Click here to switch to the map view.

The map label for this gene is hdhA [C]

Identifier: 120610932

GI number: 120610932

Start: 2484861

End: 2485676

Strand: Reverse

Name: hdhA [C]

Synonym: Aave_2258

Alternate gene names: 120610932

Gene position: 2485676-2484861 (Counterclockwise)

Preceding gene: 120610935

Following gene: 120610930

Centisome position: 46.44

GC content: 71.45

Gene sequence:

>816_bases
ATGTCTCAGATTTCACCCGTTCCTTCTTCCGGCGCCGCCGCCTCGCCGTCCGGAGCCCTGTCGGTGTTTCCCAGCCTGCG
CGGACGCGTGGTGTTCGTGACGGGTGGCGGCAGCGGGATCGGCGCCGCCATCGTCACGGCCTTCGCCGAGCAGGGCGCGC
GCGTGGCGTTCGTGGACGTGGCCCGCGAGGCCAGCGGGCAGCTGGTCCGCGACATGGAGGCCGCGGGCCTGCCGCGCCCC
TGGTGGCGCGAGTGCGACGTGCGCGACATCGATGCCCTGCGCGCCGCCCTGCGCGATGCCGCGGCCGAGCTGGGCGATGT
CTCCGTGCTGGTCAACAACGTGGCCAGCGACGACCGCCACACGCTGGAGTCCGTCACCCCCGAGTACTATGACGAACGCA
TGGCGATCAACGAGCGCCCGGCCTTCTTCGCGATCCAGGCGGTGGTGCCGGGCATGCGCCGGCTCGGCGGCGGCTCCATC
GTCAACCTCGGCTCCACCGGCTGGCAGGGCAAGGGGTCGGGCTACCCCTGCTATGCGATCGCCAAGTCGTCGGTGAACGG
GCTCACCCGGGGCCTGGCGCGCACGCTGGGCCAGGACCGCATCCGCATCAACACCGTGTCCCCCGGCTGGGTGATGACCG
AGCGCCAGATCCGGCTGTGGCTCGACGCCGAGGGCGAGCGGGAACTCGCGCGCAACCAGTGCCTGCCCGACAAGCTGCAG
CCGCGCGACATCGCGCGCATGGTGCTGTTCCTGGGCTCCGACGACGCCGCGATGTGCACCGCGCAGGAGTTCAAGGTGGA
CGCTGGATGGGTCTAG

Upstream 100 bases:

>100_bases
AGCGCCCGTCTGTCGCGGGCCTCTTGACGCGGCGGATCTTATCAACGCAAATTGATGCACGTAACCATTATTTTTTCTAT
CAATCGATACTGAAATTTAT

Downstream 100 bases:

>100_bases
CAGAAAGCAACCCCCTGAGGCGCTGGCGCGCCTTCCCCCTTTCTCTCGCCGGGCTGCGCCCGGCGGGAAGGGGGACACCG
CCAGCGCGGCGGGGCGGCCC

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSQISPVPSSGAAASPSGALSVFPSLRGRVVFVTGGGSGIGAAIVTAFAEQGARVAFVDVAREASGQLVRDMEAAGLPRP
WWRECDVRDIDALRAALRDAAAELGDVSVLVNNVASDDRHTLESVTPEYYDERMAINERPAFFAIQAVVPGMRRLGGGSI
VNLGSTGWQGKGSGYPCYAIAKSSVNGLTRGLARTLGQDRIRINTVSPGWVMTERQIRLWLDAEGERELARNQCLPDKLQ
PRDIARMVLFLGSDDAAMCTAQEFKVDAGWV

Sequences:

>Translated_271_residues
MSQISPVPSSGAAASPSGALSVFPSLRGRVVFVTGGGSGIGAAIVTAFAEQGARVAFVDVAREASGQLVRDMEAAGLPRP
WWRECDVRDIDALRAALRDAAAELGDVSVLVNNVASDDRHTLESVTPEYYDERMAINERPAFFAIQAVVPGMRRLGGGSI
VNLGSTGWQGKGSGYPCYAIAKSSVNGLTRGLARTLGQDRIRINTVSPGWVMTERQIRLWLDAEGERELARNQCLPDKLQ
PRDIARMVLFLGSDDAAMCTAQEFKVDAGWV
>Mature_270_residues
SQISPVPSSGAAASPSGALSVFPSLRGRVVFVTGGGSGIGAAIVTAFAEQGARVAFVDVAREASGQLVRDMEAAGLPRPW
WRECDVRDIDALRAALRDAAAELGDVSVLVNNVASDDRHTLESVTPEYYDERMAINERPAFFAIQAVVPGMRRLGGGSIV
NLGSTGWQGKGSGYPCYAIAKSSVNGLTRGLARTLGQDRIRINTVSPGWVMTERQIRLWLDAEGERELARNQCLPDKLQP
RDIARMVLFLGSDDAAMCTAQEFKVDAGWV

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=245, Percent_Identity=30.2040816326531, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7705925, Length=246, Percent_Identity=32.1138211382114, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI126723750, Length=248, Percent_Identity=27.8225806451613, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=31.640625, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI66933014, Length=251, Percent_Identity=29.8804780876494, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI19923817, Length=253, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40254992, Length=247, Percent_Identity=28.7449392712551, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=29.4573643410853, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI31542939, Length=195, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=29.1208791208791, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI20149619, Length=231, Percent_Identity=26.4069264069264, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=30.0395256916996, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789208, Length=254, Percent_Identity=29.1338582677165, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082100, Length=251, Percent_Identity=27.8884462151394, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=25.4032258064516, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786812, Length=253, Percent_Identity=29.2490118577075, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=29.8387096774194, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789378, Length=274, Percent_Identity=27.007299270073, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082160, Length=255, Percent_Identity=23.921568627451, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=28.2868525896414, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367175, Length=236, Percent_Identity=26.271186440678, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=25.3012048192771, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787820, Length=183, Percent_Identity=26.775956284153, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=27.710843373494, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567345, Length=193, Percent_Identity=29.5336787564767, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=29.8804780876494, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=241, Percent_Identity=28.6307053941909, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=256, Percent_Identity=32.03125, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=257, Percent_Identity=26.8482490272374, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=243, Percent_Identity=28.8065843621399, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=25.7936507936508, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=190, Percent_Identity=24.7368421052632, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=194, Percent_Identity=23.7113402061856, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=248, Percent_Identity=25.4032258064516, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=254, Percent_Identity=25.5905511811024, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=247, Percent_Identity=25.1012145748988, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=251, Percent_Identity=24.7011952191235, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=254, Percent_Identity=27.9527559055118, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=241, Percent_Identity=28.2157676348548, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=240, Percent_Identity=27.0833333333333, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=205, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=248, Percent_Identity=25.8064516129032, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=27.0588235294118, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=254, Percent_Identity=25.9842519685039, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=27.4900398406374, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24644339, Length=247, Percent_Identity=29.1497975708502, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571526, Length=249, Percent_Identity=26.5060240963855, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=28.9156626506024, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=26.5060240963855, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=28.8537549407115, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281364892, Length=186, Percent_Identity=30.6451612903226, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21355319, Length=242, Percent_Identity=24.3801652892562, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=257, Percent_Identity=29.5719844357977, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 29008; Mature: 28877

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQISPVPSSGAAASPSGALSVFPSLRGRVVFVTGGGSGIGAAIVTAFAEQGARVAFVDV
CCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEH
AREASGQLVRDMEAAGLPRPWWRECDVRDIDALRAALRDAAAELGDVSVLVNNVASDDRH
HHHHCCHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHH
TLESVTPEYYDERMAINERPAFFAIQAVVPGMRRLGGGSIVNLGSTGWQGKGSGYPCYAI
HHHHCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEE
AKSSVNGLTRGLARTLGQDRIRINTVSPGWVMTERQIRLWLDAEGERELARNQCLPDKLQ
HHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCEEEEEEECCCCHHHHHCCCCCCCCC
PRDIARMVLFLGSDDAAMCTAQEFKVDAGWV
HHHHHHHHHEECCCCHHEEEHHHHCCCCCCC
>Mature Secondary Structure 
SQISPVPSSGAAASPSGALSVFPSLRGRVVFVTGGGSGIGAAIVTAFAEQGARVAFVDV
CCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEH
AREASGQLVRDMEAAGLPRPWWRECDVRDIDALRAALRDAAAELGDVSVLVNNVASDDRH
HHHHCCHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHH
TLESVTPEYYDERMAINERPAFFAIQAVVPGMRRLGGGSIVNLGSTGWQGKGSGYPCYAI
HHHHCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEE
AKSSVNGLTRGLARTLGQDRIRINTVSPGWVMTERQIRLWLDAEGERELARNQCLPDKLQ
HHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCEEEEEEECCCCHHHHHCCCCCCCCC
PRDIARMVLFLGSDDAAMCTAQEFKVDAGWV
HHHHHHHHHEECCCCHHEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9655913; 12597275 [H]