Click here to switch to the map view.

The map label for this gene is ubiE [H]

Identifier: 120612289

GI number: 120612289

Start: 4017884

End: 4018615

Strand: Reverse

Name: ubiE [H]

Synonym: Aave_3644

Alternate gene names: 120612289

Gene position: 4018615-4017884 (Counterclockwise)

Preceding gene: 120612290

Following gene: 120612288

Centisome position: 75.08

GC content: 64.75

Gene sequence:

>732_bases
ATGAGCACCACCCATTTCGGCTTCCAGACCGTGGACGAGAACGACAAGGCGCGCCGCGTGCGCGGGGTTTTCGATTCCGT
CGCTTCCAAGTACGACGTCATGAACGACCTCATGTCGGGCGGCCTTCACCGCGCGTGGAAGGCCTATACCGTCATGGTCG
CCAACCTGCGCGAGGGCCAGCGCGTGCTGGACATCGCCGGCGGCACGGGCGACCTGGCGCGCGCGTTCGCGAAGAAGGTG
GGCGCGAGCGGCACGGTGGTGCACACCGACATCAACGAATCCATGCTGCGCGTGGGGCGCGACCGCCTCATCGACGAAGG
CACCGTGCTGCCCACGCTGGTCTGCGACGCCGAGCGGCTGCCGTTCCCCGACAACCATTTCGACGTGGTGAGCGTGGCCT
TCGGCCTGCGCAACATGACCCACAAGGACGTGGCGATCGCCGAGATGTGCCGCGTGCTCAAGCCGCGCGGCCGGCTGCTG
GTGCTGGAGTTCTCGCAGGTGGCCAAGCCGCTGCGCAAGCCCTACGACTGGTATTCCTTCAAGGTGCTGCCGCGGCTCGG
GAAGCTCGTGGCCGGCGACGATGCGAGCTACCGCTACCTGGCCGAGTCGATCCGCATGCATCCGGGCCAGGAAGAACTCA
AAACCCTCATGCAGAAAAATGGCTTTGGGCATGTGGACTATCACAACATGACTGGCGGTATCGTGGCCCTGCATGTTGGA
ATCAAGTGCTGA

Upstream 100 bases:

>100_bases
TTTTTCTTCCCATCCACGCGTACCCGCCGCGCGACACATGCCCCAACCCGTTGCGGGGTTGTCGCTGCCTGCGCGGCCTT
CGCACTCTTAGCATCCCACC

Downstream 100 bases:

>100_bases
ACTGGACGAAGCGCACGCTTTCGATAACTAAACCGAGTGGAGATGACATGATGAAATTCTGGTCTGTGGCTTTGGTGGCG
ATGCTCGCGGTGGTGCACCT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSTTHFGFQTVDENDKARRVRGVFDSVASKYDVMNDLMSGGLHRAWKAYTVMVANLREGQRVLDIAGGTGDLARAFAKKV
GASGTVVHTDINESMLRVGRDRLIDEGTVLPTLVCDAERLPFPDNHFDVVSVAFGLRNMTHKDVAIAEMCRVLKPRGRLL
VLEFSQVAKPLRKPYDWYSFKVLPRLGKLVAGDDASYRYLAESIRMHPGQEELKTLMQKNGFGHVDYHNMTGGIVALHVG
IKC

Sequences:

>Translated_243_residues
MSTTHFGFQTVDENDKARRVRGVFDSVASKYDVMNDLMSGGLHRAWKAYTVMVANLREGQRVLDIAGGTGDLARAFAKKV
GASGTVVHTDINESMLRVGRDRLIDEGTVLPTLVCDAERLPFPDNHFDVVSVAFGLRNMTHKDVAIAEMCRVLKPRGRLL
VLEFSQVAKPLRKPYDWYSFKVLPRLGKLVAGDDASYRYLAESIRMHPGQEELKTLMQKNGFGHVDYHNMTGGIVALHVG
IKC
>Mature_242_residues
STTHFGFQTVDENDKARRVRGVFDSVASKYDVMNDLMSGGLHRAWKAYTVMVANLREGQRVLDIAGGTGDLARAFAKKVG
ASGTVVHTDINESMLRVGRDRLIDEGTVLPTLVCDAERLPFPDNHFDVVSVAFGLRNMTHKDVAIAEMCRVLKPRGRLLV
LEFSQVAKPLRKPYDWYSFKVLPRLGKLVAGDDASYRYLAESIRMHPGQEELKTLMQKNGFGHVDYHNMTGGIVALHVGI
KC

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=44.7653429602888, Blast_Score=228, Evalue=3e-60,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=60.4938271604938, Blast_Score=292, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17557039, Length=243, Percent_Identity=45.2674897119342, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323526, Length=259, Percent_Identity=38.996138996139, Blast_Score=162, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24641702, Length=256, Percent_Identity=43.75, Blast_Score=217, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27065; Mature: 26933

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTHFGFQTVDENDKARRVRGVFDSVASKYDVMNDLMSGGLHRAWKAYTVMVANLREGQ
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
RVLDIAGGTGDLARAFAKKVGASGTVVHTDINESMLRVGRDRLIDEGTVLPTLVCDAERL
EEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHEEECCCCC
PFPDNHFDVVSVAFGLRNMTHKDVAIAEMCRVLKPRGRLLVLEFSQVAKPLRKPYDWYSF
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHE
KVLPRLGKLVAGDDASYRYLAESIRMHPGQEELKTLMQKNGFGHVDYHNMTGGIVALHVG
EHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEECCCCCCEEEEEEC
IKC
CCC
>Mature Secondary Structure 
STTHFGFQTVDENDKARRVRGVFDSVASKYDVMNDLMSGGLHRAWKAYTVMVANLREGQ
CCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
RVLDIAGGTGDLARAFAKKVGASGTVVHTDINESMLRVGRDRLIDEGTVLPTLVCDAERL
EEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHEEECCCCC
PFPDNHFDVVSVAFGLRNMTHKDVAIAEMCRVLKPRGRLLVLEFSQVAKPLRKPYDWYSF
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHE
KVLPRLGKLVAGDDASYRYLAESIRMHPGQEELKTLMQKNGFGHVDYHNMTGGIVALHVG
EHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEECCCCCCEEEEEEC
IKC
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA