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The map label for this gene is rlmH

Identifier: 120611857

GI number: 120611857

Start: 3527835

End: 3528302

Strand: Reverse

Name: rlmH

Synonym: Aave_3199

Alternate gene names: 120611857

Gene position: 3528302-3527835 (Counterclockwise)

Preceding gene: 120611858

Following gene: 120611856

Centisome position: 65.92

GC content: 70.73

Gene sequence:

>468_bases
ATGAAGCTGCTCATCGTGGCCGTGGGCCAGCGCGTGCCCGACTGGGCGCAGACCGCCTACGAGGACTATGCCAAGCGGTT
CCCGCCCGAACTGAAGGTGGAACTCAAGGCCGTCAAGACCGAGCCGCGCGGCTCCAAGACGCTGGAAACCCTCTACGCCG
CCGAGCGCGAGCGCATCGAGGCCGCCATTCCGCGCGGCACGCGCGTGGTGGCGCTCGACGAGCGCGGCACCTCGCTCACC
ACCAAGGCCCTGGCCGCCCGCCTCAAGGACTGGCAGCTGGGCGGCGACGACGTGGCGCTGGTCATCGGCGGTCCGGACGG
GCTCGACCCGGCCTTCCGCCAGGCCGCGCACGAGCGCATCCGCCTCTCGGACCTCACGCTGCCGCATGCGATGGTGCGGG
TGCTGCTGATCGAGCAGCTCTACCGTGCGTGGTCCGTGAACGCCGGCCATCCCTACCACCGGGAATAG

Upstream 100 bases:

>100_bases
GGCGGCTCCGCGCAAGGCTGCCGCCAGGAAGGCCCCGTCCCGCAAGAAGGCCGGCTGAGTGAGCAGGCAGGCGGGCATGC
CGCCCGCGGAGCATTGACCG

Downstream 100 bases:

>100_bases
CCTCCGCGCAGCGGCGCCAGACCTGCATCGCATGCGTCGTTCCGCCGCAGGTTTCCCCCCGGGCAGCTCGCCCTGCAGCA
CGAAGCCCGCATGCCGCAGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKLLIVAVGQRVPDWAQTAYEDYAKRFPPELKVELKAVKTEPRGSKTLETLYAAERERIEAAIPRGTRVVALDERGTSLT
TKALAARLKDWQLGGDDVALVIGGPDGLDPAFRQAAHERIRLSDLTLPHAMVRVLLIEQLYRAWSVNAGHPYHRE

Sequences:

>Translated_155_residues
MKLLIVAVGQRVPDWAQTAYEDYAKRFPPELKVELKAVKTEPRGSKTLETLYAAERERIEAAIPRGTRVVALDERGTSLT
TKALAARLKDWQLGGDDVALVIGGPDGLDPAFRQAAHERIRLSDLTLPHAMVRVLLIEQLYRAWSVNAGHPYHRE
>Mature_155_residues
MKLLIVAVGQRVPDWAQTAYEDYAKRFPPELKVELKAVKTEPRGSKTLETLYAAERERIEAAIPRGTRVVALDERGTSLT
TKALAARLKDWQLGGDDVALVIGGPDGLDPAFRQAAHERIRLSDLTLPHAMVRVLLIEQLYRAWSVNAGHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=47.4358974358974, Blast_Score=142, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ACIAC (A1TS23)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971535.1
- ProteinModelPortal:   A1TS23
- SMR:   A1TS23
- STRING:   A1TS23
- GeneID:   4668944
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3199
- NMPDR:   fig|397945.5.peg.2733
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   A1TS23
- ProtClustDB:   PRK00103
- BioCyc:   AAVE397945:AAVE_3199-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17349; Mature: 17349

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLIVAVGQRVPDWAQTAYEDYAKRFPPELKVELKAVKTEPRGSKTLETLYAAERERIE
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHH
AAIPRGTRVVALDERGTSLTTKALAARLKDWQLGGDDVALVIGGPDGLDPAFRQAAHERI
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
RLSDLTLPHAMVRVLLIEQLYRAWSVNAGHPYHRE
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLLIVAVGQRVPDWAQTAYEDYAKRFPPELKVELKAVKTEPRGSKTLETLYAAERERIE
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHH
AAIPRGTRVVALDERGTSLTTKALAARLKDWQLGGDDVALVIGGPDGLDPAFRQAAHERI
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
RLSDLTLPHAMVRVLLIEQLYRAWSVNAGHPYHRE
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA