BacMap

Definition	Acidovorax citrulli AAC00-1 chromosome, complete genome.
Accession	NC_008752
Length	5,352,772

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The map label for this gene is braF [H]

Identifier: 120612459

GI number: 120612459

Start: 4239718

End: 4240467

Strand: Reverse

Name: braF [H]

Synonym: Aave_3818

Alternate gene names: 120612459

Gene position: 4240467-4239718 (Counterclockwise)

Preceding gene: 120612460

Following gene: 120612458

Centisome position: 79.22

GC content: 66.93

Gene sequence:

>750_bases
ATGAACGACCACGTTCCATTGCTCGAGGCGCGCGGGCTCGACGTCGCGTTCGGTGGCATCCATGCGGTGCGGCAGGTGGA
CATGCGCATAGGTCCGGCAGAGCTGATCTGCATCATCGGTCCGAACGGCGCCGGCAAAAGCACGCTGCTGGGGGTTTTGT
CCGGCGCGGTCCGTACCCATGCCGGCAGCCTGGCGATCTCCGGGCGGGACATGACGCGGCAGCTCCCGCACGAATTCGCG
CGCCGGGGAGTGATCCGCAAATTCCAGGGCACGAATGTCTTCCAGTGGCTGAGCGTGCGCGAGAACCTGGTCGTGGCGGC
GCAGGGCGTCGAAGGCGACCGTGCGCGGTCCGCCCCGGATTGCGACGAAATTCTCGCCCTCACCGGGCTGCAGCACCGTG
CCGATGAAACGGCGGAGTGCCTGCCGCACGGCGAACGCCAGTGGCTGGAGATCGGCATGGCACTCATGTGCCGGCCCCGC
CTGCTGTTGCTGGACGAACCCGCCGCAGGCATGAGCGTCGATGGGACCCGCCGCATGGTCGGAATGCTGCGCCAGCTTTC
GCAGGAGATGTCGGTCGTGGTCATCGAGCACGACATCGGCTTCGTGCGCGAACTGGCGTGCCGGACTCTGGTGATGCACC
AGGGCGCCGTGATCCGCCAGGGCGCCTTCGCGGAAATCGAACGGGACGAACACATACGGCACATCTATCTGGGCCGGAGC
AAGGAGGCGCGCCATGCTGGACGTGCGTGA

Upstream 100 bases:

>100_bases
TGCGCAGGCGCGGCCCCGGGCATGCTTCCAGCGCCGCGCCCGCAAAGCAGTCCCGGCCGGGCCGCCCGGCAGGCGTGTCC
ACGAATGCGGGGAGCCGGAC

Downstream 100 bases:

>100_bases
TCTCACGGCGGGCTACGGTGGCCCTGCCGTGATCGACGGGGTGCAGTTCCACCTCGCCAAGGGAGAAGTCCTGGGAATCC
TCGGGCGCAACGGCGCCGGC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNDHVPLLEARGLDVAFGGIHAVRQVDMRIGPAELICIIGPNGAGKSTLLGVLSGAVRTHAGSLAISGRDMTRQLPHEFA
RRGVIRKFQGTNVFQWLSVRENLVVAAQGVEGDRARSAPDCDEILALTGLQHRADETAECLPHGERQWLEIGMALMCRPR
LLLLDEPAAGMSVDGTRRMVGMLRQLSQEMSVVVIEHDIGFVRELACRTLVMHQGAVIRQGAFAEIERDEHIRHIYLGRS
KEARHAGRA

Sequences:

>Translated_249_residues
MNDHVPLLEARGLDVAFGGIHAVRQVDMRIGPAELICIIGPNGAGKSTLLGVLSGAVRTHAGSLAISGRDMTRQLPHEFA
RRGVIRKFQGTNVFQWLSVRENLVVAAQGVEGDRARSAPDCDEILALTGLQHRADETAECLPHGERQWLEIGMALMCRPR
LLLLDEPAAGMSVDGTRRMVGMLRQLSQEMSVVVIEHDIGFVRELACRTLVMHQGAVIRQGAFAEIERDEHIRHIYLGRS
KEARHAGRA
>Mature_249_residues
MNDHVPLLEARGLDVAFGGIHAVRQVDMRIGPAELICIIGPNGAGKSTLLGVLSGAVRTHAGSLAISGRDMTRQLPHEFA
RRGVIRKFQGTNVFQWLSVRENLVVAAQGVEGDRARSAPDCDEILALTGLQHRADETAECLPHGERQWLEIGMALMCRPR
LLLLDEPAAGMSVDGTRRMVGMLRQLSQEMSVVVIEHDIGFVRELACRTLVMHQGAVIRQGAFAEIERDEHIRHIYLGRS
KEARHAGRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI116734710, Length=194, Percent_Identity=27.319587628866, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI153792144, Length=169, Percent_Identity=31.3609467455621, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI105990541, Length=203, Percent_Identity=24.6305418719212, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI27436953, Length=215, Percent_Identity=26.9767441860465, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27262624, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI27262626, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI46592956, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592915, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592978, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27881501, Length=208, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI30795238, Length=208, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592971, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592964, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592898, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27477115, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=258, Percent_Identity=30.6201550387597, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1789593, Length=235, Percent_Identity=32.3404255319149, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082268, Length=223, Percent_Identity=32.7354260089686, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788225, Length=237, Percent_Identity=29.535864978903, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787792, Length=232, Percent_Identity=29.7413793103448, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790525, Length=251, Percent_Identity=27.8884462151394, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87081791, Length=234, Percent_Identity=29.9145299145299, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787029, Length=218, Percent_Identity=27.5229357798165, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787089, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790190, Length=244, Percent_Identity=27.0491803278689, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786319, Length=220, Percent_Identity=28.1818181818182, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787370, Length=223, Percent_Identity=27.8026905829596, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081709, Length=231, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789672, Length=227, Percent_Identity=25.5506607929515, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788761, Length=234, Percent_Identity=26.4957264957265, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789991, Length=215, Percent_Identity=27.4418604651163, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786563, Length=211, Percent_Identity=28.436018957346, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790544, Length=253, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994997, Length=244, Percent_Identity=28.2786885245902, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786872, Length=187, Percent_Identity=28.8770053475936, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994943, Length=225, Percent_Identity=32.4444444444444, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1786398, Length=211, Percent_Identity=25.5924170616114, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788450, Length=218, Percent_Identity=29.8165137614679, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787712, Length=226, Percent_Identity=27.8761061946903, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786803, Length=246, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790467, Length=213, Percent_Identity=26.7605633802817, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789963, Length=237, Percent_Identity=27.8481012658228, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI48995001, Length=252, Percent_Identity=23.8095238095238, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789873, Length=206, Percent_Identity=29.126213592233, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788210, Length=235, Percent_Identity=25.531914893617, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790162, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788472, Length=246, Percent_Identity=23.1707317073171, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI145693107, Length=245, Percent_Identity=29.3877551020408, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081834, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788897, Length=247, Percent_Identity=25.1012145748988, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI48994883, Length=237, Percent_Identity=27.4261603375527, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786253, Length=215, Percent_Identity=26.9767441860465, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786345, Length=205, Percent_Identity=30.2439024390244, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788165, Length=201, Percent_Identity=28.8557213930348, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082267, Length=214, Percent_Identity=27.5700934579439, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787758, Length=208, Percent_Identity=25.9615384615385, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787164, Length=219, Percent_Identity=26.9406392694064, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI87081782, Length=227, Percent_Identity=29.5154185022026, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788506, Length=250, Percent_Identity=29.2, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788644, Length=196, Percent_Identity=28.0612244897959, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787105, Length=225, Percent_Identity=24.8888888888889, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789962, Length=250, Percent_Identity=24, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786981, Length=201, Percent_Identity=30.8457711442786, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787112, Length=212, Percent_Identity=28.7735849056604, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17510237, Length=216, Percent_Identity=25.462962962963, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558664, Length=210, Percent_Identity=24.2857142857143, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=198, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=198, Percent_Identity=25.2525252525253, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565938, Length=214, Percent_Identity=25.2336448598131, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193202349, Length=218, Percent_Identity=23.8532110091743, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539902, Length=224, Percent_Identity=28.125, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193211017, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193211015, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71996809, Length=221, Percent_Identity=25.7918552036199, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=215, Percent_Identity=25.1162790697674, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=213, Percent_Identity=25.3521126760563, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6324985, Length=227, Percent_Identity=24.6696035242291, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221512771, Length=207, Percent_Identity=23.1884057971014, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574533, Length=198, Percent_Identity=30.3030303030303, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28573571, Length=218, Percent_Identity=25.2293577981651, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19920532, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580930, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574150, Length=209, Percent_Identity=25.3588516746411, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI116007328, Length=209, Percent_Identity=25.3588516746411, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28573573, Length=200, Percent_Identity=25.5, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85724858, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI85724856, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24580547, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24580551, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24580549, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24580545, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24666092, Length=215, Percent_Identity=22.7906976744186, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24643674, Length=215, Percent_Identity=25.1162790697674, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24659289, Length=217, Percent_Identity=25.8064516129032, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653245, Length=222, Percent_Identity=24.7747747747748, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27356; Mature: 27356

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDHVPLLEARGLDVAFGGIHAVRQVDMRIGPAELICIIGPNGAGKSTLLGVLSGAVRTH
CCCCCCCEECCCCEEEHHHHHHHHHHHCEECCEEEEEEECCCCCCHHHHHHHHHHHHHHC
AGSLAISGRDMTRQLPHEFARRGVIRKFQGTNVFQWLSVRENLVVAAQGVEGDRARSAPD
CCCEEECCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCC
CDEILALTGLQHRADETAECLPHGERQWLEIGMALMCRPRLLLLDEPAAGMSVDGTRRMV
HHHHHHHHCCHHHCCHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
GMLRQLSQEMSVVVIEHDIGFVRELACRTLVMHQGAVIRQGAFAEIERDEHIRHIYLGRS
HHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCHHCCCCHHHCCCCCCEEEEEECCC
KEARHAGRA
CHHHCCCCC
>Mature Secondary Structure
MNDHVPLLEARGLDVAFGGIHAVRQVDMRIGPAELICIIGPNGAGKSTLLGVLSGAVRTH
CCCCCCCEECCCCEEEHHHHHHHHHHHCEECCEEEEEEECCCCCCHHHHHHHHHHHHHHC
AGSLAISGRDMTRQLPHEFARRGVIRKFQGTNVFQWLSVRENLVVAAQGVEGDRARSAPD
CCCEEECCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCC
CDEILALTGLQHRADETAECLPHGERQWLEIGMALMCRPRLLLLDEPAAGMSVDGTRRMV
HHHHHHHHCCHHHCCHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
GMLRQLSQEMSVVVIEHDIGFVRELACRTLVMHQGAVIRQGAFAEIERDEHIRHIYLGRS
HHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCHHCCCCHHHCCCCCCEEEEEECCC
KEARHAGRA
CHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]