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The map label for this gene is eno [H]

Identifier: 120610009

GI number: 120610009

Start: 1448521

End: 1449807

Strand: Reverse

Name: eno [H]

Synonym: Aave_1322

Alternate gene names: 120610009

Gene position: 1449807-1448521 (Counterclockwise)

Preceding gene: 120610010

Following gene: 120610008

Centisome position: 27.09

GC content: 66.67

Gene sequence:

>1287_bases
ATGAGTGCCATCGTTGATATCGTCGGCCGCGAAGTGCTGGACAGCCGCGGCAACCCCACCGTCGAATGCGACGTGCTGCT
GGAGTCGGGCGTGATGGGCCGCGCGGCCGTGCCCTCCGGCGCCTCCACCGGCAGCCGCGAAGCCATCGAACTGCGCGACG
GCGACAAGAGCCGCTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGAGCACATCAACACCGAGATCTCCGAGGCCGTGCTG
GGCCTGGACGCTTCCGAGCAGGCCTTCCTGGACAAGACGCTGATCGACCTGGACGGTACCGACAACAAGAGCCGCCTGGG
CGCGAACGCCATGCTGGCCGTCTCCATGGCCGTGGCCCGCGCCGCCGCCGAAGAGTCCGGCCTGCCGCTGTACCGCTACC
TGGGCGGCATGGGCGGCCTGCAGCTGCCCGTGCCGATGATGAACGTCATCAACGGCGGCGCGCATGCCAACAACAGCCTG
GACCTGCAGGAGTTCATGATCATCCCCGTGGGCGCCCCGAGCTTCCGCGAGGCCGTGCGCTGGGGCGCCGAAGTGTTCCA
CGCCCTCAAGAAGATCATCAACGACAAGGGCATGAGCACCGCCGTGGGCGACGAAGGCGGCTTCGCGCCCAGCGTCGAGA
ACCACGAGGCCGCCATCCAGCTGATCCTGCAGGCCATCGATGCCGCCGGCTACACGGCTGGCGAGCAGATCGCCCTGGGC
CTGGACTGCGCCGCCAGCGAGTTCTACAAGGACGGCCACTACGTGCTGGAAGGCGAAGGCGGCATCCGCCTGACGGCCCA
GCAATGGACCGACATGCTGGCCACCTGGGTGGACAAGTACCCCATCATCTCCATCGAGGACGGCATGGCCGAGGGCGACT
GGGATGGCTGGAAGCACCTGACCGAGCGCCTGGGCAAGAACGTGCAGCTGGTGGGCGACGACCTGTTCGTCACCAACACC
AGGATCCTGAAGGAAGGCATCGACAAGGACATCGGCAACTCGATCCTCATCAAGATCAACCAGATCGGCACGCTGACCGA
GACCTTCGCCGCCATCGAGATGGCCAAGCGCGCGGGCTACACCGCAGTGATCTCGCACCGCTCGGGCGAGACGGAAGACT
CCACCATCGCCGACATCGCCGTGGGCACCAACGCCGGCCAGATCAAGACCGGCTCCCTGTCGCGCTCGGACCGCATCGCC
AAGTACAACCAGTTGCTGCGCATCGAGGAAGACCTGGGCGACATCGCCCACTACCCCGGCCGCGCCGCGTTCTACAACCT
GCGCTGA

Upstream 100 bases:

>100_bases
ATAAACCCTCGCCTTGCGGACGCAGACGGTGCATTGGAGAGTCCATGCTACGGCGCTGCCTGCCGCTCATCGATAATCTT
TCAAAACTGGAGAACCTGTA

Downstream 100 bases:

>100_bases
GCAGGGCCAACTCCGGTCCCGTACCGGCCCCGCCCTCTTCCGATCCACGTTCCCGCGGCGCCTGCGCACCATGAATCCCC
GTATCGTCCCCCTGGTGCTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAVL
GLDASEQAFLDKTLIDLDGTDNKSRLGANAMLAVSMAVARAAAEESGLPLYRYLGGMGGLQLPVPMMNVINGGAHANNSL
DLQEFMIIPVGAPSFREAVRWGAEVFHALKKIINDKGMSTAVGDEGGFAPSVENHEAAIQLILQAIDAAGYTAGEQIALG
LDCAASEFYKDGHYVLEGEGGIRLTAQQWTDMLATWVDKYPIISIEDGMAEGDWDGWKHLTERLGKNVQLVGDDLFVTNT
RILKEGIDKDIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIA
KYNQLLRIEEDLGDIAHYPGRAAFYNLR

Sequences:

>Translated_428_residues
MSAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAVL
GLDASEQAFLDKTLIDLDGTDNKSRLGANAMLAVSMAVARAAAEESGLPLYRYLGGMGGLQLPVPMMNVINGGAHANNSL
DLQEFMIIPVGAPSFREAVRWGAEVFHALKKIINDKGMSTAVGDEGGFAPSVENHEAAIQLILQAIDAAGYTAGEQIALG
LDCAASEFYKDGHYVLEGEGGIRLTAQQWTDMLATWVDKYPIISIEDGMAEGDWDGWKHLTERLGKNVQLVGDDLFVTNT
RILKEGIDKDIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIA
KYNQLLRIEEDLGDIAHYPGRAAFYNLR
>Mature_427_residues
SAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAVLG
LDASEQAFLDKTLIDLDGTDNKSRLGANAMLAVSMAVARAAAEESGLPLYRYLGGMGGLQLPVPMMNVINGGAHANNSLD
LQEFMIIPVGAPSFREAVRWGAEVFHALKKIINDKGMSTAVGDEGGFAPSVENHEAAIQLILQAIDAAGYTAGEQIALGL
DCAASEFYKDGHYVLEGEGGIRLTAQQWTDMLATWVDKYPIISIEDGMAEGDWDGWKHLTERLGKNVQLVGDDLFVTNTR
ILKEGIDKDIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIAK
YNQLLRIEEDLGDIAHYPGRAAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=52.6315789473684, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50.2325581395349, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.6279069767442, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.6279069767442, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.4953271028037, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=25.8160237388724, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=25.8160237388724, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=25.8160237388724, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=64.7058823529412, Blast_Score=556, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=51.7162471395881, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=51.7162471395881, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=198, Percent_Identity=45.959595959596, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=49.537037037037, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.729792147806, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.729792147806, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.729792147806, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=49.537037037037, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=50, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=50, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=50, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=50, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=50, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=50, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45853; Mature: 45722

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAVLGLDASEQAFLDKTLIDLDGTDNKSRLGANAMLAVSMAVAR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHCCCHHHHHHHHHHHH
AAAEESGLPLYRYLGGMGGLQLPVPMMNVINGGAHANNSLDLQEFMIIPVGAPSFREAVR
HHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
WGAEVFHALKKIINDKGMSTAVGDEGGFAPSVENHEAAIQLILQAIDAAGYTAGEQIALG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAASEFYKDGHYVLEGEGGIRLTAQQWTDMLATWVDKYPIISIEDGMAEGDWDGWKHL
CHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHH
TERLGKNVQLVGDDLFVTNTRILKEGIDKDIGNSILIKINQIGTLTETFAAIEMAKRAGY
HHHHCCCEEEECCCEEEEHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
TAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIAKYNQLLRIEEDLGDIAHYPG
EEEEECCCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RAAFYNLR
CCEEECCC
>Mature Secondary Structure 
SAIVDIVGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAVLGLDASEQAFLDKTLIDLDGTDNKSRLGANAMLAVSMAVAR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHCCCHHHHHHHHHHHH
AAAEESGLPLYRYLGGMGGLQLPVPMMNVINGGAHANNSLDLQEFMIIPVGAPSFREAVR
HHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
WGAEVFHALKKIINDKGMSTAVGDEGGFAPSVENHEAAIQLILQAIDAAGYTAGEQIALG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAASEFYKDGHYVLEGEGGIRLTAQQWTDMLATWVDKYPIISIEDGMAEGDWDGWKHL
CHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHH
TERLGKNVQLVGDDLFVTNTRILKEGIDKDIGNSILIKINQIGTLTETFAAIEMAKRAGY
HHHHCCCEEEECCCEEEEHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
TAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIAKYNQLLRIEEDLGDIAHYPG
EEEEECCCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RAAFYNLR
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA