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The map label for this gene is purR [H]

Identifier: 120612889

GI number: 120612889

Start: 4713309

End: 4714373

Strand: Direct

Name: purR [H]

Synonym: Aave_4253

Alternate gene names: 120612889

Gene position: 4713309-4714373 (Clockwise)

Preceding gene: 120612885

Following gene: 120612890

Centisome position: 88.05

GC content: 73.8

Gene sequence:

>1065_bases
ATGCAGCGAACGCCGTACCGGCGGCCCGGCCCCGGTACCCGTCCCTTTTCCCACCGCCTTGCCCAGGAGTCCGGATTGGC
CAGCATCAAGGACGTCGCCCAGCGTGCCGGCGTCTCCACCACCACCGTCTCGCGCGTGCTCACCACGCCTGGCGCCGTGC
GTGCCCCGCTGCGCGAGCGCGTGGAGCAGGCCATCGCCGACCTGGGCTACCGGCCCAACCTCGCGGCGCGCAGGCTGCGC
CAGCGCCGCGCATCGATCGTCGGCCTGATCGTCTCGGACATCCGCAGCCCCTTCTTCACCGACGTGGGCCGTGCCGTGGA
GGACGTGGCCTACCGCAACGGCCTTCGGCTCATCCTCTGCAACAGCGACGAGAACCCCGCCAAGGAGCAGTCGTACCTGG
AGCTGATGGCCGACGAGCAGGCCAGCGGCGTGATCCTGTCGCCCACCATGGAGGGGTTGCGGCGCCTGCGGCCCGGGGAC
TGGCCGTTTCCGCTCGTGCTCGTGGACCGCGCGCTCGAAAGCTCCCGCGTGGACCGCGTGGTACTGGACAACCACGCCGC
GGCGCGCCGCGCCACGGAGCACCTGCTGCAATCGGGCTACCGCCGCATCGCCCTGCTCGCCGGCATCCACAGCACCACCG
GCCAGCAGCGCCATGCGGGTTATGCGGACGCCCTGGCGGCGCACGGGCTCGCGCCGCGGCCGCTGTGGCTCGACCCCACG
CGCGACGCGGGGCAGCGCGCCACCGCGCAGTGCCTCGCGGCGCCGCCCGCCGAGCGCCCCGACGCACTGCTCGCCACCAA
CGGCCTGCTGCTGCTCGGCGCCCTGCAGGCCGTGCAGGCCGCGGGCCTGCGCACACCGCAGGACATCGGCATCGCCGGCT
TCGACAACAACGACTGGACCGCCCTGCCCGCGCTGGACGTGACCACCATCGCCCAGCCCACCTACGACATCGGCCGCACC
GCGGCGGAGCTGCTGCTGCAGCGCATGCAGGACCCGGCGCGGCCCGCGCGGCGCGTGGTGCTGGAGGGGGAACTGGTGGC
ACGGGGCTCCAGCGCAAGGTCCTAG

Upstream 100 bases:

>100_bases
ACGAAATCTTCAGAATTTCCCGTCGGGAACGCCCCCGCGGAGGCATGGGGCGGAAAACCGCCGCCACGCCGCTTGCCAGA
TGATTCATGGAATCGTTACC

Downstream 100 bases:

>100_bases
GACTTCGCGCGCCACGGCCCCGCTTCGCACCGCGGGCACGGTGGCACGGGGCCTGCGGCTACCGTCGATGACAGCCACGT
CCGCTCCACGCGGCATGGCC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MQRTPYRRPGPGTRPFSHRLAQESGLASIKDVAQRAGVSTTTVSRVLTTPGAVRAPLRERVEQAIADLGYRPNLAARRLR
QRRASIVGLIVSDIRSPFFTDVGRAVEDVAYRNGLRLILCNSDENPAKEQSYLELMADEQASGVILSPTMEGLRRLRPGD
WPFPLVLVDRALESSRVDRVVLDNHAAARRATEHLLQSGYRRIALLAGIHSTTGQQRHAGYADALAAHGLAPRPLWLDPT
RDAGQRATAQCLAAPPAERPDALLATNGLLLLGALQAVQAAGLRTPQDIGIAGFDNNDWTALPALDVTTIAQPTYDIGRT
AAELLLQRMQDPARPARRVVLEGELVARGSSARS

Sequences:

>Translated_354_residues
MQRTPYRRPGPGTRPFSHRLAQESGLASIKDVAQRAGVSTTTVSRVLTTPGAVRAPLRERVEQAIADLGYRPNLAARRLR
QRRASIVGLIVSDIRSPFFTDVGRAVEDVAYRNGLRLILCNSDENPAKEQSYLELMADEQASGVILSPTMEGLRRLRPGD
WPFPLVLVDRALESSRVDRVVLDNHAAARRATEHLLQSGYRRIALLAGIHSTTGQQRHAGYADALAAHGLAPRPLWLDPT
RDAGQRATAQCLAAPPAERPDALLATNGLLLLGALQAVQAAGLRTPQDIGIAGFDNNDWTALPALDVTTIAQPTYDIGRT
AAELLLQRMQDPARPARRVVLEGELVARGSSARS
>Mature_354_residues
MQRTPYRRPGPGTRPFSHRLAQESGLASIKDVAQRAGVSTTTVSRVLTTPGAVRAPLRERVEQAIADLGYRPNLAARRLR
QRRASIVGLIVSDIRSPFFTDVGRAVEDVAYRNGLRLILCNSDENPAKEQSYLELMADEQASGVILSPTMEGLRRLRPGD
WPFPLVLVDRALESSRVDRVVLDNHAAARRATEHLLQSGYRRIALLAGIHSTTGQQRHAGYADALAAHGLAPRPLWLDPT
RDAGQRATAQCLAAPPAERPDALLATNGLLLLGALQAVQAAGLRTPQDIGIAGFDNNDWTALPALDVTTIAQPTYDIGRT
AAELLLQRMQDPARPARRVVLEGELVARGSSARS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=33.0357142857143, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=32.0588235294118, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790194, Length=337, Percent_Identity=30.2670623145401, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789202, Length=281, Percent_Identity=32.7402135231317, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=30.6188925081433, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789068, Length=277, Percent_Identity=28.158844765343, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=28.1645569620253, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=29.6416938110749, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787580, Length=328, Percent_Identity=29.2682926829268, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=27.8846153846154, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1786268, Length=335, Percent_Identity=25.0746268656716, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=26.3492063492064, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790192, Length=219, Percent_Identity=27.3972602739726, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38402; Mature: 38402

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTPYRRPGPGTRPFSHRLAQESGLASIKDVAQRAGVSTTTVSRVLTTPGAVRAPLRER
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
VEQAIADLGYRPNLAARRLRQRRASIVGLIVSDIRSPFFTDVGRAVEDVAYRNGLRLILC
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEE
NSDENPAKEQSYLELMADEQASGVILSPTMEGLRRLRPGDWPFPLVLVDRALESSRVDRV
CCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEE
VLDNHAAARRATEHLLQSGYRRIALLAGIHSTTGQQRHAGYADALAAHGLAPRPLWLDPT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCEECCCC
RDAGQRATAQCLAAPPAERPDALLATNGLLLLGALQAVQAAGLRTPQDIGIAGFDNNDWT
CCCCHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCHHCCEEECCCCCCE
ALPALDVTTIAQPTYDIGRTAAELLLQRMQDPARPARRVVLEGELVARGSSARS
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHEEECCHHEECCCCCCC
>Mature Secondary Structure
MQRTPYRRPGPGTRPFSHRLAQESGLASIKDVAQRAGVSTTTVSRVLTTPGAVRAPLRER
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
VEQAIADLGYRPNLAARRLRQRRASIVGLIVSDIRSPFFTDVGRAVEDVAYRNGLRLILC
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEE
NSDENPAKEQSYLELMADEQASGVILSPTMEGLRRLRPGDWPFPLVLVDRALESSRVDRV
CCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEE
VLDNHAAARRATEHLLQSGYRRIALLAGIHSTTGQQRHAGYADALAAHGLAPRPLWLDPT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCEECCCC
RDAGQRATAQCLAAPPAERPDALLATNGLLLLGALQAVQAAGLRTPQDIGIAGFDNNDWT
CCCCHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCHHCCEEECCCCCCE
ALPALDVTTIAQPTYDIGRTAAELLLQRMQDPARPARRVVLEGELVARGSSARS
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHEEECCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA