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The map label for this gene is proP [H]

Identifier: 120612981

GI number: 120612981

Start: 4827331

End: 4828989

Strand: Reverse

Name: proP [H]

Synonym: Aave_4345

Alternate gene names: 120612981

Gene position: 4828989-4827331 (Counterclockwise)

Preceding gene: 120612982

Following gene: 120612980

Centisome position: 90.21

GC content: 65.52

Gene sequence:

>1659_bases
ATGGCTACCAATGTCGCTCCCCGGCCGATGTCGCCGGAAGAAAGAAAAGTCATCCTGGCCTCGTCGCTGGGTACCGTTTT
CGAGTGGTACGACTTCTACCTGTACGGTTCGCTCGCCGCCATCATCGCCAAGCAGTTCTTCAGCGGACTGGATGCGGGCT
CCGCCTTCATCTTCGCGCTGCTGGCCTTCGCCGCCGGGTTCATCGTGCGGCCGTTCGGCGCGCTGGTGTTCGGGCGGCTG
GGCGACATGATCGGGCGCAAGTACACCTTCCTGGTCACCATCCTGATCATGGGCCTGTCCACCTTCATCGTGGGGGTCCT
GCCGACCTATGCCAGCATCGGCGTGGCCGCCCCGGTCATCCTGATCGTGCTGCGCATGCTGCAGGGCCTGGCCCTGGGCG
GCGAGTACGGCGGTGCCGCCACCTACGTGGCCGAGCACGCGCCGCAGGGCAAGCGCGGCGCCTACACCTCCTGGATCCAG
ACCACCGCCACGATGGGCCTGTTCCTGTCGCTGCTGGTCATCCTGGGCACCCGCACCGTGATGGGCGAGGAAGCCTTCAC
CGACTGGGGCTGGCGCATCCCGTTCCTCGTCTCCATCCTGCTGCTGGGCATCTCGCTGTGGATCCGGCTGTCGCTGTCGG
AATCGCCCGCCTTCCAGCGGATGAAGGCCGAGGGCAAGACCTCCAAGGCGCCTCTGCGCGAATCGTTCGGCCAATGGAAG
AACCTGAAGATCGTGATCCTGGCGCTGATCGGCCTGACCGCCGGCCAGGCCGTGGTCTGGTACACGGGCCAGTTCTACGC
GCTCTTCTTCCTGACGCAGCAGCTCAAGGTGGATGCCGTCACCGCCAACCTGATGATCGCCGCCGCCCTGCTGATCGGCA
CGCCGTTCTTCGTCGTCTTCGGCGCGCTTTCCGACCGTATCGGCCGCAAGCCCATCATCATGCTGGGCTGCGTGCTGGCC
GTGCTGACGTATTTCCCCGTCTTCAAGGCGCTGACCGAAGCCGCCAACCCCGACCTGGCCGCCGCGCAGGCGAAGAACAA
GGTGGTGATCGTGGCCGACCCGGCCGAGTGTTCGTTCCAGTTCAACCCGACCGGCACGGCCAAGTTCACCAGCTCCTGCG
ACGTCGCCAAGCAGGTGCTGGCCGCCAGCTCGGTGAGCTACGACAACGAGGCCGCGCCGGCCGGCACGCCCGCGGTGATC
AAGATCGGCCAGACCACCATTCCGAGCTACTCCGCCCGCGGCCTGCCCGCCGACGAGGCGAAGGCCAAGGATGCCGCCTT
CAAGAAGGCCGTGGCCGAGACGCTCAAGGCCGACGGCTATCCCTCCAAGGCCGACCCCGCCAAGATGAACAAGGTGATGA
TGATCGTCATCCTGACGTACCTGGTGCTGCTGGTGACCATGGTGTACGGCCCCATCGCGGCGATGCTGGTGGAGATGTTC
CCCACCCGCATCCGCTACACCTCGATGAGCCTGCCCTACCACATCGGCAACGGCTGGTTCGGCGGCCTGCTGCCCACCAT
GTCGTTCGCGATCGTGGCGCAGACCGGCAACATGTACAACGGCCTCTGGTATCCGATCATCATCGCGGGCGTGACGGCCG
TGATCGGCACGCTGTTCATCCGCGAGACCAAGGACGTGGACATCTACGCCAACGACTGA

Upstream 100 bases:

>100_bases
TCGGTCAGATGGTGGTTCGATAGTGCGGCCCAGGTTGCACGCGGCGCCCTGGAGGGGACGCGCGGGCAGTCGCACCGACC
ACCTCATGGAGACAAGCACC

Downstream 100 bases:

>100_bases
CGGGTGACGTCTCCGGGCTGGGGGGCGCGGCACATGCGGTGCCGCGCCCCCGCTTTTTTGGGCAGTGAATGCCGGATGGA
TCGCTTGGGGTGCACAGGTG

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATNVAPRPMSPEERKVILASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFALLAFAAGFIVRPFGALVFGRL
GDMIGRKYTFLVTILIMGLSTFIVGVLPTYASIGVAAPVILIVLRMLQGLALGGEYGGAATYVAEHAPQGKRGAYTSWIQ
TTATMGLFLSLLVILGTRTVMGEEAFTDWGWRIPFLVSILLLGISLWIRLSLSESPAFQRMKAEGKTSKAPLRESFGQWK
NLKIVILALIGLTAGQAVVWYTGQFYALFFLTQQLKVDAVTANLMIAAALLIGTPFFVVFGALSDRIGRKPIIMLGCVLA
VLTYFPVFKALTEAANPDLAAAQAKNKVVIVADPAECSFQFNPTGTAKFTSSCDVAKQVLAASSVSYDNEAAPAGTPAVI
KIGQTTIPSYSARGLPADEAKAKDAAFKKAVAETLKADGYPSKADPAKMNKVMMIVILTYLVLLVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGLLPTMSFAIVAQTGNMYNGLWYPIIIAGVTAVIGTLFIRETKDVDIYAND

Sequences:

>Translated_552_residues
MATNVAPRPMSPEERKVILASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFALLAFAAGFIVRPFGALVFGRL
GDMIGRKYTFLVTILIMGLSTFIVGVLPTYASIGVAAPVILIVLRMLQGLALGGEYGGAATYVAEHAPQGKRGAYTSWIQ
TTATMGLFLSLLVILGTRTVMGEEAFTDWGWRIPFLVSILLLGISLWIRLSLSESPAFQRMKAEGKTSKAPLRESFGQWK
NLKIVILALIGLTAGQAVVWYTGQFYALFFLTQQLKVDAVTANLMIAAALLIGTPFFVVFGALSDRIGRKPIIMLGCVLA
VLTYFPVFKALTEAANPDLAAAQAKNKVVIVADPAECSFQFNPTGTAKFTSSCDVAKQVLAASSVSYDNEAAPAGTPAVI
KIGQTTIPSYSARGLPADEAKAKDAAFKKAVAETLKADGYPSKADPAKMNKVMMIVILTYLVLLVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGLLPTMSFAIVAQTGNMYNGLWYPIIIAGVTAVIGTLFIRETKDVDIYAND
>Mature_551_residues
ATNVAPRPMSPEERKVILASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFALLAFAAGFIVRPFGALVFGRLG
DMIGRKYTFLVTILIMGLSTFIVGVLPTYASIGVAAPVILIVLRMLQGLALGGEYGGAATYVAEHAPQGKRGAYTSWIQT
TATMGLFLSLLVILGTRTVMGEEAFTDWGWRIPFLVSILLLGISLWIRLSLSESPAFQRMKAEGKTSKAPLRESFGQWKN
LKIVILALIGLTAGQAVVWYTGQFYALFFLTQQLKVDAVTANLMIAAALLIGTPFFVVFGALSDRIGRKPIIMLGCVLAV
LTYFPVFKALTEAANPDLAAAQAKNKVVIVADPAECSFQFNPTGTAKFTSSCDVAKQVLAASSVSYDNEAAPAGTPAVIK
IGQTTIPSYSARGLPADEAKAKDAAFKKAVAETLKADGYPSKADPAKMNKVMMIVILTYLVLLVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGLLPTMSFAIVAQTGNMYNGLWYPIIIAGVTAVIGTLFIRETKDVDIYAND

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=326, Percent_Identity=38.9570552147239, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788292, Length=371, Percent_Identity=33.1536388140162, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788942, Length=345, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789941, Length=338, Percent_Identity=36.6863905325444, Blast_Score=159, Evalue=7e-40,
Organism=Escherichia coli, GI87082231, Length=230, Percent_Identity=25.2173913043478, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082404, Length=323, Percent_Identity=25.3869969040248, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59393; Mature: 59261

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNVAPRPMSPEERKVILASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFAL
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGVLPTYASIGVAAPVI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVLRMLQGLALGGEYGGAATYVAEHAPQGKRGAYTSWIQTTATMGLFLSLLVILGTRTV
HHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
MGEEAFTDWGWRIPFLVSILLLGISLWIRLSLSESPAFQRMKAEGKTSKAPLRESFGQWK
CCCHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCHHHHHCCCC
NLKIVILALIGLTAGQAVVWYTGQFYALFFLTQQLKVDAVTANLMIAAALLIGTPFFVVF
CHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
GALSDRIGRKPIIMLGCVLAVLTYFPVFKALTEAANPDLAAAQAKNKVVIVADPAECSFQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCEEE
FNPTGTAKFTSSCDVAKQVLAASSVSYDNEAAPAGTPAVIKIGQTTIPSYSARGLPADEA
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHH
KAKDAAFKKAVAETLKADGYPSKADPAKMNKVMMIVILTYLVLLVTMVYGPIAAMLVEMF
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGLLPTMSFAIVAQTGNMYNGLWYPIIIAGVTAVIGTLFI
HHHHEEEEECCCEEECCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RETKDVDIYAND
CCCCCEEEEECC
>Mature Secondary Structure 
ATNVAPRPMSPEERKVILASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFAL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGVLPTYASIGVAAPVI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVLRMLQGLALGGEYGGAATYVAEHAPQGKRGAYTSWIQTTATMGLFLSLLVILGTRTV
HHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
MGEEAFTDWGWRIPFLVSILLLGISLWIRLSLSESPAFQRMKAEGKTSKAPLRESFGQWK
CCCHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCHHHHHCCCC
NLKIVILALIGLTAGQAVVWYTGQFYALFFLTQQLKVDAVTANLMIAAALLIGTPFFVVF
CHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
GALSDRIGRKPIIMLGCVLAVLTYFPVFKALTEAANPDLAAAQAKNKVVIVADPAECSFQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCEEE
FNPTGTAKFTSSCDVAKQVLAASSVSYDNEAAPAGTPAVIKIGQTTIPSYSARGLPADEA
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHH
KAKDAAFKKAVAETLKADGYPSKADPAKMNKVMMIVILTYLVLLVTMVYGPIAAMLVEMF
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGLLPTMSFAIVAQTGNMYNGLWYPIIIAGVTAVIGTLFI
HHHHEEEEECCCEEECCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
RETKDVDIYAND
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]