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The map label for this gene is otsA [H]

Identifier: 120612848

GI number: 120612848

Start: 4667709

End: 4669088

Strand: Direct

Name: otsA [H]

Synonym: Aave_4212

Alternate gene names: 120612848

Gene position: 4667709-4669088 (Clockwise)

Preceding gene: 120612847

Following gene: 120612850

Centisome position: 87.2

GC content: 68.84

Gene sequence:

>1380_bases
ATGAGCCGACTCGTCGTCATTTCCAACCGCATCGCCGATCCCCGCAAACCCGCCGCAGGGGGGCTGGCCGTGGCCCTGGG
CGAGACGCTGAGCCGCACCGGCGGCATGTGGTTCGGCTGGAGCGGCACCATCACCGAGGACGGCGTGCCCGGCGAGGGCA
AGCTGCAGACGCGGCAGGCCGGCGGCGTGACGCTGGCCACGGTGGACCTGTGCCAGGAGGACCACGACAGCTACTACGCG
GGCTACAGCAACAGCGTGCTGTGGCCGGTGTTCCACTACCGGCTGGACCTGGCGGATTTCAATGCGGGCTACATCGCGGG
CTACCGGCGCGTGAACCAGATGTTCGCGCGCAAGCTGCTGCCGCTGCTGAAGGAGGACGACATCCTCTGGGTGCACGACT
ACCACCTGATACCGCTGGCGGCAGAGCTGCGCGCGCTCGGCTGCAAGCAGCGCATAGGCTTCTTCCTGCACATTCCCGTG
CCGCCGCCGCTCATCATGGCGGCCATCCCGCAGCACGAATGGCTGATGCGATCGCTGTTCGCCTACGACCTCGTGGGCCT
GCAGAGCGAGGCGGACGTGTCCCACTTCACGCAGTACCTGAGGACCGAAGGCGGCGCCGAAAGCGTGGGCCCGCAGCGGC
TGCGCGCCTTCGGCGGCACGGTGCAGGTGGGGGCCTTCCCGATCGGCATCGACGTGGACGAGTTCTCGCGCCTCACCGCC
GCACCGGACGCCGTGCAGACCTACGAGGCGATGAAGAACGAGTATTCGCGGCGGCGCCTGCTGATGGGCATCGACCGGCT
GGATTACTCCAAGGGCATTCCGCAGCGGGTGCGTGCCTTCCGCGAACTGCTGGCGCGCTATCCCGAGAACCAGCACAGCG
CCACGCTCATCATGATCGCATCCCCGACGCGCGACAGCGTGAACGCCTATGCCGACCTGCGGCACGAACTCGAGGGCCTG
TGCGGAGCCATCAACGGCGACCACGGCGACCTGGACTGGATGCCCGTGCGCTACATCCACCGCACCGTGGCCCGCAAGCG
CGTGCCCGGGCTGTGCCGGGCCTCGGCCGTGGGACTGGTCACGCCGCTGCGCGACGGCATGAACCTCGTGGCCAAGGAAT
ACGTGGCGGCGCAGGATCCGGAAGACCCCGGCGTGCTCGTGCTCTCGCGCTTCGCGGGCGCGGCGGAGCAGATGAAGGAA
GCGCTGCTGGTGAACCCGTACGACACCCAGGGCATGGCCGACTGTATCCAGACCGCGCTGCGCATGCCGCTCGCGGAGCG
CCAGCAGCGCCACCAGGCCCTGATGGACCGCATCCGCCGGCACGACATCCACTGGTGGCGGCGCACCTTCCTGGAGGCGC
TGGAGAAGGCGCAGGACTGA

Upstream 100 bases:

>100_bases
TTCCATGGTGGGCATCATCAATGCCGCCATGCGCCTCTCGGCGCCCTGGGACAGCGTGATCTGACGGGCGCCCGCCTGGA
TTGAAAAACAAGGAATTCCC

Downstream 100 bases:

>100_bases
GCCGGCACGGGCACAATCCGCACATGGCCCAAGGCAAACGCATCTCCGTGGACATCTCCGAGGACGCCCTGCAGGCGATC
CGCGCCCTGGGTGCGGCGGC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSRLVVISNRIADPRKPAAGGLAVALGETLSRTGGMWFGWSGTITEDGVPGEGKLQTRQAGGVTLATVDLCQEDHDSYYA
GYSNSVLWPVFHYRLDLADFNAGYIAGYRRVNQMFARKLLPLLKEDDILWVHDYHLIPLAAELRALGCKQRIGFFLHIPV
PPPLIMAAIPQHEWLMRSLFAYDLVGLQSEADVSHFTQYLRTEGGAESVGPQRLRAFGGTVQVGAFPIGIDVDEFSRLTA
APDAVQTYEAMKNEYSRRRLLMGIDRLDYSKGIPQRVRAFRELLARYPENQHSATLIMIASPTRDSVNAYADLRHELEGL
CGAINGDHGDLDWMPVRYIHRTVARKRVPGLCRASAVGLVTPLRDGMNLVAKEYVAAQDPEDPGVLVLSRFAGAAEQMKE
ALLVNPYDTQGMADCIQTALRMPLAERQQRHQALMDRIRRHDIHWWRRTFLEALEKAQD

Sequences:

>Translated_459_residues
MSRLVVISNRIADPRKPAAGGLAVALGETLSRTGGMWFGWSGTITEDGVPGEGKLQTRQAGGVTLATVDLCQEDHDSYYA
GYSNSVLWPVFHYRLDLADFNAGYIAGYRRVNQMFARKLLPLLKEDDILWVHDYHLIPLAAELRALGCKQRIGFFLHIPV
PPPLIMAAIPQHEWLMRSLFAYDLVGLQSEADVSHFTQYLRTEGGAESVGPQRLRAFGGTVQVGAFPIGIDVDEFSRLTA
APDAVQTYEAMKNEYSRRRLLMGIDRLDYSKGIPQRVRAFRELLARYPENQHSATLIMIASPTRDSVNAYADLRHELEGL
CGAINGDHGDLDWMPVRYIHRTVARKRVPGLCRASAVGLVTPLRDGMNLVAKEYVAAQDPEDPGVLVLSRFAGAAEQMKE
ALLVNPYDTQGMADCIQTALRMPLAERQQRHQALMDRIRRHDIHWWRRTFLEALEKAQD
>Mature_458_residues
SRLVVISNRIADPRKPAAGGLAVALGETLSRTGGMWFGWSGTITEDGVPGEGKLQTRQAGGVTLATVDLCQEDHDSYYAG
YSNSVLWPVFHYRLDLADFNAGYIAGYRRVNQMFARKLLPLLKEDDILWVHDYHLIPLAAELRALGCKQRIGFFLHIPVP
PPLIMAAIPQHEWLMRSLFAYDLVGLQSEADVSHFTQYLRTEGGAESVGPQRLRAFGGTVQVGAFPIGIDVDEFSRLTAA
PDAVQTYEAMKNEYSRRRLLMGIDRLDYSKGIPQRVRAFRELLARYPENQHSATLIMIASPTRDSVNAYADLRHELEGLC
GAINGDHGDLDWMPVRYIHRTVARKRVPGLCRASAVGLVTPLRDGMNLVAKEYVAAQDPEDPGVLVLSRFAGAAEQMKEA
LLVNPYDTQGMADCIQTALRMPLAERQQRHQALMDRIRRHDIHWWRRTFLEALEKAQD

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=458, Percent_Identity=46.5065502183406, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71987012, Length=439, Percent_Identity=27.7904328018223, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI115533560, Length=370, Percent_Identity=29.7297297297297, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI115533558, Length=370, Percent_Identity=29.7297297297297, Blast_Score=161, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=445, Percent_Identity=33.2584269662921, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6320279, Length=427, Percent_Identity=27.6346604215457, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323537, Length=435, Percent_Identity=26.8965517241379, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323917, Length=448, Percent_Identity=24.3303571428571, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19920676, Length=491, Percent_Identity=37.8818737270876, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 51434; Mature: 51302

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVISNRIADPRKPAAGGLAVALGETLSRTGGMWFGWSGTITEDGVPGEGKLQTRQA
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECC
GGVTLATVDLCQEDHDSYYAGYSNSVLWPVFHYRLDLADFNAGYIAGYRRVNQMFARKLL
CCEEEEEHHHHHHCCCCEECCCCCCEEHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHH
PLLKEDDILWVHDYHLIPLAAELRALGCKQRIGFFLHIPVPPPLIMAAIPQHEWLMRSLF
HHHCCCCEEEEECCEEHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCCHHHHHHHHH
AYDLVGLQSEADVSHFTQYLRTEGGAESVGPQRLRAFGGTVQVGAFPIGIDVDEFSRLTA
HHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCEEECCCHHHHHHHHC
APDAVQTYEAMKNEYSRRRLLMGIDRLDYSKGIPQRVRAFRELLARYPENQHSATLIMIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
SPTRDSVNAYADLRHELEGLCGAINGDHGDLDWMPVRYIHRTVARKRVPGLCRASAVGLV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TPLRDGMNLVAKEYVAAQDPEDPGVLVLSRFAGAAEQMKEALLVNPYDTQGMADCIQTAL
HHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
RMPLAERQQRHQALMDRIRRHDIHWWRRTFLEALEKAQD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLVVISNRIADPRKPAAGGLAVALGETLSRTGGMWFGWSGTITEDGVPGEGKLQTRQA
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECC
GGVTLATVDLCQEDHDSYYAGYSNSVLWPVFHYRLDLADFNAGYIAGYRRVNQMFARKLL
CCEEEEEHHHHHHCCCCEECCCCCCEEHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHH
PLLKEDDILWVHDYHLIPLAAELRALGCKQRIGFFLHIPVPPPLIMAAIPQHEWLMRSLF
HHHCCCCEEEEECCEEHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCCHHHHHHHHH
AYDLVGLQSEADVSHFTQYLRTEGGAESVGPQRLRAFGGTVQVGAFPIGIDVDEFSRLTA
HHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCEEECCCHHHHHHHHC
APDAVQTYEAMKNEYSRRRLLMGIDRLDYSKGIPQRVRAFRELLARYPENQHSATLIMIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
SPTRDSVNAYADLRHELEGLCGAINGDHGDLDWMPVRYIHRTVARKRVPGLCRASAVGLV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TPLRDGMNLVAKEYVAAQDPEDPGVLVLSRFAGAAEQMKEALLVNPYDTQGMADCIQTAL
HHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
RMPLAERQQRHQALMDRIRRHDIHWWRRTFLEALEKAQD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA