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The map label for this gene is phaZ1 [H]

Identifier: 120612664

GI number: 120612664

Start: 4475231

End: 4476394

Strand: Reverse

Name: phaZ1 [H]

Synonym: Aave_4027

Alternate gene names: 120612664

Gene position: 4476394-4475231 (Counterclockwise)

Preceding gene: 120612665

Following gene: 120612663

Centisome position: 83.63

GC content: 74.05

Gene sequence:

>1164_bases
ATGGCCGATGCCACGCGTTCCACGCGCGGGGGCCGGCTCGGCGAAGCCACCGAGGCCATCCAGCGCGCGTTGCGCGGGGC
GGCCGCACACGCACACGCACAGGCGGCTGGCGCGTCAGTCGCGGGGCCGCCTCCGGTGTTCCGCGGTGCGCCGGGCCCTG
CGGCGGACCCCAGGGTGCTCGATGGACTGGTCCGCGAGGTGCACGATCCGAACGCGGCGGGTGAGGCCCGCGAGGCGCGC
CGGCCCGCAAGCACGGCACCCCTCCAGGGCTGCGGCGAACAATGGCAATCCGGCAGTTTCAGCCACCAGAACCGCGCGCT
GGCCTACCGCCTCTACCTCCCGCCGGAAGTACCGGGCGACGGTGCACCGCGGCCCATGGTGGTCATGCTGCACGGTTGCA
CCCAGCATGCGGAGGATTTCGCGGCGGGCACGCGCATGAACGCGCTGGCGCGGGAGCTCGGCGTGGTGGTCCTCTACCCG
GAGCAGACACAGCGCGCCAATGCCCAGAAATGCTGGAACTGGTTCAAACCGCAGCACCAGCAGCGCGGGCGCGGTGAGCC
GGCGGTGATCGCCGCCCTCGCGCGTTCGATCGCCACCGCGCACCGGGTGGATCCGGCGCGGGTGTATGTCGCGGGCCTGT
CCGCGGGCGGCGCGATGGCGGATGTGGTCGGGCATTGCTACCCGGACGTGTTCGCTGCCGTGGGCGTGCATTCCGGCCTG
CCATACGGCTGCGCGGCGGATGTCGCCTCGGCGCTGTCGGCGATGCGCAGCGGCCCGGGGCCTGCGCCGGGAGCCACTAC
AGGGGCGCGCAGCACGGCGCTCCCCACCATCGTGTTCCACGGCGATGCGGACACCACCGTCCATGTCCGCAACGCGGAGT
CGCTGGTGGCGGCGCTGCGCAGCGGTTCGGGGCGGGGCGCGGCTTCCGGTGCAGCGGCCGCCCCGCGCGTGACCCGGGGC
CAGTCCGGCCGCGGCGCGCGGCACACGCGCACCGTCTATACGGACGCGGCCGGCCGCGATTCGCTCGAGTACTGGCAACT
GCACGGCGCCGGCCATGCCTGGTCGGGCGGCAGCGCCAGCGGCAGCTATACGGACCCGTCGGGCGTGGATGCGAGCGCGG
AGATGCTGCGCTTTTTCCTCGCGCACCCGCGCGCCGCACGCTGA

Upstream 100 bases:

>100_bases
TCGGCGCACTGCAGGCCAGCAGCGCCGTGAAGACCGCGCTGGCCGGACGCATCCATTGACCCCACGAAAGCACTCCGTGA
ACCCACTCTTCCAGCAACTG

Downstream 100 bases:

>100_bases
ACCCGGCCGGTGCCGCATGCTGCGCCGGCGACCCCCCGGATACTGGCACGGCGCATGGGGGCTTGGGTGCCGGCGGACCG
AATCGTGCCTACACTGGCAC

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MADATRSTRGGRLGEATEAIQRALRGAAAHAHAQAAGASVAGPPPVFRGAPGPAADPRVLDGLVREVHDPNAAGEAREAR
RPASTAPLQGCGEQWQSGSFSHQNRALAYRLYLPPEVPGDGAPRPMVVMLHGCTQHAEDFAAGTRMNALARELGVVVLYP
EQTQRANAQKCWNWFKPQHQQRGRGEPAVIAALARSIATAHRVDPARVYVAGLSAGGAMADVVGHCYPDVFAAVGVHSGL
PYGCAADVASALSAMRSGPGPAPGATTGARSTALPTIVFHGDADTTVHVRNAESLVAALRSGSGRGAASGAAAAPRVTRG
QSGRGARHTRTVYTDAAGRDSLEYWQLHGAGHAWSGGSASGSYTDPSGVDASAEMLRFFLAHPRAAR

Sequences:

>Translated_387_residues
MADATRSTRGGRLGEATEAIQRALRGAAAHAHAQAAGASVAGPPPVFRGAPGPAADPRVLDGLVREVHDPNAAGEAREAR
RPASTAPLQGCGEQWQSGSFSHQNRALAYRLYLPPEVPGDGAPRPMVVMLHGCTQHAEDFAAGTRMNALARELGVVVLYP
EQTQRANAQKCWNWFKPQHQQRGRGEPAVIAALARSIATAHRVDPARVYVAGLSAGGAMADVVGHCYPDVFAAVGVHSGL
PYGCAADVASALSAMRSGPGPAPGATTGARSTALPTIVFHGDADTTVHVRNAESLVAALRSGSGRGAASGAAAAPRVTRG
QSGRGARHTRTVYTDAAGRDSLEYWQLHGAGHAWSGGSASGSYTDPSGVDASAEMLRFFLAHPRAAR
>Mature_386_residues
ADATRSTRGGRLGEATEAIQRALRGAAAHAHAQAAGASVAGPPPVFRGAPGPAADPRVLDGLVREVHDPNAAGEAREARR
PASTAPLQGCGEQWQSGSFSHQNRALAYRLYLPPEVPGDGAPRPMVVMLHGCTQHAEDFAAGTRMNALARELGVVVLYPE
QTQRANAQKCWNWFKPQHQQRGRGEPAVIAALARSIATAHRVDPARVYVAGLSAGGAMADVVGHCYPDVFAAVGVHSGLP
YGCAADVASALSAMRSGPGPAPGATTGARSTALPTIVFHGDADTTVHVRNAESLVAALRSGSGRGAASGAAAAPRVTRGQ
SGRGARHTRTVYTDAAGRDSLEYWQLHGAGHAWSGGSASGSYTDPSGVDASAEMLRFFLAHPRAAR

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39936; Mature: 39805

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADATRSTRGGRLGEATEAIQRALRGAAAHAHAQAAGASVAGPPPVFRGAPGPAADPRVL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
DGLVREVHDPNAAGEAREARRPASTAPLQGCGEQWQSGSFSHQNRALAYRLYLPPEVPGD
HHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
GAPRPMVVMLHGCTQHAEDFAAGTRMNALARELGVVVLYPEQTQRANAQKCWNWFKPQHQ
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHCCHHHHHHHCCCHHH
QRGRGEPAVIAALARSIATAHRVDPARVYVAGLSAGGAMADVVGHCYPDVFAAVGVHSGL
HCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
PYGCAADVASALSAMRSGPGPAPGATTGARSTALPTIVFHGDADTTVHVRNAESLVAALR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHH
SGSGRGAASGAAAAPRVTRGQSGRGARHTRTVYTDAAGRDSLEYWQLHGAGHAWSGGSAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEECCCCCCCCCCCC
GSYTDPSGVDASAEMLRFFLAHPRAAR
CCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADATRSTRGGRLGEATEAIQRALRGAAAHAHAQAAGASVAGPPPVFRGAPGPAADPRVL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
DGLVREVHDPNAAGEAREARRPASTAPLQGCGEQWQSGSFSHQNRALAYRLYLPPEVPGD
HHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
GAPRPMVVMLHGCTQHAEDFAAGTRMNALARELGVVVLYPEQTQRANAQKCWNWFKPQHQ
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHCCHHHHHHHCCCHHH
QRGRGEPAVIAALARSIATAHRVDPARVYVAGLSAGGAMADVVGHCYPDVFAAVGVHSGL
HCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
PYGCAADVASALSAMRSGPGPAPGATTGARSTALPTIVFHGDADTTVHVRNAESLVAALR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHH
SGSGRGAASGAAAAPRVTRGQSGRGARHTRTVYTDAAGRDSLEYWQLHGAGHAWSGGSAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEECCCCCCCCCCCC
GSYTDPSGVDASAEMLRFFLAHPRAAR
CCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]