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The map label for this gene is dmlA [H]

Identifier: 120610865

GI number: 120610865

Start: 2410422

End: 2411513

Strand: Direct

Name: dmlA [H]

Synonym: Aave_2191

Alternate gene names: 120610865

Gene position: 2410422-2411513 (Clockwise)

Preceding gene: 120610864

Following gene: 120610866

Centisome position: 45.03

GC content: 69.14

Gene sequence:

>1092_bases
ATGGAAACCATTTTCCGCATCGCGGTGCTGCCGGGAGACGGCATCGGCAAGGAAGTCATGCCCGAGGGGCTGCGGGCGAT
CCAGGCGGCGGCCGAGCGCTTCGGCTTCGCGCTGGAGCTGCACACCTTCGAGTGGGCCAGCTGCGACTACTACCTCGCGC
ACGGGCAGATGATGCCCGACGACTGGAAGGAGCAGCTCAAGGGCATGGACGCGCTCTTCTTCGGCGCCGTGGGCTGGCCG
GCCACCGTGCCCGACCACGTCTCGCTGTGGGGCTCGCTGCTGAAGTTCCGCCGCGAGTTCGACCAGTACATCAACCTGCG
GCCCGTGCGCCTGTTCGAGGGCGTGCCGTGCCCGCTGGCGGGCCGCAAGCCCGGGGACATCGACTACTACGTGGTGCGCG
AGAACACCGAGGGCGAGTACACGTCGCTGGGCGGCATCATGTACGAGGGCACGGACCGCGAGATCGTGATCCAGGAGTCG
GTGTATTCGCGCAAGGGCGCCGAGCGGCTGCTGAAGTTCGCGTTCGATCTCGCGCAGAGCCGCCCGAAGAAGCACGTGAC
GCTGGCCACCAAGAGCAACGGCATCGCCATCAGCATGCCGTGGTGGGACCGGCGGGCCGACGAGGTGGCCAGACAGTATC
CCGGCATCACGCTGGACAAGCAGCACATCGACATCCTGACGGCGCGCTTCGTGCTGCAGCCGGGGCGCTTCGACGTGGTG
GCGGCGACGAACCTGTTCGGCGACATCCTGTCGGACCTGGGGCCGGCGACGACGGGCACGATCGGGCTGGCGCCGTCGGC
GAACCTGAACCCGGAGCGCACGTTCCCGAGCCTGTTCGAGCCGGTGCACGGCTCGGCGCCGGACATCTACGGCAGGAACA
TCGCCAACCCGATCGCGATGGTGTGGTCGGGGGCGCTGATGCTGGACTTCCTGACGCAGGGCCAGGGGGCGGGGCGGCAG
GCGCACGATGCGATCGTGGCGGCCATCGAGCAGGTGATCCAGAGCGGGCCGAAGACGCCGGACCTGGGCGGCACGGCCAG
CACCACGCAGGTGGGCGAAGCCATCGCGGCGGCCATCGCCGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCCGAACTGCGCCAGGGCTGGCTGGAGGAGCAGGACAGGGCGGTCCCGGGCCGCACCCATGCGGCTCAGGCTGCATAAC
CCAACGAACCGAGGAAGAAC

Downstream 100 bases:

>100_bases
GCCCGCCATGGGACCGACCTTCCTCCCGCCGACGAACTGTTGACCCCGCCGTGCAACGGCTGCCTTTTTCCGCACCATGA
CCTCTTCCGCTTCCACTCCC

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
METIFRIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPDDWKEQLKGMDALFFGAVGWP
ATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQES
VYSRKGAERLLKFAFDLAQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRFDVV
AATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAMVWSGALMLDFLTQGQGAGRQ
AHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIAAAA

Sequences:

>Translated_363_residues
METIFRIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPDDWKEQLKGMDALFFGAVGWP
ATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQES
VYSRKGAERLLKFAFDLAQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRFDVV
AATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAMVWSGALMLDFLTQGQGAGRQ
AHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIAAAA
>Mature_363_residues
METIFRIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPDDWKEQLKGMDALFFGAVGWP
ATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQES
VYSRKGAERLLKFAFDLAQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRFDVV
AATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAMVWSGALMLDFLTQGQGAGRQ
AHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIAAAA

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=357, Percent_Identity=31.0924369747899, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI28178816, Length=368, Percent_Identity=27.7173913043478, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178821, Length=358, Percent_Identity=27.6536312849162, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=28.1976744186047, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=343, Percent_Identity=26.8221574344023, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI28178819, Length=207, Percent_Identity=28.9855072463768, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=72.1448467966574, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI87081683, Length=371, Percent_Identity=38.0053908355795, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1787381, Length=386, Percent_Identity=28.2383419689119, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17550882, Length=353, Percent_Identity=28.328611898017, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71986051, Length=361, Percent_Identity=30.7479224376731, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=357, Percent_Identity=26.3305322128852, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=27.0422535211268, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=36.2116991643454, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=32.6027397260274, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=360, Percent_Identity=31.6666666666667, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=31.4606741573034, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643270, Length=360, Percent_Identity=31.6666666666667, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=31.6666666666667, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=29.6918767507003, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI161078635, Length=371, Percent_Identity=27.7628032345013, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI161078637, Length=371, Percent_Identity=27.7628032345013, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI161078633, Length=371, Percent_Identity=27.7628032345013, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24650122, Length=371, Percent_Identity=27.7628032345013, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078639, Length=369, Percent_Identity=27.9132791327913, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=28.2913165266106, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=28.2913165266106, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20130355, Length=356, Percent_Identity=22.752808988764, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39610; Mature: 39610

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIFRIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPD
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCEEEECCCCCCH
DWKEQLKGMDALFFGAVGWPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLA
HHHHHHCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCC
GRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQESVYSRKGAERLLKFAFDLAQS
CCCCCCCEEEEEEECCCCCEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
RPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRFDVV
CCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHEECCCCEEEE
AATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAM
EHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHH
VWSGALMLDFLTQGQGAGRQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
AAA
HCC
>Mature Secondary Structure
METIFRIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPD
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCEEEECCCCCCH
DWKEQLKGMDALFFGAVGWPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLA
HHHHHHCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCC
GRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQESVYSRKGAERLLKFAFDLAQS
CCCCCCCEEEEEEECCCCCEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
RPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRFDVV
CCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHEECCCCEEEE
AATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAM
EHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHH
VWSGALMLDFLTQGQGAGRQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
AAA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]