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The map label for this gene is lcfB [H]

Identifier: 120612445

GI number: 120612445

Start: 4225375

End: 4226964

Strand: Direct

Name: lcfB [H]

Synonym: Aave_3804

Alternate gene names: 120612445

Gene position: 4225375-4226964 (Clockwise)

Preceding gene: 120612444

Following gene: 120612446

Centisome position: 78.94

GC content: 68.99

Gene sequence:

>1590_bases
ATGCGCAATCTCGTTGAACTGATATGCCACAGCGCCGACCGCGCTCCCGGCGCACAAGCCCTGGTCGATGGAGACCATCG
GCTGGACTACGCCACGCTGCGCGACCATGTGCTGTCGATCGCCGCGGGCCTGGCCGCCGGTGGCCTGCAGCGCGGAGACC
GCGTCGCCGTCTTCCTGGACAAGCGCGTGGAAACCGTCGAGGCCTTCTTCGCCATCGCAGCGGCGGGCGGCGTTTTCGTG
CCGGTCAACCCGCTGCTGAAACCCGAGCAGGTGGTCCACATTTTGCAAGACAGCGGCGCCAGGAGCCTGGTGACCTCGGC
AACCCGCCTGGGTTTGCTGGCGGCGCAGGCCCCCCGCCTTCCGGCGCTGGCGGAAACCTTCCTGATCGATGCTCCCGACG
CCCGCCTCCCGGTTCTGGGGTTCGCCGAGACGCGGGTCTGGCAGGCGCTGCTGGACTTCGCTCCGCTGGACCGCGGCCAG
ATCGACACGATCGACAGCGACCTGGCCGCGATCCTCTACACATCCGGCTCCACGGGTTTGCCCAAGGGCGTGATGCTGAC
GCACCACAATCTGCTCGAAGGCGCCTGGAGCGTGGCGACCTACCTCGGCAACCACGGCGAGGACCGCATCCTGTGCGCGC
TGCCGCTCAGCTTCGATGCGGGTTTCAGCCAGCTCACCACTGCGTTCCACGCGGGTGCGGCGGCGGTGCTCGTCAACTAC
CTGGCGCCGGGCGACGTGATCGCGGCGTGCGAGCGCGAGCGCATCACAGGCATCACCGCGGTTCCCCCGCTCTTGATCCA
GCTCTGCAGCGCTTCCTGGCCGCCCGCGGCCGCAGCGCAGCTGCGCTACTTCGCGAATACCGGAGGCCGCATGCCGAAAC
CCGTGCTGGCCAAGCTCCGCGAGCTGTTCCCGGCCGCCCGGCCGTTTCTGATGTACGGCCTGACCGAGGCATTCCGTTCC
ACCTACCTGCCTCCGGAAGAAGCCGACCGGCGCCCCGACTCCATCGGCAAGGCCGTGCCGAACGCCCGCATTCTCGTGGT
ACGCCCGGACGGCACTCCCTGCGATGCGGACGAAACCGGCGAACTGGTGCATGTCGGGGCCTTCGTGGCGCGGGGCTACT
GGCGCGATCCGGAGCGCACCGCGCAACGCTTCCGGCCTTCCCCCGAAGCCCGGCCGTGGCCCGGCATGGTGCCGGAGCAG
GCCGTGTGGTCCGGCGATCTCGTTCGACGGGACTCCGAGGGTTTTTTGTACTTCGTGGGCCGCAACGACGGCCTGATCAA
GACCTCCGGCTACCGGGTGAGCCCCGAGGAAATCGAGGAAACGGTGCTGGCGAGCGGACTGGCAGGCGAGGTGGTCGCGG
TGGGCATACCGGACGATGCGCTCGGGCAGGCGATCGTCCTCGTGGTGGCGCCGCCTCCCCGCGGCAGCCTCGACGCCGAA
GCCATTCTCACGCACTGCCGGCAGCGGCTGCCGTCCTACATGGTGCCCCGCGCCATCCACGTGCGCAGCACGATCCAGCG
CAATCCCAACGGCAAGTTCGACCGCGCGGCACTGCAGCGCGAACTCTCCATCGCAACGGAGGCGGTATGA

Upstream 100 bases:

>100_bases
ATGGCGCTCGGCTTCGGCGTGGCGCTCGTGCTGGTGCTGCATCGCGTACGACGGCGGCTCACCCGCCTGAACGACGTTTT
TCAACCGACGGAGGAAATCC

Downstream 100 bases:

>100_bases
CGATCCTCGACACCTTCACGACCCGGGACGGCGCTATCGAAATCGGCGGCCTGCCCCTGTCACGCCTGGCGGAGCGGGCC
GGCCGCACGCCGTTCTTCGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MRNLVELICHSADRAPGAQALVDGDHRLDYATLRDHVLSIAAGLAAGGLQRGDRVAVFLDKRVETVEAFFAIAAAGGVFV
PVNPLLKPEQVVHILQDSGARSLVTSATRLGLLAAQAPRLPALAETFLIDAPDARLPVLGFAETRVWQALLDFAPLDRGQ
IDTIDSDLAAILYTSGSTGLPKGVMLTHHNLLEGAWSVATYLGNHGEDRILCALPLSFDAGFSQLTTAFHAGAAAVLVNY
LAPGDVIAACERERITGITAVPPLLIQLCSASWPPAAAAQLRYFANTGGRMPKPVLAKLRELFPAARPFLMYGLTEAFRS
TYLPPEEADRRPDSIGKAVPNARILVVRPDGTPCDADETGELVHVGAFVARGYWRDPERTAQRFRPSPEARPWPGMVPEQ
AVWSGDLVRRDSEGFLYFVGRNDGLIKTSGYRVSPEEIEETVLASGLAGEVVAVGIPDDALGQAIVLVVAPPPRGSLDAE
AILTHCRQRLPSYMVPRAIHVRSTIQRNPNGKFDRAALQRELSIATEAV

Sequences:

>Translated_529_residues
MRNLVELICHSADRAPGAQALVDGDHRLDYATLRDHVLSIAAGLAAGGLQRGDRVAVFLDKRVETVEAFFAIAAAGGVFV
PVNPLLKPEQVVHILQDSGARSLVTSATRLGLLAAQAPRLPALAETFLIDAPDARLPVLGFAETRVWQALLDFAPLDRGQ
IDTIDSDLAAILYTSGSTGLPKGVMLTHHNLLEGAWSVATYLGNHGEDRILCALPLSFDAGFSQLTTAFHAGAAAVLVNY
LAPGDVIAACERERITGITAVPPLLIQLCSASWPPAAAAQLRYFANTGGRMPKPVLAKLRELFPAARPFLMYGLTEAFRS
TYLPPEEADRRPDSIGKAVPNARILVVRPDGTPCDADETGELVHVGAFVARGYWRDPERTAQRFRPSPEARPWPGMVPEQ
AVWSGDLVRRDSEGFLYFVGRNDGLIKTSGYRVSPEEIEETVLASGLAGEVVAVGIPDDALGQAIVLVVAPPPRGSLDAE
AILTHCRQRLPSYMVPRAIHVRSTIQRNPNGKFDRAALQRELSIATEAV
>Mature_529_residues
MRNLVELICHSADRAPGAQALVDGDHRLDYATLRDHVLSIAAGLAAGGLQRGDRVAVFLDKRVETVEAFFAIAAAGGVFV
PVNPLLKPEQVVHILQDSGARSLVTSATRLGLLAAQAPRLPALAETFLIDAPDARLPVLGFAETRVWQALLDFAPLDRGQ
IDTIDSDLAAILYTSGSTGLPKGVMLTHHNLLEGAWSVATYLGNHGEDRILCALPLSFDAGFSQLTTAFHAGAAAVLVNY
LAPGDVIAACERERITGITAVPPLLIQLCSASWPPAAAAQLRYFANTGGRMPKPVLAKLRELFPAARPFLMYGLTEAFRS
TYLPPEEADRRPDSIGKAVPNARILVVRPDGTPCDADETGELVHVGAFVARGYWRDPERTAQRFRPSPEARPWPGMVPEQ
AVWSGDLVRRDSEGFLYFVGRNDGLIKTSGYRVSPEEIEETVLASGLAGEVVAVGIPDDALGQAIVLVVAPPPRGSLDAE
AILTHCRQRLPSYMVPRAIHVRSTIQRNPNGKFDRAALQRELSIATEAV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=24.4565217391304, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI187761345, Length=547, Percent_Identity=26.691042047532, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI187761343, Length=547, Percent_Identity=26.691042047532, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=26.1386138613861, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI115511026, Length=504, Percent_Identity=24.8015873015873, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28416953, Length=531, Percent_Identity=23.728813559322, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=557, Percent_Identity=29.2639138240575, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI221142682, Length=508, Percent_Identity=28.3464566929134, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693145, Length=525, Percent_Identity=27.4285714285714, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786801, Length=538, Percent_Identity=28.0669144981413, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790505, Length=529, Percent_Identity=24.3856332703214, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786810, Length=521, Percent_Identity=22.0729366602687, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=558, Percent_Identity=25.089605734767, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=511, Percent_Identity=23.679060665362, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=523, Percent_Identity=22.9445506692161, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=523, Percent_Identity=22.9445506692161, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=523, Percent_Identity=22.9445506692161, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=551, Percent_Identity=24.5009074410163, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=500, Percent_Identity=23.4, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=470, Percent_Identity=24.6808510638298, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=515, Percent_Identity=23.495145631068, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=526, Percent_Identity=22.4334600760456, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=573, Percent_Identity=21.4659685863874, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=493, Percent_Identity=22.7180527383367, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24581924, Length=523, Percent_Identity=23.3269598470363, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=24.1444866920152, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356947, Length=532, Percent_Identity=22.1804511278195, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=23.8955823293173, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648260, Length=501, Percent_Identity=24.5508982035928, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648676, Length=498, Percent_Identity=26.9076305220884, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552801, Length=549, Percent_Identity=24.0437158469945, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550496, Length=549, Percent_Identity=24.0437158469945, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364798, Length=533, Percent_Identity=22.8893058161351, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=374, Percent_Identity=24.8663101604278, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583465, Length=533, Percent_Identity=22.8893058161351, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583463, Length=533, Percent_Identity=22.8893058161351, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922652, Length=494, Percent_Identity=22.8744939271255, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653035, Length=351, Percent_Identity=25.3561253561254, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281365686, Length=377, Percent_Identity=22.2811671087533, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161076582, Length=358, Percent_Identity=25.9776536312849, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358303, Length=365, Percent_Identity=23.2876712328767, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648253, Length=369, Percent_Identity=22.7642276422764, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648255, Length=369, Percent_Identity=22.7642276422764, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=22.972972972973, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=364, Percent_Identity=23.6263736263736, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56872; Mature: 56872

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLVELICHSADRAPGAQALVDGDHRLDYATLRDHVLSIAAGLAAGGLQRGDRVAVFLD
CCHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
KRVETVEAFFAIAAAGGVFVPVNPLLKPEQVVHILQDSGARSLVTSATRLGLLAAQAPRL
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
PALAETFLIDAPDARLPVLGFAETRVWQALLDFAPLDRGQIDTIDSDLAAILYTSGSTGL
CHHHHHHEEECCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCC
PKGVMLTHHNLLEGAWSVATYLGNHGEDRILCALPLSFDAGFSQLTTAFHAGAAAVLVNY
CCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHH
LAPGDVIAACERERITGITAVPPLLIQLCSASWPPAAAAQLRYFANTGGRMPKPVLAKLR
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
ELFPAARPFLMYGLTEAFRSTYLPPEEADRRPDSIGKAVPNARILVVRPDGTPCDADETG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCC
ELVHVGAFVARGYWRDPERTAQRFRPSPEARPWPGMVPEQAVWSGDLVRRDSEGFLYFVG
CEEEEHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEECCCCEEEEEE
RNDGLIKTSGYRVSPEEIEETVLASGLAGEVVAVGIPDDALGQAIVLVVAPPPRGSLDAE
CCCCEEEECCEECCHHHHHHHHHHCCCCCCEEEEECCCHHCCCEEEEEEECCCCCCCCHH
AILTHCRQRLPSYMVPRAIHVRSTIQRNPNGKFDRAALQRELSIATEAV
HHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNLVELICHSADRAPGAQALVDGDHRLDYATLRDHVLSIAAGLAAGGLQRGDRVAVFLD
CCHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
KRVETVEAFFAIAAAGGVFVPVNPLLKPEQVVHILQDSGARSLVTSATRLGLLAAQAPRL
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
PALAETFLIDAPDARLPVLGFAETRVWQALLDFAPLDRGQIDTIDSDLAAILYTSGSTGL
CHHHHHHEEECCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCC
PKGVMLTHHNLLEGAWSVATYLGNHGEDRILCALPLSFDAGFSQLTTAFHAGAAAVLVNY
CCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHH
LAPGDVIAACERERITGITAVPPLLIQLCSASWPPAAAAQLRYFANTGGRMPKPVLAKLR
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
ELFPAARPFLMYGLTEAFRSTYLPPEEADRRPDSIGKAVPNARILVVRPDGTPCDADETG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCC
ELVHVGAFVARGYWRDPERTAQRFRPSPEARPWPGMVPEQAVWSGDLVRRDSEGFLYFVG
CEEEEHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEECCCCEEEEEE
RNDGLIKTSGYRVSPEEIEETVLASGLAGEVVAVGIPDDALGQAIVLVVAPPPRGSLDAE
CCCCEEEECCEECCHHHHHHHHHHCCCCCCEEEEECCCHHCCCEEEEEEECCCCCCCCHH
AILTHCRQRLPSYMVPRAIHVRSTIQRNPNGKFDRAALQRELSIATEAV
HHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]