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The map label for this gene is mscL

Identifier: 120612341

GI number: 120612341

Start: 4074172

End: 4074603

Strand: Direct

Name: mscL

Synonym: Aave_3697

Alternate gene names: 120612341

Gene position: 4074172-4074603 (Clockwise)

Preceding gene: 120612335

Following gene: 120612345

Centisome position: 76.11

GC content: 63.66

Gene sequence:

>432_bases
ATGGGTATTGCGAAGGAGTTCCGGGAGTTCGCGGTGAAGGGAAATGTGATAGATCTCGCCGTAGGCGTGATCATCGGCGG
CGCGTTCGGGAAGATCGTGGACTCGCTCGTGAACGACGTCATCATGCCCATCGTCGGGCTGGTATTCGGCAGGCTGGATT
TCTCGAATCTCTTTCTCGTGCTCGGCAGTGTGCCGCCCGGCACCCCGGCCACGCTGGACGCCCTGCGCAAGGCGGGCGTA
CCCGTGCTCGCCCACGGCAGTTTCATTACCGTCGCGGTGAACTTCCTGATTCTCGCGTTCATCATTTTCATGATGGTCAA
GCAAATCAACCGGCTCAAGCGCGCGGCGCCGCCCGCACCGCCCGCCACCCCGGCCGCGCCGCCGGAGGACATCGTGCTGC
TGCGCGAGATCCGCGACAGTCTCAGGCGCTGA

Upstream 100 bases:

>100_bases
CACAAATTGTAGCGGCGCAAGTAAGGTTAATTCCAGGGGTTATCACCCCCGCGGCTCGGCAATACTGCAAGGGTTTTCAG
CTATATCAAGGAGAGGCGAA

Downstream 100 bases:

>100_bases
CGGCGCCGGGAACCGGACCCGCCAGGCGGCGCGCCGCCAGCACGGCCTCGACGAGGCTCGATGCGTCGGCCACGCCGCGG
CCGGCGATGTCGAAGGCCGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MGIAKEFREFAVKGNVIDLAVGVIIGGAFGKIVDSLVNDVIMPIVGLVFGRLDFSNLFLVLGSVPPGTPATLDALRKAGV
PVLAHGSFITVAVNFLILAFIIFMMVKQINRLKRAAPPAPPATPAAPPEDIVLLREIRDSLRR

Sequences:

>Translated_143_residues
MGIAKEFREFAVKGNVIDLAVGVIIGGAFGKIVDSLVNDVIMPIVGLVFGRLDFSNLFLVLGSVPPGTPATLDALRKAGV
PVLAHGSFITVAVNFLILAFIIFMMVKQINRLKRAAPPAPPATPAAPPEDIVLLREIRDSLRR
>Mature_142_residues
GIAKEFREFAVKGNVIDLAVGVIIGGAFGKIVDSLVNDVIMPIVGLVFGRLDFSNLFLVLGSVPPGTPATLDALRKAGVP
VLAHGSFITVAVNFLILAFIIFMMVKQINRLKRAAPPAPPATPAAPPEDIVLLREIRDSLRR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=143, Percent_Identity=49.6503496503497, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_ACIAC (A1TTF7)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_972019.1
- ProteinModelPortal:   A1TTF7
- SMR:   A1TTF7
- STRING:   A1TTF7
- GeneID:   4666374
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3697
- NMPDR:   fig|397945.5.peg.3158
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   A1TTF7
- ProtClustDB:   PRK13952
- BioCyc:   AAVE397945:AAVE_3697-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15255; Mature: 15123

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17012dec)-; HASH(0x17333de0)-; HASH(0x1652df54)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIAKEFREFAVKGNVIDLAVGVIIGGAFGKIVDSLVNDVIMPIVGLVFGRLDFSNLFLV
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LGSVPPGTPATLDALRKAGVPVLAHGSFITVAVNFLILAFIIFMMVKQINRLKRAAPPAP
CCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PATPAAPPEDIVLLREIRDSLRR
CCCCCCCHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
GIAKEFREFAVKGNVIDLAVGVIIGGAFGKIVDSLVNDVIMPIVGLVFGRLDFSNLFLV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LGSVPPGTPATLDALRKAGVPVLAHGSFITVAVNFLILAFIIFMMVKQINRLKRAAPPAP
CCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PATPAAPPEDIVLLREIRDSLRR
CCCCCCCHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA