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The map label for this gene is grpE

Identifier: 120609914

GI number: 120609914

Start: 1353628

End: 1354197

Strand: Reverse

Name: grpE

Synonym: Aave_1227

Alternate gene names: 120609914

Gene position: 1354197-1353628 (Counterclockwise)

Preceding gene: 120609920

Following gene: 120609913

Centisome position: 25.3

GC content: 70.0

Gene sequence:

>570_bases
ATGTCCGATCAACAGCATTCAGCCCCCCAGAACGCCGCGGCGACGGCGTCTCCGTCCGACAGCCCCGAAGCCGTGGAGGC
GACCATGGCCGCGCATGCGGCGGACGAGCTGGGCCGCCTGCAGAACGAACTGGCCGAGCTCAAGGCCAAGAGCGCCGACC
TCGCCGACCAGTTCCTGCGCGCCAAGGCCGAAGCCGAGAACGCCCGCCGCCGCGCGGACGAGGAAGTCTCCAAGGCACGC
AAGTTCGGCATCGAGAGCTTCGCCGAGAGCCTGCTGCCCGTGGCCGACAGCCTGACGGCGGCGCTCGCCATCAAGGACGC
CACGATCGAGCAGCTGCGCGAAGGCACCGACGCCACGCTGCGCCAGCTCACCTCCGCCCTCGAGCGCAACAAGGTGCTGG
CGATCCAGCCGGGCGCCGGCGAGAAGTTCGACCCCCACCAGCACCAGGCCATCAGCATGGTGCCCGCCGAGCAGGAGCCC
AACACCATCGTGTCCGTGCTGCAGAAAGGCTACGTCATCGCCGACCGGGTGCTGCGCCCGGCCCTCGTCACGGTGGCGGC
ACCGAAGTGA

Upstream 100 bases:

>100_bases
GGCAGATGCCGGAATTTGGCGGGACTTCGCTTGAAATCCCTCCCTTTGCACCCAGGTGCCGTGCGTCCATCGTTCATTCA
CCTACACCAGAACAACCGAC

Downstream 100 bases:

>100_bases
CGCCCCCGTCCGGCCCTCGCCGCCCGGTACGCCGCGGAAAGAAATTCCCCGGGCCGACGGCTTGAAGCCGGACAAGTTAT
CCACAAGTTACTGGCTATCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSDQQHSAPQNAAATASPSDSPEAVEATMAAHAADELGRLQNELAELKAKSADLADQFLRAKAEAENARRRADEEVSKAR
KFGIESFAESLLPVADSLTAALAIKDATIEQLREGTDATLRQLTSALERNKVLAIQPGAGEKFDPHQHQAISMVPAEQEP
NTIVSVLQKGYVIADRVLRPALVTVAAPK

Sequences:

>Translated_189_residues
MSDQQHSAPQNAAATASPSDSPEAVEATMAAHAADELGRLQNELAELKAKSADLADQFLRAKAEAENARRRADEEVSKAR
KFGIESFAESLLPVADSLTAALAIKDATIEQLREGTDATLRQLTSALERNKVLAIQPGAGEKFDPHQHQAISMVPAEQEP
NTIVSVLQKGYVIADRVLRPALVTVAAPK
>Mature_188_residues
SDQQHSAPQNAAATASPSDSPEAVEATMAAHAADELGRLQNELAELKAKSADLADQFLRAKAEAENARRRADEEVSKARK
FGIESFAESLLPVADSLTAALAIKDATIEQLREGTDATLRQLTSALERNKVLAIQPGAGEKFDPHQHQAISMVPAEQEPN
TIVSVLQKGYVIADRVLRPALVTVAAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=156, Percent_Identity=35.2564102564103, Blast_Score=87, Evalue=9e-18,
Organism=Homo sapiens, GI40255109, Length=170, Percent_Identity=32.9411764705882, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=34.010152284264, Blast_Score=100, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=32.7044025157233, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=159, Percent_Identity=29.559748427673, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653432, Length=187, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_ACIAC (A1TLI0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969592.1
- ProteinModelPortal:   A1TLI0
- SMR:   A1TLI0
- STRING:   A1TLI0
- GeneID:   4665610
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1227
- NMPDR:   fig|397945.5.peg.1077
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   A1TLI0
- ProtClustDB:   PRK14139
- BioCyc:   AAVE397945:AAVE_1227-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20186; Mature: 20055

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQQHSAPQNAAATASPSDSPEAVEATMAAHAADELGRLQNELAELKAKSADLADQFLR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAEAENARRRADEEVSKARKFGIESFAESLLPVADSLTAALAIKDATIEQLREGTDATL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RQLTSALERNKVLAIQPGAGEKFDPHQHQAISMVPAEQEPNTIVSVLQKGYVIADRVLRP
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHH
ALVTVAAPK
HHHHCCCCC
>Mature Secondary Structure 
SDQQHSAPQNAAATASPSDSPEAVEATMAAHAADELGRLQNELAELKAKSADLADQFLR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAEAENARRRADEEVSKARKFGIESFAESLLPVADSLTAALAIKDATIEQLREGTDATL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RQLTSALERNKVLAIQPGAGEKFDPHQHQAISMVPAEQEPNTIVSVLQKGYVIADRVLRP
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHH
ALVTVAAPK
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA