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The map label for this gene is pmbA [H]

Identifier: 120610757

GI number: 120610757

Start: 2280323

End: 2281780

Strand: Direct

Name: pmbA [H]

Synonym: Aave_2079

Alternate gene names: 120610757

Gene position: 2280323-2281780 (Clockwise)

Preceding gene: 120610754

Following gene: 120610762

Centisome position: 42.6

GC content: 71.06

Gene sequence:

>1458_bases
ATGAAGACCACCCGCCCCCGTACCGCAGCCACCGCCGCTGCCACCTCCTCCGCCCGCTCCTGCGCTTCCGCGTCCGCCCC
TGCCGGCGGCGATGCCGGCAACGGCTTCAGCTACAGCCGCGGCGTGTTCGAGGAACTGGTGGACCGGGCCCTGGCCCACG
CGCTGCGCCTGGGCGCCACCGATGCGGGCGCCGAGGCATCCGAGGGCTTCGGCCTGTCGGTGAGCGTGCGCAAGGGCGAA
CTGGAGACGGTGGAGCGCAACCGCGACAAGTCGCTGGGCATCACCGTGTACGTGGGCAACCGCCGGGGCAACGCCAGCAC
GTCCGACTTCTCCGAGCGCGCCATCGAGCAGACCGTGCAGGCCGCCTACGACATCGCCCGCTTCACCGCCGAAGACCCGG
TGGCCGGCCTGCCCGACGTGGCGGACGTGGCCGCGCCCGGCACGCACCGCGAGCTGGACCTGTTCCATCCCTGGGCCATC
ACCAGCGAAGAGGCCGCCCGCATCGCGCTCGAGTGCGAGGACGCCGCCCTGCGCACGCACCGCCGCATCACCAACAGCGA
AGGCGCCGCCGTCTCGGCCCAGCAGAGCCACTTCTTCAGCGCGCACACCCACGGCTTCCGCGGGGGGTACGCCAGCTCGC
GCCACAGCATCTCGGTGGCGCCCATCGCCTCGCTGCCGGGCCGCAATGCCGAGATGCAGCGCGACGCGTGGTATTCGTCC
ATGCGCGACGCGGCCGAGCTGTCCTCGCCCGCCGCCGTGGGCCGCTACGCGGCCGAGCGCGCCCTGAGCCGCCTGGGCAG
CCGCAAGATCCCCACCACCGAATGCCCGGTGCTGTTCGAATCACCGCTGGCCGCGGGCCTGCTCGGCGGCTTCACCCAGG
CCGTGAGCGGCGGGGCGCTCTACCGCAAGAGCAGCTTCCTGCTCGACTCGCTGGGCCGCCGGGTCTTCCCGGACCACATC
GACATCGACGAGGACCCGTTCGTCGCGCGCGGCAAGGGCAGCTCGCCCTTCGACGAGGAGGGCGTGCGCGTGGCCCCGCG
CAAGGTGGTGGATGCCGGCGAGGTGCAGGGCTACTTCCTCAGCAGCTACTCCGCCCGCAAGCTCGGCATGAAGACCACCG
GCAACGCCGGGGGCTCGCACAACCTCGCGCTCACCTCGCGCCTCACGCAGCCAGGCGACGACCTGGACGCCATGCTGAAG
AAGCTCGGCACGGGCCTGTTCGTGATCGAGCTCATGGGTCAGGGCGTGAACTACGTGACCGGCGACTATTCCCGCGGCGC
CAGCGGTTTCTGGGTGGAGAACGGCGAGATCGCCTTCCCCGTGCACGAGATCACCATCGCGGGCAACCTCAAGGACATGT
TCCTCGGCATCGAGGCCGTGGGCGCGGACGCCTACAACTACGGTGCCAAGACCGTGGGTTCGGTGCTGGTCAATCGCATG
AAGGTGGCGGGCAGCTGA

Upstream 100 bases:

>100_bases
CTTCACGAAATAGCCTTTTTTGGGTTTGCGGGACATGGGAGAGGGGACAAAAAGAGGCCCTGGGCGGGGGCGGAAGGCGG
CAAGTATCATAGCCGGCGAT

Downstream 100 bases:

>100_bases
CACCGGGGCGCCGCAGGGTCGGCGCGAGATGGATGGCGGAAACGAGGAATTCCGCGTGATCAGCGTTGGTACTCCGGTGA
CGGCTGTATCCCGACCATGG

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKTTRPRTAATAAATSSARSCASASAPAGGDAGNGFSYSRGVFEELVDRALAHALRLGATDAGAEASEGFGLSVSVRKGE
LETVERNRDKSLGITVYVGNRRGNASTSDFSERAIEQTVQAAYDIARFTAEDPVAGLPDVADVAAPGTHRELDLFHPWAI
TSEEAARIALECEDAALRTHRRITNSEGAAVSAQQSHFFSAHTHGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSS
MRDAAELSSPAAVGRYAAERALSRLGSRKIPTTECPVLFESPLAAGLLGGFTQAVSGGALYRKSSFLLDSLGRRVFPDHI
DIDEDPFVARGKGSSPFDEEGVRVAPRKVVDAGEVQGYFLSSYSARKLGMKTTGNAGGSHNLALTSRLTQPGDDLDAMLK
KLGTGLFVIELMGQGVNYVTGDYSRGASGFWVENGEIAFPVHEITIAGNLKDMFLGIEAVGADAYNYGAKTVGSVLVNRM
KVAGS

Sequences:

>Translated_485_residues
MKTTRPRTAATAAATSSARSCASASAPAGGDAGNGFSYSRGVFEELVDRALAHALRLGATDAGAEASEGFGLSVSVRKGE
LETVERNRDKSLGITVYVGNRRGNASTSDFSERAIEQTVQAAYDIARFTAEDPVAGLPDVADVAAPGTHRELDLFHPWAI
TSEEAARIALECEDAALRTHRRITNSEGAAVSAQQSHFFSAHTHGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSS
MRDAAELSSPAAVGRYAAERALSRLGSRKIPTTECPVLFESPLAAGLLGGFTQAVSGGALYRKSSFLLDSLGRRVFPDHI
DIDEDPFVARGKGSSPFDEEGVRVAPRKVVDAGEVQGYFLSSYSARKLGMKTTGNAGGSHNLALTSRLTQPGDDLDAMLK
KLGTGLFVIELMGQGVNYVTGDYSRGASGFWVENGEIAFPVHEITIAGNLKDMFLGIEAVGADAYNYGAKTVGSVLVNRM
KVAGS
>Mature_485_residues
MKTTRPRTAATAAATSSARSCASASAPAGGDAGNGFSYSRGVFEELVDRALAHALRLGATDAGAEASEGFGLSVSVRKGE
LETVERNRDKSLGITVYVGNRRGNASTSDFSERAIEQTVQAAYDIARFTAEDPVAGLPDVADVAAPGTHRELDLFHPWAI
TSEEAARIALECEDAALRTHRRITNSEGAAVSAQQSHFFSAHTHGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSS
MRDAAELSSPAAVGRYAAERALSRLGSRKIPTTECPVLFESPLAAGLLGGFTQAVSGGALYRKSSFLLDSLGRRVFPDHI
DIDEDPFVARGKGSSPFDEEGVRVAPRKVVDAGEVQGYFLSSYSARKLGMKTTGNAGGSHNLALTSRLTQPGDDLDAMLK
KLGTGLFVIELMGQGVNYVTGDYSRGASGFWVENGEIAFPVHEITIAGNLKDMFLGIEAVGADAYNYGAKTVGSVLVNRM
KVAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=449, Percent_Identity=48.9977728285078, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 51209; Mature: 51209

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTRPRTAATAAATSSARSCASASAPAGGDAGNGFSYSRGVFEELVDRALAHALRLGAT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DAGAEASEGFGLSVSVRKGELETVERNRDKSLGITVYVGNRRGNASTSDFSERAIEQTVQ
CCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
AAYDIARFTAEDPVAGLPDVADVAAPGTHRELDLFHPWAITSEEAARIALECEDAALRTH
HHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCHHHEEEEEECCHHHHHHH
RRITNSEGAAVSAQQSHFFSAHTHGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSS
HHHCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHH
MRDAAELSSPAAVGRYAAERALSRLGSRKIPTTECPVLFESPLAAGLLGGFTQAVSGGAL
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCHH
YRKSSFLLDSLGRRVFPDHIDIDEDPFVARGKGSSPFDEEGVRVAPRKVVDAGEVQGYFL
HHHHHHHHHHHCHHCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCHHHCCCCCCCEEEE
SSYSARKLGMKTTGNAGGSHNLALTSRLTQPGDDLDAMLKKLGTGLFVIELMGQGVNYVT
CCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEHHCCCCCEEE
GDYSRGASGFWVENGEIAFPVHEITIAGNLKDMFLGIEAVGADAYNYGAKTVGSVLVNRM
CCCCCCCCCEEEECCEEEEEEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KVAGS
HHCCC
>Mature Secondary Structure
MKTTRPRTAATAAATSSARSCASASAPAGGDAGNGFSYSRGVFEELVDRALAHALRLGAT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DAGAEASEGFGLSVSVRKGELETVERNRDKSLGITVYVGNRRGNASTSDFSERAIEQTVQ
CCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
AAYDIARFTAEDPVAGLPDVADVAAPGTHRELDLFHPWAITSEEAARIALECEDAALRTH
HHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCHHHEEEEEECCHHHHHHH
RRITNSEGAAVSAQQSHFFSAHTHGFRGGYASSRHSISVAPIASLPGRNAEMQRDAWYSS
HHHCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHH
MRDAAELSSPAAVGRYAAERALSRLGSRKIPTTECPVLFESPLAAGLLGGFTQAVSGGAL
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCHH
YRKSSFLLDSLGRRVFPDHIDIDEDPFVARGKGSSPFDEEGVRVAPRKVVDAGEVQGYFL
HHHHHHHHHHHCHHCCCCCCCCCCCCCEECCCCCCCCCCCCCEECCHHHCCCCCCCEEEE
SSYSARKLGMKTTGNAGGSHNLALTSRLTQPGDDLDAMLKKLGTGLFVIELMGQGVNYVT
CCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEHHCCCCCEEE
GDYSRGASGFWVENGEIAFPVHEITIAGNLKDMFLGIEAVGADAYNYGAKTVGSVLVNRM
CCCCCCCCCEEEECCEEEEEEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KVAGS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]