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The map label for this gene is pilJ [H]

Identifier: 120609598

GI number: 120609598

Start: 979524

End: 981761

Strand: Direct

Name: pilJ [H]

Synonym: Aave_0904

Alternate gene names: 120609598

Gene position: 979524-981761 (Clockwise)

Preceding gene: 120609597

Following gene: 120609599

Centisome position: 18.3

GC content: 69.12

Gene sequence:

>2238_bases
ATGTCCGTCGTGAATCCGTTTGCAAAACTCTTCCCCCGCAGGACCGCGGGAGATGCCTCTGCCGGCCAGGACTCGCTCGC
GGCCGCCTCCTACCAGTCGGACGGCATGCAGAGCGTCCAGGGGGAGCCTTCGGCGCTGCCGCCGTCGCCGGAAGGCGATT
TCGCCGAAGAGGATCTGATCTCGCTGCCCCTGCTCGGCCGTGCCACGGCGGCGGCGCACCAGCGCCGGCTGCTCACGCTG
CTGGCCGTGGGCGTGGTGGTGCTGGCGCTGATCGCCGGCTGGGTGCTGAAGCAGGCGGACCGTTCCGCCCAGCAGCTCGC
CGCCACCGGCCAGGCCCTGATGCAGTCCCAGCGGCTGGCGAAGTCGGTCTCGCAGGCACTGGTCGGCAGCCCGCAGGCCT
TCCCCGACGTGGTGGACAGCTCGGGCGTGCTGGTGCGCAACGTGCGCGCGCTGTCGTCCGGCGACGATGACCTGGGCGTG
CAGGCCCTGGGCGAACCGTTCAAGCCCGACCTGGAAGCGATCACGCCGCTGATGGAGCGCGCGGAGCGCAATGCCGGCGT
CGTGATGGGCCAGCAGAAGATCCTCACGCAGGTGGGCGATGCGCTGCGCACCATCAACCGGCAGTCGTCCGACCTGCTGG
AGATCGCCGAGACGATCTCCTCGCTGAAGCTGCAGCAGAACGCGCCCTCCACGGACATCTCCGCGTCCGGCCAGCTCGTG
ATGCTGACACAGCGCATCGGCAAGTCCGCCAACGAGTTCCAGACCACCGAGGGCGTGAGCCCCGAGGCCGTGTTCCTGCT
GGGCAAGGACCTGAATTCCTTCCGCGAGATCGCCCAGGGCATGCTCGACGGCAGCAGCGACCTGCGCCTGTCGGCCACGC
GCGATCCGCAGACGCGCGAGCAGCTCGAGAACCTGATCAAGGTGTACGACCAGACCCGCGTGCAGGCCGGCGCCATCCTC
GGCAACCTGCAGGGCCTGGTGTCCGCCCGCGAGGCGCAGTCCGCCATCATTGCCGACAGCGAGCCGCTGCGCCGCCAACT
CGAAGGCCTGCAGACCAAGCTCTCCGGGCAGACCGGCCTGGGCGCAGGGCAACTGGCCGCCCTGGTGGCCGCCGGCCTGT
TCGTGCTGCTGTGCGGCGTGGGCATTTCCCGCGTCCAGCTGATGGACAGCCGCACCCGCCAGGCCGCTGCCGAAATGCAG
CAGCGCGACGCCCGCCGCCAGGAGCAGGAGGCCAAGCGCGTCAACGACGCCAACCAGGCCGCCATTCTGCGACTCATGAA
CGAACTGCAGTCGGTCGCCGAGGGTGACCTGACGCAGGAAGCCACCGTGACGGAAGACATCACGGGCGCCATCGCCGACT
CGGTGAACTACACGGTGGAAGAGCTCCGCCAACTGGTGGGCAGCGTGCAGAACACGGCCACCCGCGTGGCCCAGACGACG
GCGCAGGTGGACAGCACTTCGACCGAACTGCTGGCCGCCTCCACCGAACAGCTGCGCGAGATCCGCGAAACCGGCCGCTC
GGTGCTCGACATGGCGACCCGAATCAACGAGGTGTCCGCGCAGGCGCAGGAATCGGCCGCGGTGGCGCGCCAGTCGCTGC
AGGCCGCGGACTCCGGTCTGCAGGCCGTGCAGAACGCCATCGGCGGCATGAACTCCATCCGCGACCAGATCCAGGACACC
TCCAAGCGGATCAAGCGGCTGGGCGAATCGTCGCAGGAGATCGGTGAAATCACCGAGCTGATTTCCGACATTACCGAGCA
GACGAACGTGCTGGCACTGAACGCCGCCATCCAGGCCGCCTCGGCCGGCGAAGCGGGCCGCGGCTTCTCCGTCGTGGCGG
AAGAAGTGCAGCGCCTGGCCGAACGCTCCGCCGACGCCACGCGCCAGATCTCGGCGCTGGTGAAAGCCATTCAGACCGAC
ACGCAGGATGCGGTGGCCGCCATGGAGCGCTCCACGCAGGGTGTGGTGGAAGGCGCGCGCCTGTCCGACTCCGCCGGCAC
GGCCCTGACCGAGATCGACCGCGTGTCGCGGCGCCTCGCCGAGCTGATCGAGCAGATTTCCTCTTCCGCGTCGCGCGAGG
CCGAGCTGGCCAACGGCGTGGCCGACAACATCCAGCACATCTTCGCGGTGACCGAGCAGACCGGAGAAGGCACGCGCACC
ACGGCGCAGCAGGTGCGCGAACTGTCGCACATGGCCGAGGAGCTCCGCCAGTCGGTGGCGCGTTTCAAGATCGCCTGA

Upstream 100 bases:

>100_bases
GACCCCGTGGCAGGAGCTGAACCTGCAGGCGCTCTCCCAACACCCCGCATTCCTCAGCATCAGCGCTTGAGCGCACTCCC
TCTCTGAAGGCCGAACCATC

Downstream 100 bases:

>100_bases
CGGGACGGCCTGCAGCCAGCTAGCATCAACGAACGGCTCGCGAAGCCGGGGACGAATTCATGTCACCTATTGACGCCGCA
AACGCACCGGCCGCGGATTG

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MSVVNPFAKLFPRRTAGDASAGQDSLAAASYQSDGMQSVQGEPSALPPSPEGDFAEEDLISLPLLGRATAAAHQRRLLTL
LAVGVVVLALIAGWVLKQADRSAQQLAATGQALMQSQRLAKSVSQALVGSPQAFPDVVDSSGVLVRNVRALSSGDDDLGV
QALGEPFKPDLEAITPLMERAERNAGVVMGQQKILTQVGDALRTINRQSSDLLEIAETISSLKLQQNAPSTDISASGQLV
MLTQRIGKSANEFQTTEGVSPEAVFLLGKDLNSFREIAQGMLDGSSDLRLSATRDPQTREQLENLIKVYDQTRVQAGAIL
GNLQGLVSAREAQSAIIADSEPLRRQLEGLQTKLSGQTGLGAGQLAALVAAGLFVLLCGVGISRVQLMDSRTRQAAAEMQ
QRDARRQEQEAKRVNDANQAAILRLMNELQSVAEGDLTQEATVTEDITGAIADSVNYTVEELRQLVGSVQNTATRVAQTT
AQVDSTSTELLAASTEQLREIRETGRSVLDMATRINEVSAQAQESAAVARQSLQAADSGLQAVQNAIGGMNSIRDQIQDT
SKRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSADATRQISALVKAIQTD
TQDAVAAMERSTQGVVEGARLSDSAGTALTEIDRVSRRLAELIEQISSSASREAELANGVADNIQHIFAVTEQTGEGTRT
TAQQVRELSHMAEELRQSVARFKIA

Sequences:

>Translated_745_residues
MSVVNPFAKLFPRRTAGDASAGQDSLAAASYQSDGMQSVQGEPSALPPSPEGDFAEEDLISLPLLGRATAAAHQRRLLTL
LAVGVVVLALIAGWVLKQADRSAQQLAATGQALMQSQRLAKSVSQALVGSPQAFPDVVDSSGVLVRNVRALSSGDDDLGV
QALGEPFKPDLEAITPLMERAERNAGVVMGQQKILTQVGDALRTINRQSSDLLEIAETISSLKLQQNAPSTDISASGQLV
MLTQRIGKSANEFQTTEGVSPEAVFLLGKDLNSFREIAQGMLDGSSDLRLSATRDPQTREQLENLIKVYDQTRVQAGAIL
GNLQGLVSAREAQSAIIADSEPLRRQLEGLQTKLSGQTGLGAGQLAALVAAGLFVLLCGVGISRVQLMDSRTRQAAAEMQ
QRDARRQEQEAKRVNDANQAAILRLMNELQSVAEGDLTQEATVTEDITGAIADSVNYTVEELRQLVGSVQNTATRVAQTT
AQVDSTSTELLAASTEQLREIRETGRSVLDMATRINEVSAQAQESAAVARQSLQAADSGLQAVQNAIGGMNSIRDQIQDT
SKRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSADATRQISALVKAIQTD
TQDAVAAMERSTQGVVEGARLSDSAGTALTEIDRVSRRLAELIEQISSSASREAELANGVADNIQHIFAVTEQTGEGTRT
TAQQVRELSHMAEELRQSVARFKIA
>Mature_744_residues
SVVNPFAKLFPRRTAGDASAGQDSLAAASYQSDGMQSVQGEPSALPPSPEGDFAEEDLISLPLLGRATAAAHQRRLLTLL
AVGVVVLALIAGWVLKQADRSAQQLAATGQALMQSQRLAKSVSQALVGSPQAFPDVVDSSGVLVRNVRALSSGDDDLGVQ
ALGEPFKPDLEAITPLMERAERNAGVVMGQQKILTQVGDALRTINRQSSDLLEIAETISSLKLQQNAPSTDISASGQLVM
LTQRIGKSANEFQTTEGVSPEAVFLLGKDLNSFREIAQGMLDGSSDLRLSATRDPQTREQLENLIKVYDQTRVQAGAILG
NLQGLVSAREAQSAIIADSEPLRRQLEGLQTKLSGQTGLGAGQLAALVAAGLFVLLCGVGISRVQLMDSRTRQAAAEMQQ
RDARRQEQEAKRVNDANQAAILRLMNELQSVAEGDLTQEATVTEDITGAIADSVNYTVEELRQLVGSVQNTATRVAQTTA
QVDSTSTELLAASTEQLREIRETGRSVLDMATRINEVSAQAQESAAVARQSLQAADSGLQAVQNAIGGMNSIRDQIQDTS
KRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSADATRQISALVKAIQTDT
QDAVAAMERSTQGVVEGARLSDSAGTALTEIDRVSRRLAELIEQISSSASREAELANGVADNIQHIFAVTEQTGEGTRTT
AQQVRELSHMAEELRQSVARFKIA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=326, Percent_Identity=29.4478527607362, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788195, Length=323, Percent_Identity=28.7925696594427, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789453, Length=257, Percent_Identity=31.1284046692607, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787690, Length=286, Percent_Identity=29.7202797202797, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1788194, Length=189, Percent_Identity=32.8042328042328, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 79202; Mature: 79071

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVNPFAKLFPRRTAGDASAGQDSLAAASYQSDGMQSVQGEPSALPPSPEGDFAEEDLI
CCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHHHH
SLPLLGRATAAAHQRRLLTLLAVGVVVLALIAGWVLKQADRSAQQLAATGQALMQSQRLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSVSQALVGSPQAFPDVVDSSGVLVRNVRALSSGDDDLGVQALGEPFKPDLEAITPLMER
HHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHH
AERNAGVVMGQQKILTQVGDALRTINRQSSDLLEIAETISSLKLQQNAPSTDISASGQLV
HHCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MLTQRIGKSANEFQTTEGVSPEAVFLLGKDLNSFREIAQGMLDGSSDLRLSATRDPQTRE
EHHHHHCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHH
QLENLIKVYDQTRVQAGAILGNLQGLVSAREAQSAIIADSEPLRRQLEGLQTKLSGQTGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
GAGQLAALVAAGLFVLLCGVGISRVQLMDSRTRQAAAEMQQRDARRQEQEAKRVNDANQA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AILRLMNELQSVAEGDLTQEATVTEDITGAIADSVNYTVEELRQLVGSVQNTATRVAQTT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVDSTSTELLAASTEQLREIRETGRSVLDMATRINEVSAQAQESAAVARQSLQAADSGL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVQNAIGGMNSIRDQIQDTSKRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEHHHHHHHH
SAGEAGRGFSVVAEEVQRLAERSADATRQISALVKAIQTDTQDAVAAMERSTQGVVEGAR
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
LSDSAGTALTEIDRVSRRLAELIEQISSSASREAELANGVADNIQHIFAVTEQTGEGTRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TAQQVRELSHMAEELRQSVARFKIA
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVVNPFAKLFPRRTAGDASAGQDSLAAASYQSDGMQSVQGEPSALPPSPEGDFAEEDLI
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHHHH
SLPLLGRATAAAHQRRLLTLLAVGVVVLALIAGWVLKQADRSAQQLAATGQALMQSQRLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSVSQALVGSPQAFPDVVDSSGVLVRNVRALSSGDDDLGVQALGEPFKPDLEAITPLMER
HHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHH
AERNAGVVMGQQKILTQVGDALRTINRQSSDLLEIAETISSLKLQQNAPSTDISASGQLV
HHCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MLTQRIGKSANEFQTTEGVSPEAVFLLGKDLNSFREIAQGMLDGSSDLRLSATRDPQTRE
EHHHHHCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHH
QLENLIKVYDQTRVQAGAILGNLQGLVSAREAQSAIIADSEPLRRQLEGLQTKLSGQTGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
GAGQLAALVAAGLFVLLCGVGISRVQLMDSRTRQAAAEMQQRDARRQEQEAKRVNDANQA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AILRLMNELQSVAEGDLTQEATVTEDITGAIADSVNYTVEELRQLVGSVQNTATRVAQTT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVDSTSTELLAASTEQLREIRETGRSVLDMATRINEVSAQAQESAAVARQSLQAADSGL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVQNAIGGMNSIRDQIQDTSKRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEHHHHHHHH
SAGEAGRGFSVVAEEVQRLAERSADATRQISALVKAIQTDTQDAVAAMERSTQGVVEGAR
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
LSDSAGTALTEIDRVSRRLAELIEQISSSASREAELANGVADNIQHIFAVTEQTGEGTRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TAQQVRELSHMAEELRQSVARFKIA
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]