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The map label for this gene is nadE [H]

Identifier: 120609669

GI number: 120609669

Start: 1070313

End: 1072013

Strand: Reverse

Name: nadE [H]

Synonym: Aave_0975

Alternate gene names: 120609669

Gene position: 1072013-1070313 (Counterclockwise)

Preceding gene: 120609670

Following gene: 120609668

Centisome position: 20.03

GC content: 69.84

Gene sequence:

>1701_bases
ATGCTCCGGATCACCCTTGCCCAGCTCAACCTCACCGTGGGCGACATCGAAGGCAACGTGCAGCGGATGACCGACGCCGC
CCGACAGGCGGCGCAGGCCGGCGCCGACCTGGTGGTGTTCACCGAGCTGGCGCTGTGCGGCTACTACCCCGGCGACATGC
TGGAAGAGTCCGGTTTCATGGAGCGCGTGGACGCCGGCATCGCCGCGCTGCAGGACGCTTCGCGCGCCACGCCGGGGCTG
CATTGGGTGGTGGGCACGCCCACGCGCGCGCAGGGCCCCGGCAAGCGCCTGCACAACAGCCTGCTGGTGCTGCGGGACGG
AGCGATCCGCCTCACCTACGCCAAGCAATTGCTGCCCACCTACAACATCTTCGACGAACGCCGCCATTTCGAGCCCGGCC
CCGACGTGGCGCGCGTGCTGCGCATCGGCCACGCGCAGATCGGCTTCCTGGTCTGCGAGGACGGCTGGAACGACGCCGTG
GCCGACTACGCCACCAACCCCTTCCAGCGCCTGGCCGATGCCGCGCCGGACCTGGTGGTGAGCATCAATGCCAGCCCCAG
CCACATCGGCAAGCGCGAGGTCCGGCACCAAGTATTCGGCGACGCCGCGCGGCGCCACGGCCTGCCGGTGGTCTATGTGA
ACCAGGTCGGCGGCCAGGACCAGATCGTCTATGACGGTGCGTCGTTCGCGGCGGAGCCGGAGGCGGGCGTGGTCTTCGAG
GCGCCGCGGTTCGCCGAAGACGTGCGCACCCTGTGCCTGGAGGGCGGGCGCTTCCGCTCCGCGGATGGCGGCCCCCTGCC
GCCCGTGCCGCGGGAGGGCCTGCCCACCATGGACTTCTACCGCCAGCAGATCGTGCTCGGCCTGCGCGACTACGCGCGGC
GCTGCGGCTTCGGGCAGGTGGTGGTCGGCAGTTCGGGCGGTATCGACAGCGCCCTGACCCTGGCGCTCGCCGCCGAGGCG
ATGGGCGCGGAGAATGTCATAGCGGTGACCATGCCCTCGCGCTTTTCCTCCAGCGGATCGGTGGACGATTCGGTGGCGCT
CTGCCGCAACCTCGGCATCCGGCTGCACACGCACCCCATCGCGGACCTCGTGGCGGGCTATGCCGCGCAGTTCGAGGCCA
GCTTCGGCCAGTCGCTCGAAGGACTGCCGCTGGAGAACCTGCAGGCACGCATCCGCGGCACCATCCTGATGGAGTATTCG
AACGCCTTCGGGCACCTGCTGCTCACCACGGGCAACAAGTCGGAGATTTCGGTGGGCTATTGCACGCTCTACGGCGACAC
GAACGGCGGCCTGGGGCTGCTGGGCGATCTCTACAAGACCGAGGTGTTCGAGCTGTCCCGCCACGTCAACGCGCACGCCG
GCCGCGAACTGATTCCCCAGGCCATCATCGAAAAGGAGCCGTCGGCCGAACTGGCTCCCGGCCAGCGGGACGTGGACAGC
CTGCCGCCCTATCCGGTGCTGGACCAGGTGCTCAAGCTCCTCATCGAAGGCGAGGGCCTCTCGCACGCCGAGTATGCCGA
TGCGCGCGACTTCGCCGCCCGCCTGCAGCATGACGAGGCCGGCCGCGCGCTGGTGCACCGGGTGCGCCGGATGATCGCGC
GCAACGAGTACAAGCGCCGCCAGGCGCCGCCCATCCTGCGCGTGCGCGCCAAGGCCTTCGGCAACGGCCGGCAGATGCCG
ATCGCGGCGAAGTACGTCTGA

Upstream 100 bases:

>100_bases
GACGATGAAACCTACCTCGCCTACCTGCGGCAGGTATTCGGCCTGCCGCCCGAGGCCTGAGCCTTTTCGCCTTGCCCCCA
ACAACCTCCAAGGAAACGAC

Downstream 100 bases:

>100_bases
CCGCGCCGGCACGGCTACATTTGCCGCAGTGGCCCTGCAGCCCCGCCGGGCGCGTCCCGCCCGCGGGCTTCCCTCATCGC
ATCCGCATCCATGTACGACA

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MLRITLAQLNLTVGDIEGNVQRMTDAARQAAQAGADLVVFTELALCGYYPGDMLEESGFMERVDAGIAALQDASRATPGL
HWVVGTPTRAQGPGKRLHNSLLVLRDGAIRLTYAKQLLPTYNIFDERRHFEPGPDVARVLRIGHAQIGFLVCEDGWNDAV
ADYATNPFQRLADAAPDLVVSINASPSHIGKREVRHQVFGDAARRHGLPVVYVNQVGGQDQIVYDGASFAAEPEAGVVFE
APRFAEDVRTLCLEGGRFRSADGGPLPPVPREGLPTMDFYRQQIVLGLRDYARRCGFGQVVVGSSGGIDSALTLALAAEA
MGAENVIAVTMPSRFSSSGSVDDSVALCRNLGIRLHTHPIADLVAGYAAQFEASFGQSLEGLPLENLQARIRGTILMEYS
NAFGHLLLTTGNKSEISVGYCTLYGDTNGGLGLLGDLYKTEVFELSRHVNAHAGRELIPQAIIEKEPSAELAPGQRDVDS
LPPYPVLDQVLKLLIEGEGLSHAEYADARDFAARLQHDEAGRALVHRVRRMIARNEYKRRQAPPILRVRAKAFGNGRQMP
IAAKYV

Sequences:

>Translated_566_residues
MLRITLAQLNLTVGDIEGNVQRMTDAARQAAQAGADLVVFTELALCGYYPGDMLEESGFMERVDAGIAALQDASRATPGL
HWVVGTPTRAQGPGKRLHNSLLVLRDGAIRLTYAKQLLPTYNIFDERRHFEPGPDVARVLRIGHAQIGFLVCEDGWNDAV
ADYATNPFQRLADAAPDLVVSINASPSHIGKREVRHQVFGDAARRHGLPVVYVNQVGGQDQIVYDGASFAAEPEAGVVFE
APRFAEDVRTLCLEGGRFRSADGGPLPPVPREGLPTMDFYRQQIVLGLRDYARRCGFGQVVVGSSGGIDSALTLALAAEA
MGAENVIAVTMPSRFSSSGSVDDSVALCRNLGIRLHTHPIADLVAGYAAQFEASFGQSLEGLPLENLQARIRGTILMEYS
NAFGHLLLTTGNKSEISVGYCTLYGDTNGGLGLLGDLYKTEVFELSRHVNAHAGRELIPQAIIEKEPSAELAPGQRDVDS
LPPYPVLDQVLKLLIEGEGLSHAEYADARDFAARLQHDEAGRALVHRVRRMIARNEYKRRQAPPILRVRAKAFGNGRQMP
IAAKYV
>Mature_566_residues
MLRITLAQLNLTVGDIEGNVQRMTDAARQAAQAGADLVVFTELALCGYYPGDMLEESGFMERVDAGIAALQDASRATPGL
HWVVGTPTRAQGPGKRLHNSLLVLRDGAIRLTYAKQLLPTYNIFDERRHFEPGPDVARVLRIGHAQIGFLVCEDGWNDAV
ADYATNPFQRLADAAPDLVVSINASPSHIGKREVRHQVFGDAARRHGLPVVYVNQVGGQDQIVYDGASFAAEPEAGVVFE
APRFAEDVRTLCLEGGRFRSADGGPLPPVPREGLPTMDFYRQQIVLGLRDYARRCGFGQVVVGSSGGIDSALTLALAAEA
MGAENVIAVTMPSRFSSSGSVDDSVALCRNLGIRLHTHPIADLVAGYAAQFEASFGQSLEGLPLENLQARIRGTILMEYS
NAFGHLLLTTGNKSEISVGYCTLYGDTNGGLGLLGDLYKTEVFELSRHVNAHAGRELIPQAIIEKEPSAELAPGQRDVDS
LPPYPVLDQVLKLLIEGEGLSHAEYADARDFAARLQHDEAGRALVHRVRRMIARNEYKRRQAPPILRVRAKAFGNGRQMP
IAAKYV

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61394; Mature: 61394

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRITLAQLNLTVGDIEGNVQRMTDAARQAAQAGADLVVFTELALCGYYPGDMLEESGFM
CEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHCCHH
ERVDAGIAALQDASRATPGLHWVVGTPTRAQGPGKRLHNSLLVLRDGAIRLTYAKQLLPT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCEEEEECCCEEEEEHHHHCCC
YNIFDERRHFEPGPDVARVLRIGHAQIGFLVCEDGWNDAVADYATNPFQRLADAAPDLVV
HHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCHHHHHHHCCCCEEE
SINASPSHIGKREVRHQVFGDAARRHGLPVVYVNQVGGQDQIVYDGASFAAEPEAGVVFE
EECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEE
APRFAEDVRTLCLEGGRFRSADGGPLPPVPREGLPTMDFYRQQIVLGLRDYARRCGFGQV
CCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VVGSSGGIDSALTLALAAEAMGAENVIAVTMPSRFSSSGSVDDSVALCRNLGIRLHTHPI
EECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEECCHH
ADLVAGYAAQFEASFGQSLEGLPLENLQARIRGTILMEYSNAFGHLLLTTGNKSEISVGY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCEEEEEE
CTLYGDTNGGLGLLGDLYKTEVFELSRHVNAHAGRELIPQAIIEKEPSAELAPGQRDVDS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
LPPYPVLDQVLKLLIEGEGLSHAEYADARDFAARLQHDEAGRALVHRVRRMIARNEYKRR
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QAPPILRVRAKAFGNGRQMPIAAKYV
CCCCCEEEEHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MLRITLAQLNLTVGDIEGNVQRMTDAARQAAQAGADLVVFTELALCGYYPGDMLEESGFM
CEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHCCHH
ERVDAGIAALQDASRATPGLHWVVGTPTRAQGPGKRLHNSLLVLRDGAIRLTYAKQLLPT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCEEEEECCCEEEEEHHHHCCC
YNIFDERRHFEPGPDVARVLRIGHAQIGFLVCEDGWNDAVADYATNPFQRLADAAPDLVV
HHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCHHHHHHHCCCCEEE
SINASPSHIGKREVRHQVFGDAARRHGLPVVYVNQVGGQDQIVYDGASFAAEPEAGVVFE
EECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEE
APRFAEDVRTLCLEGGRFRSADGGPLPPVPREGLPTMDFYRQQIVLGLRDYARRCGFGQV
CCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VVGSSGGIDSALTLALAAEAMGAENVIAVTMPSRFSSSGSVDDSVALCRNLGIRLHTHPI
EECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEECCHH
ADLVAGYAAQFEASFGQSLEGLPLENLQARIRGTILMEYSNAFGHLLLTTGNKSEISVGY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCEEEEEE
CTLYGDTNGGLGLLGDLYKTEVFELSRHVNAHAGRELIPQAIIEKEPSAELAPGQRDVDS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
LPPYPVLDQVLKLLIEGEGLSHAEYADARDFAARLQHDEAGRALVHRVRRMIARNEYKRR
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QAPPILRVRAKAFGNGRQMPIAAKYV
CCCCCEEEEHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]