Click here to switch to the map view.

The map label for this gene is rlmE

Identifier: 120611284

GI number: 120611284

Start: 2866137

End: 2866793

Strand: Direct

Name: rlmE

Synonym: Aave_2616

Alternate gene names: 120611284

Gene position: 2866137-2866793 (Clockwise)

Preceding gene: 120611281

Following gene: 120611285

Centisome position: 53.54

GC content: 64.08

Gene sequence:

>657_bases
ATGAAAGCCAACACGAAAAGCAAGAAGGTGAACAAGGCGTGGCTCCACGACCACGTCAACGACACCTACGTCAAGCTCGC
CCAGAAAGAGGGCTACCGCGCACGTGCCGCCTACAAGCTCAAGGAAATCGACGAAACCCTCGGGCTCATTCGCCCGGGGC
AGGTGGTGGTCGATCTCGGCTCCGCGCCCGGTGCCTGGAGCCAGTACCTGCGCAGGCGCATGGCGCCCGCGGGCGCGGCC
GCCGGCCAGCTCAATGGCACCCTGATCGCGCTCGACATCCTCCCCATGGAGCCGATCGAGGGCGTCACCTTCCTGCAGGG
CGACTTCCGCGAGGAGGACGTGCTCGCGCGGCTCCAGGAGGCCGTGCAGGCGCGGCCGGTGGACGTGGTGGTGTCGGACA
TGGCGCCCAACCTGTCGGGGGTGGAATCGGTCGATGCCGTGCGCATCGCCCACCTGATCGAGCTGGCGGTGGATTTCGCG
CAGCAGCACCTCAAGCCGGACGGTGCGCTGGTAGTGAAACTCTTCCATGGCAGCGGATATGCCCAGCTGGTGCAGCTTTT
CAAGGAACACTTCCGCACCGTCAAACCGATGAAGCCCAAGGCATCGCGCGACAAATCGTCGGAAACCTTTCTCGTCGGAA
TGGGTTTGCGCAAATAA

Upstream 100 bases:

>100_bases
TCATCACCACGGGGTCCAGGTGGTGGGCGTTGGCGCGGTGTTCCCGGCGCTCCGCGGGGGTCAATTGGATTTGGGGCATG
GGCCGTATTATCGAGGCAGG

Downstream 100 bases:

>100_bases
CCCTGCGGGAATTTTTCCCGCCCCCGTGCAGGCGGCCATGCCGCGGGCGGGCCGGCCGGATGCCCGAGCTATGGTGACCA
TAGCCGCGGTTGGGGTAAAG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKANTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDETLGLIRPGQVVVDLGSAPGAWSQYLRRRMAPAGAA
AGQLNGTLIALDILPMEPIEGVTFLQGDFREEDVLARLQEAVQARPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFA
QQHLKPDGALVVKLFHGSGYAQLVQLFKEHFRTVKPMKPKASRDKSSETFLVGMGLRK

Sequences:

>Translated_218_residues
MKANTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDETLGLIRPGQVVVDLGSAPGAWSQYLRRRMAPAGAA
AGQLNGTLIALDILPMEPIEGVTFLQGDFREEDVLARLQEAVQARPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFA
QQHLKPDGALVVKLFHGSGYAQLVQLFKEHFRTVKPMKPKASRDKSSETFLVGMGLRK
>Mature_218_residues
MKANTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDETLGLIRPGQVVVDLGSAPGAWSQYLRRRMAPAGAA
AGQLNGTLIALDILPMEPIEGVTFLQGDFREEDVLARLQEAVQARPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFA
QQHLKPDGALVVKLFHGSGYAQLVQLFKEHFRTVKPMKPKASRDKSSETFLVGMGLRK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=200, Percent_Identity=37, Blast_Score=140, Evalue=7e-34,
Organism=Homo sapiens, GI29029591, Length=195, Percent_Identity=35.3846153846154, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI29029589, Length=195, Percent_Identity=35.3846153846154, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI7110661, Length=195, Percent_Identity=35.3846153846154, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI194097365, Length=196, Percent_Identity=31.6326530612245, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1789569, Length=202, Percent_Identity=46.039603960396, Blast_Score=184, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17554650, Length=194, Percent_Identity=33.5051546391753, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553474, Length=221, Percent_Identity=32.579185520362, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=33.160621761658, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=33.160621761658, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=33.160621761658, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=198, Percent_Identity=33.8383838383838, Blast_Score=115, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319535, Length=201, Percent_Identity=33.8308457711443, Blast_Score=115, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321302, Length=98, Percent_Identity=40.8163265306122, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356387, Length=210, Percent_Identity=36.1904761904762, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI18859957, Length=195, Percent_Identity=32.8205128205128, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=33.6787564766839, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24647580, Length=197, Percent_Identity=33.502538071066, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ACIAC (A1TQF0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970962.1
- ProteinModelPortal:   A1TQF0
- SMR:   A1TQF0
- STRING:   A1TQF0
- GeneID:   4668206
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2616
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A1TQF0
- ProtClustDB:   CLSK951379
- BioCyc:   AAVE397945:AAVE_2616-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24010; Mature: 24010

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: ACT_SITE 173-173 BINDING 64-64 BINDING 66-66 BINDING 92-92 BINDING 108-108 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKANTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDETLGLIRPGQVVVDLG
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECC
SAPGAWSQYLRRRMAPAGAAAGQLNGTLIALDILPMEPIEGVTFLQGDFREEDVLARLQE
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHH
AVQARPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFAQQHLKPDGALVVKLFHGSGY
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
AQLVQLFKEHFRTVKPMKPKASRDKSSETFLVGMGLRK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MKANTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDETLGLIRPGQVVVDLG
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECC
SAPGAWSQYLRRRMAPAGAAAGQLNGTLIALDILPMEPIEGVTFLQGDFREEDVLARLQE
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHH
AVQARPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFAQQHLKPDGALVVKLFHGSGY
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
AQLVQLFKEHFRTVKPMKPKASRDKSSETFLVGMGLRK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA