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The map label for this gene is paaF [C]

Identifier: 120610839

GI number: 120610839

Start: 2379329

End: 2380171

Strand: Direct

Name: paaF [C]

Synonym: Aave_2165

Alternate gene names: 120610839

Gene position: 2379329-2380171 (Clockwise)

Preceding gene: 120610838

Following gene: 120610840

Centisome position: 44.45

GC content: 71.53

Gene sequence:

>843_bases
ATGACCGAACCCGCCGCTTCCACGTCCGCCTCCGCCGTCGGCGCCCCGTTGCTCCACACCACGCGCGATGCGCGGGGCGT
CGTGACCCTCACGCTGGCCGATCCGGCGCGGCTGAATGCGCTCGGCGACGCCATGCTCGCCGCGCTGCAGCAGGCGCTCG
ATGCGGTCGCGCAGGACCCGCAGGCCCGCGTGGTGGTGCTGGCCGCGCAGGGCAAGGCGTTCTGCGCGGGCCATGACCTG
AAGGACATGGCCGCGCACCCGGATGCCGCCTGGTACAGGCGCCTCTTCGCGCAGTGCAGCCGCATGATGGTGTCGATCCA
GCGCCTGCCCGTGCCGGTGATCGCGCGCGTGCAGGGCATCGCCACCGCGGCGGGCTGCCAGCTGGTCGCGCAGTGCGATC
TGGCCGTGGCGGCCGAAGGAGCGCGCTTCGCCACCAGCGGCATCCACTACGGCCTTTTCTGCTCCACGCCCAGCGTGCCG
CTGGTGCGCAACGTGCCGGCGAAACGCGCCATGGAGATGCTGCTCACCGGCGACTTCATCGACGCGCGCACCGCACTGGC
CGAAGGCCTGGTGAACCGCGTCGTGCCGGCCGAGGCACTGGACGACGCGGTGGACTCGCTCGTGTGCTCGATTCTCGGCA
AGCCCCGCGCCGCGGTGGCGATGGGCAAGGCGCTCTTCTACCAGCAGCGTGAACTTGGCGTGGAAGCGGCCTACCAGCTC
GCCGGGCAGGCCATGGCCACGAACATGATGGACGAGGCGGCGCAGGAAGGTGCGCGCGCGTTCGCCGAGAAACGGGAGCC
GGCCTGGAAAGCCACGAGCGAGGCGTCGCACAGGCCTGAGTGA

Upstream 100 bases:

>100_bases
GTGATGGATGCCCTCGCGCGCAGCGTGGAAGCCCACGTGCGCGTGCTGGCCGACCTCTGAGGCGAACCGCCCGCCATACA
GCCCAAGCTTCTTCGCCGCC

Downstream 100 bases:

>100_bases
GGTTGCGCTTCACTATCGATAAGCGGCTCCAGCATTCCTCCTGCTGCTGCGGGATGCATTCATGACGTGTCGGATGCGGC
CAGTGTGTCCCATGCGTAGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTEPAASTSASAVGAPLLHTTRDARGVVTLTLADPARLNALGDAMLAALQQALDAVAQDPQARVVVLAAQGKAFCAGHDL
KDMAAHPDAAWYRRLFAQCSRMMVSIQRLPVPVIARVQGIATAAGCQLVAQCDLAVAAEGARFATSGIHYGLFCSTPSVP
LVRNVPAKRAMEMLLTGDFIDARTALAEGLVNRVVPAEALDDAVDSLVCSILGKPRAAVAMGKALFYQQRELGVEAAYQL
AGQAMATNMMDEAAQEGARAFAEKREPAWKATSEASHRPE

Sequences:

>Translated_280_residues
MTEPAASTSASAVGAPLLHTTRDARGVVTLTLADPARLNALGDAMLAALQQALDAVAQDPQARVVVLAAQGKAFCAGHDL
KDMAAHPDAAWYRRLFAQCSRMMVSIQRLPVPVIARVQGIATAAGCQLVAQCDLAVAAEGARFATSGIHYGLFCSTPSVP
LVRNVPAKRAMEMLLTGDFIDARTALAEGLVNRVVPAEALDDAVDSLVCSILGKPRAAVAMGKALFYQQRELGVEAAYQL
AGQAMATNMMDEAAQEGARAFAEKREPAWKATSEASHRPE
>Mature_279_residues
TEPAASTSASAVGAPLLHTTRDARGVVTLTLADPARLNALGDAMLAALQQALDAVAQDPQARVVVLAAQGKAFCAGHDLK
DMAAHPDAAWYRRLFAQCSRMMVSIQRLPVPVIARVQGIATAAGCQLVAQCDLAVAAEGARFATSGIHYGLFCSTPSVPL
VRNVPAKRAMEMLLTGDFIDARTALAEGLVNRVVPAEALDDAVDSLVCSILGKPRAAVAMGKALFYQQRELGVEAAYQLA
GQAMATNMMDEAAQEGARAFAEKREPAWKATSEASHRPE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=42.3076923076923, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI194097323, Length=264, Percent_Identity=32.1969696969697, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI70995211, Length=262, Percent_Identity=26.7175572519084, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=26.0536398467433, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=32.6771653543307, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=32.824427480916, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082183, Length=244, Percent_Identity=30.327868852459, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=30.1158301158301, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=30.8880308880309, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=29.0502793296089, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=248, Percent_Identity=40.3225806451613, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25145438, Length=264, Percent_Identity=32.9545454545455, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=27.6, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=30.0546448087432, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=193, Percent_Identity=29.5336787564767, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=249, Percent_Identity=24.0963855421687, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=284, Percent_Identity=22.887323943662, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=38.5542168674699, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=30.6201550387597, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=30.6201550387597, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=26.953125, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29437; Mature: 29306

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPAASTSASAVGAPLLHTTRDARGVVTLTLADPARLNALGDAMLAALQQALDAVAQDP
CCCCCCCCCCHHHCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
QARVVVLAAQGKAFCAGHDLKDMAAHPDAAWYRRLFAQCSRMMVSIQRLPVPVIARVQGI
CCEEEEEEECCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ATAAGCQLVAQCDLAVAAEGARFATSGIHYGLFCSTPSVPLVRNVPAKRAMEMLLTGDFI
HHHHHHHHHHHCCHHHHCCCCHHHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHCCCHH
DARTALAEGLVNRVVPAEALDDAVDSLVCSILGKPRAAVAMGKALFYQQRELGVEAAYQL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGQAMATNMMDEAAQEGARAFAEKREPAWKATSEASHRPE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEPAASTSASAVGAPLLHTTRDARGVVTLTLADPARLNALGDAMLAALQQALDAVAQDP
CCCCCCCCCHHHCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
QARVVVLAAQGKAFCAGHDLKDMAAHPDAAWYRRLFAQCSRMMVSIQRLPVPVIARVQGI
CCEEEEEEECCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ATAAGCQLVAQCDLAVAAEGARFATSGIHYGLFCSTPSVPLVRNVPAKRAMEMLLTGDFI
HHHHHHHHHHHCCHHHHCCCCHHHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHCCCHH
DARTALAEGLVNRVVPAEALDDAVDSLVCSILGKPRAAVAMGKALFYQQRELGVEAAYQL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGQAMATNMMDEAAQEGARAFAEKREPAWKATSEASHRPE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA