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The map label for this gene is phaD [H]

Identifier: 120611947

GI number: 120611947

Start: 3639792

End: 3641597

Strand: Direct

Name: phaD [H]

Synonym: Aave_3291

Alternate gene names: 120611947

Gene position: 3639792-3641597 (Clockwise)

Preceding gene: 120611946

Following gene: 120611948

Centisome position: 68.0

GC content: 69.21

Gene sequence:

>1806_bases
ATGAGTGCGGCCGCCCAGTTCTCGCAAGGGATGTCGGCGCTCATGCCGCACCTGATGCTCGCCCCCATCATGCTGCCGCT
GCTGACCGCGGCGCTCATGCTGCTGCTGCGCGAGGAGCGCCAGCGGCTGAAGCTGGGGATGAACCTGCTGTCCACGTTGG
CCAGCCTGTGCATATCGCTCGCGCTGCTGTTCTGGACGCACCTGGGCGGCAAGCCGGCCACGATGGGCGTGTACCTGCCT
GGCAACTGGCCCGCGCCTTTCGGCATCGTGCTCGCGCTGGACCGGCTGTCGGCGCTCATGCTCGTGCTCACGAGCTTCGT
CGCGCTGTGCTCCATCGTCTTCGCGGCGGCGCGCTGGCACCGTGCCGGCGTGCATTTCCACCCTCTGTTCCAGTTCCAGC
TCATGGGCCTGGCCGGGGCCTTCCTGACGGCGGACCTGTTCAACCTGTTCGTGTTCTTCGAGATCATGCTGGCCGCGTCG
TACGGGCTGCTGCTGCACGGTTCGGGGCGCCCGCGCGTGCAGGCGGGCCTGCATTACATCGCCATCAATCTGGCGGCATC
TTCGCTGTTCCTGATCGGCGTGTCGATGCTCTACGGCATCACGGGCACGCTGAACATGGCGGATCTGGCGCAGACCATTC
CCCACGTGGCCGATGCCGACCGGGGCCTGCTGCACGCGGCCGCGGGCATCCTGGCCACGGCCTTCCTCATCAAGGCGGCG
GTGTGGCCGCTGAATTTCTGGCTGGCGCCGGCCTACAGCGCGGCCAGCGCTCCTGCCGGGGCGCTGTTCGCGCTGATGAC
GAAAGTGGGCGTCTATACCATCCTGCGCCTGTGGACGCTGATGTTCGGCGCCGAGGCCGGGCCATCGGCGCTGTTCGGCA
GCCTGTGGCTCATAGGCGGCGGGCTCGTGACCATGGCGTTCGGTGCGATCGGCATGCTGGGCTCCCAGCGCATCGGCGTG
CTGGCCGGCTATGCGGCCATCCTGTCGTCGGGCACGCTGCTGGCGGCCGCGGGCTTCGGGCAGAACCTGCTGACGGCGGG
CCTGCTGTTCTACCTGCCCAGCTCCACGCTGGCCATCAGCGCCCTCTTCCTGCTCACCGACCTGATCGACCGCTGGCGCA
ACGACGGCTCCACCCTCGCGCCGCACGAACGGGACGATGATGCGCCCTTTCTCACACCCGAGTTGATCCCGGCGCAGCAA
ATGAACCTGGACGAGCAGGAGATGGTGCTGGTGGGCCGCGTCATCCCGGCGGCAGCGGCCCTCCTCGGCTTGGCTTTCCT
GCTGTGCACGCTCGTGATCGCGGGCCTGCCCCCGCTGTCGGGCTTCGTGGGCAAGTTCGCGATGCTCACGGCGCTGCTCA
ACCCTCTCGGATTGGGCCCGTCGGCCGGCGTCCGGGCATCGGCGGTGGGCTGGGTGCTGTTCGCGATGCTCATCGCCACC
GGGCTCATGGCCCTGCTCGCGCTCACCCGCACGGGCATGCGGCATTTCTGGACCACGCACGACCGCCCCGCTCCGCAATT
GCGCGTGCTGGAGGGCATTCCCATCGCGTTGCTGCTGGCCTGCTGCGTGGGCCTGACGCTGCAGGCTGGCCGGATGATGG
ATTTCACGCAGGCGACGGCGAACGCGCTGCACGCGCCGGGCGGCTATATCGATGCCGTGATGTCGGCCCGGCCGAAGCCC
GGCCCCGTCACGCCCGAAGGCACGGTCGCCATCGCACGCAGGTTGCCGCCTCCGGCTCCGTTGGCGGGCGTCCCGGCGGC
CGACGTCGCTGCGCGGCCTCCGGCCGGAACGAAGGTGATGCCATGA

Upstream 100 bases:

>100_bases
GACCATGTGGACGGATCGCATTCGCGCGACGTGCAGGAGATGCCATGACCGCCGCACGCGCCTTGCATCGCCACAGCCGC
CGCACGGCCGGGAGCCGCCG

Downstream 100 bases:

>100_bases
TGAAGCGCCTGTTTCCCGCGCCGCTGCTGTCCGTCGCCCTGGCCGGCATGTGGCTGCTGCTCAACCACTCGATGAGCGCA
GGCCATCTCATCCTCGCGGC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSAAAQFSQGMSALMPHLMLAPIMLPLLTAALMLLLREERQRLKLGMNLLSTLASLCISLALLFWTHLGGKPATMGVYLP
GNWPAPFGIVLALDRLSALMLVLTSFVALCSIVFAAARWHRAGVHFHPLFQFQLMGLAGAFLTADLFNLFVFFEIMLAAS
YGLLLHGSGRPRVQAGLHYIAINLAASSLFLIGVSMLYGITGTLNMADLAQTIPHVADADRGLLHAAAGILATAFLIKAA
VWPLNFWLAPAYSAASAPAGALFALMTKVGVYTILRLWTLMFGAEAGPSALFGSLWLIGGGLVTMAFGAIGMLGSQRIGV
LAGYAAILSSGTLLAAAGFGQNLLTAGLLFYLPSSTLAISALFLLTDLIDRWRNDGSTLAPHERDDDAPFLTPELIPAQQ
MNLDEQEMVLVGRVIPAAAALLGLAFLLCTLVIAGLPPLSGFVGKFAMLTALLNPLGLGPSAGVRASAVGWVLFAMLIAT
GLMALLALTRTGMRHFWTTHDRPAPQLRVLEGIPIALLLACCVGLTLQAGRMMDFTQATANALHAPGGYIDAVMSARPKP
GPVTPEGTVAIARRLPPPAPLAGVPAADVAARPPAGTKVMP

Sequences:

>Translated_601_residues
MSAAAQFSQGMSALMPHLMLAPIMLPLLTAALMLLLREERQRLKLGMNLLSTLASLCISLALLFWTHLGGKPATMGVYLP
GNWPAPFGIVLALDRLSALMLVLTSFVALCSIVFAAARWHRAGVHFHPLFQFQLMGLAGAFLTADLFNLFVFFEIMLAAS
YGLLLHGSGRPRVQAGLHYIAINLAASSLFLIGVSMLYGITGTLNMADLAQTIPHVADADRGLLHAAAGILATAFLIKAA
VWPLNFWLAPAYSAASAPAGALFALMTKVGVYTILRLWTLMFGAEAGPSALFGSLWLIGGGLVTMAFGAIGMLGSQRIGV
LAGYAAILSSGTLLAAAGFGQNLLTAGLLFYLPSSTLAISALFLLTDLIDRWRNDGSTLAPHERDDDAPFLTPELIPAQQ
MNLDEQEMVLVGRVIPAAAALLGLAFLLCTLVIAGLPPLSGFVGKFAMLTALLNPLGLGPSAGVRASAVGWVLFAMLIAT
GLMALLALTRTGMRHFWTTHDRPAPQLRVLEGIPIALLLACCVGLTLQAGRMMDFTQATANALHAPGGYIDAVMSARPKP
GPVTPEGTVAIARRLPPPAPLAGVPAADVAARPPAGTKVMP
>Mature_600_residues
SAAAQFSQGMSALMPHLMLAPIMLPLLTAALMLLLREERQRLKLGMNLLSTLASLCISLALLFWTHLGGKPATMGVYLPG
NWPAPFGIVLALDRLSALMLVLTSFVALCSIVFAAARWHRAGVHFHPLFQFQLMGLAGAFLTADLFNLFVFFEIMLAASY
GLLLHGSGRPRVQAGLHYIAINLAASSLFLIGVSMLYGITGTLNMADLAQTIPHVADADRGLLHAAAGILATAFLIKAAV
WPLNFWLAPAYSAASAPAGALFALMTKVGVYTILRLWTLMFGAEAGPSALFGSLWLIGGGLVTMAFGAIGMLGSQRIGVL
AGYAAILSSGTLLAAAGFGQNLLTAGLLFYLPSSTLAISALFLLTDLIDRWRNDGSTLAPHERDDDAPFLTPELIPAQQM
NLDEQEMVLVGRVIPAAAALLGLAFLLCTLVIAGLPPLSGFVGKFAMLTALLNPLGLGPSAGVRASAVGWVLFAMLIATG
LMALLALTRTGMRHFWTTHDRPAPQLRVLEGIPIALLLACCVGLTLQAGRMMDFTQATANALHAPGGYIDAVMSARPKPG
PVTPEGTVAIARRLPPPAPLAGVPAADVAARPPAGTKVMP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=398, Percent_Identity=27.3869346733668, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788827, Length=415, Percent_Identity=25.3012048192771, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 63073; Mature: 62942

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAAQFSQGMSALMPHLMLAPIMLPLLTAALMLLLREERQRLKLGMNLLSTLASLCISL
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLFWTHLGGKPATMGVYLPGNWPAPFGIVLALDRLSALMLVLTSFVALCSIVFAAARWH
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGVHFHPLFQFQLMGLAGAFLTADLFNLFVFFEIMLAASYGLLLHGSGRPRVQAGLHYI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCHHHH
AINLAASSLFLIGVSMLYGITGTLNMADLAQTIPHVADADRGLLHAAAGILATAFLIKAA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHH
VWPLNFWLAPAYSAASAPAGALFALMTKVGVYTILRLWTLMFGAEAGPSALFGSLWLIGG
HHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVTMAFGAIGMLGSQRIGVLAGYAAILSSGTLLAAAGFGQNLLTAGLLFYLPSSTLAIS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHH
ALFLLTDLIDRWRNDGSTLAPHERDDDAPFLTPELIPAQQMNLDEQEMVLVGRVIPAAAA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHH
LLGLAFLLCTLVIAGLPPLSGFVGKFAMLTALLNPLGLGPSAGVRASAVGWVLFAMLIAT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
GLMALLALTRTGMRHFWTTHDRPAPQLRVLEGIPIALLLACCVGLTLQAGRMMDFTQATA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
NALHAPGGYIDAVMSARPKPGPVTPEGTVAIARRLPPPAPLAGVPAADVAARPPAGTKVM
HHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCC
P
C
>Mature Secondary Structure 
SAAAQFSQGMSALMPHLMLAPIMLPLLTAALMLLLREERQRLKLGMNLLSTLASLCISL
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLFWTHLGGKPATMGVYLPGNWPAPFGIVLALDRLSALMLVLTSFVALCSIVFAAARWH
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGVHFHPLFQFQLMGLAGAFLTADLFNLFVFFEIMLAASYGLLLHGSGRPRVQAGLHYI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCHHHH
AINLAASSLFLIGVSMLYGITGTLNMADLAQTIPHVADADRGLLHAAAGILATAFLIKAA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHH
VWPLNFWLAPAYSAASAPAGALFALMTKVGVYTILRLWTLMFGAEAGPSALFGSLWLIGG
HHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVTMAFGAIGMLGSQRIGVLAGYAAILSSGTLLAAAGFGQNLLTAGLLFYLPSSTLAIS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHH
ALFLLTDLIDRWRNDGSTLAPHERDDDAPFLTPELIPAQQMNLDEQEMVLVGRVIPAAAA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHH
LLGLAFLLCTLVIAGLPPLSGFVGKFAMLTALLNPLGLGPSAGVRASAVGWVLFAMLIAT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
GLMALLALTRTGMRHFWTTHDRPAPQLRVLEGIPIALLLACCVGLTLQAGRMMDFTQATA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
NALHAPGGYIDAVMSARPKPGPVTPEGTVAIARRLPPPAPLAGVPAADVAARPPAGTKVM
HHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]