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The map label for this gene is nuoM [H]

Identifier: 120609962

GI number: 120609962

Start: 1399445

End: 1400920

Strand: Direct

Name: nuoM [H]

Synonym: Aave_1275

Alternate gene names: 120609962

Gene position: 1399445-1400920 (Clockwise)

Preceding gene: 120609961

Following gene: 120609963

Centisome position: 26.14

GC content: 65.58

Gene sequence:

>1476_bases
ATGGGTTTGTTGAGTCTTGCCATCTGGACGCCGATCGCCTTCGGTGCCCTGCTGCTGGCCTTCGGGCGCGACGATCAGGC
GCGCGCCGTCCGCTGGATCGCGCTGATCGGCTCGCTGATCGGCCTCGCCGTGACGCTGCCGCTGCTCAGCGGTTTCGACA
ACGGCACGGCCGCCGCGCAGTTCGTGGAGAAGCTGCTCTGGATCGAGCGCTTCAACGTGCACTACCACCTGGGCCTGGAC
GGCATCTCGTTCTGGTTCGTGCCGCTGACGGCCTTCATCACGGTGATCGTGGTGATCGCGTCGTGGGAGTCGATCACCGA
GCGCGTGAACCAGTACATGGGCGCGTTCCTGATCCTCTCGGGCCTCATGATCGGCGTGTTCAGCGCCCTCGACGGCCTGC
TGTTCTACGTGTTCTTCGAGGCCACGCTGATCCCGATGTACCTGATCATCGGCATCTGGGGCGGCCCGAACAAGATCTAC
GCGGCCTTCAAGTTCTTCCTCTACACGCTGCTCGGCTCGCTGCTGATGCTGATCGCGCTGGTGTACCTGTACAACCAGTC
CGGCGGCAGCTTCGACATCGCCACCTGGCACCAGCTGCCGCTGTCGGCCCGCGCCCAGACGCTGCTGTTCTTCGCCTTCT
TCGCGGCCTTCGCCGTGAAGGTGCCGATGTGGCCGGTGCACACCTGGCTGCCGGACGTGCACGTGGAGGCGCCCACGGGC
GGCTCCGCCGTGCTGGCGGCCATCATGCTGAAGCTGGGCGCCTACGGCTTCCTGCGCTTCTCGATGCCCATCGCTCCCGA
TGCCGCGCGCCACTGGGCGTGGCTGATGATCGCGCTGTCGCTGATCGCGGTGATCTACGTGGGCCTCGTGGCCATGGTGC
AGAAGGACATGAAGAAGCTGGTGGCGTATTCGTCCGTGGCGCACATGGGCTTCGTGACGCTGGGCTTCTTCATCTTCAAC
GACCTGGGCGTCTCGGGCGGCCTGGTGCAGATGATCGCCCACGGCTTCGTGTCGGGCGCGATGTTCCTCTCGATCGGCGT
GCTGTACGACCGCGTGCACTCGCGGGAGATCGCGGCCTACGGCGGCGTTGTGAACACCATGCCGAAGTTCGCGGCCTTCG
CGCTGCTGTTCGCGATGGCCAACTGCGGCCTTCCGGGAACGGCAGGCTTCGTGGGCGAGTGGATGGTGATCCTGGGCGCC
GTGAAGGCGAACTTCTGGATCGGCTTGGCTGCAGCCACCGCGCTGATCTTCGGCGCCGCCTACACGCTGTGGATGTTCAA
GCGCGTCTATCTCGGCCCGGTGGGCAACGACAACGTGCGCGGCCTGCTGGACATCAGCGCCCGCGAATTCCTGGTGCTGG
GCGTGCTGGCCGTGGCCGTGCTGTACATGGGCCTGTATCCGCGCCCCTTCACCGACGTGATGGACGTGTCGGTGGCCGAG
CTGCTCAAGCACGTGGCGCAGACCAAGCTCCATTGA

Upstream 100 bases:

>100_bases
CAGTCGGGCTACATCTACCACTATGCGCTGGTGATGATCCTGGGCGTGTTCGGGCTCATGACGTACTTCGTGTGGCTCAA
CAAGTAGAGGAACAAGAAAA

Downstream 100 bases:

>100_bases
ACCGACTGACAACGAGAGACCGAGATGATTGACAACATCAGCTGGCTGGCGGTGTACCCGGAGATCGTGCTCCTGGTGAT
GGCCTGCGTGATCGCCCTGG

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MGLLSLAIWTPIAFGALLLAFGRDDQARAVRWIALIGSLIGLAVTLPLLSGFDNGTAAAQFVEKLLWIERFNVHYHLGLD
GISFWFVPLTAFITVIVVIASWESITERVNQYMGAFLILSGLMIGVFSALDGLLFYVFFEATLIPMYLIIGIWGGPNKIY
AAFKFFLYTLLGSLLMLIALVYLYNQSGGSFDIATWHQLPLSARAQTLLFFAFFAAFAVKVPMWPVHTWLPDVHVEAPTG
GSAVLAAIMLKLGAYGFLRFSMPIAPDAARHWAWLMIALSLIAVIYVGLVAMVQKDMKKLVAYSSVAHMGFVTLGFFIFN
DLGVSGGLVQMIAHGFVSGAMFLSIGVLYDRVHSREIAAYGGVVNTMPKFAAFALLFAMANCGLPGTAGFVGEWMVILGA
VKANFWIGLAAATALIFGAAYTLWMFKRVYLGPVGNDNVRGLLDISAREFLVLGVLAVAVLYMGLYPRPFTDVMDVSVAE
LLKHVAQTKLH

Sequences:

>Translated_491_residues
MGLLSLAIWTPIAFGALLLAFGRDDQARAVRWIALIGSLIGLAVTLPLLSGFDNGTAAAQFVEKLLWIERFNVHYHLGLD
GISFWFVPLTAFITVIVVIASWESITERVNQYMGAFLILSGLMIGVFSALDGLLFYVFFEATLIPMYLIIGIWGGPNKIY
AAFKFFLYTLLGSLLMLIALVYLYNQSGGSFDIATWHQLPLSARAQTLLFFAFFAAFAVKVPMWPVHTWLPDVHVEAPTG
GSAVLAAIMLKLGAYGFLRFSMPIAPDAARHWAWLMIALSLIAVIYVGLVAMVQKDMKKLVAYSSVAHMGFVTLGFFIFN
DLGVSGGLVQMIAHGFVSGAMFLSIGVLYDRVHSREIAAYGGVVNTMPKFAAFALLFAMANCGLPGTAGFVGEWMVILGA
VKANFWIGLAAATALIFGAAYTLWMFKRVYLGPVGNDNVRGLLDISAREFLVLGVLAVAVLYMGLYPRPFTDVMDVSVAE
LLKHVAQTKLH
>Mature_490_residues
GLLSLAIWTPIAFGALLLAFGRDDQARAVRWIALIGSLIGLAVTLPLLSGFDNGTAAAQFVEKLLWIERFNVHYHLGLDG
ISFWFVPLTAFITVIVVIASWESITERVNQYMGAFLILSGLMIGVFSALDGLLFYVFFEATLIPMYLIIGIWGGPNKIYA
AFKFFLYTLLGSLLMLIALVYLYNQSGGSFDIATWHQLPLSARAQTLLFFAFFAAFAVKVPMWPVHTWLPDVHVEAPTGG
SAVLAAIMLKLGAYGFLRFSMPIAPDAARHWAWLMIALSLIAVIYVGLVAMVQKDMKKLVAYSSVAHMGFVTLGFFIFND
LGVSGGLVQMIAHGFVSGAMFLSIGVLYDRVHSREIAAYGGVVNTMPKFAAFALLFAMANCGLPGTAGFVGEWMVILGAV
KANFWIGLAAATALIFGAAYTLWMFKRVYLGPVGNDNVRGLLDISAREFLVLGVLAVAVLYMGLYPRPFTDVMDVSVAEL
LKHVAQTKLH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=34.5108695652174, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI251831117, Length=387, Percent_Identity=24.2894056847545, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788613, Length=473, Percent_Identity=37.8435517970402, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1788827, Length=273, Percent_Identity=28.9377289377289, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788831, Length=304, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788829, Length=429, Percent_Identity=27.5058275058275, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788614, Length=463, Percent_Identity=27.2138228941685, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI145693160, Length=365, Percent_Identity=24.3835616438356, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367154, Length=241, Percent_Identity=27.8008298755187, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53546; Mature: 53415

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLSLAIWTPIAFGALLLAFGRDDQARAVRWIALIGSLIGLAVTLPLLSGFDNGTAAAQ
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FVEKLLWIERFNVHYHLGLDGISFWFVPLTAFITVIVVIASWESITERVNQYMGAFLILS
HHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLMIGVFSALDGLLFYVFFEATLIPMYLIIGIWGGPNKIYAAFKFFLYTLLGSLLMLIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VYLYNQSGGSFDIATWHQLPLSARAQTLLFFAFFAAFAVKVPMWPVHTWLPDVHVEAPTG
HHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCC
GSAVLAAIMLKLGAYGFLRFSMPIAPDAARHWAWLMIALSLIAVIYVGLVAMVQKDMKKL
HHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAYSSVAHMGFVTLGFFIFNDLGVSGGLVQMIAHGFVSGAMFLSIGVLYDRVHSREIAAY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGVVNTMPKFAAFALLFAMANCGLPGTAGFVGEWMVILGAVKANFWIGLAAATALIFGAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTLWMFKRVYLGPVGNDNVRGLLDISAREFLVLGVLAVAVLYMGLYPRPFTDVMDVSVAE
HHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LLKHVAQTKLH
HHHHHHHHCCC
>Mature Secondary Structure 
GLLSLAIWTPIAFGALLLAFGRDDQARAVRWIALIGSLIGLAVTLPLLSGFDNGTAAAQ
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FVEKLLWIERFNVHYHLGLDGISFWFVPLTAFITVIVVIASWESITERVNQYMGAFLILS
HHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLMIGVFSALDGLLFYVFFEATLIPMYLIIGIWGGPNKIYAAFKFFLYTLLGSLLMLIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VYLYNQSGGSFDIATWHQLPLSARAQTLLFFAFFAAFAVKVPMWPVHTWLPDVHVEAPTG
HHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCC
GSAVLAAIMLKLGAYGFLRFSMPIAPDAARHWAWLMIALSLIAVIYVGLVAMVQKDMKKL
HHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAYSSVAHMGFVTLGFFIFNDLGVSGGLVQMIAHGFVSGAMFLSIGVLYDRVHSREIAAY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGVVNTMPKFAAFALLFAMANCGLPGTAGFVGEWMVILGAVKANFWIGLAAATALIFGAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTLWMFKRVYLGPVGNDNVRGLLDISAREFLVLGVLAVAVLYMGLYPRPFTDVMDVSVAE
HHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LLKHVAQTKLH
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]