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The map label for this gene is gsiD [H]

Identifier: 120612262

GI number: 120612262

Start: 3987613

End: 3988500

Strand: Direct

Name: gsiD [H]

Synonym: Aave_3615

Alternate gene names: 120612262

Gene position: 3987613-3988500 (Clockwise)

Preceding gene: 120612261

Following gene: 120612263

Centisome position: 74.5

GC content: 72.64

Gene sequence:

>888_bases
ATGCGCACGCCGCTCCGCGCCCCCGAGCCTGCCGCCACCCGGCCCGCCATGGCAGCGGACGGGCTGCGTCGCGCCCTGCG
GCATCCCAGCTTCGCCCTCGGCGCCGCGCTGACACTGGTGATGGTGCTGGCGGCGGGGCTGTCCTTCGCGTGGACGCCCG
GGTCGCCCTACGACATGGACATGGGATCGGCCCTCCTGCCGCCCGGAGCCGCGCACTGGCTGGGCACGGATGCGTATGGA
CGCGACGTGGCCTCACTGCTGCTCGTGGGCGCGCGCAATTCGATCCTGGTGGGCGTGATCGCGGTAGGCATCGGGCTCAC
GGCCGGCACGGCACTCGGGCTGCTGGCCGCGGCCCGGCGGGGCTGGGTCGAAGAAGCCGTGATGCGCCTGGCGGATTTCA
CATTCGCGTTTCCCGCGATCCTCTCGGCCATCATGCTGACCGCCGTCTGGGGTGCCGGCGTGGTGAATTCGATCATCGCC
ATCGGCATCTTCAACATCCCGACCTTCGCGCGCGTGGCGCGCGCCTCGGCCAAGGCTGTCTGGGCGCGGGAGTACGTGCT
GGCGGCGCGCGCGTGCGGCAAGGGCCCCTGGCGCATCACGCTGGAGCATGTGCTGCCCAACATCGCCGCCGTGCTGACCG
TGCAGGCGACGATCCAGTTCGCGCTGGCGATCCTGGCCGAGGCCGCCCTCTCCTACCTGGGCCTGGGCACGCAGCCGCCG
CAGCCCTCCTGGGGCCGGATGCTGAGCGAGGCGCAGACGCTGATGTTCCAGGCGCCCCTGCTGGCCGTGTGGCCCGGGCT
GGCGATCGCGCTGGCGGTGCTGGGATTGAACCTGCTGGGCGACGGGCTGCGCGACCTGCTCGATCCACGCCTGGCGCGCC
GCCGCTGA

Upstream 100 bases:

>100_bases
ACTGCGTGATGCTGCTGGCGGCCATGGTGATCGTGGTGAACTTCGCCGTGGACCTGCTCTACGCGGCGATCGATCCCCGC
GTGAAAGCGAGCGGTCTCTG

Downstream 100 bases:

>100_bases
ACGAAGAAGGTCCGCCAGTGACTTCCGGGAAGTTCCTCCCCCCCGCCCCTTCAACCACTCCGGGCGCCCTGCTCGAGGTG
CGCGGCCTCCAGGTGGACCT

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRTPLRAPEPAATRPAMAADGLRRALRHPSFALGAALTLVMVLAAGLSFAWTPGSPYDMDMGSALLPPGAAHWLGTDAYG
RDVASLLLVGARNSILVGVIAVGIGLTAGTALGLLAAARRGWVEEAVMRLADFTFAFPAILSAIMLTAVWGAGVVNSIIA
IGIFNIPTFARVARASAKAVWAREYVLAARACGKGPWRITLEHVLPNIAAVLTVQATIQFALAILAEAALSYLGLGTQPP
QPSWGRMLSEAQTLMFQAPLLAVWPGLAIALAVLGLNLLGDGLRDLLDPRLARRR

Sequences:

>Translated_295_residues
MRTPLRAPEPAATRPAMAADGLRRALRHPSFALGAALTLVMVLAAGLSFAWTPGSPYDMDMGSALLPPGAAHWLGTDAYG
RDVASLLLVGARNSILVGVIAVGIGLTAGTALGLLAAARRGWVEEAVMRLADFTFAFPAILSAIMLTAVWGAGVVNSIIA
IGIFNIPTFARVARASAKAVWAREYVLAARACGKGPWRITLEHVLPNIAAVLTVQATIQFALAILAEAALSYLGLGTQPP
QPSWGRMLSEAQTLMFQAPLLAVWPGLAIALAVLGLNLLGDGLRDLLDPRLARRR
>Mature_295_residues
MRTPLRAPEPAATRPAMAADGLRRALRHPSFALGAALTLVMVLAAGLSFAWTPGSPYDMDMGSALLPPGAAHWLGTDAYG
RDVASLLLVGARNSILVGVIAVGIGLTAGTALGLLAAARRGWVEEAVMRLADFTFAFPAILSAIMLTAVWGAGVVNSIIA
IGIFNIPTFARVARASAKAVWAREYVLAARACGKGPWRITLEHVLPNIAAVLTVQATIQFALAILAEAALSYLGLGTQPP
QPSWGRMLSEAQTLMFQAPLLAVWPGLAIALAVLGLNLLGDGLRDLLDPRLARRR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=272, Percent_Identity=43.3823529411765, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1787760, Length=289, Percent_Identity=35.2941176470588, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789889, Length=245, Percent_Identity=37.5510204081633, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1789964, Length=247, Percent_Identity=42.1052631578947, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787549, Length=239, Percent_Identity=35.9832635983264, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787498, Length=274, Percent_Identity=33.2116788321168, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788505, Length=231, Percent_Identity=29.8701298701299, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30923; Mature: 30923

Theoretical pI: Translated: 11.15; Mature: 11.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPLRAPEPAATRPAMAADGLRRALRHPSFALGAALTLVMVLAAGLSFAWTPGSPYDMD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
MGSALLPPGAAHWLGTDAYGRDVASLLLVGARNSILVGVIAVGIGLTAGTALGLLAAARR
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWVEEAVMRLADFTFAFPAILSAIMLTAVWGAGVVNSIIAIGIFNIPTFARVARASAKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WAREYVLAARACGKGPWRITLEHVLPNIAAVLTVQATIQFALAILAEAALSYLGLGTQPP
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QPSWGRMLSEAQTLMFQAPLLAVWPGLAIALAVLGLNLLGDGLRDLLDPRLARRR
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MRTPLRAPEPAATRPAMAADGLRRALRHPSFALGAALTLVMVLAAGLSFAWTPGSPYDMD
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
MGSALLPPGAAHWLGTDAYGRDVASLLLVGARNSILVGVIAVGIGLTAGTALGLLAAARR
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWVEEAVMRLADFTFAFPAILSAIMLTAVWGAGVVNSIIAIGIFNIPTFARVARASAKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WAREYVLAARACGKGPWRITLEHVLPNIAAVLTVQATIQFALAILAEAALSYLGLGTQPP
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QPSWGRMLSEAQTLMFQAPLLAVWPGLAIALAVLGLNLLGDGLRDLLDPRLARRR
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]