWVALVLALCPFWTSQAGAGLVRLVPGQGSYDLGAMADVLEDVPGRLRLDDVLAG
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEHHHHHCC
GSAASAFSSHTGRLSSFGFTRSTFWFRVEIDNPAPAPSDMLLVLRTPWLDSVRWFRPDGQ
CCHHHHHHHCCCCHHHCCCEECEEEEEEEECCCCCCCCCEEEEEECCCCCCCCEECCCCC
GGYVERLLGDHLPFKARSYATPQFLIDVPVLPGKHSYYLRLASAGAFMTPIELWSRQAFH
CCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCC
DNDRLWAAYYGMFYGILCIMVLYNGCIWASTRDRSYFVYCLYLAVFFLMNFSYNGFAFQY
CCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
FWPESPRWSNWSQTHWIFAFQGLAVLFTMNFLESKTRLPRMHRVLQAFLVVLVGSWIAVT
ECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEE
VSGDIVAYHAVPIYFIFGCAPLILAAGFLALRPGYRAARFFVLAFVASITGSFFTALTVS
EECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIPYTFANFHASEFGLLADMVLLSQALADRVKLLSEQRDAAELREIEQKIATTALLKQA
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NEDLERMVLERTSELARARDEAEHFARIDMLTGVANRRYFEEVATMEFARTQRGQQALSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIDIDLFKQVNDRYGHAAGDAVIRAVARLAKESVRESDFVARIGGEEFSVLLPGIGQAP
HEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
AFATAERLRERIAACVVEHDGHSLGFTASFGVSEQVADDPGFSSLLRRADQALYAAKQAG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC
RNQVVVQALEWG
CCEEEEEEECCC
>Mature Secondary Structure 
GKRWWWVALVLALCPFWTSQAGAGLVRLVPGQGSYDLGAMADVLEDVPGRLRLDDVLAG
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEHHHHHCC
GSAASAFSSHTGRLSSFGFTRSTFWFRVEIDNPAPAPSDMLLVLRTPWLDSVRWFRPDGQ
CCHHHHHHHCCCCHHHCCCEECEEEEEEEECCCCCCCCCEEEEEECCCCCCCCEECCCCC
GGYVERLLGDHLPFKARSYATPQFLIDVPVLPGKHSYYLRLASAGAFMTPIELWSRQAFH
CCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCC
DNDRLWAAYYGMFYGILCIMVLYNGCIWASTRDRSYFVYCLYLAVFFLMNFSYNGFAFQY
CCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
FWPESPRWSNWSQTHWIFAFQGLAVLFTMNFLESKTRLPRMHRVLQAFLVVLVGSWIAVT
ECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEE
VSGDIVAYHAVPIYFIFGCAPLILAAGFLALRPGYRAARFFVLAFVASITGSFFTALTVS
EECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIPYTFANFHASEFGLLADMVLLSQALADRVKLLSEQRDAAELREIEQKIATTALLKQA
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NEDLERMVLERTSELARARDEAEHFARIDMLTGVANRRYFEEVATMEFARTQRGQQALSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIDIDLFKQVNDRYGHAAGDAVIRAVARLAKESVRESDFVARIGGEEFSVLLPGIGQAP
HEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
AFATAERLRERIAACVVEHDGHSLGFTASFGVSEQVADDPGFSSLLRRADQALYAAKQAG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC
RNQVVVQALEWG
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]