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The map label for this gene is bioB

Identifier: 120611187

GI number: 120611187

Start: 2759602

End: 2760666

Strand: Direct

Name: bioB

Synonym: Aave_2518

Alternate gene names: 120611187

Gene position: 2759602-2760666 (Clockwise)

Preceding gene: 120611186

Following gene: 120611191

Centisome position: 51.55

GC content: 69.58

Gene sequence:

>1065_bases
ATGACTGCCACTCTTTCCGATATCCGTCCCGAAGCCCCTGCCGTTCCCGTGCAACTGCACCGCCCCGCACGCACTCCTGC
CCCCGCGGCCGGCACCTGGAGCGTCCAGGCCGTGCAGGAACTGCTGGACCTGCCGTTCATGGAACTGCTCTGGCGCGCGC
AGTCCGTGCACCGTGAGCACTGGCCCGCGGGCGACGTCGAACTGGCCACGCTGCTGTCGGTGAAGACGGGCGGCTGCCCC
GAGAACTGCGGCTATTGCCCGCAGTCCGCCGAGTTCGACACGGGCGTGAAGGCGCAGAAGCTCATGAGCGTGGACGAGGT
GACCACCGCCGCCCGCGCCGCCCGCGACGCCGGTGCCACGCGCTTTTGCATGGGCGCCGCGTGGCGCGCGCCCAAGGACC
GCGACATCGAGAAGGTGGGCGAGCTGATCGCCGCCGTGAAGGGGCTCGGGATGCAGACCTGCGCCACCCTGGGCATGCTG
GAGCCGCACCAGGCCCAGGCGCTCAAGGGCGCGGGGCTGGACTACTACAACCACAACCTGGATACCGCGCCCGAGTACTA
CACCGATGTCGTGAGCACCCGCGCCTACCAGGAACGGCTCGATACCCTGCGCCATGTGCGCGAGGCCGGCATCAGCGTCT
GCTGCGGCGGCATCATCGGCATGGGGGAGGCACCGGTGCACCGCGCGGGCCTGATCGCCCAGCTCGCCAACCTCGATCCG
TACCCGGAATCCGTACCCATCAACAGCCTGGTGCGTGTGCCCGGCACGCCGCTGGCCGACAGCGATCCGGTCGATCCGCT
CGATTTCGTGCGGGTGATCGCGGTGGCGCGCATCACCATGCCGCGTGCGCGCGTGCGCCTCTCGGCGGGCCGCCAGCAAA
TGGGCGATGCGGTGCAGGCGCTGTGCTTCCTGGCCGGGGCCAATTCGATCTTCTATGGCGACAAGCTGCTGGTGACCGGC
AACCCGGACGTGGATGCCGACGTGCAACTGCTCGCCAAACTGGGGATGAACGGCCGGCAGGTGGCCTCCACCGAGCCCCA
CGACCTGCACCACCACGCCCCCTGA

Upstream 100 bases:

>100_bases
AGCGCGGGTTTTTGGCGGCAAAGGCTTGGCACGCGGCCCCGTTCTCTTCCAGAATTCAGCCCAAGCGCCCAGCGTGCACC
TTGTGTCCGTACCCACTGCC

Downstream 100 bases:

>100_bases
GAGCTGGCAGGAGGGCGATCACCGCCCGCCGCGCGGCGGTTCGTACCCGAGCATCTTCATGGCCTGCGTGATGGAGACCG
CCGCCTTGGCGTAGCCGTCC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTATLSDIRPEAPAVPVQLHRPARTPAPAAGTWSVQAVQELLDLPFMELLWRAQSVHREHWPAGDVELATLLSVKTGGCP
ENCGYCPQSAEFDTGVKAQKLMSVDEVTTAARAARDAGATRFCMGAAWRAPKDRDIEKVGELIAAVKGLGMQTCATLGML
EPHQAQALKGAGLDYYNHNLDTAPEYYTDVVSTRAYQERLDTLRHVREAGISVCCGGIIGMGEAPVHRAGLIAQLANLDP
YPESVPINSLVRVPGTPLADSDPVDPLDFVRVIAVARITMPRARVRLSAGRQQMGDAVQALCFLAGANSIFYGDKLLVTG
NPDVDADVQLLAKLGMNGRQVASTEPHDLHHHAP

Sequences:

>Translated_354_residues
MTATLSDIRPEAPAVPVQLHRPARTPAPAAGTWSVQAVQELLDLPFMELLWRAQSVHREHWPAGDVELATLLSVKTGGCP
ENCGYCPQSAEFDTGVKAQKLMSVDEVTTAARAARDAGATRFCMGAAWRAPKDRDIEKVGELIAAVKGLGMQTCATLGML
EPHQAQALKGAGLDYYNHNLDTAPEYYTDVVSTRAYQERLDTLRHVREAGISVCCGGIIGMGEAPVHRAGLIAQLANLDP
YPESVPINSLVRVPGTPLADSDPVDPLDFVRVIAVARITMPRARVRLSAGRQQMGDAVQALCFLAGANSIFYGDKLLVTG
NPDVDADVQLLAKLGMNGRQVASTEPHDLHHHAP
>Mature_353_residues
TATLSDIRPEAPAVPVQLHRPARTPAPAAGTWSVQAVQELLDLPFMELLWRAQSVHREHWPAGDVELATLLSVKTGGCPE
NCGYCPQSAEFDTGVKAQKLMSVDEVTTAARAARDAGATRFCMGAAWRAPKDRDIEKVGELIAAVKGLGMQTCATLGMLE
PHQAQALKGAGLDYYNHNLDTAPEYYTDVVSTRAYQERLDTLRHVREAGISVCCGGIIGMGEAPVHRAGLIAQLANLDPY
PESVPINSLVRVPGTPLADSDPVDPLDFVRVIAVARITMPRARVRLSAGRQQMGDAVQALCFLAGANSIFYGDKLLVTGN
PDVDADVQLLAKLGMNGRQVASTEPHDLHHHAP

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=310, Percent_Identity=58.3870967741935, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=46.0063897763578, Blast_Score=301, Evalue=8e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ACIAC (A1TQ53)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970865.1
- ProteinModelPortal:   A1TQ53
- SMR:   A1TQ53
- STRING:   A1TQ53
- GeneID:   4668820
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2518
- NMPDR:   fig|397945.5.peg.2156
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1TQ53
- ProtClustDB:   CLSK2321419
- BioCyc:   AAVE397945:AAVE_2518-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38006; Mature: 37875

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLSDIRPEAPAVPVQLHRPARTPAPAAGTWSVQAVQELLDLPFMELLWRAQSVHREH
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHC
WPAGDVELATLLSVKTGGCPENCGYCPQSAEFDTGVKAQKLMSVDEVTTAARAARDAGAT
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
RFCMGAAWRAPKDRDIEKVGELIAAVKGLGMQTCATLGMLEPHQAQALKGAGLDYYNHNL
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCCHHCCCC
DTAPEYYTDVVSTRAYQERLDTLRHVREAGISVCCGGIIGMGEAPVHRAGLIAQLANLDP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHCCCCCCHHHHHHHHHHHCCCC
YPESVPINSLVRVPGTPLADSDPVDPLDFVRVIAVARITMPRARVRLSAGRQQMGDAVQA
CCCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LCFLAGANSIFYGDKLLVTGNPDVDADVQLLAKLGMNGRQVASTEPHDLHHHAP
HHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
TATLSDIRPEAPAVPVQLHRPARTPAPAAGTWSVQAVQELLDLPFMELLWRAQSVHREH
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHC
WPAGDVELATLLSVKTGGCPENCGYCPQSAEFDTGVKAQKLMSVDEVTTAARAARDAGAT
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
RFCMGAAWRAPKDRDIEKVGELIAAVKGLGMQTCATLGMLEPHQAQALKGAGLDYYNHNL
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCCHHCCCC
DTAPEYYTDVVSTRAYQERLDTLRHVREAGISVCCGGIIGMGEAPVHRAGLIAQLANLDP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHCCCCCCHHHHHHHHHHHCCCC
YPESVPINSLVRVPGTPLADSDPVDPLDFVRVIAVARITMPRARVRLSAGRQQMGDAVQA
CCCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LCFLAGANSIFYGDKLLVTGNPDVDADVQLLAKLGMNGRQVASTEPHDLHHHAP
HHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA