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The map label for this gene is ispB [H]

Identifier: 120612320

GI number: 120612320

Start: 4052389

End: 4053423

Strand: Direct

Name: ispB [H]

Synonym: Aave_3676

Alternate gene names: 120612320

Gene position: 4052389-4053423 (Clockwise)

Preceding gene: 120612315

Following gene: 120612323

Centisome position: 75.71

GC content: 65.7

Gene sequence:

>1035_bases
GTGCTGCATGGCCGTTCCTATAATGAGCCCTTCACCTCGCAGACCGCCCTCGCCTTGCCCGCCCAGCCCCATACCTCGGC
CATCCTTGCACTGATTGCAGACGACATGCATGAGGTGGACAAGGTCATCGGCCTCCGACTGCAGTCTTCCGTCCCCCTCA
TCGGCGAGATTGCCCGCTACATCATCTCCGCCGGCGGAAAGCGGCTGCGCCCGGCGCTGCTGCTCCTGATGTCCGGTGCG
CTGGGCTACCAGGAGGCCCAGCGGTTCAACCTGGCGGCGGTGATCGAATTCATCCACACCGCCACGCTGCTGCACGACGA
CGTGGTGGACGAATCCACGATGCGGCGCGGACGCCCCACCGCGAACGAGAATTTCGGCAACCCCGCGAGCGTGCTGGTCG
GCGACTTCCTGCACTCGCGGGCCTTCCAGATGATGGTGGACGCGGACGACATGCGCGTCATGCAGATCCTGTCGGAAGCG
ACCAACATCATCGCGGAGGGCGAAGTCCAGCAGCTCATGAACACCCATGACGCGACGCTCGACGAACAGGGCTACCTGGA
AGTCATCCGCTCCAAGACCGCCAAGCTGTTCGAGGCCAGCGCCCGCCTCGCGGCCGTGCTGGCCCGCGCCCCCGCAGCCA
TCGAAGAGGCCTGCGCCGAATACGGCAAGGCACTGGGCACGGCCTTCCAGGTGATCGACGACGTGCTGGACTACGACGGC
AACAGCGCCGAAATGGGCAAGAACCTCGGCGACGACCTGCGCGAGGGCAAGGTCACCCTGCCCCTGATCATCGCGATGCA
GCGCGGCGACGCGGCCCAGCGGCAGCTGATCGAGACGGCCATCCAGCAGGGATCGCTCGACGAGCTGCCCGCCATCCTGG
CGATCGTGAAGGCCACCGGCGCCCTGGAGGCCACGCGCGCGGCCGCCGCCGCCCAGGCGCAGGAAGCCATTCGCGCACTA
TCCGCACTTCCTTCCAATAAGTACACCGAAGGTTTGCTACAATTGGCGTCTCAGCTATTAGACAGGCGCGTTTGA

Upstream 100 bases:

>100_bases
AAAACGTTTTTACGGAACCACGCACTATAGCACGAGACACCCGTTGCTGTATAGAGGGGCGACAGGCAGCCGCACCGCAG
GCGCCGGCCGCTTGCTCCGG

Downstream 100 bases:

>100_bases
ACAGGCGCAGCAGGTCTTCCAACACGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTT
CGAATCCTCTCACCCCGACC

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLHGRSYNEPFTSQTALALPAQPHTSAILALIADDMHEVDKVIGLRLQSSVPLIGEIARYIISAGGKRLRPALLLLMSGA
LGYQEAQRFNLAAVIEFIHTATLLHDDVVDESTMRRGRPTANENFGNPASVLVGDFLHSRAFQMMVDADDMRVMQILSEA
TNIIAEGEVQQLMNTHDATLDEQGYLEVIRSKTAKLFEASARLAAVLARAPAAIEEACAEYGKALGTAFQVIDDVLDYDG
NSAEMGKNLGDDLREGKVTLPLIIAMQRGDAAQRQLIETAIQQGSLDELPAILAIVKATGALEATRAAAAAQAQEAIRAL
SALPSNKYTEGLLQLASQLLDRRV

Sequences:

>Translated_344_residues
MLHGRSYNEPFTSQTALALPAQPHTSAILALIADDMHEVDKVIGLRLQSSVPLIGEIARYIISAGGKRLRPALLLLMSGA
LGYQEAQRFNLAAVIEFIHTATLLHDDVVDESTMRRGRPTANENFGNPASVLVGDFLHSRAFQMMVDADDMRVMQILSEA
TNIIAEGEVQQLMNTHDATLDEQGYLEVIRSKTAKLFEASARLAAVLARAPAAIEEACAEYGKALGTAFQVIDDVLDYDG
NSAEMGKNLGDDLREGKVTLPLIIAMQRGDAAQRQLIETAIQQGSLDELPAILAIVKATGALEATRAAAAAQAQEAIRAL
SALPSNKYTEGLLQLASQLLDRRV
>Mature_344_residues
MLHGRSYNEPFTSQTALALPAQPHTSAILALIADDMHEVDKVIGLRLQSSVPLIGEIARYIISAGGKRLRPALLLLMSGA
LGYQEAQRFNLAAVIEFIHTATLLHDDVVDESTMRRGRPTANENFGNPASVLVGDFLHSRAFQMMVDADDMRVMQILSEA
TNIIAEGEVQQLMNTHDATLDEQGYLEVIRSKTAKLFEASARLAAVLARAPAAIEEACAEYGKALGTAFQVIDDVLDYDG
NSAEMGKNLGDDLREGKVTLPLIIAMQRGDAAQRQLIETAIQQGSLDELPAILAIVKATGALEATRAAAAAQAQEAIRAL
SALPSNKYTEGLLQLASQLLDRRV

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=296, Percent_Identity=31.4189189189189, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4758430, Length=273, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI83700220, Length=273, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1789578, Length=307, Percent_Identity=51.7915309446254, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1786623, Length=259, Percent_Identity=33.5907335907336, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17505681, Length=321, Percent_Identity=33.6448598130841, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=30.4635761589404, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=237, Percent_Identity=24.4725738396624, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24651612, Length=309, Percent_Identity=30.0970873786408, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281365769, Length=279, Percent_Identity=24.3727598566308, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24660002, Length=279, Percent_Identity=24.3727598566308, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37069; Mature: 37069

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGRSYNEPFTSQTALALPAQPHTSAILALIADDMHEVDKVIGLRLQSSVPLIGEIARY
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IISAGGKRLRPALLLLMSGALGYQEAQRFNLAAVIEFIHTATLLHDDVVDESTMRRGRPT
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCC
ANENFGNPASVLVGDFLHSRAFQMMVDADDMRVMQILSEATNIIAEGEVQQLMNTHDATL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
DEQGYLEVIRSKTAKLFEASARLAAVLARAPAAIEEACAEYGKALGTAFQVIDDVLDYDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NSAEMGKNLGDDLREGKVTLPLIIAMQRGDAAQRQLIETAIQQGSLDELPAILAIVKATG
CHHHHHCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALEATRAAAAAQAQEAIRALSALPSNKYTEGLLQLASQLLDRRV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHGRSYNEPFTSQTALALPAQPHTSAILALIADDMHEVDKVIGLRLQSSVPLIGEIARY
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IISAGGKRLRPALLLLMSGALGYQEAQRFNLAAVIEFIHTATLLHDDVVDESTMRRGRPT
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCC
ANENFGNPASVLVGDFLHSRAFQMMVDADDMRVMQILSEATNIIAEGEVQQLMNTHDATL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
DEQGYLEVIRSKTAKLFEASARLAAVLARAPAAIEEACAEYGKALGTAFQVIDDVLDYDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NSAEMGKNLGDDLREGKVTLPLIIAMQRGDAAQRQLIETAIQQGSLDELPAILAIVKATG
CHHHHHCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALEATRAAAAAQAQEAIRALSALPSNKYTEGLLQLASQLLDRRV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]