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The map label for this gene is proP [H]

Identifier: 120609751

GI number: 120609751

Start: 1160241

End: 1161569

Strand: Direct

Name: proP [H]

Synonym: Aave_1058

Alternate gene names: 120609751

Gene position: 1160241-1161569 (Clockwise)

Preceding gene: 120609749

Following gene: 120609752

Centisome position: 21.68

GC content: 69.75

Gene sequence:

>1329_bases
ATGACTTCCTCTTCTTCCGCTGCCGCGAGCGCCGACGCCGCGCAGCCCCATCGCCCGCTCACGCGCAGCGACTACAAGAC
CCTGGGCCTTTCCGCGCTGGGCGGCACGCTCGAGTTCTACGATTTCGTCATCTACGTGTTCTTCGCCACGGTGGTGAGCG
GCCTGTTCTTCCCGGCCGACATGCCCGACTGGCTGCGCCAGCTGCAGACGTTCGGCATCTTCGCGGCGGGCTACCTGGCC
CGGCCGATCGGCGGCATCGTGATCGCCCACTTCGGCGACAAGCTGGGCCGCAAGCGCATGTTCAGCCTGTCGATCTTCCT
GATGGCGGTGCCGACGCTGGTGATCGGCCTGCTGCCCACCTACGCGAGCATCGGCGTGGCGGCGCCGCTGCTGCTGCTGG
CGATGCGCGTGCTGCAGGGCGCGGCCATCGGCGGCGAGATGCCCGGGGCCTGGGTGTTCGTGGCCGAACACGTGCCGAAG
AACCGCTACGGCCTGGGCGTGGGCACGCTGACGGCCGGCATCACGGGCGGCATCCTGCTGGGCTCGCTGGTGGCGATCGC
CATCAACCGCACGTACACGCCCGCCGAGGTGCAGGGCTTCGCCTGGCGCATTCCGTTCATCCTCGGCGGCGTGTTCGGCC
TGGTGTCGGTGTACCTGCGCCGCTTCCTGCACGAGACGCCGGTGTTCCAGGAGCTGGCCGCGCGCCGCGCCGTGGCGCGC
GAATTGCCGCTCAAGACCATCTTCCGCGACCACAAGGGCGCGATGCTGCTGGTGGCGCTGATGACCTGGGTGCTCTCCAC
CGCCGTGGTGGTGGTGGTGCTGATCACGCCGTCGTACCTGCAGAAGGTGTTCCACCTGCCCGCCACGCTGGCCCTGGAGG
CCAACGCCGTGGCCACGCTCACGCTGACCATCGGCTGCGTGGTGATGGGCTGGGCCACCGACCGCTTCGGCACCCGCGCA
GTGATGCTGGCGGGCTGGGGCGGCCTGTTCCTGACCTCGTACCTGTTCTACCTGGGCCTGCCCGGCACGCCGGAGACGCT
GGTGCGCAACTATGCGCTGGTGGGCTTTTTCGTGGGCTCGATCGCGCTGCTGCCGATCGTGGGCGTGCGCGCCTTCCCGC
CGGACGTGCGCTTCTCGGGCCTTTCGTTCTCCTACAACATGGCCTATGCGGTGTTCGGCGGCATCACGCCGGTGCTGCTG
ACGGTGTGGATGCAGCGCGACCCGATGGCGCCCGCGCACTACGTGGCGGCCATGGGGCTGCTGGGCTTCGTGCTGGGCTT
CGTACCGCTGGCCTCGCGCGGCTGGCAGCCTGCGCCCGCGCGGGCCTGA

Upstream 100 bases:

>100_bases
GCCACGCTCCGCTCCGGTCCGGCGGCCGCAAGAGAAAGCCCGGGCGCCGCCGCATGCATGGCCGAGTCCGGGCCGCACCC
GTGCATTTCCGGACCACGCC

Downstream 100 bases:

>100_bases
ACGGGCCCGATGAGGGCGGGCGGCGCCGTTTTCGGGCGCAGGCCCTGCCACAATGCCGTGCATGACGGACCCAGCTACCT
TCCCTTCCACGGATGACTCC

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSSSSAAASADAAQPHRPLTRSDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPADMPDWLRQLQTFGIFAAGYLA
RPIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPTYASIGVAAPLLLLAMRVLQGAAIGGEMPGAWVFVAEHVPK
NRYGLGVGTLTAGITGGILLGSLVAIAINRTYTPAEVQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVAR
ELPLKTIFRDHKGAMLLVALMTWVLSTAVVVVVLITPSYLQKVFHLPATLALEANAVATLTLTIGCVVMGWATDRFGTRA
VMLAGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGSIALLPIVGVRAFPPDVRFSGLSFSYNMAYAVFGGITPVLL
TVWMQRDPMAPAHYVAAMGLLGFVLGFVPLASRGWQPAPARA

Sequences:

>Translated_442_residues
MTSSSSAAASADAAQPHRPLTRSDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPADMPDWLRQLQTFGIFAAGYLA
RPIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPTYASIGVAAPLLLLAMRVLQGAAIGGEMPGAWVFVAEHVPK
NRYGLGVGTLTAGITGGILLGSLVAIAINRTYTPAEVQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVAR
ELPLKTIFRDHKGAMLLVALMTWVLSTAVVVVVLITPSYLQKVFHLPATLALEANAVATLTLTIGCVVMGWATDRFGTRA
VMLAGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGSIALLPIVGVRAFPPDVRFSGLSFSYNMAYAVFGGITPVLL
TVWMQRDPMAPAHYVAAMGLLGFVLGFVPLASRGWQPAPARA
>Mature_441_residues
TSSSSAAASADAAQPHRPLTRSDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPADMPDWLRQLQTFGIFAAGYLAR
PIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPTYASIGVAAPLLLLAMRVLQGAAIGGEMPGAWVFVAEHVPKN
RYGLGVGTLTAGITGGILLGSLVAIAINRTYTPAEVQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVARE
LPLKTIFRDHKGAMLLVALMTWVLSTAVVVVVLITPSYLQKVFHLPATLALEANAVATLTLTIGCVVMGWATDRFGTRAV
MLAGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGSIALLPIVGVRAFPPDVRFSGLSFSYNMAYAVFGGITPVLLT
VWMQRDPMAPAHYVAAMGLLGFVLGFVPLASRGWQPAPARA

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=30.3738317757009, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788292, Length=398, Percent_Identity=30.6532663316583, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=31.358024691358, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789941, Length=413, Percent_Identity=31.2348668280872, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87082231, Length=469, Percent_Identity=23.6673773987207, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=396, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47398; Mature: 47267

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSSSAAASADAAQPHRPLTRSDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPAD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
MPDWLRQLQTFGIFAAGYLARPIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPT
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YASIGVAAPLLLLAMRVLQGAAIGGEMPGAWVFVAEHVPKNRYGLGVGTLTAGITGGILL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
GSLVAIAINRTYTPAEVQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVAR
HHHHHHHHCCCCCCHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELPLKTIFRDHKGAMLLVALMTWVLSTAVVVVVLITPSYLQKVFHLPATLALEANAVATL
HCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCEEH
TLTIGCVVMGWATDRFGTRAVMLAGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGS
HHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IALLPIVGVRAFPPDVRFSGLSFSYNMAYAVFGGITPVLLTVWMQRDPMAPAHYVAAMGL
HHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGFVLGFVPLASRGWQPAPARA
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TSSSSAAASADAAQPHRPLTRSDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPAD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
MPDWLRQLQTFGIFAAGYLARPIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPT
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YASIGVAAPLLLLAMRVLQGAAIGGEMPGAWVFVAEHVPKNRYGLGVGTLTAGITGGILL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
GSLVAIAINRTYTPAEVQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVAR
HHHHHHHHCCCCCCHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELPLKTIFRDHKGAMLLVALMTWVLSTAVVVVVLITPSYLQKVFHLPATLALEANAVATL
HCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCEEH
TLTIGCVVMGWATDRFGTRAVMLAGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGS
HHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IALLPIVGVRAFPPDVRFSGLSFSYNMAYAVFGGITPVLLTVWMQRDPMAPAHYVAAMGL
HHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGFVLGFVPLASRGWQPAPARA
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]