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The map label for this gene is xthA [H]

Identifier: 120610799

GI number: 120610799

Start: 2333704

End: 2334492

Strand: Direct

Name: xthA [H]

Synonym: Aave_2122

Alternate gene names: 120610799

Gene position: 2333704-2334492 (Clockwise)

Preceding gene: 120610798

Following gene: 120610803

Centisome position: 43.6

GC content: 72.24

Gene sequence:

>789_bases
ATGAAAATCGCCACCTTCAACGTCAACAGCATCCGCGCCCGCCTGCCGGCGCTGCTCCAGTGGCTGGAGGAATCCCGCCC
CGATGTCGCCTGCCTGCAGGAACTCAAGTGCACCGACGACGCCTTCCCGGCCGGGGAGATCCGGGCCGCGGGCTACGGCG
CGCTCTGGCACGGGCAGAAGGCCTGGAACGGCGTGGCCATCCTCGCGCGCGGTGCCAATCCCATCGAGGTGCAGCGCGGG
CTGCCCGGCGACGAAGCCGACAGCCAGAGCCGCTACCTGGAGGCCGCCGTGGACGGCGTGCTGGTCGCCTGCCTCTACCT
GCCCAACGGCAACCCGGCCCCCGGCCCGAAGTTCGACTACAAGCTCGCGTGGCTCGACCGCCTCGCGCGGCGGGCCGGCG
TGCTGCGCGACCGCCCCGAGCCGGTGGTGCTGGCGGGGGACTTCAACATCATTCCCACCGAGCGCGACGTGTACAAACCC
GGGGCCTGGGTGGACGACGCGTTGTTCCGCTCCGAGGTGCGCGATGCCTTCCGCGCCCTGGTGGACAGCGGCTGGACCGA
CGCGCTTCGCGCGGTGCATCCCGACGAGACGGTCTATACCTTCTGGGACTACCTGCGCCAGCGCTTTCAGCGCAACGCCG
GCCTGCGTATCGACCACCTGCTGCTCAACCCCGCGGCGGCCCACCGCCTGCAGGACGCGGGCGTGGACCGCGAAGTGCGC
GGCCGGCCCCGGCCCAGCGACCATGCCCCGGCCTGGGCGCTGCTGGCACCGGGGCAGGGCGGGACCTGA

Upstream 100 bases:

>100_bases
GGCCGCCTGAGCTTTGCGGCCCGGCTGGCGGCACGACGGCGCCGCCGCCTACCACCGGCCCGCCGCCGCGGCGTAGCCTT
GGGGGCTCGAAAGAGCCGCC

Downstream 100 bases:

>100_bases
GCCGCCGCCCACCAGGCGTCAGGCGCCAGGCTCTCCAGCCAGCGCCCGGGCGAGGTAGGGCGCGGTCCGGCTGCCGGCCT
GGCGCGCGACCTCGGACGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKIATFNVNSIRARLPALLQWLEESRPDVACLQELKCTDDAFPAGEIRAAGYGALWHGQKAWNGVAILARGANPIEVQRG
LPGDEADSQSRYLEAAVDGVLVACLYLPNGNPAPGPKFDYKLAWLDRLARRAGVLRDRPEPVVLAGDFNIIPTERDVYKP
GAWVDDALFRSEVRDAFRALVDSGWTDALRAVHPDETVYTFWDYLRQRFQRNAGLRIDHLLLNPAAAHRLQDAGVDREVR
GRPRPSDHAPAWALLAPGQGGT

Sequences:

>Translated_262_residues
MKIATFNVNSIRARLPALLQWLEESRPDVACLQELKCTDDAFPAGEIRAAGYGALWHGQKAWNGVAILARGANPIEVQRG
LPGDEADSQSRYLEAAVDGVLVACLYLPNGNPAPGPKFDYKLAWLDRLARRAGVLRDRPEPVVLAGDFNIIPTERDVYKP
GAWVDDALFRSEVRDAFRALVDSGWTDALRAVHPDETVYTFWDYLRQRFQRNAGLRIDHLLLNPAAAHRLQDAGVDREVR
GRPRPSDHAPAWALLAPGQGGT
>Mature_262_residues
MKIATFNVNSIRARLPALLQWLEESRPDVACLQELKCTDDAFPAGEIRAAGYGALWHGQKAWNGVAILARGANPIEVQRG
LPGDEADSQSRYLEAAVDGVLVACLYLPNGNPAPGPKFDYKLAWLDRLARRAGVLRDRPEPVVLAGDFNIIPTERDVYKP
GAWVDDALFRSEVRDAFRALVDSGWTDALRAVHPDETVYTFWDYLRQRFQRNAGLRIDHLLLNPAAAHRLQDAGVDREVR
GRPRPSDHAPAWALLAPGQGGT

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=30.0380228136882, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=30.0380228136882, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=30.0380228136882, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=35.9550561797753, Blast_Score=151, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=26.953125, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221330655, Length=256, Percent_Identity=31.640625, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI17136678, Length=256, Percent_Identity=31.640625, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29007; Mature: 29007

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATFNVNSIRARLPALLQWLEESRPDVACLQELKCTDDAFPAGEIRAAGYGALWHGQK
CEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEECCCCCEECCCC
AWNGVAILARGANPIEVQRGLPGDEADSQSRYLEAAVDGVLVACLYLPNGNPAPGPKFDY
CCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
KLAWLDRLARRAGVLRDRPEPVVLAGDFNIIPTERDVYKPGAWVDDALFRSEVRDAFRAL
HHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VDSGWTDALRAVHPDETVYTFWDYLRQRFQRNAGLRIDHLLLNPAAAHRLQDAGVDREVR
HHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEHEEECHHHHHHHHHCCCCCHHC
GRPRPSDHAPAWALLAPGQGGT
CCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKIATFNVNSIRARLPALLQWLEESRPDVACLQELKCTDDAFPAGEIRAAGYGALWHGQK
CEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEECCCCCEECCCC
AWNGVAILARGANPIEVQRGLPGDEADSQSRYLEAAVDGVLVACLYLPNGNPAPGPKFDY
CCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
KLAWLDRLARRAGVLRDRPEPVVLAGDFNIIPTERDVYKPGAWVDDALFRSEVRDAFRAL
HHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VDSGWTDALRAVHPDETVYTFWDYLRQRFQRNAGLRIDHLLLNPAAAHRLQDAGVDREVR
HHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEHEEECHHHHHHHHHCCCCCHHC
GRPRPSDHAPAWALLAPGQGGT
CCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]