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The map label for this gene is nusA [H]

Identifier: 120612038

GI number: 120612038

Start: 3740295

End: 3741779

Strand: Reverse

Name: nusA [H]

Synonym: Aave_3384

Alternate gene names: 120612038

Gene position: 3741779-3740295 (Counterclockwise)

Preceding gene: 120612039

Following gene: 120612037

Centisome position: 69.9

GC content: 65.72

Gene sequence:

>1485_bases
ATGAATCGCGAACTGTTGATGTTGGTTGAAGCCATTTCGCGCGAGAAGAACGTGGAGCGCGACGTGGTGTTCGGCGCCGT
GGAGCTGGCACTGGCCCAGGCCACCAAGAAGCTCTACCCGGGCGAGGTGGACATCCGCGTGGCGATCGACCGCGACAGCG
GCAACTACGAGACTTTCCGCCGCTGGCTCGTCGTGCCCGACGACGCCGGCCTGCAGAACCCGGACGCCGAGGAACTGCTG
ATGGACGCGCAGGAGCGCATCGCCGACATCGAGGTCGGCGAGTACATCGAGGAAGGCATCGAGTCCCTGCCCATCGGCCG
CATCGGCGCCATGGCCGCCAAGCAGGTCATCCTGCAGAAGATCCGCGACGCCGAGCGCGAGATGCTGCTCAACGACTTCA
TGTCGCGCGGCGAGAAGATCTTCACCGGCACGGTCAAGCGCATGGACAAGGGCGACATCATCGTCGAGTCCGGCCGCGTC
GAAGGCCGCCTGCGCCGCAGCGAGATGATCCCCAAGGAAAACCTGCGCAACGGCGACCGCGTGCGGGCGATGATCATGGA
GGTGGACCTCACGCTGCGCGGCGCGCCCATCATCCTCTCGCGCTCCGCGCCCGAGTTCATGATCGAGCTCTTCCGCAACG
AGGTGCCCGAGATCGAACAGGGCCTGCTGGAGATCAAGAGCTGCGCCCGCGACCCCGGCAGCCGCGCCAAGATCGCCGTG
CTGTCGCACGACAAGCGCGTGGACCCCATCGGCACCTGCGTCGGCGTGCGCGGCACGCGCGTCAACGCCGTGACCAACGA
GCTGGCCGGCGAGCGCGTGGACATCGTGCTGTGGTCCGAGGACCCGGCGCAGTTCGTGATCGGCGCGCTGGCGCCGGCCA
ACGTGTCCTCCATCGTGGTCGATGAGGAAAAGCACGCCATGGACGTGGTGGTGGACGAGGAGAACCTCGCGATCGCCATC
GGCCGCGGCGGCCAGAACGTGCGCCTCGCTTCCGACCTGACGGGCTGGAAGATCAACATCATGGATGCGGCCGAGTCCGC
CCAGAAGCAGGCCAACGAGACCGATGCGTCCCGCCGCCTGTTCATGGAGAAGCTCGACGTGGACGAGGAAATCGCCAACA
TCCTCATCGAGGAAGGCTTCTCCAGCCTCGAGGAAGTGGCCTACGTGCCGCTGCAGGAGATGCTCGAGATCGAGAGCTTC
GACGAGGACACGGTGAACGAACTGCGCGCCCGCGCCAAGGATGCCCTCCTGACCATGGAGATCGCCCGCGAGGAAAGCGT
GGAAGGCGTCTCGCAGAACCTGCGGGACCTGGACGGGATGACGTCCGAGCTCATCGGCAAGCTGGCCGAGGGCGGTGTCC
ATACCCGTGATGATCTGGCCGACCTGGCCATCGACGAACTGACCGCCCTGACCGGCCAGTCTGAGGAAGAAGCCAAGGCC
TTGATCATGAAGGCGCGCGAACACTGGTTCGCGGGGCAAGAGTAA

Upstream 100 bases:

>100_bases
CGGGCCGGCCCCGGACTGAATCGGGCCGCAGCGGATTCGAAGCAAGGGCGGCCGCGCAGGGCGCGGGCCTTCAAGGGATT
GAAAAATAGGAGAGTCGTCG

Downstream 100 bases:

>100_bases
GGGTCAGGGGAGGTACGAACCGAATATGTCCAGTAACACTGTCGCCGAGTTCGCCAGCGAACTCAAGAAATCGCCGGAAA
CCCTGCTCGACCAGCTCAAG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNRELLMLVEAISREKNVERDVVFGAVELALAQATKKLYPGEVDIRVAIDRDSGNYETFRRWLVVPDDAGLQNPDAEELL
MDAQERIADIEVGEYIEEGIESLPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
EGRLRRSEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDEENLAIAI
GRGGQNVRLASDLTGWKINIMDAAESAQKQANETDASRRLFMEKLDVDEEIANILIEEGFSSLEEVAYVPLQEMLEIESF
DEDTVNELRARAKDALLTMEIAREESVEGVSQNLRDLDGMTSELIGKLAEGGVHTRDDLADLAIDELTALTGQSEEEAKA
LIMKAREHWFAGQE

Sequences:

>Translated_494_residues
MNRELLMLVEAISREKNVERDVVFGAVELALAQATKKLYPGEVDIRVAIDRDSGNYETFRRWLVVPDDAGLQNPDAEELL
MDAQERIADIEVGEYIEEGIESLPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
EGRLRRSEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDEENLAIAI
GRGGQNVRLASDLTGWKINIMDAAESAQKQANETDASRRLFMEKLDVDEEIANILIEEGFSSLEEVAYVPLQEMLEIESF
DEDTVNELRARAKDALLTMEIAREESVEGVSQNLRDLDGMTSELIGKLAEGGVHTRDDLADLAIDELTALTGQSEEEAKA
LIMKAREHWFAGQE
>Mature_494_residues
MNRELLMLVEAISREKNVERDVVFGAVELALAQATKKLYPGEVDIRVAIDRDSGNYETFRRWLVVPDDAGLQNPDAEELL
MDAQERIADIEVGEYIEEGIESLPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
EGRLRRSEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDEENLAIAI
GRGGQNVRLASDLTGWKINIMDAAESAQKQANETDASRRLFMEKLDVDEEIANILIEEGFSSLEEVAYVPLQEMLEIESF
DEDTVNELRARAKDALLTMEIAREESVEGVSQNLRDLDGMTSELIGKLAEGGVHTRDDLADLAIDELTALTGQSEEEAKA
LIMKAREHWFAGQE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=51.2145748987854, Blast_Score=479, Evalue=1e-136,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54894; Mature: 54894

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRELLMLVEAISREKNVERDVVFGAVELALAQATKKLYPGEVDIRVAIDRDSGNYETFR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
RWLVVPDDAGLQNPDAEELLMDAQERIADIEVGEYIEEGIESLPIGRIGAMAAKQVILQK
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRVEGRLRRSEMIPKENLRNGDR
HHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEECCCCCHHHHHHHCCCHHHCCCCHH
VRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
HEEEEEEECEEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEE
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVV
EECCCCCCHHHHEECCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCCEEEE
DEEKHAMDVVVDEENLAIAIGRGGQNVRLASDLTGWKINIMDAAESAQKQANETDASRRL
ECCCCCEEEEEECCCEEEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCCHHHHHH
FMEKLDVDEEIANILIEEGFSSLEEVAYVPLQEMLEIESFDEDTVNELRARAKDALLTME
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IAREESVEGVSQNLRDLDGMTSELIGKLAEGGVHTRDDLADLAIDELTALTGQSEEEAKA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LIMKAREHWFAGQE
HHHHHHHHHCCCCC
>Mature Secondary Structure
MNRELLMLVEAISREKNVERDVVFGAVELALAQATKKLYPGEVDIRVAIDRDSGNYETFR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
RWLVVPDDAGLQNPDAEELLMDAQERIADIEVGEYIEEGIESLPIGRIGAMAAKQVILQK
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRVEGRLRRSEMIPKENLRNGDR
HHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEECCCCCHHHHHHHCCCHHHCCCCHH
VRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
HEEEEEEECEEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEE
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVV
EECCCCCCHHHHEECCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCCEEEE
DEEKHAMDVVVDEENLAIAIGRGGQNVRLASDLTGWKINIMDAAESAQKQANETDASRRL
ECCCCCEEEEEECCCEEEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCCHHHHHH
FMEKLDVDEEIANILIEEGFSSLEEVAYVPLQEMLEIESFDEDTVNELRARAKDALLTME
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IAREESVEGVSQNLRDLDGMTSELIGKLAEGGVHTRDDLADLAIDELTALTGQSEEEAKA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LIMKAREHWFAGQE
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]