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The map label for this gene is amiC [H]

Identifier: 120609992

GI number: 120609992

Start: 1431926

End: 1433479

Strand: Reverse

Name: amiC [H]

Synonym: Aave_1305

Alternate gene names: 120609992

Gene position: 1433479-1431926 (Counterclockwise)

Preceding gene: 120609993

Following gene: 120609991

Centisome position: 26.78

GC content: 71.81

Gene sequence:

>1554_bases
ATGAGCGAAGACGCCTCCCCCCTCCTCCACCCACCTGCCGCCTCCTTGCCCCCGCCCCATGGCCCATCGCGGCGCGGGCT
GCTGCGCGCCGGGTCCCTGGTGCTCCTGCTCGGCTCCGCGCAGATCGCGCGCGGTGCCAGCATCGTGGCGGTGCGCGTAT
GGCCGGCGCAGGACTACTCCCGCGTGACCATCGAATCGGACAAGCAGCTCTCGGCCAAGCAGTTCTTCGTGGCCACGCCG
CCGCGGCTGGCGGTGGACATCGAGGGCATCGACCTGAACCCCGAACTGCGCGAACTGGTCGCGAAGGTCAAACCGGATGA
TCCCAACATCGCCGGCATCCGCGTCGGCCAGAACGCTCCGGGCGTCGTGCGGCTGGTGGTGGACCTCAAGCGCGCCGCGC
TGCCGCAGGTCTTCACCCTGCCGCCGATCGCCGCCTACCGGCACCGGCTGGTATTCGACCTCTACCCCGCCGAGCCCGAG
GATCCGCTGGAAACGCTGATCGCCGAGCGCCTGCGGGACGCGGCGCCGGGAGCCGCCTCCGCGGGAGCGCCCGTCCCCGC
GCCGGCCCCGCTGCCGAACATCGCAGCGGCACGGCCGGGAACGGCCGGCGATCCCCTGGGCGACCTGATCGCACAGCACA
ACCAGCCGGCAGGCAAGGCCGCGCCGCCGTCGCCCGGCGGGCTGCCGTCGGCGGCGCCCGGCCCCACGGCACCGGTGGCC
ACGCCCTCGGGCGCGCCAGCGGCGGTTCCCCCGGCAGTGGCCGCCGCGCCCGCCACGCCTCACACCGGCGGCGCGGCGCA
TCCCGCCGCCCCTGCCATCGCCAGGGCGACCGACCGGCTCATCATCATCGCCCTCGACCCGGGCCATGGCGGAGAAGACC
CGGGTGCCATCGGCCCCGGCGGAACACGCGAGAAGGACGTGGTCCTCAAGATCGCATTCCTGCTGCGCGACCGCATCAAC
GCGACCACCGTCGGGGGCAATCCGATGCGCGCTTTCATGACCCGCGACGCGGATTTTTTCGTGCCGTTGGGCGTGCGGGT
GGACAAGGCGCGCCGCGTGCAGGCGGACCTGTTCGTCAGCATCCATGCCGATGCCTTCACCACGCCGGCCGCCCGCGGCG
CGAGCGTCTTCGCACTGAGCCAGAGCGGCGCCTCCAGCTCGGCCGCGCGCTGGCTGGCCAACAAGGAAAACCAATCCGAC
CTCGTCGGAGGCCTGAACGTGCGCAGCCAGGACAGGCATGTGCAGAGCGCCCTGCTCGACATGAGCACCACCGCGCAGAT
CAACGACAGTCTCAAGCTCGGCAGCGTGCTGCTGGGCGAGATCGGCAACATGGCCAAGCTCCACAAGCCGCGGGTCGAGC
AGGCCGGCTTCGCGGTGCTCAAGGCGCCCGACATCCCCAGCGTGCTCGTGGAAACGGCCTTCATCAGCAACCCGGAGGAA
GAGGCCAAGCTCCGCACGGCGGCCTACCAGCAGCAACTGGCGGATGCGCTGATGCGCGGCATCACCCGCTATTTCGCGAA
GAACCCGCCGCTCGCGCGCAGCCGGTCCGTCTGA

Upstream 100 bases:

>100_bases
CCGCCAACGGCGCGTACGATGGCGACGGCACGGCCCGGCTGGCCCTGCTGCGCGCCGGCACACCCCTTGGCCGCACCCTG
GTGCAAGCACTCCCCGCAGC

Downstream 100 bases:

>100_bases
AATCCATGGCTCCGGGCCGGCCGGCAGCCGCAGGCCGCCGGGGTGTATTCAGGCATTGCCTTGCGGCGCCGGCTGGGCCA
CAGTTCCATCCACCCACGAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSEDASPLLHPPAASLPPPHGPSRRGLLRAGSLVLLLGSAQIARGASIVAVRVWPAQDYSRVTIESDKQLSAKQFFVATP
PRLAVDIEGIDLNPELRELVAKVKPDDPNIAGIRVGQNAPGVVRLVVDLKRAALPQVFTLPPIAAYRHRLVFDLYPAEPE
DPLETLIAERLRDAAPGAASAGAPVPAPAPLPNIAAARPGTAGDPLGDLIAQHNQPAGKAAPPSPGGLPSAAPGPTAPVA
TPSGAPAAVPPAVAAAPATPHTGGAAHPAAPAIARATDRLIIIALDPGHGGEDPGAIGPGGTREKDVVLKIAFLLRDRIN
ATTVGGNPMRAFMTRDADFFVPLGVRVDKARRVQADLFVSIHADAFTTPAARGASVFALSQSGASSSAARWLANKENQSD
LVGGLNVRSQDRHVQSALLDMSTTAQINDSLKLGSVLLGEIGNMAKLHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEE
EAKLRTAAYQQQLADALMRGITRYFAKNPPLARSRSV

Sequences:

>Translated_517_residues
MSEDASPLLHPPAASLPPPHGPSRRGLLRAGSLVLLLGSAQIARGASIVAVRVWPAQDYSRVTIESDKQLSAKQFFVATP
PRLAVDIEGIDLNPELRELVAKVKPDDPNIAGIRVGQNAPGVVRLVVDLKRAALPQVFTLPPIAAYRHRLVFDLYPAEPE
DPLETLIAERLRDAAPGAASAGAPVPAPAPLPNIAAARPGTAGDPLGDLIAQHNQPAGKAAPPSPGGLPSAAPGPTAPVA
TPSGAPAAVPPAVAAAPATPHTGGAAHPAAPAIARATDRLIIIALDPGHGGEDPGAIGPGGTREKDVVLKIAFLLRDRIN
ATTVGGNPMRAFMTRDADFFVPLGVRVDKARRVQADLFVSIHADAFTTPAARGASVFALSQSGASSSAARWLANKENQSD
LVGGLNVRSQDRHVQSALLDMSTTAQINDSLKLGSVLLGEIGNMAKLHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEE
EAKLRTAAYQQQLADALMRGITRYFAKNPPLARSRSV
>Mature_516_residues
SEDASPLLHPPAASLPPPHGPSRRGLLRAGSLVLLLGSAQIARGASIVAVRVWPAQDYSRVTIESDKQLSAKQFFVATPP
RLAVDIEGIDLNPELRELVAKVKPDDPNIAGIRVGQNAPGVVRLVVDLKRAALPQVFTLPPIAAYRHRLVFDLYPAEPED
PLETLIAERLRDAAPGAASAGAPVPAPAPLPNIAAARPGTAGDPLGDLIAQHNQPAGKAAPPSPGGLPSAAPGPTAPVAT
PSGAPAAVPPAVAAAPATPHTGGAAHPAAPAIARATDRLIIIALDPGHGGEDPGAIGPGGTREKDVVLKIAFLLRDRINA
TTVGGNPMRAFMTRDADFFVPLGVRVDKARRVQADLFVSIHADAFTTPAARGASVFALSQSGASSSAARWLANKENQSDL
VGGLNVRSQDRHVQSALLDMSTTAQINDSLKLGSVLLGEIGNMAKLHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEEE
AKLRTAAYQQQLADALMRGITRYFAKNPPLARSRSV

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=493, Percent_Identity=37.525354969574, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=49.5535714285714, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1790611, Length=251, Percent_Identity=45.4183266932271, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 53717; Mature: 53586

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDASPLLHPPAASLPPPHGPSRRGLLRAGSLVLLLGSAQIARGASIVAVRVWPAQDYS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEECCHHHHCCCEEEEEEEECCCCCC
RVTIESDKQLSAKQFFVATPPRLAVDIEGIDLNPELRELVAKVKPDDPNIAGIRVGQNAP
EEEECCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCC
GVVRLVVDLKRAALPQVFTLPPIAAYRHRLVFDLYPAEPEDPLETLIAERLRDAAPGAAS
CHHHHHHHHHHHHCCCEECCCCHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
AGAPVPAPAPLPNIAAARPGTAGDPLGDLIAQHNQPAGKAAPPSPGGLPSAAPGPTAPVA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPSGAPAAVPPAVAAAPATPHTGGAAHPAAPAIARATDRLIIIALDPGHGGEDPGAIGPG
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCC
GTREKDVVLKIAFLLRDRINATTVGGNPMRAFMTRDADFFVPLGVRVDKARRVQADLFVS
CCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCEEEEECCEECCHHHEEEEEEEE
IHADAFTTPAARGASVFALSQSGASSSAARWLANKENQSDLVGGLNVRSQDRHVQSALLD
EECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHH
MSTTAQINDSLKLGSVLLGEIGNMAKLHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEE
CCCHHHCCCCHHHHHHHHHHHCCHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHCCCCHH
EAKLRTAAYQQQLADALMRGITRYFAKNPPLARSRSV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEDASPLLHPPAASLPPPHGPSRRGLLRAGSLVLLLGSAQIARGASIVAVRVWPAQDYS
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEECCHHHHCCCEEEEEEEECCCCCC
RVTIESDKQLSAKQFFVATPPRLAVDIEGIDLNPELRELVAKVKPDDPNIAGIRVGQNAP
EEEECCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCC
GVVRLVVDLKRAALPQVFTLPPIAAYRHRLVFDLYPAEPEDPLETLIAERLRDAAPGAAS
CHHHHHHHHHHHHCCCEECCCCHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
AGAPVPAPAPLPNIAAARPGTAGDPLGDLIAQHNQPAGKAAPPSPGGLPSAAPGPTAPVA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPSGAPAAVPPAVAAAPATPHTGGAAHPAAPAIARATDRLIIIALDPGHGGEDPGAIGPG
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCC
GTREKDVVLKIAFLLRDRINATTVGGNPMRAFMTRDADFFVPLGVRVDKARRVQADLFVS
CCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCEEEEECCEECCHHHEEEEEEEE
IHADAFTTPAARGASVFALSQSGASSSAARWLANKENQSDLVGGLNVRSQDRHVQSALLD
EECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHH
MSTTAQINDSLKLGSVLLGEIGNMAKLHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEE
CCCHHHCCCCHHHHHHHHHHHCCHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHCCCCHH
EAKLRTAAYQQQLADALMRGITRYFAKNPPLARSRSV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]