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The map label for this gene is glnA [H]

Identifier: 120610129

GI number: 120610129

Start: 1588828

End: 1590243

Strand: Direct

Name: glnA [H]

Synonym: Aave_1442

Alternate gene names: 120610129

Gene position: 1588828-1590243 (Clockwise)

Preceding gene: 120610128

Following gene: 120610130

Centisome position: 29.68

GC content: 63.63

Gene sequence:

>1416_bases
ATGGCAAAGACCGTTGCAGACGTGATGAAGATGGTGAAGGAGAACGAAGTCAAGTTTGTGGACTTCCGCTTCACCGACAC
CCGCGGCAAGGAGCAGCACGTCACCGTGCCGGTTTCCCATTTCGACGAAGACAAGTTCTCGTCGGGCCACGCTTTCGACG
GTTCTTCGGTGGCGGGCTGGAAGGGCATCGAGGCTTCGGACATGCAGCTCATGCCCGACCCCAACACCGCCAACATCGAT
CCGTTCTTCGAGGAAACCACGCTGTTCCTGCAGTGCGACGTGATCGAGCCGGGCGACGGCAAGGCCTATGACCGCGATCC
CCGCTCCATCGCCAAGCGCGCGGAAGCCTACCTGAAGGCCTCGGGCCTGGGCGACACGGCTTTCTTCGGTCCGGAACCCG
AGTTCTTCATTTTCGATGGCGTGCGCTGGAGCAACGAGCCCGGCAAGGTCATGTTCGAGATCGAAGAGTACGAAGCGCCC
TGGAACTCCGGCGCGAAGCTGGAAGGCGGCAACCGCGGCCACCGTCCCACGGTCAAGGGCGGCTACTTCCCCGTGCCTCC
CGTGGACAGCACGCAGGACATGCGCGCCGAAATGGCCCTGATCCTCGAATCGCTGGGCATCCCGGTCGAGGTGTTCCACC
ATGAAGTGGCCGGTGCCGGCCAGAACGAAATCGGCACCAAGTTCAGCACGCTGGTCGAGCGTGCCGACTGGACCCAGGTG
CTCAAGTACGTGGTGTGGAACGTGGCCAACGCCTACGGCAAGACCGCCACCTTCATGCCCAAGCCCTACGCCGGCGACAA
CGGCTCCGGCATGCACGTGCACCAGTCCATCTGGAAGGACGGCAAGAACCTGTTCGCCGGCGACGGCTATGCGGGCCTCT
CCGACTTCGCGCTGTACTACATCGGCGGCATCATCAAGCACGCCCGTGCCCTGAATGCCATCACCAATCCCGGCACGAAC
AGCTACAAGCGCCTGGTGCCCGGTTTCGAGGCTCCCGTGAAGCTGGCCTACTCCGCCCGCAACCGCTCGGCCTCGATCCG
CATCCCGTACGTGGCCAACCCCAAGGGCCGCCGCATCGAGGCGCGTTTCCCGGATCCTTCGGCGAACCCGTACCTGTGCT
TCTCCGCACTGATGATGGCCGGCCTGGACGGCGTGGAAAACAAGATCCATCCGGGCGAAGCCGCCACGAAGGACCTCTAC
CACCTGCCGCCCGAGGAAGACAAGCTGGTGCCCACCGTGTGCCACAGCCTCGACCAGGCGCTGGAGTACCTGGACAAGGA
CCGCGGCTTCCTGACCAAGGGCGGCGTGTTCTCGGACTCCATGCTGGACGCCTACATCGAGCTGAAGATGGGCGAAGTCA
CGCGCTTCCGCATGGCTCCGCACCCGGTCGAGTACGACATGTACTACTCGCTGTGA

Upstream 100 bases:

>100_bases
TGCGGAAGACGTGCATGCTCCGCGTTAGTGCATGCTGGCACGTTATCTGCTTTTTGTAGGCCGTGTTTTTTTCAACAAAC
GCTAAATCCTGGAGATCCTG

Downstream 100 bases:

>100_bases
TCAGCGCGGCCCGGGCCTCGTGCCTGGGGGCGAGATGCGACAGGGCGGCTTCGGCCGCCCTTTTTCATGGGCGCGGACTC
CATGCGCGGTTCCAGGGGCC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFSSGHAFDGSSVAGWKGIEASDMQLMPDPNTANID
PFFEETTLFLQCDVIEPGDGKAYDRDPRSIAKRAEAYLKASGLGDTAFFGPEPEFFIFDGVRWSNEPGKVMFEIEEYEAP
WNSGAKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTKFSTLVERADWTQV
LKYVVWNVANAYGKTATFMPKPYAGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTN
SYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIEARFPDPSANPYLCFSALMMAGLDGVENKIHPGEAATKDLY
HLPPEEDKLVPTVCHSLDQALEYLDKDRGFLTKGGVFSDSMLDAYIELKMGEVTRFRMAPHPVEYDMYYSL

Sequences:

>Translated_471_residues
MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFSSGHAFDGSSVAGWKGIEASDMQLMPDPNTANID
PFFEETTLFLQCDVIEPGDGKAYDRDPRSIAKRAEAYLKASGLGDTAFFGPEPEFFIFDGVRWSNEPGKVMFEIEEYEAP
WNSGAKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTKFSTLVERADWTQV
LKYVVWNVANAYGKTATFMPKPYAGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTN
SYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIEARFPDPSANPYLCFSALMMAGLDGVENKIHPGEAATKDLY
HLPPEEDKLVPTVCHSLDQALEYLDKDRGFLTKGGVFSDSMLDAYIELKMGEVTRFRMAPHPVEYDMYYSL
>Mature_470_residues
AKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFSSGHAFDGSSVAGWKGIEASDMQLMPDPNTANIDP
FFEETTLFLQCDVIEPGDGKAYDRDPRSIAKRAEAYLKASGLGDTAFFGPEPEFFIFDGVRWSNEPGKVMFEIEEYEAPW
NSGAKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTKFSTLVERADWTQVL
KYVVWNVANAYGKTATFMPKPYAGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTNS
YKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIEARFPDPSANPYLCFSALMMAGLDGVENKIHPGEAATKDLYH
LPPEEDKLVPTVCHSLDQALEYLDKDRGFLTKGGVFSDSMLDAYIELKMGEVTRFRMAPHPVEYDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=64.4396551724138, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=391, Percent_Identity=26.8542199488491, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52250; Mature: 52119

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFSSGHAFDGSSVAGW
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMQLMPDPNTANIDPFFEETTLFLQCDVIEPGDGKAYDRDPRSIAKRAEAYLKA
CCCCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
SGLGDTAFFGPEPEFFIFDGVRWSNEPGKVMFEIEEYEAPWNSGAKLEGGNRGHRPTVKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCC
GYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTKFSTLVERADWTQV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHH
LKYVVWNVANAYGKTATFMPKPYAGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEE
ARFPDPSANPYLCFSALMMAGLDGVENKIHPGEAATKDLYHLPPEEDKLVPTVCHSLDQA
EECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
LEYLDKDRGFLTKGGVFSDSMLDAYIELKMGEVTRFRMAPHPVEYDMYYSL
HHHHHCCCCEEECCCCCCHHHHHHHHEEEECCEEEEECCCCCCCCCEEECC
>Mature Secondary Structure 
AKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDEDKFSSGHAFDGSSVAGW
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMQLMPDPNTANIDPFFEETTLFLQCDVIEPGDGKAYDRDPRSIAKRAEAYLKA
CCCCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
SGLGDTAFFGPEPEFFIFDGVRWSNEPGKVMFEIEEYEAPWNSGAKLEGGNRGHRPTVKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCC
GYFPVPPVDSTQDMRAEMALILESLGIPVEVFHHEVAGAGQNEIGTKFSTLVERADWTQV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHH
LKYVVWNVANAYGKTATFMPKPYAGDNGSGMHVHQSIWKDGKNLFAGDGYAGLSDFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEE
ARFPDPSANPYLCFSALMMAGLDGVENKIHPGEAATKDLYHLPPEEDKLVPTVCHSLDQA
EECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
LEYLDKDRGFLTKGGVFSDSMLDAYIELKMGEVTRFRMAPHPVEYDMYYSL
HHHHHCCCCEEECCCCCCHHHHHHHHEEEECCEEEEECCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]