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The map label for this gene is nudB [H]

Identifier: 120612281

GI number: 120612281

Start: 4008490

End: 4009014

Strand: Reverse

Name: nudB [H]

Synonym: Aave_3635

Alternate gene names: 120612281

Gene position: 4009014-4008490 (Counterclockwise)

Preceding gene: 120612282

Following gene: 120612279

Centisome position: 74.9

GC content: 71.05

Gene sequence:

>525_bases
ATGCGCCAGGGATACGTGCGGCCCCAGGCACCTCCATCCCGCGGCGTCGCGGGCTACAAGATTCCCGAGTCGGTGCTGGT
GGTGATCCATACGCCTGCCCTCGAGGTGCTGATGATCCGCCGCGCAGGCGGCGGGGACCCGCACTGGCAGTCCGTCACGG
GCAGCAAGGACTGGCCGGAGGAGCCCTTCGAGGCCGCCGCCGTCCGCGAGGTGTTCGAGGAGACCGGGATCGATGCCCGG
GCGCCAGGGCATGTGCTGACGGACTGGTTTCTCGAGAACGAGTACGACATCTGGCCCCAATGGCTGTACCGCTATGCGCC
GGGCGTGGTGCGCAACCGCGAGCGGCTGTTCGGGCTGCGGGTGCCCGCGGGCACGCCGGTGGCGCTGAACCCGCGCGAAC
ATGACGCCTGGCGGTGGCTGCCCTGGCGCGAGGCCGCGCTGGCCTGTTTCTCGCCGTCGAATGCGGAGGCCTGCCTGTGG
CTTCCGCGGTTCGCGGCCTGCGCGAGCCCGGAAGACCCCGGTTGA

Upstream 100 bases:

>100_bases
CCTTTCCATGGCATCGGGGAGCGGGTGTGTCCCGTGGTGGGTATGCCCTCTCTCGGGGCGGGCCGGCGCTGGACCTGGCG
GCTTTGATGGCGGCTGGCTG

Downstream 100 bases:

>100_bases
CCTCGGGCGGAGAGACCCGCGCCGCTGGCGGATCGTGGCGCCAGGCGCTCAGACAGGGCGCAGCCGCAGCATGTCGGCGG
CATCTCCGCCATGGGAGGCG

Product: NUDIX hydrolase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MRQGYVRPQAPPSRGVAGYKIPESVLVVIHTPALEVLMIRRAGGGDPHWQSVTGSKDWPEEPFEAAAVREVFEETGIDAR
APGHVLTDWFLENEYDIWPQWLYRYAPGVVRNRERLFGLRVPAGTPVALNPREHDAWRWLPWREAALACFSPSNAEACLW
LPRFAACASPEDPG

Sequences:

>Translated_174_residues
MRQGYVRPQAPPSRGVAGYKIPESVLVVIHTPALEVLMIRRAGGGDPHWQSVTGSKDWPEEPFEAAAVREVFEETGIDAR
APGHVLTDWFLENEYDIWPQWLYRYAPGVVRNRERLFGLRVPAGTPVALNPREHDAWRWLPWREAALACFSPSNAEACLW
LPRFAACASPEDPG
>Mature_174_residues
MRQGYVRPQAPPSRGVAGYKIPESVLVVIHTPALEVLMIRRAGGGDPHWQSVTGSKDWPEEPFEAAAVREVFEETGIDAR
APGHVLTDWFLENEYDIWPQWLYRYAPGVVRNRERLFGLRVPAGTPVALNPREHDAWRWLPWREAALACFSPSNAEACLW
LPRFAACASPEDPG

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=140, Percent_Identity=41.4285714285714, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 19599; Mature: 19599

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQGYVRPQAPPSRGVAGYKIPESVLVVIHTPALEVLMIRRAGGGDPHWQSVTGSKDWPE
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCC
EPFEAAAVREVFEETGIDARAPGHVLTDWFLENEYDIWPQWLYRYAPGVVRNRERLFGLR
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCHHHHHHHCCCHHCCHHHEEEEE
VPAGTPVALNPREHDAWRWLPWREAALACFSPSNAEACLWLPRFAACASPEDPG
CCCCCCEEECCCCCCCEECCCHHHHEEEEECCCCCCEEEECCHHHHHCCCCCCC
>Mature Secondary Structure
MRQGYVRPQAPPSRGVAGYKIPESVLVVIHTPALEVLMIRRAGGGDPHWQSVTGSKDWPE
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCC
EPFEAAAVREVFEETGIDARAPGHVLTDWFLENEYDIWPQWLYRYAPGVVRNRERLFGLR
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCHHHHHHHCCCHHCCHHHEEEEE
VPAGTPVALNPREHDAWRWLPWREAALACFSPSNAEACLWLPRFAACASPEDPG
CCCCCCEEECCCCCCCEECCCHHHHEEEEECCCCCCEEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]